Article
Biochemistry & Molecular Biology
Elianne P. Bulthuis, Cindy E. J. Dieteren, Jesper Bergmans, Job Berkhout, Jori A. Wagenaars, Els M. A. van de Westerlo, Emina Podhumljak, Mark A. Hink, Laura F. B. Hesp, Hannah S. Rosa, Afshan N. Malik, Mariska Kea-te Lindert, Peter H. G. M. Willems, Han J. G. E. Gardeniers, Wouter K. den Otter, Merel J. W. Adjobo-Hermans, Werner J. H. Koopman
Summary: Macromolecules of various sizes induce crowding in the cellular environment, which affects biochemical reactions by altering solvent viscosity, water-accessible volume, and protein properties. This study shows that the mitochondrial matrix solvent exhibits macromolecular crowding, as demonstrated by the elongated molecular structure of fluorescent protein fusion peptides and decreased diffusion constant with increasing molecular weight. Chloramphenicol treatment impairs mitochondrial function and increases matrix viscosity, indicating increased macromolecular crowding. These findings highlight the importance of matrix crowding in mitochondria and suggest that changes in crowding conditions can impact biochemical reactions.
Article
Materials Science, Paper & Wood
Aref Abbasi Moud, Amir Sanati-Nezhad, Seyed Hossein Hejazi
Summary: This study evaluates the stability and self-healing ability of cellulose nanocrystal hydrogels using quantitative fluorescence signal analysis. Hydrogel samples with lower cellulose nanocrystal concentrations collapse more rapidly under gravity, while increasing concentration inhibits particle mobility and hinders the self-healing process.
Article
Infectious Diseases
Xiu-Gang Guan, Shi-Xia Zhou, An -Ran Zhang, Qing-Bin Lu, Zi-Wei Zhou, Jin-Jin Chen, Hai -Yang Zhang, Yang Ji, Bao-Gui Jiang, Yang Yang, Zhi-Cong Yang, Yue-Hong Wei, Hao Li, Li-Qun Fang, Wei Liu
Summary: This study compared the efficacy of different antibiotics in the treatment of severe scrub typhus. The results showed that azithromycin and doxycycline had significant therapeutic effects in patients with severe scrub typhus, while chloramphenicol was less efficacious.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Chemistry, Applied
Yahui Guo, Panting Sang, Gang Lu, Xue Yang, Yunfei Xie, Zhigang Hu, He Qian, Weirong Yao
Summary: An artificial deoxyribozyme was used as a substitute for horseradish peroxidase (or alkaline phosphatase) in ELISA for generating amplified signals. The feasibility of the proposed deoxyribozyme-based ELISA (DLISA) was demonstrated in the detection of a forbidden veterinary drug, chloramphenicol. With a detection limit of 0.1 ng/L and a wide linear range from 0.000001 μg/mL to 1.0 μg/mL, DLISA showed excellent practical performances. The easy connection of antibody and DNAzyme through gold nanoparticles in DLISA made it worth popularizing.
Article
Chemistry, Applied
Javier M. Abbas, Martin Stortz, Hernan B. Rodriguez, Valeria Levi, Alejandro Wolosiuk, Carla C. Spagnuolo
Summary: In this study, three novel near-infrared aminotricarbocyanine derivatives with antioxidant moieties gallic acid and Trolox were synthesized and investigated for their photophysical properties. The dyes showed enhanced photostability in aqueous and non-aqueous environments due to a self-healing process through redox quenching of reactive intermediates from excited states. Additionally, a functional group (-COOH) was included in the chemical structure of the dyes for potential conjugation with biomolecules or surfaces.
