Review
Chemistry, Analytical
Qianying Sheng, Meiyan Liu, Minbo Lan, Guangyan Qing
Summary: Hydrophilic interaction liquid chromatography (HILIC) is an attractive complementary method to reversed phase LC for the separation of hydrophilic or strongly polar substances. The advancements in HILIC have significantly improved sensitivity, analyte coverage, throughput, analysis speed, and resolution in bio-separation and bio-analytical chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Yong Guo
Summary: Various polar stationary phases have been extensively used in HILIC applications in the past decade. This review provides an overview of commercially available polar stationary phases and summarizes the progress made in the past 5 years. New types of stationary phases are discussed, and the trend in stationary phase research and development is highlighted. The review also emphasizes the experimental evidence for direct interactions between polar analytes and stationary phase ligands in HILIC conditions, as well as the advancements in understanding the retention mechanisms.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Biochemical Research Methods
Xiaoqing Wang, Jian Cui, Jin Zhou, Shuo Wang, Yiming Gu, Xiaowei Liu, Shudong Wang
Summary: Vinyl modified silica was synthesized through a silane coupling reaction and a poly-acrylamide modified silica (PAM-SIL) stationary phase was prepared via free radical polymerization. The separation performance of the stationary phases could be regulated by controlling the polymer structure, showing potential for future separation and analysis applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Isabelle Kohler, Michel Verhoeven, Rob Haselberg, Andrea F. G. Gargano
Summary: Proteomics and metabolomics have become increasingly important in clinical applications, with RPLC-MS and HILIC-MS techniques playing crucial roles in biomarker discovery and disease diagnosis. While RPLC-MS and HILIC-MS complement each other in resolving the complexity of proteome and metabolites, they also have technical limitations and potential solutions that need to be addressed.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Fei Wang, Fan Yang, Jiawei Liu, Quan Bai
Summary: This study investigates the retention behavior and mechanism of solutes in hydrophilic interaction chromatography (HILIC) using stoichiometric displacement theory (SDT). A HILIC/reversed-phase liquid chromatography (RPLC) dual-retention mechanism is studied in detail using a β-CD HILIC column. The study finds that a four-parameter equation derived from SDT-R accurately describes the U-shaped curves of solutes with RPLC/HILIC dual-retention mechanisms on the β-CD column, providing a theoretical guide for the development of HILIC and the enhancement of separation efficiency.
Article
Biochemical Research Methods
Haibin Li, Chusheng Liu, Li Zhao, Dongsheng Xu, Tingting Zhang, Qiqin Wang, Deirdre Cabooter, Zhengjin Jiang
Summary: An unmatched or improperly matched sample solvent can distort the peak shape of analytes in chromatography. For retained compounds, pure ACN is the best sample solvent, while for compounds eluting close to the solvent peak, distortions occur with pure ACN. Water in the sample solvent favors electrostatic attraction for charged compounds, leading to increased retention time.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Xiaoya Zhang, Shuweng Hua, Quanshou Feng, Chuan-Fan Ding, Yongyao Wu, Yinghua Yan
Summary: Novel hydrophilic poly(N, N-methylenebisacrylamide/1,2-epoxy-5-hexene) coated magnetic nanospheres functionalized with 2-aminopurine (Fe3O4@poly(MBA/EH)@2AP) were successfully obtained using a simple and green method. The material showed high hydrophilicity and could capture glycopeptides and glycosylated exosomes within 1 min. It demonstrated great sensitivity, loading capability, selectivity, and repeatability, making it suitable for practical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Applied
Stephen Gitonga Giteru, M. Azam Ali, Indrawati Oey
Summary: Interest in using zein as an ingredient in food and bio-based materials has been increasing in the last decade. Understanding the interaction mechanisms of zein with other compounds is crucial for improving the structure-function relationship and expanding its applications in various industries. By exploring the assembly mechanisms and aggregation behavior of zein in composite macromolecular systems, specialized biomaterials can be developed for food, agricultural, biomedical, and cosmetic applications.
