Article
Hematology
Amer Youssef, Justin R. Clark, Santica M. Marcovina, Michael B. Boffa, Marlys L. Koschinsky
Summary: This study reveals the noncovalent interaction between apoB and apo(a) within hepatocytes and its impact on Lp(a) biosynthesis. These findings contribute to the understanding of the results from human metabolic studies and the mechanisms of drugs that lower Lp(a) levels.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Nutrition & Dietetics
Mohsen Mazidi, Richard J. Webb, Elena S. George, Niloofar Shekoohi, Julie A. Lovegrove, Ian G. Davies
Summary: Individuals with discordantly high apoB to LDL-cholesterol levels had the highest intake of NP1 and the lowest intake of NP2. Machine learning results indicated that total fat, SFA, and thiamine had the greatest unfavorable dietary contribution to individuals with discordantly high apoB to LDL-cholesterol, while MUFA, folate, fiber, and Se had the greatest favorable contributions. Individuals with discordantly high apoB compared to LDL-cholesterol were more likely to adhere to NP1, while those with lower levels of apoB tended to consume NP3.
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Mikhail Rudinskiy, Maurizio Molinari
Summary: Conserved catabolic pathways operate to remove aberrant polypeptides from the endoplasmic reticulum (ER), utilizing ERAD pathways and ER-phagy. ERAD pathways control retrotranslocation of misfolded proteins across the ER membrane, while ER-phagy segregates proteins into ER subdomains that eventually vesiculate. These ER-derived vesicles can be captured by autophagosomes, endolysosomes/vacuoles, or fused with degradative organelles.
Article
Medicine, Research & Experimental
Xiaoan Wei, Zeyu Zheng, Zhenhua Feng, Lin Zheng, Siyue Tao, Bingjie Zheng, Bao Huang, Xuyang Zhang, Junhui Liu, Yilei Chen, Wentian Zong, Zhi Shan, Shunwu Fan, Jian Chen, Fengdong Zhao
Summary: Sigmar1 is a negative regulator of osteoclastogenesis and activation of Sigmar1 by dimemorfan may be a potential treatment for osteoporosis.
EMBO MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Carmen Rodriguez-Jimenez, Gema de la Pena, Javier Sanguino, Sara Poyatos-Pelaez, Ana Carazo, Pedro L. Martinez-Hernandez, Francisco Arrieta, Jose M. Mostaza, Diego Gomez-Coronado, Sonia Rodriguez-Novoa
Summary: Mutations in APOB are the second most frequent cause of familial hypercholesterolemia (FH). It is necessary to perform functional analysis to determine the pathogenicity of APOB variants due to its high polymorphism. In this study, APOB variants were identified and characterized in patients with hypercholesterolemia. Two variants, p.(Lys3344Glu) and p.(Ser3801Thr), were studied in detail. The p.(Lys3344Glu) variant was shown to be causative of FH, while the p.(Ser3801Thr) variant was found to be benign.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jiantao Zhang, Ruth Cruz-Cosme, Chenyu Zhang, Dongxiao Liu, Qiyi Tang, Richard Y. Zhao
Summary: The ORF3a protein of SARS-CoV-2 is crucial in the viral pathogenesis and COVID-19. This study demonstrates two distinct types of ORF3a proteins based on their subcellular localization and reveals the functional and mechanistic disparities between them.
Review
Spectroscopy
Erica Gianazza, Beatrice Zoanni, Alice Mallia, Maura Brioschi, Gualtiero Colombo, Cristina Banfi
Summary: This narrative review highlights the potential of lipoprotein-associated proteins in cardiovascular risk assessment and emphasizes the importance of high-throughput proteomic analysis.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Medicine, Research & Experimental
Subramaniyan Divya, Palaniyandi Ravanan
Summary: The endoplasmic reticulum (ER) is a dynamic organelle responsible for correctly folding proteins. Various internal and external factors can disrupt its function and trigger ER stress responses. The unfolded protein response (UPR) mechanism and ER-associated degradation systems help to reduce misfolded proteins and maintain cell survival, while communication with other organelles and stress signaling pathways regulate cell survival or cell death. The UPR can be targeted for therapeutic purposes and used as a biomarker for diseases.
