Article
Biology
Maria del Mar Gil-Sanchez, Sara Cea-Sanchez, Eva M. Luque, David Canovas, Luis M. Corrochano
Summary: This study describes a novel effect of light regulation on the degradation of VE-1, a key component of the velvet complex in fungi. The accumulation of VE-1 under light exposure allows the fungal cell to adapt quickly to changes in light and regulate the expression of genes involved in photoprotective pigment biosynthesis.
Article
Endocrinology & Metabolism
William E. Samsa, Murali K. Mamidi, Bryan S. Hausman, Lindsay A. Bashur, Edward M. Greenfield, Guang Zhou
Summary: This study investigated the specific role of Jab1 in osteoblast differentiation and postnatal bone growth using a novel mouse model. The results showed that Jab1 is essential for proper osteoblast differentiation and postnatal bone growth, likely through its positive regulation of the TGF beta and BMP signaling pathways in osteoblast progenitor cells. Knockout of Jab1 led to progressive dwarfism, altered bone microarchitecture, reduced mineralization, and decreased response to TGF beta and BMP signaling, ultimately resulting in spontaneous fractures and premature death in mutant mice.
Article
Agronomy
Elshan Musazade, Yanxi Liu, Yixuan Ren, Ming Wu, Hua Zeng, Shining Han, Xiaowei Gao, Shuhua Chen, Liquan Guo
Summary: The COP9 signalosome (CSN), particularly CSN1, plays a crucial role in plant development. This study focused on investigating the role of CSN1 in rice using the CRISPR/Cas9 system. The results showed that OsCSN1 acts as a negative regulator in seedling growth and development by promoting the degradation of the SLR1 protein in the GA signaling pathway.
Article
Multidisciplinary Sciences
Dawadschargal Dubiel, Jing Wang, Roland Hartig, Supattra Chaithongyot, Wolfgang Daniel, Michael Naumann
Summary: The COP9 signalosome and cullin-RING ubiquitin ligases form latent CSN-CRL complexes detectable in cells. CSN7A and CSN7B variants preferentially bind to CRL3 and CRL4A/B, respectively. Specific cellular functions require latent CSN7A-CRL3 and CSN7B-CRL4A/B particles, with curcumin-induced cell death requiring latent CSN7B-CRL4A.
Article
Multidisciplinary Sciences
Hong Lin, Yuan Yan, Yifan Luo, Wing Yan So, Xiayun Wei, Xiaozhe Zhang, Xiaoli Yang, Jun Zhang, Yang Su, Xiuyan Yang, Bobo Zhang, Kangjun Zhang, Nan Jiang, Billy Kwok Chong Chow, Weiping Han, Fengchao Wang, Feng Rao
Summary: The study uncovers an antagonistic relationship between COP1 and CSN in glucose-induced insulin secretion, with disruption of the CRL4(COP1)-ETV5 proteolytic axis leading to hyperinsulinemia and obesity. The findings suggest that deregulation of the IP6-CSN-CRL4(COP1)-ETV5 axis underlies these pathologies, and that targeting this pathway could reduce the risk of obesity and diabetes. Furthermore, inhibition of CRL neddylation can correct these pathological phenotypes.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Dong-Min Gao, Ji-Hui Qiao, Qiang Gao, Jiawen Zhang, Ying Zang, Liang Xie, Yan Zhang, Ying Wang, Jingyan Fu, Hua Zhang, Chenggui Han, Xian-Bing Wang
Summary: This study reveals that the barley yellow striate mosaic virus infects the central nervous system of its insect vector, leading to hyperactivity and prolonged feeding duration. The virus's accessory protein P6 interacts with a specific subunit of the insect vector, inhibiting the degradation of the circadian clock protein Timeless. Furthermore, expression of P6 in fruit flies disrupts circadian rhythm and induces high locomotor activity.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Murali K. Mamidi, William E. Samsa, Lindsay A. Bashur, Yuqing Chen, Ricky Chan, Brendan Lee, Guang Zhou
Summary: The transcriptional cofactor Jab1/Cops5 interacts with key factors in chondrocytes, including Smad1/5/8 and p53, regulating BMP signaling and apoptosis pathways. Proper Jab1 expression is crucial for maintaining chondrocyte survival and differentiation, as well as skeletal development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yaru Zhang, Marco Jost, Ryan A. Pak, Daniel Lu, Jing Li, Brett Lomenick, Spiros D. Garbis, Chi-Ming Li, Jonathan S. Weissman, James Russell Lipford, Raymond J. Deshaies
