Article
Veterinary Sciences
Li-jun Guan, Jin-qian Yang, Qing-yuan Xu, Yi-fan Feng, Xi-chen Zhang, Bo Tang, Zhan-qin Zhao
Summary: This study investigated the protective effects of serogroup A and D bacterins against current circulating P. multocida strains. Results showed that D: L6 strains provided effective cross-protection against parent strains, while cross-protectivity of serogroup A strains was poor. This provides some insights for P. multocida vaccine development.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Shubhant Pandey, Bryan W. Berger, Rudresh Acharya
Summary: Anionic polysaccharides found in nature and the corresponding polysaccharide lyases (PLs) exhibit functional and structural convergence. pH affects substrate binding and catalytic activity, and specific groups of substrates are cleaved in different pH ranges regardless of the PL fold, supporting the concept of functional convergence. This study reveals the structural determinants of conserved substrate-pH activity pairing within and between PL families.
Article
Biotechnology & Applied Microbiology
Sabine Schelch, Manuel Eibinger, Stefanie Gross Belduma, Barbara Petschacher, Juergen Kuballa, Bernd Nidetzky
Summary: This study demonstrates the advantages of using the synthase reaction over the lyase reaction for 3’-sialyllactose synthesis, leading to increased yield and productivity. The research also shows model-based optimization to minimize protein loading while achieving target yield and productivity for oligosaccharide production.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Qi An, Si Chen, Luyin Zhang, Zhenxing Zhang, Yiwen Cheng, Haotian Wu, Ang Liu, Zhen Chen, Bin Li, Jie Chen, Yiying Zheng, Churiga Man, Fengyang Wang, Qiaoling Chen, Li Du
Summary: This study investigates the characteristics of the host immune response induced by P. multocida strains of serotype A and D. The results revealed significant changes in gene and miRNA expression related to immunity in the bronchial epithelial cells stimulated by these two serotypes. Additionally, specific GO terms and KEGG pathways associated with immunity were identified. The study suggests that the serotype D strain may induce a cellular immune response earlier than the serotype A strain. Overall, this study provides valuable insights into the host immune response mechanism induced by P. multocida strains.
Article
Biochemistry & Molecular Biology
Chen Wang, Qingjie Xiao, Huaichuan Duan, Jinhong Li, Jiying Zhang, Qisheng Wang, Li Guo, Jianping Hu, Bo Sun, Dong Deng
Summary: Nucleoside homeostasis mediated by transporters and channels is essential for life, and NupG in Escherichia coli serves as a prototype for nucleoside transport. The crystal structures of NupG and its uridine binding site provide a framework for understanding the mechanism of nucleoside transporters in the NHS family.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Takfarinas Kentache, Leopold Thabault, Gladys Deumer, Vincent Haufroid, Raphael Frederick, Carole L. Linster, Alessio Peracchi, Maria Veiga-da-Cunha, Guido T. Bommer, Emile Van Schaftingen
Summary: N-acetylneuraminate (Neu5Ac) is a sugar commonly found in vertebrates and some bacteria, serving as an energy source for certain prokaryotes like Escherichia coli. The aldolase NanA in E. coli and its human homolog NPL were found to preferentially metabolize the open form of Neu5Ac. Additionally, the Neu5Ac anomerase NanM was able to stimulate the utilization of both beta and alpha-anomers by NanA in vitro.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Fei Xu, Fang Dong, Xiao-Hui Sun, Hai-Yan Cao, Hui-Hui Fu, Chun-Yang Li, Xi-Ying Zhang, Andrew McMinn, Yu-Zhong Zhang, Peng Wang, Xiu-Lan Chen
Summary: Ulvan is an important marine polysaccharide and bacterial ulvan lyases play crucial roles in its degradation. A new ulvan lyase, Uly1, belonging to polysaccharide lyase family 24, from the marine bacterium Catenovulum maritimum has been characterized. The study provides new insights into ulvan lyases and their degradation mechanisms.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Luyao Wang, Xue Han, Yulu Wang, Xue Wei, Shujun Liu, Shuli Shao, Shaoqing Yang, Lichao Sun, Fengjiao Xin
Summary: This study successfully enhanced the activity and substrate specificity of gut bacteria-derived acetyl xylan esterase through rational design and mutation. Mutants N65S and N65A showed larger substrate binding pockets, leading to improved activity towards different length substrates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Qiong Zhang, Jiao Zhang, Yanhong Shao, Guangdong Shang
Summary: In this study, the highest biotransformation yield of N-acetyl-d-neuraminic acid (Neu5Ac) was obtained by using the AGE gene BT0453 cloned from the human gut microorganism Bacteroides thetaiotaomicron VPI-5482. Deletion of Escherichia coli chromosomal genes involved in substrate degradation, product exportation, and pH change, as well as precursor (pyruvate) feeding, further improved the yield of Neu5Ac. These findings highlight the significance of host optimization and provide a foundation for future metabolic engineering of Neu5Ac biotransformation.
BIOTECHNOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Jiemin Shen, Miaohui Hu, Xiao Fan, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming Zhou
Summary: This study reveals the role of ECD in PepT1, confirming its importance in protein function, and providing new insight into the transport mechanism of PepT1.
Article
Biochemistry & Molecular Biology
Joshua M. Mitchell, Cynthia M. June, Vincent L. Baggett, Beth C. Lowe, James F. Ruble, Robert A. Bonomo, David A. Leonard, Rachel A. Powers
Summary: The evolution of multidrug resistance in Acinetobacter spp. poses a risk to the efficacy of our best antibiotics. The carbapenem-hydrolyzing class D beta-lactamase subfamily found in Acinetobacter spp. is particularly concerning due to its ability to confer resistance to carbapenems. By analyzing the structure and kinetics of the carbapenem-hydrolyzing class D beta-lactamase OXA-24/40, we discovered its preference for ertapenem and increased catalytic efficiency. These findings provide valuable insights for future inhibitor design efforts.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
H. T. Henry Chan, Marc A. Moesser, Rebecca K. Walters, Tika R. Malla, Rebecca M. Twidale, Tobias John, Helen M. Deeks, Tristan Johnston-Wood, Victor Mikhailov, Richard B. Sessions, William Dawson, Eidarus Salah, Petra Lukacik, Claire Strain-Damerell, C. David Owen, Takahito Nakajima, Katarzyna Swiderek, Alessio Lodola, Vicent Moliner, David R. Glowacki, James Spencer, Martin A. Walsh, Christopher J. Schofield, Luigi Genovese, Deborah K. Shoemark, Adrian J. Mulholland, Fernanda Duarte, Garrett M. Morris
Summary: Through various biophysical and crystallographic data analysis, researchers investigated the molecular features underlying the binding of SARS-CoV-2's main protease to its natural cleavage sites, highlighting the importance of interactions between substrates and enzymes. They designed peptides with improved affinity to competitively inhibit M-pro, providing new insights and opportunities for the development of M-pro inhibitors as anti-COVID-19 drugs.
Article
Chemistry, Physical
Zsofia Bata, Zsofia Molnar, Erzsebet Madaras, Bence Molnar, Evelin Santa-Bell, Andrea Varga, Ibolya Leveles, Renzhe Qian, Friedrich Hammerschmidt, Csaba Paizs, Beata G. Vertessy, Laszlo Poppe
Summary: The study reveals that a small opening of the inner loop is sufficient for ligand egress in both eukaryotic aromatic amino acid ammonia-lyases and aminomutases, and the ligand-binding tunnels in these enzymes are analogous. Additionally, engineering of these binding tunnels can convert an (R)-aminomutase to a highly selective (S)-beta-lyase, establishing a novel enzyme function.
