Article
Biochemistry & Molecular Biology
Amanda C. Carita, Rafaela R. M. Cavalcanti, Mariana S. S. Oliveira, Karin A. Riske
Summary: This study investigates the solubilization of lipid vesicles by various non-ionic detergents and compares the results with a standard detergent. The results show that only TX-100 and OG are able to completely solubilize the vesicles.
CHEMISTRY AND PHYSICS OF LIPIDS
(2023)
Article
Biotechnology & Applied Microbiology
Ngoc T. N. Ngo, Javier A. Linares-Pasten, Carl Grey, Patrick Adlercreutz
Summary: The study focuses on the synthesis of new anionic alkyl glycosides with long carbohydrate chains using laccase/TEMPO oxidation and CGTase catalysis approaches. The efficiencies and product stabilities of the two methods varied, with laccase/TEMPO oxidation showing depolymerization issues, while CGTase catalysis yielding high conversions especially when using Toruzyme. pH was found to have a strong influence on enzyme activity and acceptor specificity, with optimal pH ranges differing for non-ionic and acidic substrates.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Agriculture, Multidisciplinary
Fang Fu, Yulin Fan, Langqiu Chen, Jing Zhang, Jiping Li, Jingyi Liao, Guochao Zhang
Summary: A series of nonionic disaccharide-based surfactants were synthesized and their physicochemical properties were investigated. The surfactants demonstrated good water solubility, surface activity, foaming ability, and emulsifying property, suggesting their potential applications in related fields.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kazi Zubaida Gulshan Ara, Javier A. Linares-Pasten, Jonas Jonsson, Maria Viloria-Cols, Stefan Ulvenlund, Patrick Adlercreutz, Eva Nordberg Karlsson
Summary: The mutations of F197, G263, and E266 in CGTase CspCGT13 affect its interactions with acceptor substrates and coupling activity. The double mutant F197Y/G263A displayed enhanced coupling activity, while the triple mutant F197Y/G263A/E266A maintained the increase in primary coupling product. These findings suggest the importance of specific mutations in enzyme engineering for improved surfactant production.
Review
Pharmacology & Pharmacy
Stuart Madden, Enrique Carrazana, Adrian L. Rabinowicz
Summary: Intranasal delivery of drugs provides advantages in ease of use, rapid onset, and patient experience, making it particularly relevant for patients with acute events related to central nervous system (CNS) conditions. Adaptation of intranasal formulations to the nasal cavity's anatomical and physiological characteristics, including limited dose volume, restricted surface area, and mucosal absorption barriers, is crucial. The addition of alkylsaccharide permeation enhancers, such as dodecyl maltoside (DDM), can optimize drug absorption within the constraints of nasal delivery, as demonstrated by FDA-approved intranasal medications for epilepsy, acute opioid overdose, and migraine.
Article
Biology
Ido Caspy, Maria Fadeeva, Yuval Mazor, Nathan Nelson, David M. Kramer
Summary: High-resolution cryo-EM structures of eukaryotic PSII from Dunaliella salina revealed different conformations and demonstrated the role of CP29 in distinguishing the conformers. Additionally, the study discovered the flexibility of PSII in different orientations, providing a flexible stacking mechanism for grana arrangement in thylakoids.
Article
Nanoscience & Nanotechnology
Yixin Zhang, Le You, Pu Deng, Xiaocheng Jiang, Huan-Hsuan Hsu
Summary: Utilizing exoelectrogens and bioreduced graphene oxide (B-rGO) as linkers, a biohybrid electronic material was designed and fabricated to enhance interaction between exoelectrogen community and external electronics. This material can also serve as a basic building block for constructing various electronic components with other microorganisms. Studies show that photosynthetic organelles and cells can replace exoelectrogens as active bioreducing components, leading to significantly increased photocurrent intensities in formed materials.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Yixin Zhang, Le You, Pu Deng, Xiaocheng Jiang, Huan-Hsuan Hsu
Summary: Exoelectrogens have the ability to reduce extracellular electron acceptors and form conductive nanomaterials integrated with their cell bodies. In this study, we designed and fabricated a biohybrid electronic material using bioreduced graphene oxide as the linker, enabling interaction between the exoelectrogen community and external electronics. This material can be effectively applied in microbial fuel cells and as building blocks for various electronic components. By replacing exoelectrogens with photosynthetic organelles and cells, the resulting materials showed significant improvements in photocurrent intensities.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Luis Morales-Quintana, Angela Mendez-Yanez
Summary: The cell wall plays a crucial role in providing turgor, protection against pathogen attacks, and structural support to cells. Changes in the cell wall structure and composition during fruit ripening can affect fruit shelf life. In this review, the focus is on exploring the role of alpha-mannosidase and beta-d-N-acetylhexosaminidase enzymes in fruit ripening and fruit firmness.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Applied
Vladimir B. Sheinin, Olga M. Kulikova
Summary: This study introduces a pH-controlled solubilization technique for hydrophobic porphyrin photosensitizers, utilizing anionic amphiphilic nanoparticles, which show increased fluorescence in acidic tumor microenvironments. This concept demonstrates the potential of pH-dependent off-on photosensitizers for photodynamic theranostics, with high fluorescence and phototoxicity within tumors and low toxicity in normal tissues.
