Article
Plant Sciences
Jochen M. Buck, Peter G. Kroth, Bernard Lepetit
Summary: Photosynthetic organisms have evolved various photoprotective mechanisms to prevent high light-induced photodamage, among which energy-dependent fluorescence quenching (qE) serves as a rapid way to dissipate excess absorbed energy. Diatoms, as important primary producers, utilize Lhcx proteins and the xanthophyll cycle pigment diatoxanthin to provide qE. By studying the mechanisms of qE in diatoms, it was found that specific amino acids and peptide motifs play crucial roles in conferring qE, with a tryptophan residue identified as having a major influence on qE establishment. This structural explanation sheds light on the intimate link between Lhcx proteins and diatoxanthin in providing qE in diatoms.
Article
Plant Sciences
Norma Valeria Ahumada-Fierro, Ernesto Garcia-Mendoza, Jose Miguel Sandoval-Gil, Christine Johanna Band-Schmidt
Summary: The study found that the toxic potential of Chattonella is associated with high ROS production, possibly due to a lack of effective photoprotection mechanisms. The three strains of Chattonella exhibited different NPQ and XC characteristics under light conditions, with ROS production related to XC-related thermal dissipation.
JOURNAL OF PHYCOLOGY
(2021)
Article
Environmental Sciences
Guo-Ning Nan, Xiao-Qun Zhou, Xiu-Mei Zhang, Quan-Sheng Zhang, Zi-Min Hu, Rui-Ping Huang, Di Zhang
Summary: This study demonstrates that xanthophyll cycle-induced non-photochemical quenching (NPQ) can effectively protect Sargassum thunbergii from light fluctuations in the intertidal zone.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Guenter G. Lehretz, Anja Schneider, Dario Leister, Uwe Sonnewald
Summary: Expression of VPZ genes in potato plants accelerates the induction and relaxation of photoprotection under various light conditions but does not improve photosynthetic rate or plant growth. Instead, tuber yield decreases under fluctuating light conditions. The negative effect may be due to reduced radiation use efficiency caused by strong NPQ induction under high light conditions.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Dandan Lu, Yi Zhang, Aihong Zhang, Congming Lu
Summary: This review summarizes the light-signaling pathways involved in photoprotection mechanisms and discusses key questions in these processes, emphasizing the importance of research on light-signaling mechanisms in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Richard P. Beckett, Farida Minibayeva, Kwanele W. G. Mkhize
Summary: The main difference between sun and shade lichens lies in the rate at which NPQ relaxes in the dark, rather than the speed at which photosynthesis starts upon illumination. In microhabitats where light levels are rapidly changing, rapid relaxation of NPQ may enable lichens' photobionts to use available light most efficiently.
Article
Biochemistry & Molecular Biology
Artur Nosalewicz, Karolina Okon, Maria Skorupka
Summary: This study evaluates the effects of non-photochemical quenching (NPQ) components on the response of Arabidopsis thaliana to fluctuating light and water availability. It found that water deficit affects the dynamics of NPQ induced by fluctuating light and reduces plant capability to cope with it.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Nqobile Truelove Ndhlovu, Knut Asbjorn Solhaug, Farida Minibayeva, Richard Peter Beckett
Summary: Lichens have a problem of excess energy causing the formation of harmful reactive oxygen species. Fungi in the lichens can reduce reactive oxygen species formation by synthesizing melanins, while photobionts can dissipate excess energy through non-photochemical quenching. The use of fluorimetry techniques to measure non-photochemical quenching has limitations, but a dissection technique can accurately evaluate it. The level of non-photochemical quenching in melanised thalli may vary depending on the light history of the microhabitat and presence of other tolerance mechanisms.