Article
Cell Biology
Helene Tourriere, Karim Chebli, Latifa Zekri, Brice Courselaud, Jean Marie Blanchard, Edouard Bertrand, Jamal Tazi
Summary: This study reveals that G3BP is a key player in stress granule (SG) assembly, and Ras signaling regulates G3BP dephosphorylation to promote SG formation. SGs are formed in the cytoplasm in response to various toxic agents and are believed to play a critical role in regulating mRNA metabolism during stress. The findings suggest that G3BP determines the fate of mRNAs during cellular stress and Ras signaling is involved in this process through regulating G3BP dephosphorylation.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Plant Sciences
Hong Du, Canqi Zheng, Muhmmad Aslam, Xihui Xie, Wanna Wang, Yingquan Yang, Xiaojuan Liu
Summary: The study revealed the responses of C. reinhardtii under high lead stress in physiological, genomic, transcriptomic, glycomic, and bioinformatic aspects, indicating that the endoplasmic reticulum-mediated pathways related to protein quality control were activated, potentially leading to the reduction of protein level.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Shuangcheng Alivia Wu, Chenchen Shen, Xiaoqiong Wei, Xiawei Zhang, Siwen Wang, Xinxin Chen, Mauricio Torres, You Lu, Liangguang Leo Lin, Huilun Helen Wang, Allen H. Hunter, Deyu Fang, Shengyi Sun, Magdalena I. Ivanova, Yi Lin, Ling Qi
Summary: Endoplasmic reticulum-associated degradation (ERAD) and ER-phagy are two central degradative mechanisms in the ER. This study describes the sequence of events underlying the disposition of misfolded ER proteins by ERAD and ER-phagy.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Jichen Chen, Hong Du, Zidong Liu, Tangcheng Li, Hua Du, Wanna Wang, Muhammad Aslam, Weizhou Chen, Ping Li, Haodong Luo, Hao Fang, Xiaojuan Liu
Summary: This study investigated the effects of tunicamycin stress on the marine diatom Phaeodactylum tricornutum. The results showed that tunicamycin stress significantly inhibited cell growth and photosynthesis, and caused alterations in carbon and nitrogen metabolisms. Additionally, the study enriched the N-glycan database of P. tricornutum and provided important information for studying the function of N-glycosylation modification on proteins.
MICROBIAL CELL FACTORIES
(2022)
Article
Virology
Florian Hinte, Jendrik Mueller, Wolfram Brune
Summary: The Unfolded Protein Response (UPR) and Endoplasmic Reticulum-Associated Degradation (ERAD) play crucial roles in protein quality control in the secretory pathway. Viruses like cytomegaloviruses modulate the UPR to benefit their replication by degrading IRE1, a sensor molecule for UPR. The viral M50 protein degrades IRE1 using the ERAD machinery, facilitating virus replication.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hui Luo, Qi-Bin Jiao, Chuan-Bin Shen, Wen-Yan Gong, Jing-Hua Yuan, Ying-Ying Liu, Zhen Chen, Jiang Liu, Xiao-Ling Xu, Yu-Sheng Cong, Xing-Wei Zhang
Summary: Ubiquitin fold modifier 1 (UFM1) is a small ubiquitin-like protein modifier that is essential for embryonic development. This study reveals that UFM1 modifies HRD1, a ubiquitin ligase involved in ER-associated protein degradation (ERAD), and facilitates ERAD function to maintain ER homeostasis.