Article
Plant Sciences
Fang Huang, Arturas Grauslys, Tuomas Huokko, Eva Caamano Gutierrez, Andrew R. Jones, Lu-Ning Liu
Summary: This study provides an in-depth statistical analysis of protein components and functionally linked complexes during cyanobacterial thylakoid membrane biogenesis. The findings reveal significant variations in abundance of photosystems, light-harvesting antennae, ABC transporters, and oxidative stress response and protein folding pathway enzymes between high light and growth light conditions. The results and analytical methodologies developed in this study may be valuable for studying global responses of thylakoid membrane biogenesis in plants and algae.
Article
Multidisciplinary Sciences
James L. Martin Robinson, Willem K. Kegel
Summary: Hydrophobic polyelectrolytes (HPEs) can solubilize bilayer membranes, form micelles, or reversibly aggregate with pH changes. These cooperative transitions can be explained using Monod- Wymann-Changeux (MWC) theory, originally formulated for allosteric interactions. In this study, we focus on the pH-dependent destabilization and permeation of bilayer membranes by HPEs and present general conditions for sharp conformational transitions mediated by concentration variations of molecular ligands. This opens potential applications in fields such as medicine and switchable materials.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Prahlad Singh, Deepak Sharma, Sumit Singhal, Ajeet Kumar, Ashwani Kumar Singh, Mrigank Honparkhe
Summary: Adding SDS 0.125%, NOG 0.0625 mM, and 4-MPG 3.125 mM to TEYG buffalo semen extender significantly improved the kinetic and functional parameters of post-thaw spermatozoa.
Review
Cell Biology
Marine Messant, Anja Krieger-Liszkay, Ginga Shimakawa
Summary: Photosynthesis relies on efficient regulation mechanisms to prevent damage to the photosynthetic apparatus and aid in acclimation to changing environments. Protein movements, supercomplex formation, and changes in oligomerization states play crucial roles in these regulatory processes.
Article
Biochemistry & Molecular Biology
Wenjing Lu, Yaping Fang, Xue Meng, Xiaoli Wang, Wenbo Liu, Mengdong Liu, Ping Zhang
Summary: As a tumor-targeting oncolytic adenovirus, conditionally replicating adenovirus (CRAd) can specifically replicate and destroy cancer cells. The use of 6-cyclohexyl methyl-beta-D-maltoside (6-beta-D) as a transfection agent improves the transduction efficiency of CRAd and enhances its antitumor effect. Pretreatment of cancer cells with low concentration of 6-beta-D significantly increases the transduction efficiency of CRAd, while being safe for normal cells.
