Editorial Material
Cell Biology
Liliana Felicia Iannucci, Giulietta Di Benedetto, Konstantinos Lefkimmiatis
Summary: Macroautophagy/autophagy is a cellular process responsible for eliminating and recycling aggregated proteins and damaged organelles. The compartmentalization of PRKA/PKA determines its effects on autophagy, with increased cAMP levels generating different PRKA activity signatures. The distribution of PRKA holoenzymes plays a role in affecting the autophagic flux in specific cell types.
Article
Microbiology
Xiaoqin Zhou, Jiangyong Li, Nianwu Tang, Hongyun Xie, Xiaoning Fan, Hui Chen, Ming Tang, Xianan Xie
Summary: AM fungi form a mutualistic symbiosis with terrestrial plants, utilizing multiple nutrient sensing and signaling pathways that may regulate gene expression and influence important developmental processes such as spore germination, appressorium formation, arbuscule longevity, and sporulation.
Article
Microbiology
Jin-Li Ding, Hai-Yan Lin, Jia Hou, Ming-Guang Feng, Sheng-Hua Ying
Summary: This study identified autophagy as a mechanism to safeguard conidial lifespans and vitality postmaturation, which can affect fungal dispersal in ecosystems and the biocontrol efficacy of entomopathogenic fungi during integrated pest management.
Article
Multidisciplinary Sciences
Juan Facundo Gulias, Florencia Niesi, Martin Aran, Susana Correa-Garcia, Mariana Bermudez-Moretti
Summary: Aging is a gradual decline in physiological integrity, which impairs functionality and increases mortality susceptibility. Dietary restriction, a known intervention to slow down aging, mimics nutrient scarcity without causing malnutrition. This study investigated the role of the transcription factor Gcn4 in the aging process of S. cerevisiae cells. The absence of Gcn4 led to various physiological changes, including increased mitochondrial activity and reactive oxygen species accumulation. Additionally, the mutant cells exhibited reduced ethanol production and lower levels of trehalose and free amino acids, disrupting cellular homeostasis and ultimately leading to a shortened lifespan.
Review
Biochemistry & Molecular Biology
Shree Padma Metur, Daniel J. Klionsky
Summary: Macroautophagy/autophagy is a crucial cellular process involved in cellular stress responses and maintaining cellular homeostasis. This review focuses on the current understanding of how cells regulate autophagy initiation through nutrient-dependent signaling pathways, which has significant implications for studying diseases.
Article
Biochemistry & Molecular Biology
Joichiro Nishio, Yoshiaki Takahashi, Masahiro Kasahara, Yoichi Takeda, Takashi Kikuma
Summary: A newly identified protein AeiA was found to be involved in selective autophagy and interacted with the autophagy-related protein Atg8 in Aspergillus oryzae. It was found to localize to autophagic membrane structures and peroxisomes. The disruption of AeiA resulted in reduced peroxisomal trafficking into the vacuole.
Review
Biochemistry & Molecular Biology
Michael Plank
Summary: TOR and PKA signaling pathways play important roles in growth regulation and nutrient sensing in Saccharomyces cerevisiae. This review explores the association between these pathways, focusing on shared target proteins. It discusses the potential direct regulation and cross-talk between TOR and PKA, as well as the transcriptional responses elicited by these pathways.
Article
Biochemistry & Molecular Biology
Sadiq Nasrah, Aline Radi, Johanna K. Daberkow, Helmut Hummler, Stefanie Weber, Elie Seaayfan, Martin Koemhoff
Summary: Melanoma-associated antigen D2 (MAGED2) activates the cAMP/PKA pathway under hypoxic conditions, stimulating renal salt reabsorption and explaining the transient variant of Bartter's syndrome. MAGED2 depletion leads to upregulation of autophagy-related genes and accumulation of autophagosome marker LC3II, indicating an inhibitory role of MAGED2 in autophagy under stress conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Eunbyeol Cho, Song Hee Lee, Ralph A. Dean, Junhyun Jeon
Summary: Studies have shown that in Magnaporthe oryzae, the size of the nucleolus may not necessarily correlate with its activity under low nutrient conditions. Initially, no or faint red fluorescent protein signals were observed in conidia, indicating the absence of functional nucleoli. However, signals started to appear within 2 hours of germination, reaching peak levels as the germ tube differentiated into the specialized infection structure, the appressorium.
