Article
Soil Science
Zhen Yang, Tianran Sun, Sara Kleindienst, Daniel Straub, Ruben Kretzschmar, Largus T. Angenent, Andreas Kappler
Summary: Biochar can participate in biogeochemical electron transfer processes due to its electron-accepting and donating capabilities (i.e., geobattery) and electron conductivity (i.e., geoconductor). These two functions were separately demonstrated to play a role in biogeochemical iron cycling and methane formation. Little is known about the coupled effect of both electron transfer mechanisms, even though naturally occurring electron transfer through biochar is expected to simultaneously rely on both geobattery and geoconductor mechanisms.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Cell Biology
Sue Ellen Brand, Martha Scharlau, Lois Geren, Marissa Hendrix, Clayre Parson, Tyler Elmendorf, Earl Neel, Kaila Pianalto, Jennifer Silva-Nash, Bill Durham, Francis Millett
Summary: Cytochrome c (Cc) has undergone accelerated evolution from anthropoid primates to humans, with several amino acid changes occurring near the binding site of the Cc:CcO complex. These changes affect the dissociation constant and the role of phosphorylation in regulating mitochondrial electron transport and membrane potential.
Article
Chemistry, Multidisciplinary
Mohamed Elsamadony, Ahmed Elreedy, Alsayed Mostafa, Manabu Fujii, Johannes Gescher, Sepehr Shakeri Yekta, Anna Schnurer, Jean-Francois Gaillard, Deepak Pant
Summary: This article delves into the mechanisms of nanosized metal derivatives (NMDs) impacting the production of methane (CH4) and hydrogen (H-2) in anaerobic digestion (AD) and dark fermentation (DF) processes, exploring effects on extracellular electron transfer, biotic-biotic interactions such as direct interspecies electron transfer (DIET), and biotic-abiotic exchanges mediated by redox reactions with metals.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Agricultural Engineering
Zhenxin Wang, Cheng Sun, Yue Zhou, Qilin Yu, Xiaochen Xu, Zhiqiang Zhao, Yaobin Zhang
Summary: Adding Fe(OH)3 to Anammox systems can enhance the activity of ammonia oxidizing microorganisms and improve NH4+-N removal in the Anammox sludge system. Fe(OH)3 can also promote the polarization of functional groups of outer membrane cytochrome, benefiting extracellular electron transfer.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Yufen Wang, Jiaqi Hou, Haixiao Guo, Tingting Zhu, Yaobin Zhang, Yiwen Liu
Summary: This study proposes a method using ferroferric oxide technology to increase the production of medium chain fatty acids (MCFAs) in waste activated sludge. The results show that ferroferric oxide enhances the yield of MCFAs in the sludge and changes the distribution of key microorganisms, while also improving sludge dewatering and phosphorus removal.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Guosheng Chen, Linjing Tong, Siming Huang, Shuyao Huang, Fang Zhu, Gangfeng Ouyang
Summary: This study presents the design of an exogenous hydrogen-bonded organic framework to modulate the conformation of cytochrome c, enabling non-native bioactivity for the enzyme. By manipulating the flexible conformation of the enzyme, the researchers demonstrate the advantages of artificial hydrogen-bonded scaffolds in modulating enzyme activity.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Agnes Moe, Justin Di Trani, John L. Rubinstein, Peter Brzezinski
Summary: Energy conversion in aerobic organisms involves electron transfer from low-potential donors to dioxygen through the membrane-bound respiratory chain. The formation of supercomplex CIII2CIV1/2 facilitates electron transfer by two-dimensional diffusion of cyt. c, increasing QH(2):O-2 oxidoreductase activity and suggesting a regulatory role for supercomplex formation in the respiratory chain.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biophysics
Yue Wu, Qing Du, Yuxuan Wan, Qian Zhao, Nan Li, Xin Wang
Summary: This study demonstrates the possibility of reverse bioelectron transfer in Geobacter-dominated electroactive biofilms, achieving a high efficiency of dissimilatory nitrate reduction to ammonia (DNRA). The use of constant current is found to be more effective in maintaining Geobacter on the cathode, and the prevalence of DNRA at constant current is shown through gene transcription analysis. Metatranscriptomics reveals potential electron uptake mechanisms in the biofilms.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Inorganic & Nuclear
Srijan Narayan Chowdhury, Sachidulal Biswas, Saikat Das, Achintesh N. Biswas
Summary: A pentadentate Cu(ii) complex catalyses the four-electron reduction of dioxygen by decamethylferrocene in the presence of trifluoroacetic acid. The use of stronger Bronsted acids inhibits the catalytic oxygen reduction. It is found that the strength of protonic acids increases significantly in acetone compared to that in dimethylformamide. Detailed kinetic studies reveal the mechanism of the reaction.
