Article
Biochemistry & Molecular Biology
Jann-Louis Hau, Susann Kaltwasser, Valentin Muras, Marco S. Casutt, Georg Vohl, Bjoern Claussen, Wojtek Steffen, Alexander Leitner, Eckhard Bill, George E. Cutsail, Serena Debeer, Janet Vonck, Julia Steuber, Guenter Fritz
Summary: This study reveals that ion pumping in Na+-NQR is driven by large conformational changes coupling electron transfer to ion translocation. This mechanism is of significant importance for NADH oxidation in the human pathogen Vibrio cholerae.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Roman Rohac, Lydie Martin, Liang Liu, Debashis Basu, Lizhi Tao, R. David Britt, Thomas B. Rauchfuss, Yvain Nicolet
Summary: FeFe-hydrogenases use a unique H cluster to convert H-2 into protons and low-potential electrons. The [2Fe](H) center is where the reaction occurs and is built stepwise by maturating enzymes, with HydE performing complex modifications of complex-B to produce a precursor to the [2Fe](H) center.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Lei Zhang, Xueqi Wang, Jingxin Chen, Shaohu Sheng, Thomas R. Kleyman
Summary: Using crosslinking and voltage clamp techniques, we identified specific intersubunit contacts in ENaC that modulate channel activity. Crosslinking between specific residues in the palm and thumb domains of different subunits activated or inhibited ENaC activity. These findings suggest the presence of microswitches at specific subunit interfaces that play a role in ENaC gating and regulation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Keke Qu, Kangjing Chen, Hao Wang, Xueming Li, Zhucheng Chen
Summary: This study reports the cryo-electron microscopy structure of NuA4 bound to the nucleosome, shedding light on the assembly of NuA4 and its role in nucleosome recognition and transcription co-activation.
Article
Horticulture
Hao Bai, Jiangfei Chen, Tong Gao, Ziyi Tang, Hao Li, Siyu Gong, Yue Du, Youben Yu, Weidong Wang
Summary: A novel NHX gene named CsNHX6 was cloned from tea plant, and its overexpression enhanced the salt tolerance of yeast and Arabidopsis by increasing the Na+ storage capacity of cells. CsNHX6 was localized in various organelles including Golgi, TGN, PVC and vacuole, and this localization was enhanced by salt stress.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Yongqing Yang, Xiuli Han, Liang Ma, Yujiao Wu, Xiao Liu, Haiqi Fu, Guoyong Liu, Xiaoguang Lei, Yan Guo
Summary: Plant metabolites are dynamically modified and distributed in response to environmental changes. Maintaining ion homeostasis under salt stress requires coordinated activation of two types of central regulators: plasma membrane (PM) H+-ATPase and Na+/H+ antiporter. In this study, we identified phosphatidylinositol (PI) and phosphatidylinositol 4-phosphate (PI4P) as key molecules in regulating ion homeostasis under salt stress in Arabidopsis.
Article
Genetics & Heredity
Yue Yang, Leifeng Xu, Wenxiang Li, Yuwei Cao, Mengmeng Bi, Pengfei Wang, Rui Liang, Panpan Yang, Jun Ming
Summary: The LpSOS1 gene from Lilium pumilum was found to enhance salt tolerance in plants by regulating ion homeostasis, reducing Na+/K+ ratio, and enhancing antioxidant enzyme activity. This discovery has potential bioresource value in breeding salt-tolerant crops.
Article
Multidisciplinary Sciences
Xizi Chen, Xiaotong Yin, Jiabei Li, Zihan Wu, Yilun Qi, Xinxin Wang, Weida Liu, Yanhui Xu
Summary: This study investigates the mechanisms of TFIID and Mediator proteins in regulating transcription initiation and RNA polymerase activity, revealing their interactions and mutual regulation through structural analysis, as well as the phosphorylation process of Pol II CTD by CDK7.
Article
Biology
Piotr Kolata, Rouslan G. Efremov
Summary: The study reveals the structure of Escherichia coli respiratory complex I, showing dynamic connections within the peripheral and membrane domains. Unique structural features stabilize the peripheral domain assembly while the membrane domain structure exhibits novel dynamics, suggesting an uncoupled conformation of the complex. Based on the structural data, a new simplified hypothetical coupling mechanism is proposed for the molecular machine.