Article
Biochemistry & Molecular Biology
A. Rehman Khalid, Shumin Zhang, Xiumei Luo, Hamayun Shaheen, Afshan Majeed, Mehdi Maqbool, Noosheen Zahid, Junaid Rahim, Maozhi Ren, Dan Qiu
Summary: The deletion mutant of the FoATG12 gene in the potato dry rot fungus Fusarium oxysporum exhibited reduced virulence and impaired aerial hyphae formation. This highlights the significant role of the FoATG12 gene in the pathogenicity and production of aerial hyphae in F. oxysporum, providing insights for pathogen control research and potential impacts on potato crops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Joshua D. D. Kerkaert, Lori B. B. Huberman
Summary: Organisms need to accurately sense and respond to nutrients for survival. Fungi have evolved genetic networks to regulate metabolic pathways and distinguish between preferred and nonpreferred nutrients. Transcription factors and regulatory genes play important roles in this regulation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Milton Packer
Summary: SGLT2 inhibitors promote cellular housekeeping and enhance cellular protection, leading to reduced progression of cardiomyopathy and nephropathy. Clinical studies suggest that SGLT2 inhibitors enhance nutrient deprivation signaling, resulting in a lower risk of heart failure and serious renal events.
Article
Agriculture, Multidisciplinary
Zhibing Luo, Qiyan Chen, Yufeng Su, Shasha Hu, Nemat O. Keyhani, Junyao Wang, Chenhua Zhu, Teng Zhou, Yunxia Pan, Michael J. Bidochka, Yongjun Zhang
Summary: The AreA GATA-type transcription factor plays a regulatory role in nitrogen catabolite repression, fungal development, and virulence in fungi. Knockdown of AreA gene expression in the entomopathogenic and plant endophyte Beauveria bassiana showed impairments in nitrogen utilization, increased sensitivities to osmotic stressors, but increased tolerances to oxidative/hypoxia stresses. The repression of AreA caused minimal effects on fungal virulence, mostly due to decreased host defense phenoloxidase activity, increased production of fungal metabolite oosporein, and impaired hyphal body development. Knockdown of AreA expression also resulted in impairment in the fungus's ability to associate with host plants.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biology
Yayong Yang, Bin Xie, Zhuohan Jing, Yuanping Lu, Jun Ye, Yizhao Chen, Fang Liu, Shaojie Li, Baogui Xie, Yongxin Tao
Summary: The study identified the Pkac gene in Flammulina filiformis, which is highly conserved in filamentous fungi and plays a role in mycelial growth and fruiting body development. Deletion of the Pkac gene resulted in slower growth of mycelia and significantly up-regulated expression under heat and oxidative stresses. The study revealed the conserved functions of PKAC in filamentous fungi and its regulatory mechanism in response to environmental stresses.
Article
Genetics & Heredity
Sheng-An Chen, Hung-Che Lin, Yen-Ping Hsueh
Summary: Nematode-trapping fungi use traps to capture and consume nematodes. This study shows that the cAMP-PKA signaling pathway and the G protein alpha subunit GPA2 are crucial for trap formation in Arthrobotrys oligospora.
G3-GENES GENOMES GENETICS
(2022)
Review
Cell Biology
Yasmina Filali-Mouncef, Catherine Hunter, Federica Roccio, Stavroula Zagkou, Nicolas Dupont, Charlotte Primard, Tassula Proikas-Cezanne, Fulvio Reggiori
Summary: Autophagic pathways intersect with lipid homeostasis to provide energy and building blocks essential for liver functions. Disruption of lipophagy regulation in fatty liver diseases, especially nonalcoholic steatohepatitis (NASH), may contribute to the development of hepatocellular carcinomas. This review focuses on the role of macroautophagy and macrolipophagy in NASH, highlighting the impact of inappropriate lipophagy on various types of liver cells.