Article
Plant Sciences
Renata Welc, Rafal Luchowski, Dariusz Kluczyk, Monika Zubik-Duda, Wojciech Grudzinski, Magdalena Maksim, Emilia Reszczynska, Karol Sowinski, Radoslaw Mazur, Artur Nosalewicz, Wieslaw I. Gruszecki
Summary: The research conducted with Arabidopsis thaliana revealed the synergistic effect of zeaxanthin and PsbS, showing that PsbS interferes with the formation of densely packed aggregates of thylakoid membrane proteins, thus affecting the incorporation and photophysical properties of xanthophyll cycle pigments. The study highlighted the importance of zeaxanthin in enhancing excitation quenching to protect the system against photo-damage, while violaxanthin led to minimized dissipation of excitation energy within supramolecular structures.
Article
Biochemistry & Molecular Biology
Setareh Jamali Jaghdani, Peter Jahns, Merle Traenkner
Summary: This study investigated the impact of varying magnesium concentrations on barley photosynthetic efficiency and photoprotective responses, revealing that CO2 assimilation is more sensitive to reduction of tissue magnesium concentrations, while supply with lower magnesium concentrations induced photo oxidative stress.
Article
Multidisciplinary Sciences
Yu Bai, Tianjun Cao, Oliver Dautermann, Paul Buschbeck, Michael B. Cantrell, Yinjuan Chen, Christopher D. Lein, Xiaohuo Shi, Maxwell A. Ware, Fenghua Yang, Huan Zhang, Lihan Zhang, Graham Peers, Xiaobo Li, Martin Lohr
Summary: Fucoxanthin is a major light-harvesting pigment in ecologically important algae. The biosynthetic pathway of fucoxanthin has been identified by studying knockout mutants of the diatom Phaeodactylum tricornutum. The pathway is more complex than expected and involves the metabolism of diadinoxanthin as a central regulatory hub. The study also reveals the evolution of genes for xanthophyll cycle enzymes and the alternative pathway in brown algae.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Reimund Goss, Daniela Volke, Lina Emilia Werner, Ronja Kunz, Marcel Kansy, Ralf Hoffmann, Christian Wilhelm
Summary: In this study, fucoxanthin chlorophyll protein (FCP) complexes of the centric diatom Thalassiosira pseudonana were prepared using low concentrations of a mild detergent and sucrose gradient ultracentrifugation. Two main FCP fractions were observed in the sucrose gradients, each with different protein, lipid, and pigment composition. Mass spectrometry analysis revealed the presence of the xanthophyll cycle enzyme DDE in both FCP fractions, with higher enrichment in the fraction enriched in FCP-A. In-vitro de-epoxidation assays showed a more pronounced conversion of DD to Dt in the fraction enriched in FCP-A compared to FCP-B.
Article
Biodiversity Conservation
Rosina Magana Ugarte, Maria Pilar Gomez-Serranillos, Adrian Escudero, Rosario G. Gavilan
Summary: Despite the high levels of UVB radiation, high temperatures, and drought stress in Mediterranean high-mountain plants, efficient photoprotective strategies are understudied. Different plant species exhibit significant differences in the functioning of the xanthophyll cycle, with some using it as a continuous photoprotective strategy and others using it only under adverse conditions. The involvement of the lutein cycle is considered crucial for sustaining photoprotection in these plants, enhancing their photosystems' optimal functioning.
Editorial Material
Plant Sciences
Juan B. Arellano
Summary: This study provides evidence for the importance of energy quenching in photosystem I (PSI) during prolonged drought stress in Jatropha curcas. It suggests that non-photochemical quenching (NPQ) in PSI is essential for minimizing PSI inactivation and allowing plant recovery after re-watering.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Sara Bernardo, Lia-Tania Dinis, Ana Luzio, Nelson Machado, Vicente Vives-Peris, Maria F. Lopez-Climent, Aurelio Gomez-Cadenas, Lorenzo Zacarias, Maria Jesus Rodrigo, Aureliano C. Malheiro, Carlos Correia, Jose Moutinho-Pereira
Summary: The study shows that kaolin application can enhance grapevine photoprotection, manage chlorophyll and carotenoid content, and limit down-regulation of both photochemistry and photoinhibition processes under high summer stress conditions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Limnology
Dany Croteau, Sebastien Guerin, Flavienne Bruyant, Joannie Ferland, Douglas A. Campbell, Marcel Babin, Johann Lavaud
Summary: By studying the photoacclimation plasticity of five Arctic diatom species across different light environments, it was found that their nonphotochemical quenching and xanthophyll cycle responses in darkness varied depending on their light niche occupancy. These traits are believed to be rooted in diverging photoadaption strategies that promote Arctic diatom success in their respective light niches.
