期刊
BIOCHEMISTRY-MOSCOW
卷 78, 期 2, 页码 157-165出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297913020041
关键词
protein biosynthesis; translational control; cellular IRES-elements; cap-independent translation; Apaf-1 mRNA
资金
- Russian Foundation for Basic Research [11-04-01010_a, 12-04-32039-mol_a]
- State Contract of Ministry of Education and Science of Russian Federation [14.740.11.0168]
We have earlier shown that the 5'-untranslated region (5' UTR) of the mRNA coding for activation factor of apoptotic peptidase 1 (Apaf-1) can direct translation in vivo by strictly 5' end-dependent way even in the absence of m(7)G-cap. Dependence of translational efficiency on the cap availability for this mRNA turned out to be relatively low. In this study we demonstrate that this surprising phenomenon is determined the 5'-proximal part (domains I and II) of highly structured Apaf-1 5' UTR. Remarkably, domain II by itself was able to reduce dependence of the mRNA on the cap on its transferring to a short 5' UTR derived from a standard vector. We suggest that the low cap-dependence inherent to some cellular mRNAs may have an important physiological significance under those stress conditions when the function of cap-binding factor eIF4E is impaired.
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