Article
Chemistry, Analytical
Wei Wang, Anna Kaluza, Jan Nouta, Simone Nicolardi, Miroslawa Ferens-Sieczkowska, Manfred Wuhrer, Guinevere S. M. Lageveen-Kammeijer, Noortje de Haan
Summary: Altered total seminal plasma glycosylation has been associated with male infertility, but the exact role of PSA glycosylation in male fertility remains unclear. A novel high-throughput PSA glycopeptide workflow was developed for studying PSA glycosylation in seminal plasma, showing complex-type glycans with high levels of fucosylation and sialylation.
Article
Multidisciplinary Sciences
Cristina Di Carluccio, Rosa Ester Forgione, Marco Montefiori, Monica Civera, Sara Sattin, Giovanni Smaldone, K. Fukase, Y. Manabe, Paul R. Crocker, Antonio Molinaro, Roberta Marchetti, Alba Silipo
Summary: Siglecs are a group of cell-surface receptors belonging to the I-type lectin family, mostly expressed on innate immune cells, among which CD22 plays a key role in maintaining tolerance and preventing autoimmune responses. This study focused on the interaction of CD22 with sialoglycans, specifically exploring the role of N-glycolyl neuraminic acid (Neu5Gc) in this process. Findings suggested a comparable molecular recognition profile between human and murine forms of CD22, providing insights for innovative diagnostic and therapeutic methodologies in immune response modulation.
Article
Chemistry, Multidisciplinary
Yunlong Chen, Yuanjiao Yang, Qingqing Tan, Huipu Liu, Huangxian Ju
Summary: Sialylation plays a crucial role in tumor-related processes, and this study presents a strategy to suppress tumor growth by diverting intracellular sialylation using multifunctional mesoporous silica nanoparticles. The designed nanoparticles efficiently inhibit sialic acid expression on the tumor cell surface, leading to effective tumor suppression in mice. This approach holds great potential for exploring sialic acid-related biological processes and clinical cancer therapy.
Article
Multidisciplinary Sciences
Hyo Jeong Kim, Stephanie Schweiker, Katie Powell, Stephan Levonis
Summary: This paper outlines a simple method to detect and quantify sialic acids in cancer cells for evaluating sialyltransferase activity of potential therapeutic compounds. The proposed method is relatively simple, cost and time effective compared to pre-existing methods, and can successfully determine sialic acids in complex cancer cell matrix.
Article
Chemistry, Multidisciplinary
Naoto Ishida, Kazuki Kamada, Terumasa Omatsu, Kohji Maeda, Yumi Yoshida
Summary: This study demonstrates the uphill accumulation of a hydrophobic cation into the inner phase of a giant unilamellar vesicle through the concentration gradient of the counter anion. The theoretical simulation rationalizes the accumulation behavior and provides insights for the preparation of liposomal drugs.
Review
Chemistry, Multidisciplinary
Nico J. Overeem, Erhard van der Vries, Jurriaan Huskens
Summary: This review explores how influenza viruses navigate mucus and recognize host cells through multivalent interactions. The surface proteins of the influenza A virus facilitate specific binding to host cells, and the importance of these multivalent interactions in crossing mucus and entering host cells is discussed. Techniques to measure the avidity and receptor specificity of influenza viruses are also reviewed.
Article
Biochemistry & Molecular Biology
Nicolas Kint, Thomas Dubois, Patrick H. Viollier
Summary: Glycosylation of surface structures enhances the chemical and physical diversity of cells. The sialic acids pseudaminic acid (Pse) and legionaminic acid (Leg) play important roles as glycosyl donors in eubacteria and archaea. The protein sialyltransferase FlmG is responsible for O-glycosylating flagellins, the building blocks of flagellar filaments. By studying Caulobacter crescentus and Brevundimonas subvibrioides, we found that Pse and Leg biosynthesis pathways, as well as specific FlmG orthologs, contribute to flagellin glycosylation and motility. We developed a genetic glyco-profiling platform, identified a signature determinant of eubacterial and archaeal Leg biosynthesis, and validated it in a heterologous host. Furthermore, we discovered two modular determinants that control flagellin glycosyltransferase specificity.