LIMNOLOGY AND OCEANOGRAPHY
(2021)
Article
Environmental Sciences
Guo-Ying Du, Xue-Feng Zhong, Christine Dupuy, Shuai Che, Johann Lavaud
Summary: The herbicide diuron affects photosynthesis and vertical migration of intertidal microphytobenthos (MPB) assemblages. High concentrations of diuron significantly decrease maximum relative electron transport rate, maximal PS II quantum yield and non-photochemical quenching, while middle concentrations show recovery of these parameters after a period of time. Concentration-dependent parameters such as rETRmax, Fv/Fm and NPQ are more sensitive in assessing diuron toxicity.
MARINE POLLUTION BULLETIN
(2021)
Article
Plant Sciences
Jochen M. Buck, Peter G. Kroth, Bernard Lepetit
Summary: Photosynthetic organisms have evolved various photoprotective mechanisms to prevent high light-induced photodamage, among which energy-dependent fluorescence quenching (qE) serves as a rapid way to dissipate excess absorbed energy. Diatoms, as important primary producers, utilize Lhcx proteins and the xanthophyll cycle pigment diatoxanthin to provide qE. By studying the mechanisms of qE in diatoms, it was found that specific amino acids and peptide motifs play crucial roles in conferring qE, with a tryptophan residue identified as having a major influence on qE establishment. This structural explanation sheds light on the intimate link between Lhcx proteins and diatoxanthin in providing qE in diatoms.
Article
Plant Sciences
Anne Pajot, Johann Lavaud, Gregory Carrier, Matthieu Garnier, Bruno Saint-Jean, Noemie Rabilloud, Caroline Baroukh, Jean-Baptiste Berard, Olivier Bernard, Luc Marchal, Elodie Nicolau
Summary: Differences in lhc classification in Chromista were observed. A classification of the lhcf family with specific groups for haptophytes, diatoms, and seaweeds was proposed. Similarity analysis was performed to identify and characterize Fucoxanthin and Chlorophyll a/c-binding Protein (FCP) in the haptophyte microalgae Tisochrysis lutea. The study revealed dynamic changes in lhc gene expression with nitrogen depletion and the exclusive nighttime expression of the lhcx2 gene in T. lutea, suggesting its role in protecting cells from light exposure after prolonged darkness.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Dany Croteau, Thomas Lacour, Nicolas Schiffrine, Philippe-Israel Morin, Marie-Helene Forget, Flavienne Bruyant, Joannie Ferland, Augustin Lafond, Douglas A. Campbell, Jean-Eric Tremblay, Marcel Babin, Johann Lavaud
Summary: The diatom communities in the Arctic Ocean exhibit different adaptation strategies based on changes in light environment, driving seasonal taxonomic succession. These adaptation strategies are aligned with changes in light intensity, leading to the dominance of different diatom species in different habitats throughout the seasons.
JOURNAL OF ECOLOGY
(2022)
Article
Plant Sciences
Guilan Yu, Kensuke Nakajima, Ansgar Gruber, Carolina Rio Bartulos, Alexander F. Schober, Bernard Lepetit, Elizabeth Yohannes, Yusuke Matsuda, Peter G. Kroth
Summary: The presence of biochemical CO2 concentrating mechanisms in diatoms, particularly involving the mitochondrial PEPC2, suggests the common occurrence of these mechanisms in diatoms.