Article
Spectroscopy
Yuexia Zhang, Xiaopeng Fan, Xuansen Sun, Xintong Yang, Zhongping Li, Zhenhua Yang, Chuan Dong
Summary: In this study, oil-soluble carbon dots were successfully fabricated and applied for the detection of doxycycline in milk. These carbon dots showed high sensitivity and potential for real sample applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Chemistry, Analytical
Shuang Han, Xuan Zhang, Hongda Sun, Jinping Wei, Hui Wang, Shuangyu Wang, Jing Jin, Zhichao Zhang
Summary: The study presents a method for the direct and quantitative determination of CAP and DOX using a GCE modified with carboxylic-group-functionalized single-walled carbon nanohorns. The modified electrode shows high sensitivity and linear response towards both substances, making it suitable for real sample analysis.
Article
Chemistry, Inorganic & Nuclear
Jiawei Cheng, Nana Liu, Luyao Wang, Huaiwei Wang, Jing Lu, Yunwu Li, Jianmin Dou, Suna Wang
Summary: A novel three-dimensional Cd-MOF LCU-117 was constructed, which exhibited a unique helical structure and had high selectivity and sensitivity towards Eu3+ ions. LCU-117 could detect antibiotic oxytetracycline (OTC) through the turn-off mechanism, and the detection capability was significantly enhanced when Eu3+ was added. LCU-117 showed promising applications in logic gates, potential fingerprint detection, and mixed-matrix membranes (MMMs), and provided satisfactory results in monitoring OTC in water samples.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yao Yao, Jingyu Kuang, Jiahe Ju, Tao Hu, Wei Shen, Sheng Tang, Chang Liu, Dezhao Kong, Haiwei Shi, Hian Kee Lee
Summary: A simple fluorescence sensing device based on solvent-free magnetic-tip microextraction was developed and successfully applied for the fluorescence detection of chloramphenicol (CAP) in genuine milk and urine samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Pengfei Ma, Hualin Guo, Nuo Duan, Xiaoyuan Ma, Lin Yue, Qianhui Gu, Zhouping Wang
Summary: The study showed that ionic strength and type influence the recognition of aptamers, with magnesium ion playing a vital role in the binding process. Various methods were used to analyze the binding performance and conformational changes of the aptamer, leading to the development of a label-free fluorescence polarization sensor for detecting chloramphenicol.
Article
Food Science & Technology
Yinyin Chen, Xini Wang, Changfang Lu, Wei Wu, Xianxiang Wang
Summary: This study reported a simple and rapid method for detecting DOX in food samples using a ratiometric fluorescence approach based on BSA-Au NCs. The BSA-Au NCs probe exhibited a good linear range and low detection limit for DOX. The research demonstrated the potential application of BSA-Au NCs in the food field due to its convenient synthesis and excellent selectivity and sensitivity.
Article
Pharmacology & Pharmacy
Ardiyah Nurul Fitri Marzaman, Sartini, Mukarram Mudjahid, Tri Puspita Roska, Anwar Sam, Andi Dian Permana
Summary: This study successfully incorporated chloramphenicol into whey protein and formulated it into a thermoresponsive in situ gel for wound healing treatment, demonstrating promising properties in terms of bioadhesivity, skin occlusivity, and antibacterial activity.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Xiaoxiao Chen, Yang Liu, Pu Li, Yichen Xing, Chaobiao Huang
Summary: A method for the trace detection of chloramphenicol (CAP) was established using a dual recognition system with a fluorescence quenching of quantum dots (QDs) and specific recognition of molecularly imprinted polymer (MIP). The MIP@SiO2@QDs showed good detection selectivity for CAP and was successfully applied to quantify CAP in lake water with promising potential for on-site detection in environmental waters.
Article
Biophysics
Takumi Saito, Daiki Matsunaga, Shinji Deguchi
Summary: Fluorescence recovery after photobleaching (FRAP) is a technique used to analyze molecular turnover in living cells. However, existing models have limitations in interpreting long-term FRAP responses due to the additional effects of cellular movement. To overcome this, researchers have developed a continuum mechanics-based model that decouples FRAP response into turnover rate and subcellular mechanical characteristics.
BIOPHYSICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Shuyi Huang, Long Yu, Pengchen Su, Tao Wen, Mingtai Sun, Dejian Huang, Xiangke Wang, Suhua Wang
Summary: In this study, a new type of fluorescent organosilicon nanodots (OSiNDs) was successfully constructed and used for the on-site detection of antibiotic doxycycline residue. The fluorescence of doxycycline was enhanced through conformational fixation on the surface of OSiNDs, resulting in fluorescence resonance energy transfer (FRET). The optimized method provided a sensitive and selective detection of doxycycline based on ratiometric fluorescence signal.
ANALYTICA CHIMICA ACTA
(2022)
Article
Engineering, Biomedical
Jing Xie, Min Bao, Xinyu Hu, Werner J. H. Koopman, Wilhelm T. S. Huck
Summary: Cells respond to mechanical cues from the extracellular matrix by adjusting their cytoskeleton and forming focal adhesions, which can lead to changes in ATP levels and activation of AMPK. This can affect cell spreading and tension, as well as influence stem cell fate in response to ECM mechanics. Lowered ATP levels on softer substrates limit cellular mechanoresponses.
Review
Clinical Neurology
Melissa A. E. van de Wal, Merel J. W. Adjobo-Hermans, Jaap Keijer, Tom J. J. Schirris, Judith R. Homberg, Mariusz R. Wieckowski, Sander Grefte, Evert M. van Schothorst, Clara van Karnebeek, Albert Quintana, Werner J. H. Koopman
Summary: Mitochondrial dysfunction is associated with various syndromes and diseases, including Leigh syndrome. Mutations in the NDUFS4 gene can cause Leigh syndrome, and studying it in mouse models has provided new insights into the pathomechanism and treatment.
Review
Cell Biology
Sandeep Chenna, Werner J. H. Koopman, Jochen H. M. Prehn, Niamh M. C. Connolly
Summary: This article discusses the mechanistic models and minimal models of mitochondrial reactive oxygen species (ROS) homeostasis. Mechanistic models provide valuable insights but are complex, while minimal models use a smaller set of equations to describe empirical relationships and generate hypotheses. Finally, the article illustrates the application of minimal models in studying the pathophysiology of ROS.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Koen van den Dries, Jack Fransen, Alessandra Cambi
Summary: Correlative light and electron microscopy (CLEM) is a powerful imaging approach that combines light and electron microscopy, and has wide applications in biology. This review summarizes the important developments in CLEM for biological research, with a focus on the combination of fluorescence microscopy and electron microscopy.
Article
Biochemistry & Molecular Biology
Elianne P. Bulthuis, Claudia Einer, Felix Distelmaier, Laszlo Groh, Sjenet E. Van Emst-De Vries, Els Van De Westerlo, Melissa Van De Wal, Jori Wagenaars, Richard J. Rodenburg, Jan A. M. Smeitink, Niels P. Riksen, Peter H. G. M. Willems, Merel J. W. Adjobo-Hermans, Hans Zischka, Werner J. H. Koopman
Summary: The study showed that targeted molecules for mitochondria can lead to decreased levels of mitochondrial complex I, reduced ETC supercomplexes, inhibition of mitochondrial oxygen consumption, increased extracellular acidification, altered mitochondrial morphology, and stimulated hydroethidine oxidation. The research highlights the impact of mitochondria-targeting moieties on cellular functions.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Elianne P. Bulthuis, Cindy E. J. Dieteren, Jesper Bergmans, Job Berkhout, Jori A. Wagenaars, Els M. A. van de Westerlo, Emina Podhumljak, Mark A. Hink, Laura F. B. Hesp, Hannah S. Rosa, Afshan N. Malik, Mariska te Lindert, Peter H. G. M. Willems, Wouter den Otter, Han J. G. E. Gardeniers, Merel J. W. Adjobo-Hermans, Werner J. H. Koopman
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Jan Willem Borst, Jack Fransen, Stefanie Weidtkamp-Peters, Yvonne Stahl