Article
Biochemistry & Molecular Biology
Michael A. Johnstone, Samantha J. Nelson, Christine O'Leary, William T. Self
Summary: Selenium is an important micronutrient in many organisms with both beneficial and toxic effects. It is chemically similar to sulfur, but evolution has favored its use in a subset of oxidoreductases. The specific incorporation of selenium into selenoproteins is not fully understood, and there are inherent differences in discriminatory mechanisms between bacterial and mammalian enzymes.
Article
Chemistry, Multidisciplinary
Victoria N. N. Drago, Claudia Campos, Mattea Hooper, Aliyah Collins, Oksana Gerlits, Kevin L. L. Weiss, Matthew P. P. Blakeley, Robert S. S. Phillips, Andrey Kovalevsky
Summary: The authors determined the structure of Thermus thermophilus SHMT using X-ray/neutron crystallography and proposed its catalytic mechanism. This is important for drug design and enzyme engineering.
COMMUNICATIONS CHEMISTRY
(2023)
Review
Medicine, Research & Experimental
Akhilesh, Ankit Uniyal, Anagha Gadepalli, Vineeta Tiwari, Meghana Allani, Deepak Chouhan, Obulapathi Ummadisetty, Nimisha Verma, Vinod Tiwari
Summary: Chemotherapy-induced neuropathic pain (CINP) is a common clinical complication associated with anti-cancer drugs, affecting nearly 68.1% of cancer patients undergoing chemotherapy. The involvement of TRPV1 in CINP signaling suggests that targeting this nociceptor with siRNA may provide effective therapeutic interventions for managing CINP.
Article
Biochemistry & Molecular Biology
Shivani Jaiswal, Akhilesh, Ankit Uniyal, Vinod Tiwari, Senthil Raja Ayyannan
Summary: Researchers have discovered a promising lead compound for the treatment of neuropathic pain by designing and synthesizing a series of compounds. The lead compound exhibited potent inhibition activity against both FAAH and MAGL, and showed good safety profile in vivo experiments.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Immunology
Ankit Uniyal, Akhilesh, Aaina Singh Rathore, Priyanka Kumari Keshri, Surya Pratap Singh, Sanjay Singh, Vinod Tiwari
Summary: This study suggests that inhibition of pan-Aurora kinase can effectively inhibit evoked and chronic ongoing pain in nerve-injured rats by reducing KIF-17 mediated NR2B activation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Agriculture, Multidisciplinary
Jiawei Meng, Yingying Zhu, Hao Wang, Hongzhi Cao, Wanmeng Mu
Summary: Human milk oligosaccharides (HMOs) are considered the new gold standard for premium infant formula due to their unique beneficial effects for infants. HMOs are a collection of unconjugated glycans, with over 200 distinct structures identified. The production of HMOs involves glycosylation using specific glycosyltransferases and nucleotide sugar donors.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Nanoscience & Nanotechnology
Saurabh Shah, Paras Famta, Vinod Tiwari, Arun K. Kotha, Rama Kashikar, Mahavir Bhupal Chougule, Young Hun Chung, Nicole F. Steinmetz, Mohammad Uddin, Shashi Bala Singh, Saurabh Srivastava
Summary: This article reviews and discusses the interplay between tumor microenvironment and the immunological cascade and how it can be utilized to develop nanoparticle-based cancer vaccines and immunotherapies. Nanoparticles and proteinaceous vaccines have enormous potential in cancer therapy.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Priyanka Bose, Manoj K. Jaiswal, Sumit K. Singh, Rakesh K. Singh, Vinod K. Tiwari
Summary: In nature, glycan chains called sialic acids or nuraminic acids cover most cells and have long been recognized for their therapeutic importance. Sialic acid-containing glycans play a crucial role on cell surfaces, modulating and mediating various cellular interactions. Understanding the interaction between sialo-proteins and their roles in vertebrates has had a significant impact on medicine.
