期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
卷 1839, 期 6, 页码 463-475出版社
ELSEVIER
DOI: 10.1016/j.bbagrm.2014.04.006
关键词
piRNAs; Endo-siRNA; Deep sequencing; Transposable element; Germ cell; Zygote
资金
- European Chemical Industry Council Long-range Research Initiative (CEFIC-LRi) [LRI-EMSG49]
- MEDDTL, France [11-MRES-PNRPE-9-CVS-072]
- CSIC, Spain [PIE 201020E016]
A set of small RNAs known as rasRNAs (repeat-associated small RNAs) have been related to the down-regulation of Transposable Elements (TEs) to safeguard genome integrity. Two key members of the rasRNAs group are piRNAs and endo-siRNAs. We have performed a comparative analysis of piRNAs and endo-siRNAs present in mouse oocytes, spermatozoa and zygotes, identified by deep sequencing and bioinformatic analysis. The detection of piRNAs and endo-siRNAs in the spermatozoa and revealed also in zygotes, hints to their potential delivery to oocytes during fertilization. However, a comparative assessment of the three cell types indicates that both piRNAs and endo-siRNAs are mainly maternally inherited. Finally, we have assessed the role of the different rasRNA molecules in connection with amplification processes by way of the ping-pong cycle. Our results suggest that the ping-pong cycle can act on other rasRNAs, such as tRNA- and rRNA-derived fragments, thus not only being restricted to TEs during gametogenesis. (C) 2014 Elsevier B.V. All rights reserved.
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