Article
Biochemistry & Molecular Biology
Marc-Andre Delsuc, Kathrin Breuker, Maria A. van Agthoven
Summary: Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry method that correlates precursor and fragment ion signals by manipulating ion motions. Phase-corrected absorption mode 2D mass spectrometry improves signal-to-noise ratios and resolving power significantly, making data interpretation easier.
Article
Biochemistry & Molecular Biology
Taran Driver, Nikhil Bachhawat, Leszek J. Frasinski, Jonathan P. Marangos, Vitali Averbukh, Marina Edelson-Averbukh
Summary: The article introduces a technique to accurately identify chimera spectra through two-dimensional mass spectrometry. This technique, based on 2D-PC-MS, can diagnose cases where the standard assumption is not valid.
Article
Chemistry, Analytical
Jennifer Roemer, Alexander Stolz, Steffen Kiessig, Bernd Moritz, Christian Neususs
Summary: This study presents a comprehensive platform for full characterization of individual CE(SDS)-separated peaks by CE(SDS)-capillary zone electrophoresis-top-down-MS, enabling reliable and unambiguous online MS2 identification of unknown compounds of low-abundant individual CE(SDS) peaks.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Environmental Sciences
Laura Rohler, Pernilla Bohlin-Nizzetto, Pawel Rostkowski, Roland Kallenborn, Martin Schlabach
Summary: Long-term monitoring of regulated organic chemicals in ambient air provides valuable information about their environmental fate and trends, essential for evaluating regulatory effectiveness. New suspect and non-target screening analytical strategies are effective in identifying emerging compounds.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2021)
Article
Agriculture, Dairy & Animal Science
Marta Marynowska, Agnieszka Herosimczyk, Adam Lepcznski, Marcin Barszcz, Adrianna Konopka, Aleksandra Dunislawska, Malgorzata Ozgo
Summary: This study examines the effect of different prebiotic carbohydrates on the protein and gene changes in the aorta of healthy nursery pigs. The results suggest that inulin-type fructans have the potential to positively influence the structural and physiological alterations in the aorta, contributing to the maintenance of vascular homeostasis.
Article
Biochemical Research Methods
Juraj Piestansky, Michaela Matuskova, Ivana Cizmarova, Petra Majerova, Andrej Kovac, Peter Mikus
Summary: A two-dimensional capillary isotachophoresis-capillary zone electrophoresis method hyphenated with tandem mass spectrometry was developed and validated for ultrasensitive quantification of serotonin in real human urine samples. This method demonstrated high precision, accuracy, and recovery rates, making it suitable for routine clinical screening or targeted metabolomic studies of serotonin in urine samples. The advanced method showed promising potential for effective and reliable analysis of serotonin levels in urine samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Junhui Li, Lei Li, Wenqing Gao, Shoudong Shi, Jiancheng Yu, Keqi Tang
Summary: A high-performance FAIMS-IMS-MS platform was developed for exploring the conformational diversity of bradykinin. The FAIMS-IMS technique can resolve multiple conformers of (BK + H+) ions, and the separations in FAIMS and IMS dimensions are largely orthogonal, resulting in a higher overall resolving power.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Oliver Hoecker, Mike Knierman, Jens Meixner, Christian Neusuess
Summary: CE hyphenated to ESI-MS is a well-established technique for analyzing charged analytes, and a new nanoflow sheath liquid CE-MS interface has been developed in this study to improve analysis accuracy and robustness.
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Chunbao Du, Ting Du, Zixi Chang, Changji Yin, Yuan Cheng
Summary: This article summarizes the current work in the interface investigation of 2D biomaterials, including the desirable features of 2D materials and the molecular level interactions between 2D materials and biomaterials. The methods for investigating the interface characteristics of 2D biomaterials and the research progress on biomaterial-assisted exfoliation are discussed. Finally, a critical assessment of the newly developed 2D biomaterials in biomedical applications is presented.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Biochemical Research Methods
Andrea Hochegger, Sebastiano Panto, Nick Jones, Erich Leitner
Summary: In September 2022, the European Commission released a new regulation allowing the use of novel technologies to generate data for establishing regulatory processes on recycled plastic materials for food contact. This study compared different analytical approaches for screening commercially purchased post-consumer recycled polypropylene buckets. The results highlight the importance of detailed characterization and the advantages and disadvantages of using conventional gas chromatography and comprehensive two-dimensional gas chromatography with mass spectrometry.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Polymer Science
Steve R. Lustig, Jan W. Andzelm, Eric D. Wetzel
Summary: A comparative study of linear and two-dimensional polyaramids at high temperatures reveals that 2D polymers offer higher thermal resilience and structural stability, making them advantageous for high temperature applications.
