Article
Multidisciplinary Sciences
Yoshiaki Kise, Go Kasuya, Hiroyuki H. Okamoto, Daichi Yamanouchi, Kan Kobayashi, Tsukasa Kusakizako, Tomohiro Nishizawa, Koichi Nakajo, Osamu Nureki
Summary: Mechanisms of modulation of Kv4 channel complexes by auxiliary subunits are central to the physiological function of channels in the brain and heart. The structures of Kv4.2-KChIP1 and Kv4.2-DPP6S complexes reveal that KChIP1 prevents N-type inactivation and stabilizes the S6 conformation, while DPP6S interacts with the S1 and S2 helices to accelerate voltage-dependent movement. KChIP1 and DPP6S do not directly interact with each other in the ternary complex, suggesting two distinct modes of modulation contribute to evoke A-type currents.
Article
Multidisciplinary Sciences
Danyang Zhang, Jake F. Watson, Peter M. Matthews, Ondrej Cais, Ingo H. Greger
Summary: This study reveals the structure and active states of AMPAR complexes, demonstrating that TARP-gamma 8 and CNIH2 affect the gating regulation of AMPARs by binding to specific regions of the receptor, thereby promoting the transition to the active-state conformation.
Article
Chemistry, Multidisciplinary
Ping Duan, Yaping Wang, Lichuan Chen, Kai Qu, Junyang Liu, Qian-Chong Zhang, Zhong-Ning Chen, Wenjing Hong
Summary: Investigating the correlations of electron transport between multiple channels shows promise for the design of molecule-scale circuits with logic operations. The modulation of electronegativity provides an effective method for controlling electron transport through multiple channels and exploring their interactions. Experimental and theoretical studies suggest that electronegativity has a more significant effect on electron transport in dual-channel structures.
Article
Cell Biology
Jiangtao Zhang, Shiqi Liu, Junping Fan, Rui Yan, Bo Huang, Feng Zhou, Tian Yuan, Jianke Gong, Zhu Huang, Daohua Jiang
Summary: In this study, cryoelectron microscopy was used to obtain structures of the Slo2.2 channel in closed, open, and inhibitor-bound form, providing insights into its cation regulation and inhibition. The closed state of Slo2.2 contains multiple K+ and Zn2+ binding sites in the cytoplasmic gating ring domain, which stabilize the closed conformation. The open state reveals Na+-sensitive sites that induce gating ring expansion and rotation upon Na+ binding, leading to inner gate opening. Additionally, a potent inhibitor blocks the pore by binding to a pocket formed by the pore helix and S6 helix. These findings establish a structural framework for studying Slo2.2 channel gating, Na+ sensation, and inhibition.
Article
Biology
Joseph L. Ransdell, Jonathan D. Moreno, Druv Bhagavan, Jonathan R. Silva, Jeanne M. Nerbonne
Summary: The resurgent component of the voltage-gated sodium current (I-NaR) is a depolarizing conductance that regulates the firing properties of various neuronal cell types. The mechanism behind the generation of I-NaR, mediated by voltage-gated sodium channels, remains unclear. This study provides evidence that resurgent Na+ influx occurs as a result of fast inactivating Nav channels transitioning into an open/conducting state on membrane hyperpolarization, and the decay of I-NaR reflects the slow accumulation of recovered/opened Nav channels into a second inactivated state. The amplitude of I-NaR can be modulated by factors such as the accessory Nav beta 4 channel subunit.
Article
Multidisciplinary Sciences
Tamer M. Gamal El-Din, Timothy Lantin, Christopher W. Tschumi, Barbara Juarez, Meagan Quinlan, Julia H. Hayano, Jin Li, Larry S. Zweifel, William A. Catterall
Summary: Autism spectrum disorder affects over 1% of children in the United States, resulting in social interaction deficits, repetitive behaviors, and communication disorders. Genetic analysis through genome sequencing has identified over 500 genes with mutations in ASD, suggesting that altered control of action potential generation by gating pore current through mutant K(v)7 channels may be a potential pathogenic mechanism in autism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Psychology, Experimental
Jeehye An, Janet H. Hsiao
Summary: Negative mood may increase eye movement patterns focusing on the eyes, but the impact of mood changes on eye movement strategy classification and recognition performance is limited.
