期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1830, 期 9, 页码 4359-4364出版社
ELSEVIER
DOI: 10.1016/j.bbagen.2013.03.004
关键词
Field effect transistors; Glucose; Phenylboronic acids; Heat tolerance
资金
- Japan Science and Technology Agency (JST)
- Core Research of Evolutional Science & Technology (CREST)
- Japan Society for the Promotion of Science (JSPS)
- Council for Science and Technology Policy (CSTP)
- Nano-Integration Facility at the National Institute for Materials Science (NIMS)
- Grants-in-Aid for Scientific Research [23680051] Funding Source: KAKEN
Background: Field effect transistor (PET) based signal-transduction (Bio-FET) is an emerging technique for label-free and real-time basis biosensors for a wide range of targets. Glucose has constantly been of interest due to its clinical relevance. Use of glucose oxidase (GOD) and a lectin protein Concanavalin A are two common strategies to generate glucose-dependent electrochemical events. However, these protein-based materials are intolerant of long-term usage and storage due to their inevitable denaturing. Methods: A phenylboronic acid (PEA) modified self-assembled monolayer (SAM) on a gold electrode with an optimized disassociation constant of PBA, that is, 3-fluoro-4-carbamoyl-PBA possessing its pKa of 7.1, was prepared and utilized as an extended gate electrode for Bio-FET. Results: The prepared electrode showed a glucose-dependent change in the surface potential under physiological conditions, thus providing a remarkably simple rationale for the glyco-sensitive Bio-FET. Importantly, the PBA modified electrode showed tolerance to relatively severe heat and drying treatments; conditions under which protein based materials would surely be denatured. Conclusions: A PEA modified SAM with optimized disassociation constant (pKa) can exhibit a glucose-dependent change in the surface potential under physiological conditions, providing a remarkably simple but robust method for the glyco-sensing. General significance: This protein-free, totally synthetic glyco-sensing strategy may offer cheap, robust and easily accessible platform that may be useful in developing countries. This article is part of a Special Issue entitled Organic Bioelectronics-Novel Applications in Biomedicine. (C) 2013 Elsevier B.V. All rights reserved.
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