期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1823, 期 10, 页码 1731-1744出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2012.06.009
关键词
Adipogenesis; Connexin-43; Gap junction
资金
- Natural Sciences and Engineering Council of Canada
- Heart and Stroke Foundation of Manitoba
- Canada Research Chair Program
- Canada Foundation for Innovation for infrastructure support
Connexin-43 (Cx43) is a membrane phosphoprotein that mediates direct inter-cellular communication by forming gap junctions. In this way Cx43 can influence gene expression, differentiation and growth. Its role in adipogenesis, however, is poorly understood. In this study, we established that Cx43 becomes highly phosphorylated in early adipocyte differentiation and translocates to the plasma membrane from the endoplasmic reticulum. As preadipocytes differentiate, Cx43 phosphorylation declines, the protein is displaced from the plasma membrane, and total cellular levels are reduced via proteosomal degradation. Notably, we show that inhibiting Cx43 degradation or constitutively over-expressing Cx43 blocks adipocyte differentiation. These data reveal that transient activation of Cx43 via phosphorylation followed by its degradation is vital for preadipocyte differentiation and maturation of functional adipocytes. (c) 2012 Elsevier B.V. All rights reserved.
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