Article
Chemistry, Analytical
Qinlei Liu, Sandra Martinez-Jarquin, Wenjie Ge, Renato Zenobi
Summary: Understanding diseases requires understanding metabolic heterogeneity in cells. This technical note introduces a 3D printing technology for manufacturing an ionization source for mass spectrometry-based single-cell analysis. The all in-one 3D-printed electrospray ionization source simplifies the process and improves reproducibility. It has been successfully used for high-throughput analysis of cancer cells and can be adjusted for different cell sizes. This 3D-printed ionization source opens new possibilities for single-cell analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Natividad Jurado-Campos, Maria Garcia-Nicolas, Marta Pastor-Belda, Tom Bussmann, Natalia Arroyo-Manzanares, Brigida Jimenez, Pilar Vinas, Lourdes Arce
Summary: This study investigated the potential of using HS-UV-IMS for the untargeted volatile fingerprinting of organic and conventional olives, achieving a success rate of 94.39%. For olive oil samples, HS-GC-IMS and CE-UV techniques were employed for analysis, with CE-UV showing higher classification accuracy (95.76%) and more stability over time.
Article
Medicine, Legal
Kinga Kadziola-Dlugolecka, Oliwia Fraczak, Beata Tkacz-Szczesna, Ilona Kijewska, Radoslaw Wilczek, Krzysztof Makowski, Piotr Komorowski, Robert Bachlinski, Anna Trynda, Bogdan Walkowiak
Summary: This publication presents analytical data for four psychoactive substances, including methods for their synthesis and confirmation such as ESI-MS, GC-EI-MS, GC-FID, HPLC, Raman spectroscopy, and H-1 NMR. It also discusses characteristic fragmentation patterns for cannabinoids and the development of HPLC methods for qualitative and quantitative determination of all synthesized compounds. Additionally, it utilizes RAMAN and GC-FID as analytical tools for compound identification in stability tests.
AUSTRALIAN JOURNAL OF FORENSIC SCIENCES
(2021)
Article
Engineering, Biomedical
Piotr Komorowski, Paulina Sokolowska, Malgorzata Siatkowska, Marcin Elgalal, Marcin Rosowski, Krzysztof Makowski, Lidia Lipinska, Martyna Leszczewicz, Andrzej Styczynski, Kasper Fogel, Bogdan Walkowiak
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2020)
Article
Biochemistry & Molecular Biology
Agnieszka M. Kolodziejczyk, Magdalena Kucinska, Aleksandra Jakubowska, Paulina Sokolowska, Marcin Rosowski, Beata Tkacz-Szczesna, Piotr Komorowski, Krzysztof Makowski, Bogdan Walkowiak
JOURNAL OF MOLECULAR RECOGNITION
(2020)
Review
Biochemistry & Molecular Biology
Kamila Bialkowska, Piotr Komorowski, Maria Bryszewska, Katarzyna Milowska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Analytical
Oliwia Fraczak, Kinga Kadziola-Dlugolecka, Ilona Kijewska, Radoslaw Wilczek, Beata Tkacz-Szczesna, Krzysztof Makowski, Piotr Komorowski, Robert Bachlinski, Anna Trynda, Bogdan Walkowiak
VIBRATIONAL SPECTROSCOPY
(2020)
Article
Chemistry, Physical
Piotr Komorowski, Malgorzata Siatkowska, Marta Kaminska, Witold Jakubowski, Marta Walczynska, Magdalena Walkowiak-Przybylo, Witold Szymanski, Katarzyna Piersa, Patryk Wielowski, Paulina Sokolowska, Kamila Bialkowska, Krzysztof Makowski, Marcin Elgalal, Agnieszka Kierzkowska, Lechoslaw Ciupik, Bogdan Walkowiak
Article
Materials Science, Multidisciplinary
Malgorzata Siatkowska, Paulina Sokolowska, Kamila Bialkowska, Aleksandra Zimon, Magdalena Grala, Marcin Rosowski, Kinga Kadziola-Dlugolecka, Piotr Komorowski, Krzysztof Makowski, Daniel Reda, Bogdan Walkowiak
Summary: Diamond and diamond-like materials possess great potential in biomedical applications, but the toxicity and biocompatibility of different forms of diamond particles, especially at nano and micro levels, need to be carefully studied and monitored to ensure safe use in specific biomedical applications.