Article
Chemistry, Multidisciplinary
Hyunho Kim, Patricia Saura, Maximilian C. C. Poverlein, Ana P. Gamiz-Hernandez, Ville R. I. Kaila
Summary: Complex I is a redox-driven proton pump that powers oxidative phosphorylation. The mechanism of how redox reactions in Complex I trigger proton pumping remains unclear. In this study, we show that proton-coupled electron transfer reactions in Complex I induce long-range conformational changes and establish a proton pathway, which is responsible for proton pumping. Our findings provide insights into the catalytic control of protein conformational changes and ion transport across biological membranes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Ralf Steinhilper, Gabriele Hoeff, Johann Heider, Bonnie J. Murphy
Summary: The membrane-bound formate hydrogenlyase (FHL) complex is a prototypical hydrogen-producing enzyme that connects formate oxidation to proton reduction. It has attracted intense interest due to its efficient hydrogen production during fermentation, reversibility, and evolutionary link to respiratory complex I. Despite being studied for over a century, the atomic structure of FHL remained unknown. In this study, cryo-EM structures of FHL were determined, revealing the importance of conserved loops and a metal-binding site near the interface of FdhF and HycF subunits.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Fariha Ansari, Belem Yoval-Sanchez, Zoya Niatsetskaya, Sergey Sosunov, Anna Stepanova, Christian Garcia, Edward Owusu-Ansah, Vadim Ten, Ilka Wittig, Alexander Galkin
Summary: Accurate measurement of the content of mitochondrial complex I and alpha-ketoglutarate dehydrogenase complex is crucial for disease research and evaluation of treatment efficacy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Stella Vitt, Simone Prinz, Martin Eisinger, Ulrich Ermler, Wolfgang Buckel
Summary: The study investigates the structure and function of Rnf complex from Clostridium tetanomorphum using cryo-EM technique and biochemical data. The research reveals the electron transfer route and the role of sodium ion translocation in the redox process, and proposes the existence of a sodium ion channel.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Eike Laube, Jakob Meier-Credo, Julian D. Langer, Werner Kuehlbrandt
Summary: The structure of mitochondrial complex I from Chaetomium thermophilum has been determined, revealing two distinct conformations and suggesting a universal mechanism of coupling electron transport to proton pumping.
Article
Chemistry, Multidisciplinary
John J. Wright, Olivier Biner, Injae Chung, Nils Burger, Hannah R. Bridges, Judy Hirst
Summary: This study demonstrates, for the first time, the reverse electron transfer (RET) through complex I in a synthetic proteoliposome system, and provides a complete kinetic characterization of RET catalysis. The researchers also reveal new mechanisms and physiological features of the reversibility of complex I catalysis by studying the regulation in mammalian enzyme and evaluating RET in other species.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Computer Science, Artificial Intelligence
Manisha Vashisht, Brijesh Kumar
Summary: This paper presents a novel approach using 3D color-texture feature for traffic sign detection, demonstrating superior performance compared to other algorithms on Chinese TSRD and Mapillary datasets.
Article
Pediatrics
Abhinav Anand, Shalimar, Manisha Jana, Devasenathipathy Kandasamy, Brijesh Kumar, Gajendra Singh, Vandana Jain
Summary: The study identified controlled attenuation parameter (CAP) based cutoffs for diagnosing and grading hepatic steatosis in adolescents with overweight/obesity. CAP scores showed good discriminative ability in diagnosing fatty liver in adolescents with overweight or obesity, with moderate positive correlation with MRI-estimated hepatic fat. The optimal CAP cutoffs for identifying different levels of hepatic steatosis were determined using receiver operating characteristic (ROC) curves.
INDIAN JOURNAL OF PEDIATRICS
(2022)
Article
Chemistry, Physical
Sandeep Kumar Ojha, Brijesh Kumar
Summary: Research on devices and circuits based on organic materials is being conducted globally, with a focus on OLET devices. The performance of pentacene compared to tetracene in terms of drive current is significantly higher. Furthermore, the reliability of OLET devices is enhanced through External Quantum Efficiency and power analysis.
Article
Chemistry, Physical
Sandeep Kumar Ojha, Brijesh Kumar
Summary: This paper discusses a detailed comparison among SG-OLET, DG-OLET, and C-OLET, with DG-OLET showing significantly higher performance than SG-OLET, while C-OLET is approximately comparable. Additionally, QDs-based OLETs exhibit higher drive currents compared to pentacene OSC-based devices. Detailed discussions on Coulomb blockade and single electron tunneling phenomena are provided.
Article
Surgery
Manjunath Maruti Pol, Devender Singh, Brijesh Kumar, Surabhi Vyas, Gaurav Joshi, Tulasi Ram, V Sreenivas
Summary: Cephalic veins with a diameter of less than or equal to 2.5 mm are associated with a high failure rate. Preoperative balloon angioplasty can improve the patency rate of arteriovenous fistula (AVF) compared to hydrostatic technique. This study compared the efficacy of two surgical techniques (hydrostatic dilatation with balloon angioplasty (HD + BA) versus hydrostatic dilatation with malleable dilatation (HD + MD)) on the patency rates of AVF in patients with cephalic veins with a diameter of less than or equal to 2 mm.
INDIAN JOURNAL OF SURGERY
(2023)
Article
Astronomy & Astrophysics
Brajesh Kumar, Avinash Singh, D. K. Sahu, G. C. Anupama
Summary: We present the optical imaging and low-resolution spectroscopic monitoring results of the supernova SN 2017iro in the nearby galaxy NGC 5480. With its moderate luminosity and similar light-curve evolution to other supernovae, SN 2017iro provides valuable insights into the formation and evolution processes of supernovae. The analysis of line velocities and line features also reveals information about the explosion parameters and oxygen mass of SN 2017iro.