Article
Agriculture, Multidisciplinary
Junhuan Yang, Jose T. Canovas-Marquez, Pengcheng Li, Shaoqi Li, Junchao Niu, Xiuwen Wang, Yusuf Nazir, Sergio Lopez-Garcia, Victoriano Garre, Yuanda Song
Summary: In this study, the deletion of two plasma membrane malate transporters in Mucor circinelloides WJ11 led to increased lipid content and altered expression of key genes involved in lipid biosynthesis, providing evidence of the association between plasma membrane malate transporters and lipid accumulation in oleaginous fungi.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Israel Alshanski, Ariel Shitrit, Yonatan Sukhran, Carlo Unverzagt, Mattan Hurevich, Shlomo Yitzchaik
Summary: Sensing enzymatic sialylation provides new tools for evaluating pathological events and pathogen invasion. The use of label-free biosensors can reduce the amount of substrate required, but their sensitivity to changes at the interface is high. This study demonstrates that the characteristics of the interface play a significant role in the biosensing of enzymatic sialylation, with only neutral mixed monolayers providing signals directly related to the process.
Article
Chemistry, Applied
Ke Wang, Ge Zhu, Ya-li Li, Shu-qi Chen, Arif Rashid, Xiao-tong Wang, Xin-yu Wu
Summary: Microwave irradiation can be used as a physiological regulator to alleviate chilling injury in postharvest fruit. The non-thermal effects of microwave treatment, particularly at 45.5 W for 7 min without inducing thermal effects, significantly inhibit internal browning caused by chilling injury and reduce total phenolic content. Furthermore, microwave treatment maintains membrane stability by inhibiting membrane lipid degradation and promoting sucrose accumulation.
Article
Biophysics
Abby Weston, Sorin-Cristian Vladescu, Tom Reddyhoff, Alex Griffiths, Thomas Crouzier, Matthew Fielden, James. A. Garnett, Guy. H. Carpenter
Summary: Mucus reduces friction by providing lubrication, and positively charged ions can influence the structure of mucin films and increase lubrication by drawing in water molecules. Sialic acids play an important role in lubrication through the sacrificial layer mechanism. The concentration of ions can affect mucin films and their lubrication, and sialic acids may be important for ion binding.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Immunology
Xuemei Hu, Yueyue Li, Qingyang Chen, Tingting Wang, Limei Ma, Wanping Zhang, Ruihong Yu, Jun Zhang, Jingyuan Wan, Chao Yu, Zhiyi Yuan
Summary: Accumulating evidence suggests that sialic acids play a crucial role in the development of atherosclerosis, but the specific effects and mechanisms have not been fully defined. This study demonstrates that sialic acids can promote the polarization of macrophages to the proinflammatory M1 phenotype, leading to the expression of proinflammatory cytokines. This proinflammatory effect is likely due to the inhibition of the LKB1-AMPK-Sirt3 signaling pathway, resulting in increased intracellular ROS levels and impaired autophagy-lysosome system, ultimately exacerbating atherosclerosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Asuka Shirakawa, Yoshiyuki Manabe, Roberta Marchetti, Kumpei Yano, Seiji Masui, Alba Silipo, Antonio Molinaro, Koichi Fukase
Summary: The fully sialylated tetraantennary N-glycan was synthesized for the first time using a specific strategy, and the synthesized compounds were used to study the recognition mechanism of neuraminidase.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Alexandra T. Linder, Michael Schmidt, Julia Hitschfel, Markus Abeln, Pascal Schneider, Rita Gerardy-Schahn, Anja K. Muenster-Kuehnel, Lars Nitschke
Summary: This study investigates the role of sialoglycans on B cells in cell migration, complement system control, and cellular signaling regulation. The results demonstrate that sialoglycans are crucial for the survival of B cells by counteracting multiple death-inducing pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Immunology
Taina Cavalcante, Mariana Medina Medeiros, Simon Ngao Mule, Giuseppe Palmisano, Beatriz Simonsen Stolf
Summary: Sialic acids (Sias) play important roles in cell communication, infection, and survival of pathogens on the cell surface. More than 20 pathogens can incorporate Sias from hosts to enhance survival inside the host, especially evident in Leishmania infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Food Science & Technology
Georg Sandner, Alice Koenig, Melanie Wallner, Julian Weghuber
Summary: In the field of (food) toxicology, there is a growing trend to replace animal trials with alternative methods to assess adverse health effects in humans. While advancements have been made in in vitro cell culture and in silico modeling, there is a lack of accurate predictions of metabolic actions. Alternative in vivo models are expected to bridge the gap between cell culture and animal trials.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Multidisciplinary Sciences
Fabian Hinterer, Magdalena C. Schneider, Simon Hubmer, Montserrat Lopez-Martinez, Philipp Zelger, Alexander Jesacher, Ronny Ramlau, Gerhard J. Schuetz
Summary: Single molecule localization microscopy (SMLM) has the potential to resolve structural details of biological samples at the nanometer scale. By performing SMLM under cryogenic temperature, higher localization precision can be achieved. However, it is important to consider the anisotropic emission characteristics of dipole emitters with fixed orientation to fully exploit the resolution. This study demonstrates the use of astigmatic imaging and dipole orientation information to extract the position of dipole emitters without bias and reach a precision of 1 nm.
