Article
Biochemistry & Molecular Biology
Wenjie Zhou, Binhan Hao, Terry M. Bricker, Steven M. Theg
Summary: The twin arginine translocation (Tat) pathway plays an important role in protein transport in bacteria, archaea, and plants. By using a real-time luminescence assay, this study reveals that both Delta psi and Delta pH contribute energetically to Tat transport in Escherichia coli spheroplasts.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Sam Wilson, Dan-Hong Li, Alexander Ruban
Summary: The study reveals pseudothylakoid characteristics in artificial LHCII membranes, mimicking natural plant membranes. High densities of LHCII cause liposome stacking and the appearance of an unexpected emission peak similar to photosystem I emission. These findings have significant implications for interpreting the spectroscopic and physiological properties of photosynthetic membranes.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biophysics
Fatemeh Azadi-Chegeni, Meaghan E. Ward, Giorgio Perin, Diana Simionato, Tomas Morosinotto, Marc Baldus, Anjali Pandit
Summary: This study demonstrates the dynamic conformational transitions of photosynthetic light-harvesting complexes in different environments using solid-state NMR spectroscopy. Results show that LHCII in lipid bilayers undergoes fast, large-amplitude motions involving specific sites, including chlorophylls and protein fragments. The study also suggests that in thylakoid membranes, the dynamics of protein and pigment sites are significantly reduced compared to the membrane environment, indicating a specific conformational state for LHCII complexes.
BIOPHYSICAL JOURNAL
(2021)
Article
Chemistry, Physical
Maksym Golub, Heiko Lokstein, Dmytro Soloviov, Alexander Kuklin, D. C. Florian Wieland, Jorg Pieper
Summary: In this study, small-angle scattering was used to investigate the solution structures of solubilized LHCII. The results showed that the solution structures of LHCII may differ under different preparation conditions, with the LHCII-beta-DM solution revealing a stable oligomer structure that may play a crucial role in photoprotective nonphotochemical quenching.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Multidisciplinary Sciences
Omar Sandoval-Ibanez, David Rolo, Rabea Ghandour, Alexander P. Hertle, Tegan Armarego-Marriott, Arun Sampathkumar, Reimo Zoschke, Ralph Bock
Summary: The DEIP1 protein interacts with the PetA and PetB subunits and plays an essential role in the assembly of the Cytb6f complex. This finding provides insights into the assembly pathway of a crucial complex in photosynthesis.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Tamas Yelland, Esther Garcia, Charles Parry, Dominika Kowalczyk, Marta Wojnowska, Andrea Gohlke, Matja Zalar, Kenneth Cameron, Gillian Goodwin, Qing Yu, Peng-Cheng Zhu, Yasmin ElMaghloob, Angelo Pugliese, Lewis Archibald, Andrew Jamieson, Yong Xiang Chen, Duncan McArthur, Justin Bower, Shehab Ismail
Summary: This study presents a novel strategy for targeting RAS by stabilizing its interaction with PDE6D and disrupting its localization. By using designed RAS point mutations, the RAS:PDE6D complex is stabilized, leading to inhibition of oncogenic RAS/ERIC signaling. Additionally, the impact of this strategy appears to be cell-type-dependent.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Lvyuan Fan, Zhiyu Pan, Yilin Zhong, Juan Guo, Xu Liao, Rui Pang, Qingqiang Xu, Guozhu Ye, Yubin Su
Summary: This study identified a promising therapeutic strategy for treating infections caused by drug-resistant Gram-positive bacteria. By combining metabolites and antibiotics, the killing efficacy of gentamicin was enhanced and the survival rate of infected mice was improved.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biophysics
Fabian Giska, Malaiyalam Mariappan, Moitrayee Bhattacharyya, Kallol Gupta
