Article
Biochemistry & Molecular Biology
Zhongming Li, Shan Tang, Zhi Shi, Bin Li, Dong Feng, Delong Xie, Tao Han, Chengyong Li
Summary: Large bone defects pose a challenge in orthopedic treatments, but tissue-engineered scaffolds provide new possibilities. However, the clinical use of such scaffolds has been limited by inadequate mechanical properties and osteogenesis. This study proposes a biocompatible PLA-based composite with nano-hydroxyapatite to mimic cancellous bone's morphology, along with Mg2+ and chitosan as active osteogenic factors. The resulting scaffold has a bionic structure, favorable mechanical properties, and induces efficient osteogenic differentiation, making it a promising strategy for bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Ali Salehi, Mohammad A. Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The study investigated the characteristics of decellularized cabbage leaves and their potential for promoting bone differentiation, as demonstrated by enhanced osteogenic differentiation of BM-MSCs on the scaffold. These results suggest that decellularized cabbage leaves have great potential for tissue engineering applications in bone regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Materials Science, Ceramics
Patricia Ros-Tarraga, Carlos M. Martinez, Miguel A. Rodriguez, Piedad N. De Aza
Summary: The elderly population is increasing, leading to an increase in bone and joint problems. Scaffold for bone tissue regeneration can solve these problems, and material selection is crucial for satisfactory results. This study designed a three-dimensional porous ceramic scaffold and analyzed its biocompatibility and osteoinductive capacity. The results suggest that it has potential applications as bone substitutes.
CERAMICS INTERNATIONAL
(2022)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Dana Jenkins, Karim Salhadar, Grant Ashby, Anita Mishra, Joy Cheshire, Felipe Beltran, Melissa Grunlan, Sebastien Andrieux, Cosima Stubenrauch, Elizabeth Cosgriff-Hernandez
Summary: The pore size of a scaffold has significant effects on various physical properties in tissue engineering. Manual measurements using ImageJ/FIJI are time-consuming and subjective. In this study, a new MATLAB algorithm called PoreScript was developed and validated using different scaffold fabrication methods. The results demonstrate the accuracy and versatility of PoreScript.
BIOACTIVE MATERIALS
(2022)
Article
Cell Biology
Sandra Stamnitz, Agnieszka Krawczenko, Urszula Szalaj, Zaneta Gorecka, Agnieszka Antonczyk, Zdzislaw Kielbowicz, Wojciech Swieszkowski, Witold Lojkowski, Aleksandra Klimczak
Summary: FGF-2 and BMP-2 enhance the osteogenic differentiation potential of MSCs grown on a scaffold, making them promising tools for bone regeneration. This was confirmed through in vitro and in vivo experiments.
Article
Engineering, Biomedical
Guang Yang, Bhushan Mahadik, Ji Young Choi, Justine R. Yu, Trevor Mollot, Bin Jiang, Xiaoming He, John P. Fisher
Summary: The study utilized a microfluidic approach to prepare collagen microbeads loaded with human bone marrow derived mesenchymal stem cells for creating engineered bone grafts. The cell-laden microbeads self-assembled into macro-tissues, enabling precise control over cell spatial distribution at the macroscale, offering flexibility for graft volume and architecture. The approach has the potential to create 3D engineered bone grafts with customized vasculature using modular tissue engineering and 3D printing.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Thakoon Thitiset, Siriporn Damrongsakkul, Supansa Yodmuang, Wilairat Leeanansaksiri, Jirun Apinun, Sittisak Honsawek
Summary: The novel biodegradable scaffold comprising gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) showed promising biological characteristics for bone tissue engineering both in vitro cell culture and in vivo animal bioassays.