DIAMOND AND RELATED MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Kamila Bialkowska, Katarzyna Milowska, Sylwia Michlewska, Paulina Sokolowska, Piotr Komorowski, Tania Lozano-Cruz, Rafael Gomez-Ramirez, Francisco Javier de la Mata, Maria Bryszewska
Summary: The study investigated the potential of carbosilane dendrimers as carriers for siRNA in gene therapy, focusing on CBD-1 and CBD-2. Both dendrimers were able to complex with siRNA and transport it into cells, with differences observed in their cytotoxicity profiles. This research highlights the importance of dendrimers in enhancing cellular uptake and efficiency of siRNA delivery for gene therapy applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Paulina Sokolowska, Malgorzata Siatkowska, Marta Jozwiak-Bebenista, Piotr Komorowski, Marta Koptas, Edward Kowalczyk, Anna Wiktorowska-Owczarek
Summary: Diclofenac, a nonsteroidal anti-inflammatory drug, has been evaluated for its effects on the unfolded protein response (UPR) pathways in endothelial cells. The study found that diclofenac can restore ER homeostasis by diminishing the UPR.
Article
Chemistry, Multidisciplinary
Krzysztof Andrzejewski, Marcin Elgalal, Piotr Komorowski, Jan Poszepczynski, Bozena Rokita, Marcin Domzalski
Summary: The study aimed to develop a Fast-Track-Protocol for creating 3D anatomical models to shape surgical plates for treating complex pelvic fractures. Through the low-budget approach using open-source software and 3D printers, a hospital-based multidisciplinary team successfully conducted pre-surgical planning and treated pelvic fractures with improved surgical techniques and shorter operative times.
APPLIED SCIENCES-BASEL
(2022)
Article
Cell Biology
Kamila Bialkowska, Piotr Komorowski, Rafael Gomez-Ramirez, Francisco Javier de la Mata, Maria Bryszewska, Katarzyna Milowska
Summary: Cationic dendrimers have been found to be effective carriers for delivering siRNA into cells and protecting nucleic acids. In 3D cell cultures, CBD-2 exhibits higher cytotoxicity compared to CBD-1, while the opposite is observed in 2D cultures. Attaching siRNA to a dendrimer molecule does not increase cellular toxicity. CBD-2 shows higher efficiency in transporting siRNAs compared to CBD-1.
Article
Pharmacology & Pharmacy
Marta Jozwiak-Bebenista, Paulina Sokolowska, Malgorzata Siatkowska, Cecilia Analia Panek, Piotr Komorowski, Edward Kowalczyk, Anna Wiktorowska-Owczarek
Summary: The research investigated whether UPR signaling pathway activation in astrocytes may serve as a novel target for antidepressant drugs, and found that S-ketamine may have therapeutic effects.
Article
Medicine, General & Internal
Krzysztof Andrzejewski, Marcin Domzalski, Piotr Komorowski, Jan Poszepczynski, Bozena Rokita, Marcin Elgalal
Summary: This study demonstrated the use of open-source medical image reconstruction software and low-cost 3D anatomical models in pre-surgical planning of RHA. The results showed that this method is effective in determining the center of rotation and positioning acetabular cups in RHA.
Article
Biotechnology & Applied Microbiology
Krzysztof Makowski, Martyna Leszczewicz, Natalia Broncel, Lidia Lipinska-Zubrycka, Adrian Glebski, Piotr Komorowski, Bogdan Walkowiak
Summary: The study isolated 40 bacterial strains with cellulolytic activity, most of which were Gram-positive rod-shaped bacteria capable of growth at a minimum of 50 degrees C. Several strains exhibited significant carboxymethyl cellulase (CMCase) activity and hydrolysis capacities, with some identified as Bacillus licheniformis and Paenibacillus lactis. These thermotolerant strains offer potential as a source of new thermostable cellulases for various applications in circular bioeconomy.
FOOD TECHNOLOGY AND BIOTECHNOLOGY
(2021)