DALTON TRANSACTIONS
(2023)
Article
Environmental Sciences
Lei Chen, Mingpeng Wang, Yujie Feng, Xiaoyu Xu, Xiaobo Luo, Zhaojie Zhang
Summary: Xanthomonas, a common plant pathogen found in biocontaminated bioreactors, was found to exhibit characteristics such as reduction of metal Fe (III) and decolorization of methyl orange in vitro. This microorganism was able to generate bioelectricity and had two extracellular electron transfer pathways, with the indirect transfer method being dominant and faster than direct transfer. The study highlights a novel anaerobic mechanism for Xanthomonas survival during wastewater treatment and suggests potential strategies for preventing its growth.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Xing Yan, Jie Bu, Xiong Chen, Ming-Jun Zhu
Summary: Microbial extracellular respiration, including Fe(III) and electrode respiration, was observed in Thermoanaerobacterium thermosaccharolyticum MJ2. Comparative genomic analysis revealed the intra/extracellular electron transfer mechanism of MJ2, with hydrogenase and cytochrome c playing crucial roles. Moreover, the addition of exogenous Cyt-c significantly increased the bioelectricity generation by MJ2.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biophysics
Ethan Howley, Rosa Krajmalnik-Brown, Cesar I. Torres
Summary: This study combines electrochemical and gene expression data to identify electron carriers in Geobacter sulfurreducens that may be induced by the use of three different electron transfer pathways. Cyclic voltammetry experiments on biofilms grown at different redox potentials quantitatively assess the relative use of these pathways. Transcriptomic analyses reveal complex transcriptomic responses across the electron transfer pathways. This method can be adapted to study electron transport in other electroactive organisms with complex metabolisms.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Jesus Cases Diaz, Monica Gimenez-Marques
Summary: Alternative synthetic procedures enable the formation of protein@HKUST-1 biocomposites, overcoming the challenge of mineralization in water for hydrolytically unstable MOFs.
CHEMICAL COMMUNICATIONS
(2023)
Article
Crystallography
Rita Chertkova, Tatyana Bryantseva, Nadezhda A. Brazhe, Kseniya S. Kudryashova, Victor V. Revin, Alexei N. Nekrasov, Alexander Yusipovich, Alexey R. Brazhe, Andrew B. Rubin, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Georgy Maksimov
Summary: The secondary structure of horse cytochrome c with mutations in the P(76)GTKMIFA(83) site of the Omega-loop, exhibiting reduced efficiency of electron transfer, was studied. CD spectroscopy and IR spectroscopy data showed changes in structural elements, with an increase in beta-structural elements and a decrease in non-structured elements. H-1-NMR spectra analysis revealed specific features in the secondary structure related to changes in the heme microenvironment. These changes likely contribute to the decreased efficiency of electron transfer in the studied cytochromes c.
Article
Biochemistry & Molecular Biology
Giulia Di Rocco, Antonio Ranieri, Marco Borsari, Marco Sola, Carlo Augusto Bortolotti, Gianantonio Battistuzzi
Summary: The Met80Ala variant of yeast cytochrome c shows promising biocatalytic and biosensing properties due to its stable electrocatalytic properties in mixed solvents. Organic solvents have minimal effects on the redox and structural properties of the variant.