Article
Neurosciences
Malou Zuidscherwoude, Mark K. K. van Goor, Sara R. R. Roig, Niky Thijssen, Merijn van Erp, Jack Fransen, Jenny van der Wijst, Joost G. G. Hoenderop
Summary: This study characterizes the interaction between functional TRPV5 and CaM, demonstrating that they interact in the absence of Ca2+ and the interaction is intensified with increasing Ca2+ concentrations. The CaM C-lobe achieves channel pore blocking, leading to channel inactivation. Furthermore, the study shows a Ca2+-dependent binding stoichiometry at the single channel level. This research proposes a new CaM-dependent regulation model for TRPV5, involving apoCaM interaction and lobe-specific actions, which is of significant physiological relevance given its role in Ca2+ transport in the kidney.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Microbiology
Samara Rosendo Machado, Jieqiong Qu, Werner J. H. Koopman, Pascal Miesen
Summary: This study discovered the important role of Dhx15 gene and the glycolysis pathway it regulates in controlling mosquito infection by arboviruses, providing a new perspective on the molecular interactions between arboviruses and their mosquito hosts.
Article
Toxicology
Charlotte A. Hoogstraten, Maaike M. E. Jacobs, Guido de Boer, Melissa A. E. van de Wal, Werner J. H. Koopman, Jan A. M. Smeitink, Frans G. M. Russel, Tom J. J. Schirris
Summary: Mitochondrial dysfunction plays a pivotal role in drug-induced acute kidney injury (AKI) and the transport protein AAC is an important potential drug off-target. In this study, AAC3 deficiency led to reduced mitochondrial function and mass in human renal proximal tubular cells, particularly affecting metabolic spare capacity under galactose conditions.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Elianne P. Bulthuis, Cindy E. J. Dieteren, Jesper Bergmans, Job Berkhout, Jori A. Wagenaars, Els M. A. van de Westerlo, Emina Podhumljak, Mark A. Hink, Laura F. B. Hesp, Hannah S. Rosa, Afshan N. Malik, Mariska Kea-te Lindert, Peter H. G. M. Willems, Han J. G. E. Gardeniers, Wouter K. den Otter, Merel J. W. Adjobo-Hermans, Werner J. H. Koopman
Summary: Macromolecules of various sizes induce crowding in the cellular environment, which affects biochemical reactions by altering solvent viscosity, water-accessible volume, and protein properties. This study shows that the mitochondrial matrix solvent exhibits macromolecular crowding, as demonstrated by the elongated molecular structure of fluorescent protein fusion peptides and decreased diffusion constant with increasing molecular weight. Chloramphenicol treatment impairs mitochondrial function and increases matrix viscosity, indicating increased macromolecular crowding. These findings highlight the importance of matrix crowding in mitochondria and suggest that changes in crowding conditions can impact biochemical reactions.
Article
Biochemistry & Molecular Biology
Federica Conte, Angel Ashikov, Rachel Mijdam, Eline G. P. van de Ven, Monique van Scherpenzeel, Raisa Veizaj, Seyed P. Mahalleh-Yousefi, Merel A. Post, Karin Huijben, Daan M. Panneman, Richard J. T. Rodenburg, Nicol C. Voermans, Alejandro Garanto, Werner J. H. Koopman, Hans J. C. T. Wessels, Marek J. Noga, Dirk J. Lefeber
Summary: Phosphoglucomutase 1 (PGM1) is a key enzyme involved in energy metabolism and its deficiency causes a wide range of symptoms. D-galactose supplementation has been shown to improve some symptoms, but its effects on skeletal muscle and heart remain unclear. In this study, a muscle cell model for PGM1 deficiency was established, which revealed that D-galactose failed to restore the reduced energy production capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Medicine, General & Internal
D. W. J. H. Koopman
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)