Summary: In this study, the authors used native mass spectrometry (Native-MS) to investigate the oligomeric states and structural organization of the Get3/4/5 chaperone complex. They discovered that Get4/5 forms a tetramer and interacts with Get4/4 through a novel dimerization interface. Addition of Get3 results in the formation of a hexameric complex with a closed-ring cyclic architecture. These findings provide molecular-level insights and demonstrate the importance of native-MS in studying large multiprotein complexes.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Zhen Xiao, Chengcheng Huang, Haitao Ge, Yan Wang, Xiaoxiao Duan, Gaojie Wang, Limin Zheng, Jinghui Dong, Xiahe Huang, Yuanya Zhang, Hongyu An, Wu Xu, Yingchun Wang
Summary: Using APEX2-based proximity labeling, the study identified proteins proximal to the photosystem II oxygen evolution complex (OEC) in a cyanobacterium model. Several proteins involved in PS II assembly, maturation, and repair were discovered, along with potential regulators of PS II structure and function. This study provides valuable insights into the proteome neighborhood and regulation of a key protein complex.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Mark Helamieh, Marco Reich, Philipp Rohne, Ulf Riebesell, Martin Kerner, Klaus Kuemmerer
Summary: The spectral composition of light affects the metabolism of photosynthetic organisms. This study found that blue-green light promotes the growth and pigment concentration in Monoraphidium braunii, but red light also contributes to high biomass production.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2023)
Review
Plant Sciences
B. K. Semin, L. N. Davletshina, A. V. Loktyushkin, E. R. Lovyagina
Summary: Photosystem II (PSII) in oxygenic organisms plays a crucial role in converting light energy into molecular oxygen. The discovery of a structural transition in the catalytic center of PSII at pH 5.7 has significant implications for understanding the defense mechanisms against photoinhibition and the overall efficiency of PSII.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Cell Biology
Tom Cremer, Marlieke L. M. Jongsma, Fredrik Trulsson, Alfred C. O. Vertegaal, Jacques Neefjes, Ilana Berlin
Summary: The study reveals that ER-associated protein complex can influence the positioning and transport of endolysosomes, thereby regulating signal transduction within the cell. This regulation is achieved through specific protein modifications, facilitating the termination of signaling pathways.
Article
Biochemistry & Molecular Biology
Binhan Hao, Wenjie Zhou, Steven M. Theg
Summary: The Tat pathway facilitates the transport of folded proteins across various membranes, and the polar amino acid at the N terminus of the TatA transmembrane helix was thought to be crucial. However, our experimental results in Escherichia coli showed that the polar amino acid is not necessary for TatA's functionality. Instead, the hydrophilicity and helix-stabilizing properties of this amino acid were found to strongly correlate with Tat activity. Additionally, we identified a four-residue motif at the N terminus of TatA's transmembrane helix, which may play a role in the preference for different proton-motive force components.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Daming Zhu, Weili Dai, Prakash Srinivasan, Holly McClellan, Dennis Braden, Alec Allee-Munoz, Patricia A. Gonzales Hurtado, Louis H. Miller, Patrick E. Duffy
Summary: The AMA1-RON2L complex is a promising candidate for a malaria vaccine, but careful assessment is needed to determine its identity and stability. Using Tris-glycine gel separation, Western blotting with mass spectrometry, and cIEF techniques, the complex was successfully identified and confirmed, showing stability for up to 72 hours at 4 degrees C.
Article
Cell Biology
Esra Peker, Konstantin Weiss, Jiyao Song, Christine Zarges, Sarah Gerlich, Volker Boehm, Aleksandra Trifunovic, Thomas Langer, Niels H. Gehring, Thomas Becker, Jan Riemer
Summary: Peker et al. discovered a two-step import pathway that allows proteins to be localized to both the matrix and IMS. Weak targeting signals enable proteins to form stabilizing disulfide bonds in the IMS before being imported into the matrix. This pathway enables the monitoring of import activity in both compartments. The study found that NDUFAF8, a factor involved in complex I assembly, follows this two-step import pathway, and its import is regulated by proteases to ensure proper function.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)