BIOMATERIALS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Luciana Sartore, Chiara Pasini, Stefano Pandini, Kamol Dey, Marco Ferrari, Stefano Taboni, Harley H. L. Chan, Jason Townson, Sowmya Viswanathan, Smitha Mathews, Ralph W. Gilbert, Jonathan C. Irish, Federica Re, Piero Nicolai, Domenico Russo
Summary: This paper introduces a novel process to develop composite scaffolds with a core-shell structure for bone tissue regeneration. The core provides temporary mechanical support, while the shell enhances biocompatibility and provides bioactive properties. The hybrid polymer devices closely mimic the morphology and stiffness of bones, and can efficiently support the growth and differentiation of human mesenchymal stromal cells towards the osteogenic phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Engineering, Biomedical
Liaobing Xin, Xiaowen Zheng, Jianmin Chen, Sentao Hu, Yilun Luo, Qunzi Ge, Xiaoying Jin, Lie Ma, Songying Zhang
Summary: This study explores an acellular biomaterial called SDF-1 alpha/E7-modified collagen scaffold (CES), which enables near-complete endometrium regeneration and fertility restoration by mobilizing endogenous MSCs. The results demonstrate that CES can overcome the traditional limitations of cell-based therapies and serve as a clinically feasible and cell-free strategy with high therapeutic efficiency for IUA treatment.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Polymer Science
Janicy Arantes Carvalho, Cristiano Ceron Jayme, Flavia Sayuri Matsuo, Antonio Claudio Tedesco
Summary: Extensive injuries to bone tissue are still a significant clinical challenge, necessitating the development of new bone tissue engineering strategies. This study successfully constructed a chitosan-gelatin/hydroxyapatite-based scaffold to support the growth and differentiation of mesenchymal stem cells into osteoblasts. The scaffold exhibited high biocompatibility and significantly enhanced bone lesion closure in in vivo studies.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Review
Cell Biology
Venkata Suresh Venkataiah, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Yusuke Kakiuchi, Masato Nakano, Shigeto Suzuki, Keisuke Handa, Masahiro Saito
Summary: Bone tissue engineering involves combining MSCs with a biocompatible scaffold to aid in bone regeneration and recovery. While promising results have been seen in clinical trials, regenerating large bone defects remains a challenge.
Article
Biochemistry & Molecular Biology
W. Benton Swanson, Maiko Omi, Seth M. Woodbury, Lindsey M. Douglas, Miranda Eberle, Peter X. Ma, Nan E. Hatch, Yuji Mishina
Summary: This study investigated the impact of scaffold pore curvature on the differentiation trajectory of mesenchymal stem cells (MSCs), demonstrating that the curvature of scaffold pores can influence cell aggregation and differentiation by altering the cytoskeleton, ultimately guiding cell fate specification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Yamini Chandramohan, Kavya Jeganathan, Sanjana Sivanesan, Pavani Koka, Trikkur Madom Seetaraman Amritha, Selvaraj Vimalraj, Anuradha Dhanasekaran
Summary: This study successfully isolated mesenchymal stem cells (MSCs) from human Ovarian Follicular Fluid (OFF) for the first time, and characterized them through various methods. The growth of MSCs with the conditioned medium obtained from a biocomposite scaffold promoted osteoblast differentiation, indicating good culture conditions and applicability.
Article
Biochemistry & Molecular Biology
Helen Power, Jiadai Wu, Stuart Turville, Anupriya Aggarwal, Peter Valtchev, Aaron Schindeler, Fariba Dehghani
Summary: The COVID-19 pandemic has highlighted the need for new antivirals. Using virtual screening, this study identified compounds with antiviral activity against the SARS-CoV-2 spike protein. This validates virtual screening as a tool for discovering potential antivirals.