Summary: Cop9 signalosome (CSN) regulates the function of cullin-RING E3 ubiquitin ligases (CRLs) by deconjugating the ubiquitin-like protein NEDD8 from the cullin subunit. In this study, quantitative mass spectrometry and genome-wide CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) screens were used to identify factors that modulate cell viability upon inhibition of CSN. The study found that CRL components and regulators strongly modulate the antiproliferative effects of CSN inhibition, and identified two pathways involved in genome integrity that contribute to the toxicity of CSN inhibition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Seyed Mahdi Hosseiniyan Khatibi, Mohammadreza Ardalan, Mohammad Teshnehlab, Sepideh Zununi Vahed, Saeed Pirmoradi
Summary: This study identified a set of mRNA and miRNA panels that can distinguish between different subtypes of renal cell carcinoma (RCC), providing new insights into the underlying mechanisms of initiation and progression of RCC.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Murali K. Mamidi, William E. Samsa, David Danielpour, Ricky Chan, Guang Zhou
Summary: The study reveals a novel role of JAB1 in chondrosarcoma, showing that knockdown of JAB1 significantly reduces cell proliferation and increases apoptosis. JAB1 interacts with SOX9, regulates specific pathways and CRLs in chondrosarcoma cells.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Microbiology
Zi-Fang Shen, Lin Li, Jing-Yi Wang, Yun-Ran Zhang, Zi-He Wang, Shuang Liang, Xue-Ming Zhu, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu
Summary: The COP9 signalosome (CSN) plays an important role in regulating fungal development, pathogenicity, and autophagy in Magnaporthe oryzae. The CSN subunit MoCsn6 is involved in vegetative growth, sporulation, and plant infection. Deletion of MoCSN6 disrupts the Osm1 and Pmk1 phosphorylation pathways and negatively regulates autophagic activity by interacting with MoAtg6 and controlling its ubiquitination level.
MICROBIOLOGY SPECTRUM
(2022)
Review
Physiology
William D. Kim, Sabateeshan Mathavarajah, Robert J. Huber
Summary: Cullins are important for regulating protein degradation and function. The social amoeba Dictyostelium discoideum is a valuable model organism for studying cellular processes and diseases. Recent research shows that inhibiting neddylation affects the growth and development of D. discoideum.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Phatcharida Jantaree, Supattra Chaithongyot, Olga Sokolova, Michael Naumann
Summary: Helicobacter pylori infection is associated with the development of gastric diseases, including cancer. NF-κB and DUB USP48 play important roles in this process. USP48 stabilizes NF-κB activity by deubiquitination, enhancing its transcriptional activity and reducing apoptotic cell death. This finding provides new insights into the cell survival mechanism in H. pylori infection.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jayson Sia, Wei Zhang, Edmond Jonckheere, David Cook, Paul Bogdan
Summary: Cellular biological networks play a crucial role in regulating the functions of living cells. This article introduces a geometric-based detection framework, utilizing network topology information, to accurately detect communities within partially observed biological networks. The integration of gene function knowledge improves the accuracy in detecting essential conserved and varied biological communities. The results demonstrate that this approach can uncover novel components and communities, providing valuable insights into the organization and function of complex networks.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Ozlem Sarikaya Bayram, Ozgur Bayram, Betim Karahoda, Cindy Meister, Anna M. Kohler, Sabine Thieme, Nadia Elramli, Dean Frawley, Jamie McGowan, David A. Fitzpatrick, Kerstin Schmitt, Leandro Jose de Assis, Oliver Valerius, Gustavo H. Goldman, Gerhard H. Braus
Summary: F-box proteins play important roles in coordinating fungal development and secondary metabolism by controlling subcellular accumulations and degradation of key regulatory proteins.
Article
Cardiac & Cardiovascular Systems
Melanie Bezard, Mounira Kharoubi, Arnault Galat, Elsa Poullot, Soulef Guendouz, Pascale Fanen, Benoit Funalot, Anissa Moktefi, Jean-Pascal Lefaucheur, Mukedaisi Abulizi, Jean-Francois Deux, Thierry Gendre, Vincent Audard, Khalil el Karoui, Florence Canoui-Poitrine, Amira Zaroui, Emmanuel Itti, Emmanuel Teiger, Violaine Plante-Bordeneuve, Silvia Oghina, Thibaud Damy
Summary: This study found an association between tafamidis treatment and a lower occurrence of cardiovascular outcomes in a real-life population of ATTRwt and ATTRv patients representative of the inclusion criteria of the ATTR-ACT trial.