Review
Chemistry, Analytical
Mara Heckmann, Gerald Klanert, Georg Sandner, Peter Lanzerstorfer, Manfred Auer, Julian Weghuber
Summary: This article reviews the methods used to quantify the translocation of GLUT4 to the plasma membrane, including microscopy-based techniques and biochemical and spectrometric approaches. These methods are of great importance in researching and identifying substances with insulinomimetic properties.
METHODS AND APPLICATIONS IN FLUORESCENCE
(2022)
Article
Cell Biology
Cindy Dirscherl, Sara Loechte, Zeynep Hein, Janine-Denise Kopicki, Antonia Regina Harders, Noemi Linden, Andreas Karner, Johannes Preiner, Julian Weghuber, Maria Garcia-Alai, Charlotte Uetrecht, Martin Zacharias, Jacob Piehler, Peter Lanzerstorfer, Sebastian Springer
Summary: MHC-I FHC dimers are the prevalent species at the plasma membrane, with a tendency to cluster into higher order oligomers. This clustering leads to a moderate decrease in diffusion coefficient.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Analytical
Tina Karimian, Roland Hager, Andreas Karner, Julian Weghuber, Peter Lanzerstorfer
Summary: This article introduces a simplified activation procedure for depositing biomolecule micropatterns on untreated solid substrates, allowing for direct microcontact printing and strong protein attachment via avidity binding. The specificity and suitability of this method have been demonstrated in proof-of-concept experiments, enhancing the accessibility of protein micropatterning for cell biological research.
Article
Biochemistry & Molecular Biology
Ingrid Hartl, Veronika Brumovska, Yasmin Striedner, Atena Yasari, Gerhard J. Schuetz, Eva Sevcsik, Irene Tiemann-Boege
Summary: This study utilized micropatterning to enrich FGFR3 in specific areas of the cell membrane, and quantified receptor activation by observing the recruitment of downstream signaling molecule GRB2 to FGFR3 micropatterns. The results showed that mutations in FGFR3 altered receptor activity, with increased activity in G380R and K650Q mutants upon ligand addition, while decreased activity in K650E mutant. These findings provide further insight into the functional effects of mutations to FGFR3.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Johann Wachlmayr, Laxmicharan Samineni, Thomas Barta, Armin Speletz, Chenhao Yao, Hyeonji Oh, Harekrushna Behera, Tingwei Ren, Manish Kumar, Andreas Horner
Summary: Understanding the determinants of permeability and selectivity in biological channels is important for gaining biophysical insights and developing biomimetic separation membranes. However, current research tends to focus on relative permeabilities and uses methods that result in inaccurate values. This hinders the comparison between channels and the development of high-performance channels.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Agnes Mistlberger-Reiner, Sonja Sterneder, Siegfried Reipert, Sara Wolske, Veronika Somoza
Summary: This study demonstrates the functional role of extracellular vesicles and particles (EVPs) in regulating gastric acid secretion. Pharmacological inhibition of EVPs reduces histamine-induced proton secretion, and isolated EVPs derived from HGT-1 cells show a dose-dependent modulatory effect on proton secretion. These findings suggest that EVPs could be a previously undiscovered mechanism in the regulation of gastric acid secretion.
Article
Biophysics
Clara Bodner, Dominik Kiesenhofer, Gerhard J. Schuetz, Mario Brameshuber
Summary: The interplay and communication between cells rely on protein-protein interactions and the oligomerization of proteins. The TOCCSL approach combines photobleaching and single-molecule microscopy to detect the interactions of fluorescently labeled membrane proteins even at high surface densities. Extensive Monte Carlo simulations were used to provide a theoretical framework for quantitative interpretation of TOCCSL measurements.
BIOPHYSICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Florian Weber, Markus Axmann, Andreas Horner, Bettina Schwarzinger, Julian Weghuber, Birgit Plochberger
Summary: This article focuses on the research of lipoprotein particles (LPs) in cardiovascular diseases, especially their transport functions and parameters such as class distribution, accumulation, site-specific delivery, cellular internalization, and escape. The aim is to load hydrophilic cargo into LPs, and insulin is used as an example for successful incorporation into high-density lipoprotein (HDL) particles. Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM) were used to study and verify the successful incorporation. Single-molecule-sensitive FM and confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).
Article
Physics, Applied
Fabian Hinterer, Magdalena C. Schneider, Simon Hubmer, Montserrat Lopez-Martinez, Ronny Ramlau, Gerhard J. Schuetz
Summary: Single molecule localization microscopy relies on precise quantification of the positions of single dye emitters in a sample. The number of detected photons from each molecule determines the precision, which can be improved by recording at cryogenic temperatures to reduce photobleaching. However, long illuminations can cause image blur due to inevitable jitter or drift, which degrades localization precision. This paper demonstrates a method to largely eliminate drift effects by parallel recording fiducial marker beads and fitting the drift trajectory, applicable to drift magnitudes of several hundred nanometers per frame, and assuming fixed dipole orientations during each illumination.
APPLIED PHYSICS LETTERS
(2023)
Article
Cell Biology
Neus Sanfeliu-Cerdan, Frederic Catala-Castro, Borja Mateos, Carla Garcia-Cabau, Maria Ribera, Iris Ruider, Montserrat Porta-de-la-Riva, Adria Canals-Calderon, Stefan Wieser, Xavier Salvatella, Michael Krieg
Summary: This study reveals that the material properties of biomolecular condensates can change with time, and the MEC-2/stomatin condensates undergo a rigidity phase transition from fluid-like to solid-like, facilitating transport and mechanotransduction. This finding suggests that the rigidity phase transition has a physiological role in force transmission in mechanosensitive neurons.
NATURE CELL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Johann Wachlmayr, Gotthold Flaschner, Kristyna Pluhackova, Walter Sandtner, Christine Siligan, Andreas Horner
Summary: Water permeation through narrow biological channels is crucial for all forms of life, but the energetics behind it are still unknown. In this study, accurate activation energy measurements of water permeation through Aquaporin-1 and determination of single channel permeability were used to estimate the entropic barrier of water permeation. The calculated value of Delta S=2.01+/-0.82 J/(mol center dot K) provides a link between the activation energy and the water conduction rate, which contributes to better understanding of various biological and artificial channels with different pore geometries.
COMMUNICATIONS CHEMISTRY
(2023)
Article
Polymer Science
Karolis Norinkevicius, Jeppe Madsen, Line Elmstrom Christiansen, Sebastian Meier, Andreas Horner, Radoslaw Gorecki, Torsten Hoybye Bak Regueira, Anders Egede Daugaard
Summary: The synthesis of block copolymers based on polyethylene glycol (PEG) and polycaprolactone (PCL) was reported. PEG-b-PCL diblock copolymers were prepared using enzymatic and tin-mediated ring-opening polymerization. Well-defined PEG-b-PCL-b-PEG triblock copolymers were synthesized through the coupling of PEG-b-PCL diblock copolymers. The aggregation behavior of diblock and triblock copolymers was studied using a direct hydration technique. The inclusion of amine-functional diblock copolymer favored cylindrical micelle formation.
Article
Food Science & Technology
Nicole Ollinger, Alexandra Malachova, Michael Sulyok, Lisa Schutz-Kapl, Nicole Wiesinger, Rudolf Krska, Julian Weghuber
Summary: In this study, three reliable methods were used to identify mould species in food, aiming to estimate the safety of apparently non-moldy slices of bread and reduce food waste. The results showed that it might be safe to consume bread slices from lower layers of bread stacks, even if mould growth is visible on the outer slices.