ASTROPHYSICAL JOURNAL
(2022)
Article
Astronomy & Astrophysics
Rishabh Singh Teja, Avinash Singh, D. K. Sahu, G. C. Anupama, Brajesh Kumar, A. J. Nayana
Summary: We present spectroscopic and photometric observations of Type IIP supernova SN 2020jfo and estimate the synthesized mass of important elements in the explosion. Using hydrodynamical modeling, we determine the ejecta mass and progenitor stellar mass, and identify a secondary radiation source in the early phase. The study also reveals the interaction with circumstellar material due to enhanced mass loss prior to the supernova explosion.
ASTROPHYSICAL JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Neha Mishra, Bramha P. Pandey, Brijesh Kumar, Santosh Kumar
Summary: This article showcases the electronic properties of Mn-doped MoSe2 monolayer and nitrous oxide adsorbed Mn-doped MoSe2 monolayer using first principle calculations. The doped systems exhibit enhanced adsorption energy and magnetic moment compared to the pristine MoSe2 monolayer, showing stability and favorable in-plane magnetization. Band structure calculations reveal a modulated bandgap and changed conductivity, indicating robust ferromagnetism in both doped and adsorbed Mn-MoSe2 ML.
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2022)
Article
Geosciences, Multidisciplinary
Pawan Govil, Abhijit Mazumder, Shailesh Agrawal, Syed Azharuddin, Ravi Mishra, Hidayatullah Khan, Brijesh Kumar, Divya Verma
Summary: The study reveals changes in the southwest monsoon intensity and humidity levels in the western Bay of Bengal over the past 6,000 years, associated with the Roman and Medieval Warm Periods. The correlation between planktonic foraminifera relative abundance and sand percentage suggests a decline in productivity possibly due to terrigenous dilution.
JOURNAL OF ASIAN EARTH SCIENCES
(2022)
Article
Chemistry, Applied
Vijaya Shukla, Vikas Bajpai, Pratibha Singh, Prashant Rai, Nilesh Khandelwal, Anil N. Gaikwad, Bikarma Singh, Brijesh Kumar
Summary: This study confirmed the traditional use of Chamaecostus cuspidatus and Cheilocostus speciosus in treating diabetes and reducing lipid levels through qualitative and quantitative analysis as well as in-vitro experiments. The content of bioactive compounds varied significantly among different organs. The anti-adipogenic potential of specific parts of these plants was also observed.
NATURAL PRODUCT RESEARCH
(2023)
Article
Computer Science, Information Systems
Puspraj Singh Chauhan, Sandeep Kumar, Vipin Kumar Upaddhyay, Sanjay Kumar Soni, Rajan Mishra, Brijesh Kumar
Summary: In this work, we presented the asymptotic performance analysis for double shadowed kappa-mu fading channel. We studied the system performance in terms of various parameters and validated the asymptotic slope of the results using coding gain and diversity gain analysis.
Article
Reproductive Biology
Acharya Balkrishna, Pradeep Nain, Monali Joshi, Brijesh Kumar, Anurag Varshney
Summary: This study investigates the effects of Shivlingi seed oil on reproductive fecundity and shows its potential for improving impaired fertility in a zebrafish model. The research provides scientific evidence for the use of Shivlingi seeds in Ayurvedic treatment for infertility and suggests further investigations into its effects on human reproductive health.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Biochemical Research Methods
Niloofar Nayeri, Ping Li, Kamil Gorecki, Karin Lindkvist-Petersson, Pontus Gourdon
Summary: Transition metals like copper and zinc are essential for most organisms but can also be toxic at elevated levels. The Copper TRansporter protein family (CTRs) is responsible for maintaining appropriate levels of copper and has potential applications in cancer drug delivery and antifungal therapies. However, the structure, function, and regulation of CTRs are still not well understood, limiting their use in applied sciences. In this study, we report procedures to isolate and characterize CTR members using Saccharomyces cerevisiae as a production host, providing valuable insights for further research and drug discovery efforts.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Article
Agriculture, Dairy & Animal Science
Athidi Lokavya, Brijesh Kumar, Pradeep Kumar, Eliezer Lyngdoh, Kalpendra Kohli, Hitesh, Ganeshan, Jisna, Arun, Mukthar Ahmed Bhat, Harendra Kumar
Summary: This article reports a rare case of twin mummified fetus in a buffalo. Through rectal examination and ultrasound technology, abnormal structures in the uterus and active ovaries were observed. The fetuses were successfully induced and delivered through the use of drugs and manual intervention.
Article
Surgery
Anirudh Shukla, Brijesh Kumar
Summary: The eyeball and the orbit are closely related to the ENT region, and ophthalmological manifestations are common in the practice of Otorhinolaryngology. This study investigated the ophthalmological complications in patients with ENT diseases and their correlation with orbital extension. It highlighted the importance of a thorough understanding of orbital anatomy for surgeons dealing with this complex region.
INDIAN JOURNAL OF OTOLARYNGOLOGY AND HEAD & NECK SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)