Article
Chemistry, Physical
Margareta R. A. Blomberg
Summary: Improvements in density functional theory methodology have made it a powerful tool in studying enzymatic reaction mechanisms, but concerns remain about the reliability of results for metalloenzymes. By using different DFT functionals and combining with experimental data, stable qualitative pictures of reaction mechanisms were obtained.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Multidisciplinary Sciences
Alex J. Flynn, Svetlana V. Antonyuk, Robert R. Eady, Stephen P. Muench, S. Samar Hasnain
Summary: The cryoEM structure of qNOR from Alcaligenes xylosoxidans provides insights into its crucial role in the denitrification pathway and sheds light on the mechanisms of electron, substrate, and proton transport.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Pokhraj Ghosh, Molly Stauffer, Valiallah Hosseininasab, Subrata Kundu, Jeffery A. Bertke, Thomas R. Cundari, Timothy H. Warren
Summary: Copper nitrite reductases convert nitrite ions to nitric oxide and nitrous oxide at high levels of nitrogen oxide flux. The assembly of nitric oxide at a single copper site to form copper cis-hyponitrites has been demonstrated in the presence of an external reductant. Furthermore, the removal of a fraction of the structure in the copper cis-hyponitrite complex can be achieved by adding specific reagents, resulting in the formation of a spectroscopically similar anion. These studies provide insights into the reductive coupling of nitrogen oxides at a single copper center and the utilization of the strong oxidizing power of nitrite.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biochemistry & Molecular Biology
Sergey A. Siletsky, Vitaliy B. Borisov
Summary: Terminal respiratory oxidases are efficient molecular machines that play crucial roles in energy transfer and cell survival. Different types of oxidases have diverse origins and functional significances, yet they all share the ability to avoid the formation of dangerous substances while reducing oxygen to water. Recent advancements in studying their structures and functions are crucial for understanding their roles in cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Margareta R. A. Blomberg
Summary: Cellular respiration involves electron transport via enzyme complexes to terminal Cytochrome c oxidase, where molecular oxygen is reduced to water, establishing a transmembrane electrochemical gradient using the free energy released. The redox-active tyrosine in CcOs plays a crucial role in energy conservation by allowing proton pumping in all reduction steps. The unprotonated tyrosine provides a mechanism for coupling the uptake of two protons per electron in every reduction step, ensuring secure proton pumping.
FRONTIERS IN CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Jinghua Chen, Peilu Xie, Yujia Huang, Haichun Gao
Summary: Nitrite and nitric oxide are critical nitrogen oxides in the nitrogen biogeochemical cycle, capable of interacting with heme-copper oxidases for enzymatic interconversion and inhibition. While they show similar biochemical properties, they are treated as distinct molecules by bacterial cells and have different effects on enzymes and sensing systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pedro J. Pacheco, Juan J. Cabrera, Andrea Jimenez-Leiva, Eulogio J. Bedmar, Socorro Mesa, German Tortosa, Maria J. Delgado
Summary: This study provides a comprehensive investigation of the role of copper (Cu) in denitrification of Bradyrhizobium diazoefficiens. It reveals that Cu influences the expression and protein levels of denitrification-related genes, and also demonstrates a post-transcriptional regulation. The findings of this study are important for reducing N2O emissions from agricultural soils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Tomas Rodriguez-Gil, Alejandro Torrado, Macarena Iniesta-Pallares, Consolacion alvarez, Vicente Mariscal, Fernando P. Molina-Heredia
Summary: Cytochrome c(M) was first described in 1994 and its sequence has been found in the genome of many cyanobacterial species. Recent research suggests that its 3D structure is similar to the C subunit of bacterial nitric oxide reductase, potentially locating it in the thylakoid membrane and giving it a new role.
APPLIED SCIENCES-BASEL
(2021)
Article
Microbiology
Sarah L. Schwartz, Lily Momper, Luiz Thiberio Rangel, Cara Magnabosco, Jan P. Amend, Gregory P. Fournier
Summary: Denitrification plays a central role in the global nitrogen cycle. The study reveals a greater diversity of denitrification enzyme arrangements than previously reported.