EUROPEAN JOURNAL OF HEART FAILURE
(2021)
Article
Allergy
Malek Nefzi, Imen Wahabi, Sondess Hadj Fredj, Rym Othmani, Rym Dabboubi, Khedija Boussetta, Pascale Fanen, Taieb Messaoud
Summary: This study describes the molecular variability in the SFTPC gene in childhood asthma in the Tunisian population. The T138N polymorphism shows a significant association with asthma, and the 138Asn-186Ser and 138Thr-186Asn haplotypes have a negative association with the disease. This research highlights the genetic variability of the SFTPC gene and its potential role in asthma in Tunisia.
Article
Pharmacology & Pharmacy
Celine Delestrain, Abdel Aissat, Stephanie Simon, Agathe Tarze, Elodie Duprat, Elodie Nattes, Bruno Costes, Valerie Delattre, Stephanie Finet, Pascale Fanen, Ralph Epaud
Summary: Twins with SP-C gene mutation underwent successful GC treatment, leading to improved proSP-C processing in BALF analysis and a decrease in SFTPC mRNA expression with splicing changes, resulting in the expression of a less stable proSP-C isoform.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Respiratory System
Natacha Martin, Anne Bergougnoux, Nesrine Baatallah, Benoit Chevalier, Jessica Varilh, David Baux, Bruno Costes, Pascale Fanen, Caroline Raynal, Isabelle Sermet-Gaudelus, Emmanuelle Girodon, Magali Taulan-Cadars, Alexandre Hinzpeter
Summary: This study identified 26 out of 65 CFTR variants that enhanced exon skipping, with in silico prediction efficacy ranging from 50% to 66%. Classification of exons based on their basal exon skipping level improved prediction rates up to 80%.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Letter
Hematology
Lamisse Mansour-Hendili, Stephane Egee, David Monedero-Alonso, Guillaume Bouyer, Bertrand Godeau, Bouchra Badaoui, Ariane Lunati, Clara Noizat, Abdelrazak Aissat, Laurent Kiger, Chadia Mekki, Veronique Picard, Stephane Moutereau, Pascale Fanen, Pablo Bartolucci, Loic Garcon, Frederic Galacteros, Benoit Funalot
AMERICAN JOURNAL OF HEMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Stephanie Simon, Abdel Aissat, Fanny Degrugillier, Benjamin Simonneau, Pascale Fanen, Andre-Patrick Arrigo
Summary: Human small heat shock proteins, including HspB1, HspB4, and HspB5, play crucial roles in regulating cellular processes related to Cystic Fibrosis (CF). While HspB1 and HspB4 promote the degradation of CFTR mutants, HspB5 enhances the transport and function of CFTR mutant at the plasma membrane. Additionally, HspB5 molecules can increase the cellular efficiency of current CF therapeutic molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Chadia Mekki, Abdel Aissat, Veronique Mirlesse, Sophie Mayer Lacrosniere, Elsa Eche, Annick Le Floch, Sandra Whalen, Cecile Prud'Homme, Christelle Remus, Benoit Funalot, Vanina Castaigne, Pascale Fanen, Alix de Becdelievre
Summary: This study shows that fetal ultrasound bowel abnormalities can unexpectedly reveal cystic fibrosis (CF) in families without a CF history. A comprehensive study of the CFTR gene is recommended for cases with bowel abnormalities to identify potential mutations, even in populations without well-known frequent pathogenic variants.