Review
Endocrinology & Metabolism
Srividhya Iyer, Douglas J. Adams
Summary: The synthesis and maintenance of a healthy proteome are crucial for the functionality of osteoblasts and osteoclasts. Impaired and altered secretion of skeletal cells can lead to various skeletal diseases. The Unfolded Protein Response (UPR) is a signaling cascade initiated by three ER membrane proteins to remediate the accumulation of misfolded proteins and regulate the cellular proteome. However, sustained activation of UPR due to chronic ER stress can result in cell death and contribute to skeletal diseases like osteoporosis. Future mechanistic studies should focus on developing UPR therapeutics to mitigate adverse skeletal outcomes.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Hamideh Ghazizadeh, Mary Kathryn Bohn, Mahdiyeh Yaghooti-Khorasani, Roshanak Ghaffarian-Zirak, Mohsen Valizadeh, Maryam Saberi-Karimian, Hamideh Safarian, Atieh Kamel-Khodabandeh, Reza Zare-Feyzabadi, Ameneh Timar, Maryam Mohammadi-Bajgiran, Mohammad Reza Oladi, Meysam Gachpazan, Mohadeseh Rohban, Habibollah Esmaily, Gordon A. Ferns, Khosrow Adeli, Majid Ghayour-Mobarhan
Summary: The study found that there were no significant age and sex-specific differences in most biomarkers, except for uric acid which had higher concentrations in men. Only hs-CRP levels were positively associated with higher BMI among participants with a BMI above 25. Reference intervals for PAB and anti-hsp-27 in men and women aged 35-65 were reported. The partitioning by sex and BMI was only required for uric acid and hs-CRP, while other biomarkers required no partitioning.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Nicole M. A. White-Al Habeeb, Victoria Higgins, Allison A. Venner, Dana Bailey, Daniel R. Beriault, Christine Collier, Khosrow Adeli
Summary: There is limited guidance on laboratory reporting and interpretation of lipids and lipoproteins used in cardiovascular risk stratification, leading to inconsistencies in lipid reporting across clinical laboratories. The Canadian Cardiovascular Society has published guidelines aligned with harmonized lipid reporting recommendations for improving standardization of lipid assessment and clinical decision-making.
CANADIAN JOURNAL OF CARDIOLOGY
(2022)
Article
Endocrinology & Metabolism
Elisabeth M. Grande, Fitore Raka, Simon Hoffman, Khosrow Adeli
Summary: This study reports a novel role for neuronal nitric oxide synthase (nNOS)-mediated nitric oxide (NO) generation in lipid uptake and chylomicron formation, which has important implications for postprandial dyslipidemia associated with insulin-resistant states such as obesity and type 2 diabetes.
Article
Biochemistry & Molecular Biology
Cristian Sandoval, Luciana Mella, Karina Godoy, Khosrow Adeli, Jorge Farias
Summary: The objective of this study was to investigate the antioxidant effect of oral beta-carotene intake on CYP2E1 gene expression in mice previously exposed to ethanol. The results suggest that beta-carotene may increase liver damage caused by low and high doses of alcohol.
Article
Medical Laboratory Technology
Ruth Ronn, Mary Kathryn Bohn, Ellen M. Greenblatt, Barry Hoffman, Khosrow Adeli
Summary: This study aims to establish accurate reference intervals for anti-mullerian hormone (AMH) in pediatric/adolescent survivors to assess their reproductive potential. The study found differences in AMH concentrations among females of different age groups but these differences were unlikely to be clinically meaningful. Menstrual status and ethnicity did not significantly impact AMH concentrations.
CLINICAL BIOCHEMISTRY
(2022)
Article
Medical Laboratory Technology
Mary Kathryn Bohn, Siobhan Wilson, Randal Schneider, Youssef Massamiri, Edward W. Randell, Khosrow Adeli
Summary: This study verified the performance of the Alinity i assays in clinical laboratory investigation of autoimmune, metabolic, and oncologic disorders in children and adolescents. It also confirmed the transferability of the pediatric reference intervals established in the CALIPER cohort for 16 specialized immunoassays from the ARCHITECT system to the Alinity system.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Lusia Sepiashvili, Mary Kathryn Bohn, Alexandra Hall, Tina Henderson, Jack Chen, Roseline Dunst, Khosrow Adeli
Summary: The objective of this study was to establish pediatric reference limits for autoimmune disease markers in healthy children and adolescents. Serum autoantibody testing was conducted on a BIO-FLASH analyzer and reference limits were calculated using the non-parametric rank method. The results showed a proportion of samples with results above the lower limit of the analytical measuring range for each marker. The study concluded that the established reference limits will improve laboratory assessment and clinical decision making in pediatric patients using the BIO-FLASH assay platform worldwide.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Review
Microbiology
Nyan Abdalqadir, Khosrow Adeli
Summary: The intestine plays a crucial role in maintaining metabolic homeostasis, energy expenditure, and overall human health by sensing and responding to various signals, harboring a large population of microbes, and secreting a wide variety of hormones and molecules to regulate nutrition and physiology.
