期刊
BIOCHEMISTRY
卷 53, 期 42, 页码 6667-6678出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi5007207
关键词
-
资金
- National Natural Science Foundation of China [31172150, 31272375, 31301881]
- Ministry of Science and Technology of China [2012CB910402]
Tachykinins constitute one of the largest peptide families in the animal kingdom and exert their diverse actions via G protein-coupled receptors (GPCRs). In this study, the Bombyx tachykinin-related peptides (TKRPs) were identified as specific endogenous ligands for the Bombyx neuropeptide GPCR A24 (BNGR-A24) and thus designated BNGR-A24 as BmTKRPR. Using both mammalian cell line HEK293 and insect cell line Sf21, further characterization demonstrated that BmTKRPR was activated, thus resulting in intracellular accumulation of cAMP, Ca2+ mobilization, and ERK1/2 phosphorylation in a G(s) and G(q) inhibitor-sensitive manner. Moreover, quantitative reverse transcriptase polymerase chain reaction analysis and dsRNA-mediated knockdown experiments suggested a possible role for BmTKRPR in the regulation of feeding and growth. Our findings enhance the understanding of the Bombyx TKRP system in the regulation of fundamental physiological processes.
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