BIOORGANIC CHEMISTRY
(2022)
Review
Engineering, Biomedical
Sepehr Talebian, Timothy Schofield, Peter Valtchev, Aaron Schindeler, John M. Kavanagh, Qayyum Adil, Fariba Dehghani
Summary: The potential health benefits of probiotics may not be realized due to their reduced viability during storage and gastrointestinal transit. Microencapsulation using biopolymers has been utilized to improve the resistance of probiotics to critical conditions. However, most existing methods only provide single layer protection, which is less effective than more sophisticated approaches. This review discusses emerging methods for multilayer encapsulation of probiotics using biopolymers, and examines the correlation between fabrication techniques and microparticle properties. Novel materials and approaches for multilayer structures are highlighted.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Endocrinology & Metabolism
Lucinda R. Lee, Aimee E. Holman, Xiaoying Li, Emily R. Vasiljevski, Alexandra K. O'Donohue, Tegan L. Cheng, David G. Little, Aaron Schindeler, Andrew Biggin, Craig F. Munns
Summary: This preclinical study investigated the effects of human growth hormone (hGH) and zoledronic acid (ZA) on bone quality in mice with osteogenesis imperfecta (OI). The results showed that hGH alone increased bone length in wild-type mice, while the combination of hGH/ZA increased bone length in both wild-type and OI mice. MicroCT analysis revealed that hGH/ZA treatment increased cortical bone density and thickness. ZA had a greater impact on trabecular bone, but hGH rescued bone turnover. However, these improvements in bone quality did not translate into improvements in mechanical strength.
Article
Chemistry, Applied
Katherine Blackshaw, Jiadai Wu, Nicholas Proschogo, Justin Davies, Daniel Oldfield, Aaron Schindeler, Richard B. Banati, Fariba Dehghani, Peter Valtchev
Summary: This study evaluated the application of freeze-drying and low-dose gamma irradiation for long-term storage of donor human milk (DHM). The results showed that this method effectively preserved the quality of DHM and could be a safe and convenient way to store and deliver it at ambient temperature, making it suitable for emergencies and humanitarian aid.
Article
Orthopedics
Alexandra K. O'Donohue, Aiken Dao, Justin D. Bobyn, Craig F. Munns, David G. Little, Aaron Schindeler
Summary: Osteogenesis imperfecta (OI), a genetic bone fragility disorder, can be treated with bone morphogenetic proteins (BMPs) and bisphosphonates (BPs). However, the use of BMPs as local agents for fracture healing in OI may be less effective, while the combination of BMP-2 and BPs shows considerable improvements in bone volume and polar moment of inertia. Therefore, caution should be taken when using BMP-2 alone in OI surgical settings, but the off-label administration of BPs may help overcome this limitation.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Endocrinology & Metabolism
Alexandra K. O'Donohue, Ya Xiao, Lucinda R. Lee, Timothy Schofield, Tegan L. Cheng, Craig F. Munns, Paul A. Baldock, Aaron Schindeler
Summary: In this study, a bone-targeted recombinant adeno-associated virus (AAV) vector was used to efficiently produce postnatal skeletal knockout mice using gene-floxed strains. The AAV8-Sp7-Cre vector induced widespread and persistent recombination in the bone compartment, resulting in significant increases in bone volume and strength. This research confirms the important postnatal role of the Sost gene in regulating bone homeostasis and identifies a novel role in canalicular remodeling.
Article
Nanoscience & Nanotechnology
Matthew McGrath, Karolina Zimkowska, Katelyn J. Genoud, Jack Maughan, Javier Gutierrez Gonzalez, Shane Browne, Fergal J. O'Brien
Summary: A biomimetic, bilayered antimicrobial collagen-based scaffold was developed to deal with the etiology of diabetic foot ulcers (DFUs). The scaffold exhibited high structural stability, successfully inhibited the growth and infiltration of Staphylococcus aureus, and supported the proliferation of epidermal cells and vascularization. These results suggest that the bilayered scaffold is a promising candidate for enhancing diabetic wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Claudio Intini, Lia Blokpoel Ferreras, Sarah Casey, James E. Dixon, John P. Gleeson, Fergal J. O'Brien
Summary: A novel miR-activated scaffold was developed to enhance mesenchymal stem/stromal cells (MSC) chondrogenesis and cartilage repair through the delivery of an inhibitor to miR-221. The miR-activated scaffold successfully transfected human MSCs with the miR-221 cargo, promoting an improved cell-mediated chondrogenic response. This innovative scaffold shows promise in improving chondrogenesis and enhancing cartilage defect repair.