Article
Biochemistry & Molecular Biology
Katherine E. Frato, Kelly A. Walsh, Sean J. Elliott
Article
Biochemistry & Molecular Biology
Stephanie J. Maiocco, Arthur J. Arcinas, Bradley J. Landgraf, Kyung-Hoon Lee, Squire J. Booker, Sean J. Elliott
Article
Chemistry, Multidisciplinary
Anthony J. Blaszczyk, Alexey Silakov, Bo Zhang, Stephanie J. Maiocco, Nicholas D. Lanz, Wendy L. Kelly, Sean J. Elliott, Carsten Krebs, Squire J. Booker
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Article
Biochemistry & Molecular Biology
Lindsey M. Walker, William M. Kincannon, Vahe Bandarian, Sean J. Elliott
Article
Biochemistry & Molecular Biology
Matthew W. Wolf, Kimberly Rizzolo, Sean J. Elliott, Nicolai Lehnert
Article
Biochemistry & Molecular Biology
Richard Ayikpoe, Thacien Ngendahimana, Michelle Langton, Sheila Bonitatibus, Lindsey M. Walker, Sandra S. Eaton, Gareth R. Eaton, Maria-Eirini Pandelia, Sean J. Elliott, John A. Latham
Article
Biochemistry & Molecular Biology
Joshua T. Atkinson, Ian J. Campbell, Emily E. Thomas, Sheila C. Bonitatibus, Sean J. Elliott, George N. Bennett, Jonathan J. Silberg
NATURE CHEMICAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Kimberly Rizzolo, Steven E. Cohen, Andrew C. Weitz, Madeline M. Lopez Munoz, Michael P. Hendrich, Catherine L. Drennan, Sean J. Elliott
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Lindsey M. Walker, Bin Li, Dimitri Niks, Russ Hille, Sean J. Elliott
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2019)
Article
Chemistry, Inorganic & Nuclear
Andrew C. Weitz, Saborni Biswas, Kim Rizzolo, Sean Elliott, Emile L. Bominaar, Michael P. Hendrich
INORGANIC CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Kimberly Rizzolo, Andrew C. Weitz, Steven E. Cohen, Catherine L. Drennan, Michael P. Hendrich, Sean J. Elliott
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Multidisciplinary
Wen Zhu, Lindsey M. Walker, Lizhi Tao, Anthony T. Iavarone, Xuetong Wei, R. David Britt, Sean J. Elliott, Judith P. Klinman
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Physical
Bin Li, Phillip Steindel, Narmien Haddad, Sean J. Elliott
Summary: Modulating the electron-transfer steps in redox reactions has been proven as an effective strategy to regulate enzyme catalysis. By systematically engineering the reduction potential of the electron donor ferredoxin, the study successfully maximized catalytic CO2 reduction.
Article
Biochemistry & Molecular Biology
Victor Sosa Alfaro, Julius Campecino, Matthew Tracy, Sean J. Elliott, Eric L. Hegg, Nicolai Lehnert
Summary: Cytochrome c nitrite reductases (CcNIR or NrfA) are crucial for conserving usable nitrogen in the soil for the global nitrogen cycle. The study on Geobacter lovleyi NrfA revealed that hemes 4 and 5 serve as electron storage units within the protein, while heme 3 plays a critical role as an electron gateway. Additionally, electrochemical experiments showed a range of reduction potentials for each heme, impacting the enzyme's catalytic activity.
Article
Chemistry, Multidisciplinary
Haoran Pang, Lindsey M. Walker, Alexey Silakov, Pan Zhang, Weitao Yang, Sean J. Elliott, Kenichi Yokoyama
Summary: The diversity of reactions catalyzed by radical S-adenosyl-L-methionine (SAM) enzymes is achieved in part through various mechanisms to quench radical intermediates. Among the SPASM-twitch family, MoaA serves as a representative member with unique cyclization of GTP involving an auxiliary 4Fe-4S cluster. The radical quenching step in MoaA involves a proton-coupled electron transfer mechanism, providing detailed insights into radical SAM enzyme catalysis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)