Article
Multidisciplinary Sciences
David K. Jones
Summary: Cardiac hERG channels consist of hERG 1a and hERG 1b subunits, which drive cardiac action potential repolarization by regulating the movement of voltage sensor domains (VSDs). This study found that VSD relaxation delays pore closure during repolarization and that the hERG 1a PAS domain plays a role in regulating VSD movement. These findings provide the first recordings of hERG gating charge at physiological temperature and demonstrate the presence of VSD relaxation in hERG channels at physiological temperature.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Chih-Yi Cheng, Wei-Liang Pai, Yi-Hsun Chen, Naomi Tabudlong Paylaga, Pin-Yun Wu, Chun-Wei Chen, Chi-Te Liang, Fang-Cheng Chou, Raman Sankar, Michael S. Fuhrer, Shao-Yu Chen, Wei-Hua Wang
Summary: This study investigates the intersystem Coulomb interactions in IL-functionalized InSe field-effect transistors through displacement current measurements. The research uncovers a strong self-gating effect and reveals the IL-phase-dependent transport characteristics. Raman spectroscopy confirms the dominance of self-gating caused by the correlation between the intra- and intersystem Coulomb interactions. This study is significant for understanding the capacitive coupling at the InSe/IL interface.
Article
Materials Science, Multidisciplinary
Youngjun Park, Min-Kyu Kim, Jang-Sik Lee
Summary: Efficient neuromorphic devices require artificial synapses capable of linear and symmetric synaptic weight updates. To prevent ion dissipation, synaptic transistors are designed to operate by ion injection into the channel layer, with a threshold switch used as an access device. These design features enable stable and long-term synaptic weight modulation, improving data retention and recognition accuracy in artificial neural networks.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)
Article
Biochemistry & Molecular Biology
Andrew Bogard, Pangaea W. Finn, Aviana R. Smith, Ilinca M. Flacau, Rose Whiting, Daniel Fologea
Summary: The voltage regulation of lysenin channels in artificial lipid membranes leads to hysteresis, bistability, and memory. The presence of Cu2+ ions strengthens the hysteresis behavior and prevents the reopening of closed channels, reinstating the voltage gating and memory of lysenin channels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Mathematics
Brecht Verbeken, Marie-Anne Guerry
Summary: Discrete time Markov models and semi-Markov models are widely used in manpower planning, but semi-Markov models have more flexible sojourn time distributions to accommodate duration of stay effects. Hybrid semi-Markov models aim to reduce model complexity by only considering duration of stay effects for applicable transitions.
Article
Environmental Sciences
Elias Rodrigues da Cunha, Celso Augusto Guimaraes Santos, Richarde Marques da Silva, Eloi Panachuki, Paulo Tarso Sanches de Oliveira, Naelmo de Souza Oliveira, Karina dos Santos Falcao
Summary: The Cerrado biome in Brazil has experienced environmental problems such as soil loss and increased water turbidity due to changes in land use/cover. This study evaluates the impacts of soil loss and sediment delivery ratio over the past 30 years, simulates future soil erosion scenarios, and investigates a sediment delivery event in 2018. The results highlight the importance of assessing land use changes on soil erosion and sediment export, and the need for better management practices to reduce soil loss and maintain ecosystem services.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Thermodynamics
Mohammad Naghian, Khosrow Hosseini
Summary: This article introduces the application of meshless methods in numerical research, with a focus on the Collocation Discrete Least Squares Meshless (CDLSM) method and its use in numerical modeling of density current.
