期刊
BIOCHEMISTRY
卷 53, 期 24, 页码 3940-3951出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi500148y
关键词
-
资金
- National Institutes of Health [GM084266]
- Robert A. Welch Foundation [A1170]
The majority of Fe in Fe-replete yeast cells is located in vacuoles. These acidic organelles store Fe for use under Fe-deficient conditions and they sequester it from other parts of the cell to avoid Fe-associated toxicity. Vacuolar Fe is predominantly in the form of one or more magnetically isolated nonheme high-spin (NHHS) Fe-III complexes with polyphosphate-related ligands. Some Fe-III oxyhydroxide nanoparticles may also be present in these organelles, perhaps in equilibrium with the NHHS Fe-III. Little is known regarding the chemical properties of vacuolar Fe. When grown on adenine-deficient medium (A down arrow), ADE2 Delta strains of yeast such as W303 produce a toxic intermediate in the adenine biosynthetic pathway. This intermediate is conjugated with glutathione and shuttled into the vacuole for detoxification. The iron content of A down arrow. W303 cells was determined by Mossbauer and EPR spectroscopies. As they transitioned from exponential growth to stationary state, A down arrow. cells (supplemented with 40 mu M Fe-III citrate) accumulated two major NHHS Fe-II species as the vacuolar NHHS Fe-III species declined. This is evidence that vacuoles in A down arrow, cells are more reducing than those in adenine-sufficient cells. A down arrow. cells suffered less oxidative stress despite the abundance of NHHS Fe-II complexes; such species typically promote Fenton chemistry. Most Fe in cells grown for days with extra yeast-nitrogen-base, amino acids and bases in minimal medium was HS Fe-III with insignificant amounts of nanoparticles. The vacuoles of these cells might be more acidic than normal and can accommodate high concentrations of HS Fe-III species. Glucose levels and rapamycin (affecting the TOR system) affected cellular Fe content. This study illustrates the sensitivity of cellular Fe to changes in metabolism, redox state and pH. Such effects broaden our understanding of how Fe and overall cellular metabolism are integrated.
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