ADVANCED THERAPEUTICS
(2023)
Article
Infectious Diseases
Aiken Dao, Alexandra K. O'Donohue, Emily R. Vasiljevski, Justin D. Bobyn, David G. Little, Aaron Schindeler
Summary: This study developed a reliable and cost-effective murine bone infection model that mimics bacterial bone infections associated with biofilm and metal implants. The tibial drilled hole (TDH) and needle insertion surgery (NIS) infection models were compared, and both achieved a 100% infection rate when a metal implant was present. The NIS model was simpler to perform than the TDH model.
JOURNAL OF BONE AND JOINT INFECTION
(2023)
Review
Cell Biology
Helen Power, Peter Valtchev, Fariba Dehghani, Aaron Schindeler
Summary: This article discusses the classification of senolytic drugs and methods for screening new drugs. Researchers have discovered a range of senolytic drugs and introduce their different categories and mechanisms of action. The article also highlights the need for further research into drug targets and mechanisms, as well as rigorous evaluation in pre-clinical models and human trials.
Article
Nanoscience & Nanotechnology
Giuseppe A. Asaro, Matteo Solazzo, Meenakshi Suku, Dahnan Spurling, Katelyn Genoud, Javier Gutierrez Gonzalez, Fergal J. O' Brien, Valeria Nicolosi, Michael G. Monaghan
Summary: Electroconductive biohybrid platforms were created by blending collagen and 2D MXene, which showed high biocompatibility and enhanced cell proliferation and spreading. The platforms also limited bacterial attachment and proliferation. Culturing neonatal rat cardiomyocytes and human induced pluripotent stem cell-derived cardiomyocytes on the substrates demonstrated increased cell growth and cx43 expression when stimulated with an external electric field. This in vitro study convincingly shows the potential of the engineered conductive biohybrid platform for cardiac tissue regeneration.
NPJ 2D MATERIALS AND APPLICATIONS
(2023)
Article
Rheumatology
Sohel M. Julovi, Aiken Dao, Katie Trinh, Alexandra K. O'Donohue, Cindy Shu, Susan Smith, Meena Shingde, Aaron Schindeler, Natasha M. Rogers, Christopher B. Little
Summary: This study developed a preclinical comorbid model of chronic kidney disease (CKD) and osteoarthritis (OA), and investigated the disease-modifying interactions between these two diseases. The findings demonstrated bidirectional disease-modifying interaction between CKD and OA.
Review
Engineering, Biomedical
Tara K. Mcguire, Martyna Stasiewicz, Ian Woods, Adrian G. Dervan, Fergal J. O'Brien
Summary: Spinal cord injury is a devastating traumatic injury that often results in permanent loss of function. Gene therapy using nonviral nanoparticle vectors has the potential to improve spinal cord regeneration by targeting multiple aspects of the injury and enhancing specificity. Despite challenges, nanoparticle-mediated gene delivery holds great promise for the future of spinal cord injury treatment.
ADVANCED NANOBIOMED RESEARCH
(2023)
Article
Endocrinology & Metabolism
V. A. Shahen, A. Schindeler, M. S. Rybchyn, C. M. Girgis, B. Mulholland, R. S. Mason, I. Levinger, T. C. Brennan-Speranza
Summary: Patients with type 2 diabetes mellitus have a higher risk of fractures despite normal to high bone mineral density. This study investigated the effects of high glucose levels on human osteoblasts and tested the efficacy of parathyroid hormone and cinacalcet in promoting cell growth and matrix mineralization. The findings suggest that combination therapy with these two agents may ameliorate the negative impact of chronic high blood glucose on bone remodeling.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Review
Urology & Nephrology
Sorcha O'Meara, Eoghan M. Cunnane, Stefanie M. Croghan, Connor V. Cunnane, Michael T. Walsh, Fergal J. O'Brien, Niall F. Davis
Summary: The ureter is a complex structure with varying mechanical properties and morphology along its anatomical length. Understanding the mechanical forces and properties of the ureter is important for surgical outcomes and urine transport. Further studies on human ureteric tissue are needed to better understand these properties and the influence of forces on them.
NATURE REVIEWS UROLOGY
(2023)