Article
Energy & Fuels
Samir Bensaid, Bernardo Ruggeri, Guido Saracco
Article
Chemistry, Physical
Sitaramanjaneya Mouli Thalluri, Simelys Hernandez, Samir Bensaid, Guido Saracco, Nunzio Russo
APPLIED CATALYSIS B-ENVIRONMENTAL
(2016)
Meeting Abstract
Gastroenterology & Hepatology
Michele Pier Luca Guarino, Candida Vannini, Annamaria Altomare, Simone Barera, Vittoria Locato, Silvia Cocca, Giorgio Arrigoni, Rossana Alloni, Laura De Gara, Paola Campomenosi, Michele Cicala
Article
Biochemical Research Methods
Simone Cristoni, Guglielmo Dusi, Paolo Brambilla, Adriana Albini, Matteo Conti, Maura Brambilla, Antonino Bruno, Francesca Di Gaudio, Luca Ferlin, Valeria Tazzari, Silvia Mengozzi, Simone Barera, Carlos Sialer, Tommaso Trenti, Marco Cantu, Luigi Rossi Bernardi, Douglas M. Noonan
JOURNAL OF MASS SPECTROMETRY
(2017)
Article
Multidisciplinary Sciences
Michele Pier Luca Guarino, Annamaria Altomare, Simone Barera, Vittoria Locato, Silvia Cocca, Cinzia Franchin, Giorgio Arrigoni, Candida Vannini, Sarah Grossi, Paola Campomenosi, Valentina Pasqualetti, Marcella Bracale, Rossana Alloni, Laura De Gara, Michele Cicala
Article
Plant Sciences
Andrea Trotta, Simone Barera, Francesco Marsano, Domenico Osella, Davide Musso, Cristina Pagliano, Flora Andreucci, Roberto Barbato
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Review
Biotechnology & Applied Microbiology
Manuel Benedetti, Valeria Vecchi, Simone Barera, Luca Dall'Osto
MICROBIAL CELL FACTORIES
(2018)
Article
Biotechnology & Applied Microbiology
Luca Dall'Osto, Stefano Cazzaniga, Zeno Guardini, Simone Barera, Manuel Benedetti, Giuseppe Mannino, Massimo E. Maffei, Roberto Bassi
BIOTECHNOLOGY FOR BIOFUELS
(2019)
Review
Plant Sciences
Valeria Vecchi, Simone Barera, Roberto Bassi, Luca Dall'Osto
Article
Plant Sciences
Edoardo Cutolo, Matteo Tosoni, Simone Barera, Luis Herrera-Estrella, Luca Dall'Osto, Roberto Bassi
Article
Biotechnology & Applied Microbiology
Manuel Benedetti, Simone Barera, Paolo Longoni, Zeno Guardini, Natalia Herrero Garcia, David Bolzonella, Damar Lopez-Arredondo, Luis Herrera-Estrella, Michel Goldschmidt-Clermont, Roberto Bassi, Luca Dall'Osto
Summary: A new platform for expressing multiple thermostable cell wall degrading enzymes in algae chloroplasts has been developed, enabling the conversion of alkaline-treated lignocellulose into glucose with high efficiencies. The hydrolysates from the treated cellulosic materials boosted biogas production by methanogenic bacteria and mixotrophic growth of oil-rich microalgae.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Zeno Guardini, Luca Dall'Osto, Simone Barera, Mehrdad Jaberi, Stefano Cazzaniga, Nicola Vitulo, Roberto Bassi
Summary: By applying chemical mutagenesis and selecting for norflurazon-resistant strains, enhanced carotenoid pool size and growth tolerance under photooxidative stress were observed in Chlorella vulgaris. Optimizing carotenoid/chlorophyll ratio and resistance to photooxidative stress improved light-to-biomass conversion efficiency under high light conditions of photobioreactors. Enhanced tolerance to singlet oxygen in Chlorella is promoted by at least two independent mechanisms, only one of which is carotenoid-dependent.
Article
Biotechnology & Applied Microbiology
Edoardo Cutolo, Matteo Tosoni, Simone Barera, Luis Herrera-Estrella, Luca Dall'Osto, Roberto Bassi
Summary: Photosynthetic microalgae have great potential as hosts for expressing heterologous proteins. This study developed a self-reinforcing genetic system in Chlamydomonas reinhardtii to enhance the reliability of plastid-based algal expression platforms. The use of a bifunctional transgene and phosphite as a selective agent facilitated efficient expression of hydrolytic enzymes in axenic algal cultures.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Review
Biotechnology & Applied Microbiology
Simone Barera, Giuseppe Forlani
Summary: Microalgae are a promising source for renewable fuels, high-value bio-products, and nutraceuticals. However, their productivity in large-scale cultivation is often limited by sub-optimal growth conditions and sensitivity to environmental changes. Proline accumulation plays a role in stress adaptation in higher plants, but its implication in microalgae remains to be fully understood. This review summarizes the available data on proline metabolism in eukaryotic microalgae under environmental stress and discusses its potential implications for optimizing algal growth for biotechnological purposes.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Simone Barera, Luca Dall'Osto, Roberto Bassi
Summary: The study revealed that reducing photoprotective energy dissipation in Chlamydomonas algae does not effectively increase biomass productivity in photo-bioreactors. One strain lacking LHCSR1 and knocked down in LHCSR3 showed higher oxygen production under high light, along with enhanced PSII photodamage. Contrary to previous proposals, domestication of Chlamydomonas for carbon assimilation rate in photo-bioreactors by down-regulation of photoprotective energy dissipation was ineffective in increasing algal biomass productivity.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)