期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1837, 期 9, 页码 1548-1552出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2013.11.020
关键词
Chloroplast; Gene expression; Photosystem degradation; Chlamydomonas
资金
- Swiss National Foundation [31003A_133089/1]
- ERC [281341]
- Swiss National Science Foundation (SNF) [31003A_133089] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [281341] Funding Source: European Research Council (ERC)
A repressible/inducible chloroplast gene expression system has been used to conditionally inhibit chloroplast protein synthesis in the unicellular alga Chlamydomonas reinhardtii. This system allows one to follow the fate of photosystem II and photosystem I and their antennae upon cessation of chloroplast translation. The main results are that the levels of the PSI core proteins decrease at a slower rate than those of PSII. Amongst the light-harvesting complexes, the decrease of CP26 proceeds at the same rate as for the PSII core proteins whereas it is significantly slower for CP29, and for the antenna complexes of PSI this rate is comprised between that of CP26 and CP29. In marked contrast, the components of trimeric LHCII the major PSII antenna, persist for several days upon inhibition of chloroplast translation. This system offers new possibilities for investigating the biosynthesis and turnover of individual photosynthetic complexes in the thylakoid membranes. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy. (C) 2013 Elsevier B.V. All rights reserved.
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