期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1787, 期 6, 页码 584-592出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2009.02.003
关键词
Complex I; Iron-sulfur cluster; Pulsed EPR; REFINE; Hyperfine spectroscopy
资金
- Sonderforschungsbereich [SFB 472]
- Center for Biomolecular Magnetic Resonance (BMRZ)
Electron Paramagnetic Resonance (EPR) spectroscopy is the method of choice to study paramagnetic cofactors that often play an important role as active centers in electron transfer processes in biological systems. However, in many cases more than one paramagnetic species is contributing to the observed EPR spectrum, making the analysis of individual contributions difficult and in some cases impossible. With time-domain techniques it is possible to exploit differences in the relaxation behavior of different paramagnetic species to distinguish between them and separate their individual spectral contribution. Here we give an overview of the use of pulsed EPR spectroscopy to study the iron-sulfur clusters of NADH:ubiquinone oxidoreductase (complex I). While FeS cluster NI can be studied individually at a temperature of 30 K, this is not possible for FeS cluster N2 due to its severe spectral overlap with cluster N1. In this case Relaxation Filtered Hyperfine (REFINE) spectroscopy can be used to separate the overlapping spectra based on differences in their relaxation behavior. (C) 2009 Elsevier B.V. All rights reserved.
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