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Saurabh Saraswat, Archana Chugh
Summary: Cell penetrating peptides (CPP) are important tools for intracellular delivery of molecules. In this study, a novel marine-derived CPP from Engraulis japonicus, called Engraulisin, was reported. Engraulisin demonstrated successful cellular uptake and selective antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA), making it a potential candidate for drug delivery.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Niki Baccile, Vincent Chaleix, Ingo Hoffmann
Summary: This study measured the bending rigidity of self-assembled structures formed by a new biobased glucolipid bioamphiphile using neutron spin-echo (NSE) technology. The results showed that the bending rigidity of these structures is lower or higher compared to phospholipid membranes, providing a new theoretical basis for the application of these new molecular systems.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Alain Bolano Alvares, Pablo E. A. Rodriguez, Gerardo D. Fidelio
Summary: The behavior of amphiphilic molecules at the air/water interface was studied to evaluate the arrangement formed in a confined area. The surface properties of zwitterionic DPPC lipid and A beta(1-40) amyloid peptide in mixed films were investigated at different temperatures. DPPC undergoes a phase transition depending on temperature and lateral pressure, which allows for the study of its influence on amyloid structure formation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Lukasz Plachta, Marzena Mach, Magdalena Kowalska, Pawel Wydro
Summary: Resveratrol, a popular phytoalexin found in grapes and red wine, has beneficial effects on the cardiovascular system, exhibits antiviral, antibacterial, and antifungal properties, and may have therapeutic effects against cancer. This study investigates the interaction of resveratrol with model cell membranes, demonstrating changes in their physicochemical parameters and highlighting the role of cholesterol content in resveratrol incorporation. The findings suggest that the molecular mechanism of action of resveratrol may involve interactions with lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Dailin Li, Dingyuan Shi, Lei Wang
Summary: G protein-gated inwardly rectifying potassium (GIRK) channels play a significant role in regulating cell excitability. This study investigated the ion permeation mechanism in GIRK2 mutants using molecular dynamic simulations and QM/MM methods. The results revealed the importance of a multi-ion distribution for ion conduction.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick Allen, Adam C. Smith, Vernon Benedicto, Abbas Abdulhasan, Vasanthy Narayanaswami, Enrico Tapavicza
Summary: We explore the stability, size, and structure of nanodiscs formed by the N-terminal domain of apolipoprotein E3 and a variable number of 1,2-dimyristoyl-sn-glycero-3-phosphocholine molecules. Our simulations suggest that nanodiscs containing 240 to 420 DMPC molecules are stable. The antiparallel configuration shows more protein-protein interactions and ionic contacts, as well as greater stability and rigidity compared to the parallel configuration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick M. Keating, Nicholas P. Schifano, Xinrui Wei, Matthew Y. Kong, Jinwoo Lee
Summary: Lassa virus (LASV) infection is dependent on the fusion of its viral membrane with the host cell membrane, and our study reveals that this fusion process involves pH-dependent conformational changes in the transmembrane domain of LASV. These findings provide valuable insights into the fusion mechanism of LASV and can be utilized in the design of therapeutics to combat Lassa virus infections and prevent its potential spread.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Ashley L. Bennett, Kristen N. Cranford, Austin L. Bates, Christopher R. Sabatini, Hee-Seung Lee
Summary: This study employed molecular dynamics simulations to elucidate the binding, folding, and insertion details of TP10W in the cell membrane. The simulations revealed that TP10W can translocate across the lipid membrane as a monomer, and the charged amino acid side chains can move between lipid leaflets. Comparison with point mutated variants demonstrated significant conformational changes due to charge distribution.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Jessica H. van Wonderen, Jason C. Crack, Marcus J. Edwards, Thomas A. Clarke, Gerhard Saalbach, Carlo Martins, Julea N. Butt
Summary: Electrogenic bacteria transfer excess respiratory electrons to metal oxide particles and electrodes through the MtrCAB complex. The crystal structure of MtrCAB from S. baltica OS185 was resolved, and liquid chromatography mass spectrometry was used to determine the mass values of the three proteins in purified MtrCAB complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Review
Biochemistry & Molecular Biology
Yi-Ming Chen, Ching-Tai Lu, Chia-Wen Wang, Wolfgang B. Fischer
Summary: A series of dye ligands were ranked based on docking experiments, selecting the most suitable ligands for binding with different proteins. Additionally, a comparison study was conducted on a series of repurposing drugs and known antivirals.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