Article
Biochemistry & Molecular Biology
Marion Onnee, Pascale Fanen, Isabelle Callebaut, Alix de Becdelievre
Summary: ABCA3 protein plays a crucial role in pulmonary surfactant biosynthesis and is associated with recessive pulmonary disorders. Using 3D structure information, researchers modeled the protein and pinpointed known pathogenic missense variants, providing insights into the possible impact of these variants in the protein's structure and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Louis Bonnefous, Mounira Kharoubi, Melanie Bezard, Silvia Oghina, Fabien Le Bras, Elsa Poullot, Valerie Molinier-Frenkel, Pascale Fanen, Jean-Francois Deux, Vincent Audard, Emmanuel Itti, Thibaud Damy, Etienne Audureau
Summary: Clustering analysis identified 7 distinct clinical profiles among suspected CA patients, indicating areas for improvement in amyloidosis diagnosis and prognosis stratification based on associated risk factors.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2021)
Article
Medicine, General & Internal
Silvia Oghina, Wulfran Bougouin, Mounira Kharoubi, Louis Bonnefous, Arnault Galat, Soulef Guendouz, Melanie Bezard, Fabien Le Bras, Jean-Francois Deux, Emmanuel Itti, Anissa Moktefi, Pascale Fanen, Emmanuel Teiger, Dania Mohty, Thibaud Damy, Diane Bodez
Summary: All LVDF patterns can be observed in CA, and one quarter of CA patients have a better prognosis with grade I LVDF.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, General & Internal
Silvia Oghina, Constant Josse, Melanie Bezard, Mounira Kharoubi, Marc-Antoine Delbarre, Damien Eyharts, Amira Zaroui, Soulef Guendouz, Arnault Galat, Luc Hittinger, Pascale Fanen, Emmanuel Teiger, Nadir Mouri, Francois Montestruc, Thibaud Damy
Summary: The study assessed the evolution and prognostic value of NT-proBNP and cTnT-HS in ATTR-CA patients before and after tafamidis treatment. The results showed that patients with increasing NT-proBNP had an increased risk of adverse events, but tafamidis treatment could stabilize these biomarker levels.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Antoine Jobbe-Duval, Melanie Bezard, Stephane Moutereau, Mounira Kharoubi, Silvia Oghina, Amira Zaroui, Arnault Galat, Coraline Chalard, Elisabeth Hugon-Vallet, Francois Lemonnier, Damien Eyharts, Elsa Poulot, Pascale Fanen, Benoit Funalot, Valerie Molinier-Frenkel, Vincent Audard, Luc Hittinger, Marc Antoine Delbarre, Emmanuel Teiger, Thibaud Damy
Summary: Iron deficiency is common in patients with cardiac amyloidosis, regardless of subtype. Patients are more likely to have iron deficiency if diagnosed with ATTR, diabetic, and/or treated with aspirin. The benefit of intravenous iron therapy for iron deficiency in cardiac amyloidosis needs further study on morbidity and mortality.
Article
Cardiac & Cardiovascular Systems
Mounira Kharoubi, Melanie Bezard, Arnault Galat, Fabien Le Bras, Elsa Poullot, Valerie Molinier-Frenkel, Pascale Fanen, Benoit Funalot, Anissa Moktefi, Jean-Pascal Lefaucheur, Mukedaisi Abulizi, Jean-Francois Deux, Francois Lemonnier, Soulef Guendouz, Coraline Chalard, Amira Zaroui, Vincent Audard, Emilie Bequignon, Diane Bodez, Emmanuel Itti, Luc Hittinger, Etienne Audureau, Emmanuel Teiger, Silvia Oghina, Thibaud Damy
Summary: This study reveals the importance of amyloidosis type and progression in diagnostic delay and prognosis, highlighting the need for physicians to remain vigilant towards extracardiac and cardiac events for significantly improving early diagnosis of amyloidosis.
Letter
Biochemistry & Molecular Biology
Marion Onnee, Benedicte Duriez, Stephanie Simon, Claire Szczepaniak, Aurelie Guguin, Ralph Epaud, Samia Hamouda, Pascale Fanen, Alix de Becdelievere
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Mounira Kharoubi, Melanie Bezard, Arnault Galat, Fabien Le Bras, Elsa Poullot, Valerie Molinier-Frenkel, Pascale Fanen, Benoit Funalot, Anissa Moktefi, Jean-Pascal Lefaucheur, Mukedaisi Abulizi, Jean-Francois Deux, Francois Lemonnier, Soulef Guendouz, Coraline Chalard, Amira Zaroui, Vincent Audard, Emilie Bequignon, Diane Bodez, Emmanuel Itti, Luc Hittinger, Etienne Audureau, Emmanuel Teiger, Silvia Oghina, Thibaud Damy
Summary: This study compared AECE and ACE between different types of CA, finding that the age of onset for the first AECE and ACE was lower for AL and ATTRv patients compared to ATTRwt patients. In AL and ATTRwt, a shorter delay between the onset of the first AECE or ACE and diagnosis was associated with reduced survival rate.
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