Review
Cell Biology
Mariya Licheva, Babu Raman, Claudine Kraft, Fulvio Reggiori
Summary: Post-translational modifications play a crucial role in diversifying and coordinating protein networks in eukaryotic cells, particularly in processes like autophagy where the balance of kinases and phosphatases is essential. Autophagy, a highly conserved self-degradation process, is vital for survival, stress adaptation, and maintaining cellular and organismal homeostasis. Studies have highlighted the significance of kinases and phosphatases in regulating autophagy, targeting core autophagy proteins, and the relevance of phosphorylation for the overall process.
Article
Cell Biology
Idil Orhon, Cecilia Rocchi, Beatriz Villarejo-Zori, Paola Serrano Martinez, Mirjam Baanstra, Uilke Brouwer, Patricia Boya, Rob Coppes, Fulvio Reggiori
Summary: In dormant salivary gland stem cells, autophagy has a slower flux compared to self-renewing cells; however, enhancement of autophagy upon activation promotes self-renewal and tissue regeneration. Autophagy is a key pathway in activating self-renewal in low proliferative adult tissues, with potential for promoting tissue regeneration through pharmacological manipulation.
Article
Cell Biology
Rasha Khaddaj, Muriel Mari, Stephanie Cottier, Fulvio Reggiori, Roger Schneiter
Summary: This study investigates the localization of integral membrane proteins to the surface of lipid droplets (LDs) and confirms that the ER-LD junction acts as a barrier for ER-resident integral membrane proteins. The study also reveals that certain membrane proteins can be targeted to LDs through LD-targeted proteins.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Vikramjit Lahiri, Shree Padma Metur, Zehan Hu, Xinxin Song, Muriel Mari, Wayne D. Hawkins, Janakraj Bhattarai, Elizabeth Delorme-Axford, Fulvio Reggiori, Daolin Tang, Joern Dengjel, Daniel J. Klionsky
Summary: This study reveals that subtle changes in nutrient availability can have a significant impact on autophagy flux through unknown post-transcriptional regulatory mechanisms affecting the expression of key autophagy-inducing kinase. The identification of two novel post-transcriptional regulators further highlights the complexity of autophagy regulation.
Review
Biochemistry & Molecular Biology
Yan Hu, Fulvio Reggiori
Summary: Autophagy is a degradative process in eukaryotes that is crucial for maintaining cellular homeostasis and its defects are associated with various human diseases. The formation of autophagosomes, which sequester and degrade cellular materials, is a hallmark of autophagy. Recent advances in understanding the molecular regulation of autophagosome formation have been made, particularly in yeast and mammalian cells.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Review
Physiology
Fulvio Reggiori, Maurizio Molinari
Summary: ER-phagy is the process of degrading portions of the endoplasmic reticulum (ER) within lysosomes or vacuoles. It plays a role in recycling cytoplasmic material and organelles, regulating ER size and activity, and removing potentially cytotoxic material. Dysfunctional ER-phagy is associated with specific human diseases and can be targeted by pathogens.
PHYSIOLOGICAL REVIEWS
(2022)
Review
Cell Biology
Claudia Rio-Berge, Yingying Cong, Fulvio Reggiori
Summary: The cytoskeleton plays a crucial role in various physiological functions within the cell. Viruses can exploit the cytoskeleton by interacting with motor proteins to facilitate their own lifecycle. Understanding these interactions can provide insights into infection mechanisms and potential drug targets.
Review
Biochemistry & Molecular Biology
Mario Mauthe, Harm H. Kampinga, Mark S. Hipp, Fulvio Reggiori
Summary: Aggrephagy is the process of selective lysosomal transport and turnover of cytoplasmic protein aggregates by macro-autophagy. Protein aggregates are polyubiquitinated and sequestered by autophagosomes. Soluble selective autophagy receptors (SARs) play a central role in aggrephagy by physically binding to ubiquitin and the autophagy machinery. Understanding the mechanism of aggrephagy can potentially lead to therapeutic strategies for preventing the buildup of potentially toxic protein aggregates.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Cell Biology
Sabrina Chumpen Ramirez, Ruben Gomez-Sanchez, Pauline Verlhac, Ralph Hardenberg, Eleonora Margheritis, Katia Cosentino, Fulvio Reggiori, Christian Ungermann
Summary: During autophagy, Atg9 plays a critical role in establishing membrane contact sites and promoting lipid transfer. However, a specific mutation in Atg9 can impair autophagy progression by blocking phagophore expansion.