Article
Biochemistry & Molecular Biology
John Ukeri, Michael T. Wilson, Brandon J. Reeder
Summary: Cytoglobin has physiological roles that are not clearly understood, but may include regulation of nitric oxide, oxygen sensing, and protection against oxidative stress. Specific mutations can affect the nitric oxide binding and metabolism activities of cytoglobin, helping to understand its precise role under physiological and pathophysiological conditions.
Article
Biochemistry & Molecular Biology
Sheng-Qing Gao, Jia-Jun Shi, Shu-Hao Miao, Tao Li, Chao-Chao Gao, Yan-Ling Han, Jia-Yin Qiu, Yun-Song Zhuang, Meng-Liang Zhou
Summary: This study investigated the contribution of the interaction between endothelial NOX4 and DHFR to eNOS uncoupling after SAH. Inhibiting endothelial NOX4 and increasing endothelial DHFR levels alleviated eNOS uncoupling and improved neurological function after SAH.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Arnab Ghosh, Cynthia J. Koziol-White, William F. Jester, Serpil C. Erzurum, Kewal Asosingh, Reynold A. Panettieri, Dennis J. Stuehr
Summary: The study revealed that severe asthmatics have inherent defects in sGC in their airway smooth muscle, leading to impaired NO-sGC-cGMP signaling. This dysfunction is associated with changes in cell redox enzymes that impact sGC maturation and function.
Article
Plant Sciences
Aprajita Kumari, Vemula Chandra Kaladhar, Nidhi Yadav, Pooja Singh, Kishorekumar Reddy, Kapuganti Jagadis Gupta
Summary: The location of NO production in mitochondrial cytochrome c oxidase and its role in mitochondrial biogenesis were investigated in Arabidopsis seedlings under osmotic stress and recovery. Osmotic stress reduced growth and mitochondrial number while increasing NO production. Nitrite application stimulated NO production and mitochondrial number in a NO-deficient mutant. COX6b(-)3 and COA6-L subunits were induced during osmotic stress and mutants impaired in these subunits showed reduced NO production and mitochondrial biogenesis. The COX6b-3 and COA6-L subunits interacted with a VQ27 motif-containing protein in the presence of NO, and a vq27 mutant displayed impaired mitochondrial biogenesis.
PLANT CELL AND ENVIRONMENT
(2023)
Review
Plant Sciences
Lovely Mahawar, Kesava Priyan Ramasamy, Aparna Pandey, Sheo Mohan Prasad
Summary: Iron is a crucial micronutrient for plant growth and human nutrition. Understanding iron homeostasis in plants is essential for improving growth and development and addressing iron deficiency in low-income rural populations. This review focuses on the role of signaling molecules, particularly phytohormones and nitric oxide, and their coordination in iron homeostasis in agriculturally important crops. These signaling molecules play a vital role in up-regulating iron acquisition genes, facilitating iron translocation, and enhancing iron accessibility, making them important biofortification strategies for addressing hidden hunger.
PLANT GROWTH REGULATION
(2023)
Article
Medicine, Research & Experimental
Nolan T. Carew, Heidi M. Schmidt, Shuai Yuan, Joseph C. Galley, Robert Hall, Helene M. Altmann, Scott A. Hahn, Megan P. Miller, Katherine C. Wood, Bethann Gabris, Margaret C. Stapleton, Sean Hartwick, Marco Fazzari, Yijen L. Wu, Mohamed Trebak, Brett A. Kaufman, Charles F. McTiernan, Francisco J. Schopfer, Placido Navas, Patrick H. Thibodeau, Dennis M. McNamara, Guy Salama, Adam C. Straub
Summary: This study reveals the crucial role of cytochrome b5 reductase 3 (CYB5R3) in cardiomyocyte redox biology and identifies a common genetic variant that may increase the risk of death in Black patients with heart failure.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)