Editorial Material
Plant Sciences
Thomas Roach, Eunchul Kim, Lijin Tian, Bernard Lepetit
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jochen M. Buck, Marie Wunsch, Alexander F. Schober, Peter G. Kroth, Bernard Lepetit
Summary: Iron is a crucial factor in the growth and photoprotection mechanism of diatoms. We found that iron limitation leads to upregulation of Lhcx2 and enhances qE effect. In addition to changes in Lhcx2, iron limitation also causes other adaptive changes in diatoms.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Medicinal
Sebastien Guerin, Laura Raguenes, Dany Croteau, Marcel Babin, Johann Lavaud
Summary: This study investigated the potential of the polar diatom Fragilariopsis cylindrus in producing fucoxanthin (Fx) and the inter-convertible couple diadinoxanthin-diatoxanthin (Ddx+Dtx) by manipulating the growth light climate and temperature. The results showed that F. cylindrus is well-suited for Fx and Ddx+Dtx production under conditions of low temperature and light intensity, reaching similar productivity levels as temperate counterparts.
Article
Marine & Freshwater Biology
Juan Du, Disney Izquierdo, Jonathan Naoum, Leanne Ohlund, Lekha Sleno, Beatrix E. Beisner, Johann Lavaud, Philippe Juneau
Summary: This study aimed to understand how different ecophysiological characteristics affect the response of Arctic and temperate microalgae to pesticides. It found that Arctic microalgae were more resistant to certain pesticides, while being more sensitive to others, which can be attributed to their cell size, pigment content, ROS content, and protective mechanisms.
AQUATIC TOXICOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Anne Pajot, Johann Lavaud, Gregory Carrier, Thomas Lacour, Luc Marchal, Elodie Nicolau
Summary: This study investigated the photoprotection mechanisms in two clonal strains of T. lutea with different basal pigment compositions. It was found that the two strains had different strategies for dissipating excess light energy and synthesizing photoprotective pigments. The expression of specific genes involved in the xanthophyll pigment cycles was also correlated with these diverging phenotypes.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Environmental Sciences
Juan Du, Disney Izquierdo, Hai-Feng Xu, Beatrix Beisner, Johann Lavaud, Leanne Ohlund, Lekha Sleno, Philippe Juneau
Summary: In aquatic ecosystems, microalgae are exposed to light fluctuations at different frequencies due to daily and seasonal changes. This study investigates the impacts of the herbicides atrazine and simazine on the physiological responses of Arctic and temperate microalgae. The findings suggest that light plays a crucial role in regulating herbicide toxicity in both Arctic and temperate microalgal strains.
ENVIRONMENTAL POLLUTION
(2023)
Article
Ecology
Norico Yamada, Bernard Lepetit, David G. Mann, Brittany N. Sprecher, Jochen M. Buck, Paavo Bergmann, Peter G. Kroth, John J. Bolton, Przemyslaw Dabek, Andrzej Witkowski, So-Yeon Kim, Rosa Trobajo
Summary: Dinotoms, belonging to the family Kryptoperidiniaceae, have diatom-derived endosymbionts and go through three evolutionary stages. D. capensis is able to use various diatom species as kleptoplastids and exhibits different photosynthetic capacities depending on the diatom species. The ability of D. capensis to ingest supplemental diatoms as kleptoplastids may be a flexible strategy to use these diatoms as emergency supplies.
Article
Multidisciplinary Sciences
T. Lacour, E. Robert, J. Lavaud
Summary: This study investigates the characteristics of non-photochemical quenching (NPQ) in Tisochrysis lutea under different light and nutrient conditions. The findings reveal a strong sustained NPQ (NPQs) under specific environmental conditions. Additionally, a strong relationship between the xanthophyll pigment diatoxanthin (Dt) and the maximum quantum yield of photochemistry (Fv/Fm) is highlighted.
SCIENTIFIC REPORTS
(2023)
Meeting Abstract
Plant Sciences
Anne Pajot, Johann Lavaud, Gregory Carrier, Matthieu Garnier, Luc Marchal, Bruno Saint-Jean, Elodie Nicolau
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)