期刊
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
卷 37, 期 1, 页码 11-15出版社
JOHN WILEY & SONS INC
DOI: 10.1002/bmb.20243
关键词
Enzymes and catalysis; medical biochemistry; sources of difficulties and teaching strategies to correct; difficulties
Most general biochemistry textbooks present enzyme inhibition by showing how the basic Michaelis-Menten parameters Km and Vi are affected mathematically by a particular type of inhibitor. This approach, while mathematically rigorous, does not lend itself to understanding how inhibition patterns are used to determine the kinetic aspects of an enzyme. The discussion here describes a qualitative approach to teaching enzyme inhibition that allows for a physical or mechanistic understanding. This qualitative approach to enzyme inhibition starts by recognizing that the two fundamental kinetic parameters of an enzyme catalyzed reaction are V(max) and V(max)/K(m), which correspond to the apparent rates of reaction at very high and very low concentrations of substrate, respectively. It just so happens that the reciprocals of V(max) and V(max)/K(m) correspond to the y-intercept and slope of the Lineweaver-Burk plot, respectively. Thus, an inhibitor that affects the y-intercept binds to the enzyme at very high substrate concentrations, and thus binds to the enzyme-substrate complex, while an inhibitor that affects the slope binds to the enzyme at very low substrate concentrations, and thus binds only to free enzyme. These simple precepts can be used to interpret the basic inhibition patterns, competitive, uncompetitive and noncompetitive, and more importantly, derive mechanistic information, especially in multisubstrate reactions. The application of these principles is illustrated by using an example from cancer chemotherapy, the inhibition of thymidylate synthase by 5-fluorouracil and leucovorin.
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