Article
Biochemistry & Molecular Biology
Ruheena Javed, Ashish Jain, Thabata Duque, Emily Hendrix, Masroor Ahmad Paddar, Sajjad Khan, Aurore Claude-Taupin, Jingyue Jia, Lee Allers, Fulong Wang, Michal Mudd, Graham Timmins, Keith Lidke, Tor Erik Rusten, Prithvi Reddy Akepati, Yi He, Fulvio Reggiori, Eeva-Liisa Eskelinen, Vojo Deretic
Summary: This study reveals that autophagosomal membranes become permeable and fail to mature into autolysosomes in cells lacking principal ATG8 proteins (mATG8s). It further uncovers a previously unknown function of mATG8s in maintaining the sealed state of autophagosomal membranes. The binding of mATG8 proteins GABARAP and LC3A to key ESCRT-I components contributes to the integrity and impermeability of autophagic membranes.
Article
Biochemistry & Molecular Biology
Henning Arlt, Babu Raman, Yasmina Filali-Mouncef, Yan Hu, Alexandre Leytens, Ralph Hardenberg, Rodrigo Guimaraes, Franziska Kriegenburg, Muriel Mari, Iwona I. Smaczynska-de Rooij, Kathryn R. Ayscough, Christian Ungermann, Joern Dengjel, Fulvio Reggiori
Summary: Autophagy is a crucial cellular process that maintains homeostasis by degrading cellular components. This study reveals that Vps1 mutants affect the subcellular distribution of Atg9 and impair autophagy. The findings provide new insights into the role of dynamins in Atg9 trafficking and their potential contribution to severe human pathologies associated with autophagy defects.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Hector Foronda, Yangxue Fu, Adriana Covarrubias-Pinto, Hartmut T. Bocker, Alexis Gonzalez, Eric Seemann, Patricia Franzka, Andrea Bock, Ramachandra M. M. Bhaskara, Lutz Liebmann, Marina E. E. Hoffmann, Istvan Katona, Nicole Koch, Joachim Weis, Ingo Kurth, Joseph G. G. Gleeson, Fulvio Reggiori, Gerhard Hummer, Michael M. M. Kessels, Britta Qualmann, Muriel Mari, Ivan Dikic, Christian A. A. Huebner
Summary: Membrane-shaping proteins containing reticulon homology domains are crucial for dynamic remodelling of the endoplasmic reticulum (ER). FAM134B is an example of such a protein, which mediates the degradation of ER sheets through a process called selective autophagy (ER-phagy) by binding to LC3 proteins. Mutations in FAM134B result in a neurodegenerative disorder in humans.
Review
Biochemistry & Molecular Biology
Claudine Kraft, Fulvio Reggiori
Summary: This review discusses three membrane remodeling steps in autophagy: closure of phagophores, maturation of autophagosomes, and fusion with vacuoles/lysosomes. The focus is on the role and contribution of Saccharomyces cerevisiae in studying molecular events in autophagy.
Article
Immunology
Chairi Misrielal, Astrid M. Alsema, Marion H. C. Wijering, Anneke Miedema, Mario Mauthe, Fulvio Reggiori, Bart J. L. Eggen
Summary: Autophagy plays a crucial role in different stages of multiple sclerosis (MS) pathology, with decreased expression of autophagy-related genes observed in the acute phase of MS and postmortem brain tissues. The negative regulation of autophagy in MS may be mediated through persistent activation of the mTORC1 pathway, affecting CNS homeostasis and neuroinflammation in MS.
BRAIN, BEHAVIOR, & IMMUNITY - HEALTH
(2022)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)