NUMERICAL HEAT TRANSFER PART A-APPLICATIONS
(2023)
Article
Biochemical Research Methods
Sevada Hovsepyan, Itsaso Olasagasti, Anne-Lise Giraud
Summary: Natural speech perception requires coordinating bottom-up and top-down information flows, and beta oscillations can achieve optimal results. This is important for speech processing, but may also apply to other cognitive processes.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zafir Buraei, Hye Kyung Lee, Keith S. Elmslie
Article
Multidisciplinary Sciences
Sulan Luo, Dongting Zhangsuna, Peta J. Harvey, Quentin Kaas, Yong Wu, Xiaopeng Zhu, Yuanyan Hu, Xiaodan Li, Victor I. Tsetlin, Sean Christensen, Haylie K. Romero, Melissa McIntyre, Cheryl Dowell, James C. Baxter, Keith S. Elmslie, David J. Craik, Michael McIntosh
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Neurosciences
Renuka Ramachandra, Keith S. Elmslie
Article
Multidisciplinary Sciences
Haylie K. Romero, Sean B. Christensen, Lorenzo Di Cesare Mannelli, Joanna Gajewiak, Renuka Ramachandra, Keith S. Elmslie, Douglas E. Vetter, Carla Ghelardini, Shawn P. Iadonato, Jose L. Mercado, Baldomera M. Olivera, J. Michael McIntosh
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Neurosciences
Tyler L. Marler, Andrew B. Wright, Kristina L. Elmslie, Ankeeta K. Heier, Ethan Remily, Jeong Sook Kim-Han, Renuka Ramachandra, Keith S. Elmslie
JOURNAL OF NEUROPHYSIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Renuka Ramachandra, Stephanie Y. McGrew, James C. Baxter, Jason R. Howard, Keith S. Elmslie
Article
Physiology
Zafir Buraei, Haoya Liang, Keith S. Elmslie
JOURNAL OF GENERAL PHYSIOLOGY
(2014)
Article
Neurosciences
Viktor Yarotskyy, Guofeng Gao, Blaise Z. Peterson, Keith S. Elmslie
JOURNAL OF NEUROPHYSIOLOGY
(2012)
Article
Neurosciences
Renuka Ramachandra, Bassil Hassan, Stephanie G. McGrew, James Dompor, Mohamed Farrag, Victor Ruiz-Velasco, Keith S. Elmslie
JOURNAL OF NEUROPHYSIOLOGY
(2013)
Article
Neurosciences
James C. Baxter, Renuka Ramachandra, Dustin R. Mayne, Keith S. Elmslie
JOURNAL OF NEUROPHYSIOLOGY
(2014)
Article
Multidisciplinary Sciences
Renuka Ramachandra, Stephanie McGrew, Keith Elmslie
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2013)
Article
Neurosciences
Andrew B. Wright, Khrystyna Yu Sukhanova, Keith S. Elmslie
Summary: The exercise pressor reflex (EPR) originates in skeletal muscle and is activated by signals during exercise to increase arterial blood pressure. Muscle ischemia can inappropriately activate the EPR in patients with peripheral vascular disease or heart failure. K(V)7 channels in muscle afferents are regulated by bradykinin (BK) and may play a role in EPR activation. Retigabine, a K(V)7 channel activator, could potentially help reduce the exacerbated EPR in patients with cardiovascular disease.
JOURNAL OF NEUROPHYSIOLOGY
(2021)
Article
Neurosciences
Khrystyna Yu Sukhanova, Ankeeta Koirala, Keith S. Elmslie
Summary: During exercise, substances released during muscle contraction activate G-protein-coupled receptors (GPCRs) to increase neuronal activity and generate the exercise pressor reflex (EPR). The enhancement of NaV1.9 channel activity may be a mechanism by which GPCR activation leads to the generation of EPR.