FOOD HYDROCOLLOIDS
(2021)
Article
Biochemical Research Methods
Xiaoyu Zhou, Hongyan Zhang, Li Wang, Ren'an Wu
Summary: This study developed a dual-functionalized metal-organic framework UIO-PBA&FDP for the efficient enrichment of glycopeptides in proteomics using the HILIC strategy. The UIO-PBA&FDP exhibited excellent hydrophilicity and successfully enriched 359 N-linked glycopeptides from human serum, achieving a high coverage of 104 glycoproteins.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
N. Yu Chikurova, A. O. Shemiakina, O. A. Shpigun, A. Chernobrovkina
Summary: A series of novel HILIC stationary phases containing both amides and hydroxyl groups were obtained on an aminated silica surface through multicomponent Ugi reaction. These stationary phases showed good separation efficiency and selectivity in chromatographic parameters and model mixtures.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Peizhen Li, Rongmao Lu, Jie Zhu, Qingqing Ke, Dingnan Wang, Yang Wang, Chen Zhang, Xinyue Lu, Miaolong Zhao, Zhang Wang, Lingling Pan, Meiling Zhang, Jun Ying, Shiyan Li
Summary: A sensitive and reliable ultra-performance hydrophilic interaction chromatography-tandem mass spectrometry method was developed to determine paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) in marine shellfish. The method involved the use of a HILIC-Z column for chromatographic separation and a porous carbon solid-phase extraction for sample clean-up. The calibration curves for PSTs and TTX were linear, with good sensitivity and low limits of detection and quantitation. The method was successfully applied to determine PSTs and TTX in three batches of Nassarius, showing excellent accuracy.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Biochemical Research Methods
Lidia Redon, Xavier Subirats, Marti Roses
Summary: The study used pycnometric and homologous series retention methods to investigate the volume and mean composition of water-rich layers partially adsorbed on the surface of HILIC column fillings with acetonitrile-water and methanol-water as eluents. The findings suggest that water is preferentially adsorbed on the surface of the HILIC bonded phase in hydroorganic eluents with high acetonitrile or methanol concentrations, forming several water-rich transition layers. In eluents with low acetonitrile or methanol concentrations, the amount of preferentially adsorbed water is minimal, and the retention behavior is similar to that of reversed-phase liquid chromatography.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Lana Abou Zeid, Albert Pell, Theo Tytus, Pascale Delangle, Carole Bresson
Summary: This study utilized HILIC-ESI-MS to separate multiphosphorylated cyclic peptides and their positional isomers, evaluating the selectivity of HILIC columns and the impact of mobile phase on separation selectivity. It explored the retention mechanism of different peptides, finding that the classic hydrophilic partitioning mechanism was followed exclusively on amide and diol columns. The research also revealed that the position of phosphorylated amino acids in the peptide backbone had an impact on retention, enabling the separation of positional isomers using HILIC.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Engineering, Environmental
Heejeong Ryu, Baikun Li, Sylvain De Guise, Jeffrey McCutcheon, Yu Lei
Summary: PFASs are persistent anthropogenic chemicals that are difficult to degrade in the environment. While current detection methods heavily rely on chromatographic techniques coupled with mass spectrometry, alternative methods such as optical and electrochemical techniques have been extensively explored for simple, accessible, inexpensive, rapid, and sensitive detection of PFASs. These alternative methods aim to overcome the limitations of expensive instrumentation, professional operators, complicated sample pretreatment, and considerable analysis time associated with chromatographic techniques.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemical Research Methods
Mark R. Taylor, Jane Kawakami, David V. McCalley
Summary: Sample injection in HILIC can cause issues when the injection solvent has higher elution strength than the mobile phase, resulting in asymmetric peak shapes and poor efficiency. Strategies to overcome this problem include pre-column dilution with a weak solvent and using alternative organic solvents in the injection solvent. Controlling the volume of injection solvents and using small sample volumes is recommended to reduce mismatch effects.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Qiong Jiang, Mingliang Zhang, Xusheng Wang, Yong Guo, Hongdeng Qiu, Shusheng Zhang
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2015)
Article
Biochemical Research Methods
Yong Guo, Rajan Shah
JOURNAL OF CHROMATOGRAPHY A
(2016)
Article
Multidisciplinary Sciences
Lanping Yang, Guoxiang Sun, Yong Guo, Zhifei Hou, Shuai Chen
Article
Chemistry, Analytical
Guoxiang Sun, Yu Wu, Zhongbo Liu, Yanfei Li, Yong Guo
ANALYTICAL METHODS
(2014)
Article
Biochemical Research Methods
Yong Guo, Sriram Srinivasan, Sheetal Gaiki, Ying Liu
Article
Biochemical Research Methods
Yong Guo, Sriram Srinivasan, Sheetal Gaiki
Article
Pharmacology & Pharmacy
Zhongbo Liu, Ying Peng, Bingjie Ma, Kashun Bi, Yingchun Liu, Guoxiang Sun, Gong Ping, Yong Guo
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2014)
Review
Biochemical Research Methods
Yong Guo, Sheetal Gaiki
JOURNAL OF CHROMATOGRAPHY A
(2011)
Article
Multidisciplinary Sciences
Zhifei Hou, Guoxiang Sun, Yong Guo
Article
Plant Sciences
Yujing Zhang, Fangliang Yang, Jing Zhang, Guoxiang Sun, Chao Wang, Yong Guo, Ran Wen, Wanyang Sun
Article
Physics, Atomic, Molecular & Chemical
Pengyi Zhao, Yong Guo, Howard D. Dewald, Hao Chen
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Biochemistry & Molecular Biology
Yong Guo, Dominik Baran
Summary: This study quantitatively determines the contributions of two major retention mechanisms, hydrophilic partitioning and surface adsorption, in HILIC to the overall retention of neutral compounds. The evaluation of the effect of acetonitrile on retention in HILIC is also carried out.
Article
Chemistry, Analytical
Yong Guo, Nidhi Bhalodia, Bassel Fattal
Article
Chemistry, Analytical
Yong Guo, Nidhi Bhalodia, Bassel Fattal, Ioannis Serris