Letter
Cardiac & Cardiovascular Systems
Victoria Higgins, Nicole White-Al Habeeb, Khosrow Adeli
CANADIAN JOURNAL OF CARDIOLOGY
(2023)
Article
Medical Laboratory Technology
Rainer Haeckel, Khosrow Adeli, Graham Jones, Ken Sikaris, Werner Wosniok
Summary: This paper proposes a general definition covering all indirect models currently in use. With the increasing popularity of indirect models, further partitioning strategies are necessary to minimize the risk of patients' false classifications. Indirect methods make these partitions much easier to execute compared to direct methods. The authors believe that the future of reference interval estimation lies in indirect models with big data pools from either one laboratory or combined from several regional centres.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Siobhan M. Wilson, Mary Kathryn Bohn, Andre Madsen, Thomas Hundhausen, Khosrow Adeli
Summary: This study establishes continuous reference percentiles for 14 biomarkers in a healthy pediatric population using the LMS model. The LMS-based approach improves test result interpretation, especially with repeated measures over time.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Medical Laboratory Technology
Pyper Olver, Mary Kathryn Bohn, Khosrow Adeli
Summary: Clinical laboratories play a vital role in the healthcare system. The International Federation for Clinical Chemistry and Laboratory Medicine (IFCC) has established a task force to support research on the role of laboratory medicine in clinical outcomes. This review discusses the contribution of laboratory medicine in diagnosing and managing heart diseases, sepsis, traumatic brain injury, and the fight against the COVID-19 pandemic.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Cell Biology
Niloufar Abdollahian, Hamideh Ghazizadeh, Maryam Mohammadi-Bajgiran, Mehran Pashirzad, Mahdiyeh Yaghooti Khorasani, Mary Kathryn Bohn, Shannon Steele, Fatemeh Roudi, Atieh Kamel Khodabandeh, Sara Ghazi Zadeh, Iman Alami-Arani, Seyede Negin Badakhshan, Habibollah Esmaily, Gordon A. Ferns, Reza Assaran-Darban, Khosrow Adeli, Majid Ghayour-Mobarhan
Summary: Age-specific reference intervals for 7 laboratory biochemical parameters were determined in an Iranian pediatric population. These findings are valuable for the clinical interpretation of blood test results and disease management in this population.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Hematology
Mary Kathryn Bohn, Siobhan Wilson, Shannon Steele, Khosrow Adeli
Summary: The current study aimed to establish pediatric reference intervals (RIs) for both common and novel hematology parameters on the Mindray BC-6800Plus system. Dynamic reference value distributions were observed for several parameters, emphasizing the complex biological patterns of hematology parameters in childhood, particularly at the onset of puberty. These findings support the need for age- and sex-specific RIs for appropriate clinical interpretation.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Review
Medicine, General & Internal
Giuseppe Lippi, Brandon M. Henry, Khosrow Adeli, Mario Plebani
Summary: This study provides a pooled analysis of the accuracy of the Fujirebio Lumipulse SARS-CoV-2 Antigen chemiluminescent immunoassay for diagnosing acute SARS-CoV-2 infections. The analysis shows that this method has good diagnostic sensitivity and specificity, making it a valuable option for complementing and integrating with molecular testing for SARS-CoV-2 in the current pandemic.