JOURNAL OF NEUROPHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Viktor Yarotskyy, Keith S. Elmslie
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2012)
Article
Neurosciences
Andrew B. Wright, Yohei Norimatsu, J. Michael McIntosh, Keith S. Elmslie
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Saurabh Saraswat, Archana Chugh
Summary: Cell penetrating peptides (CPP) are important tools for intracellular delivery of molecules. In this study, a novel marine-derived CPP from Engraulis japonicus, called Engraulisin, was reported. Engraulisin demonstrated successful cellular uptake and selective antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA), making it a potential candidate for drug delivery.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Niki Baccile, Vincent Chaleix, Ingo Hoffmann
Summary: This study measured the bending rigidity of self-assembled structures formed by a new biobased glucolipid bioamphiphile using neutron spin-echo (NSE) technology. The results showed that the bending rigidity of these structures is lower or higher compared to phospholipid membranes, providing a new theoretical basis for the application of these new molecular systems.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Alain Bolano Alvares, Pablo E. A. Rodriguez, Gerardo D. Fidelio
Summary: The behavior of amphiphilic molecules at the air/water interface was studied to evaluate the arrangement formed in a confined area. The surface properties of zwitterionic DPPC lipid and A beta(1-40) amyloid peptide in mixed films were investigated at different temperatures. DPPC undergoes a phase transition depending on temperature and lateral pressure, which allows for the study of its influence on amyloid structure formation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Lukasz Plachta, Marzena Mach, Magdalena Kowalska, Pawel Wydro
Summary: Resveratrol, a popular phytoalexin found in grapes and red wine, has beneficial effects on the cardiovascular system, exhibits antiviral, antibacterial, and antifungal properties, and may have therapeutic effects against cancer. This study investigates the interaction of resveratrol with model cell membranes, demonstrating changes in their physicochemical parameters and highlighting the role of cholesterol content in resveratrol incorporation. The findings suggest that the molecular mechanism of action of resveratrol may involve interactions with lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Dailin Li, Dingyuan Shi, Lei Wang
Summary: G protein-gated inwardly rectifying potassium (GIRK) channels play a significant role in regulating cell excitability. This study investigated the ion permeation mechanism in GIRK2 mutants using molecular dynamic simulations and QM/MM methods. The results revealed the importance of a multi-ion distribution for ion conduction.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick Allen, Adam C. Smith, Vernon Benedicto, Abbas Abdulhasan, Vasanthy Narayanaswami, Enrico Tapavicza
Summary: We explore the stability, size, and structure of nanodiscs formed by the N-terminal domain of apolipoprotein E3 and a variable number of 1,2-dimyristoyl-sn-glycero-3-phosphocholine molecules. Our simulations suggest that nanodiscs containing 240 to 420 DMPC molecules are stable. The antiparallel configuration shows more protein-protein interactions and ionic contacts, as well as greater stability and rigidity compared to the parallel configuration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick M. Keating, Nicholas P. Schifano, Xinrui Wei, Matthew Y. Kong, Jinwoo Lee
Summary: Lassa virus (LASV) infection is dependent on the fusion of its viral membrane with the host cell membrane, and our study reveals that this fusion process involves pH-dependent conformational changes in the transmembrane domain of LASV. These findings provide valuable insights into the fusion mechanism of LASV and can be utilized in the design of therapeutics to combat Lassa virus infections and prevent its potential spread.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Ashley L. Bennett, Kristen N. Cranford, Austin L. Bates, Christopher R. Sabatini, Hee-Seung Lee
Summary: This study employed molecular dynamics simulations to elucidate the binding, folding, and insertion details of TP10W in the cell membrane. The simulations revealed that TP10W can translocate across the lipid membrane as a monomer, and the charged amino acid side chains can move between lipid leaflets. Comparison with point mutated variants demonstrated significant conformational changes due to charge distribution.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Jessica H. van Wonderen, Jason C. Crack, Marcus J. Edwards, Thomas A. Clarke, Gerhard Saalbach, Carlo Martins, Julea N. Butt
Summary: Electrogenic bacteria transfer excess respiratory electrons to metal oxide particles and electrodes through the MtrCAB complex. The crystal structure of MtrCAB from S. baltica OS185 was resolved, and liquid chromatography mass spectrometry was used to determine the mass values of the three proteins in purified MtrCAB complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Review
Biochemistry & Molecular Biology
Yi-Ming Chen, Ching-Tai Lu, Chia-Wen Wang, Wolfgang B. Fischer
Summary: A series of dye ligands were ranked based on docking experiments, selecting the most suitable ligands for binding with different proteins. Additionally, a comparison study was conducted on a series of repurposing drugs and known antivirals.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)