期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1831, 期 4, 页码 675-682出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2012.12.010
关键词
The oleaginous yeast; Yarrowia lipolytica; Lipid body; Oil accumulation; Gene expression
资金
- National Natural Science Foundation of China [31070029]
- Hi-Tech Research and Development Program of China (863) [2012AA021205]
In this study, the MIG1 gene in the oleaginous yeast Yarrowia lipolytica ACA-DC 50109 (the parent yeast) was disrupted and the disruptant M25 obtained could grow in yeast nitrogen base-N-5000 medium without uracil or the medium with 2-deoxy-D-glucose. It was found that the cells of the disruptant M25 had more lipid bodies than those of its parent yeast. The disruptant M25 contained 48.7% (w/w) of oil based on its cell weight while the parent yeast only contained 36.0% (w/w) of oil. Transcript levels of many genes relevant to lipid biosynthesis in the disruptant M25 were enhanced compared to those of the same genes in the parent yeast. However, transcript level of the MFE1 gene, one of the genes relevant to fatty acid degradation was reduced in the disruptant M25 compared to that of the same gene in the parent yeast. Such changes in gene expression profile may cause the increased lipid biosynthesis in the disruptant M25. Biosynthesis of C-18:1 fatty acid in the disruptant M25 was greatly enhanced compared to that in the parent yeast. (C) 2012 Elsevier B.V. All rights reserved.
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