CARBOHYDRATE RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Jun Yan, Jianghua Li, Zhifei Hu, Xiaoyao Ma, Yun Li, Xihuan Hu, Jinfeng Ye, Wei Wang, Renzhong Wan, Hongzhi Cao
Summary: An efficient enzymatic assembly strategy was developed for the synthesis of structurally well-defined sialylated chitooligosaccharides. Two enzyme modules were used to introduce N-acetyl lactosamine (LacNAc) units onto the chitooligosaccharides. The LacNAc grafted chitooligosaccharides were further modified with sialic acid to generate a total of 20 sialylated chitooligosaccharides.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Shivani Jaiswal, Vinod Akhilesh, Vinod Tiwari, Senthil Raja Ayyannan
Summary: The isatin-derived carbohydrazone, SIH 3, showed significant anti-nociceptive activity in the neuropathic pain model without altering locomotor activity. It also displayed excellent safety profile in acute oral toxicity study and exhibited antioxidant effect in oxidative stress induced by chronic constrictive injury.
PHARMACOLOGICAL REPORTS
(2023)
Article
Chemistry, Organic
Sunil Kumar, Mangal S. Yadav, Sumit K. Singh, Sanchayita Rajkhowa, Vinod K. Tiwari
Summary: A modular and reliable click approach was used to synthesize lactose- and galactose-coated calixarenecored G(1) and G(2) generation glycodendrimers efficiently. The characterization of the developed calixarene glycodendrimers was performed using extensive spectral analysis including NMR (H-1 and C-13), MS, IR, and SEC data.
Article
Biochemistry & Molecular Biology
Priyanka Bose, Anand K. Agrahari, Rajan Singh, Mala Singh, Sunil Kumar, Rakesh K. Singh, Vinod K. Tiwari
Summary: A click-inspired piperazine glycoconjugate was developed to synthesize water-soluble and biocompatible motifs. This study focused on designing and synthesizing versatile sugar-appended triazoles using "Click Chemistry", and evaluated their pharmacological properties on cyclin-dependent kinases (CDKs) and cytotoxicity on cancer cells through in silico and in vitro approaches, respectively. The galactose- and mannose-derived piperazine conjugates were identified as promising motifs. The galactosyl bis-triazolyl piperazine analogue 10b was found to be the most interactive with CDKs and showed significant anticancer activity.
CARBOHYDRATE RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Manoj K. Jaiswal, Vinod K. Tiwari
Summary: Click Chemistry is a modular, rapid, and reliable tool used for the regioselective 1,2,3-triazole forming [3+2] reaction of organic azide and terminal alkyne. It is widely explored in various emerging research domains. The intramolecular click chemistry, which involves metal-catalyzed cyclization, organo-catalyzed cyclization, and thermal-induced topochemical reaction, is less addressed compared to the intermolecular metal variant. Recent approaches on intramolecular azide-alkyne cycloaddition 'Click Chemistry' and their emerging applications in the development of diverse molecules are reported.
Article
Chemistry, Multidisciplinary
Manoj K. Jaiswal, Abhishek Gupta, Mangal S. Yadav, Vinay K. Pandey, Vinod K. Tiwari
Summary: A novel organocatalyzed [3+2] cycloaddition reaction has been developed for the construction of 1,5-disubstituted triazolyl glycoconjugates. The reaction involves the use of Schreiner thiourea organocatalysts to activate nitroolefins through double hydrogen bonding. This metal-free and acid-free synthetic protocol is operationally simple, regioselective, and complements the classical RuAAC catalyzed synthesis of 1,5-disubstituted 1,2,3-triazoles. By using catalytic amounts of the Schreiner thiourea organocatalyst, a wide range of organic azides can react with nitroolefins to produce diverse 1,5-disubstituted 1,2,3-triazoles in good yields with excellent regioselectivity.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Organic
Sumit K. Singh, Sunil Kumar, Mangal S. Yadav, Abhishek Gupta, Vinod K. Tiwari
Summary: This report describes a convenient method for the Cu(I)-catalyzed Sonogashira cross-coupling reaction of aryl/heteroaryl halides and alkynyl sugars using a glycohybrid ligand. The method has notable features, including low catalytic loading, cost-effectiveness, and a wide substrate scope.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Organic
Sumit K. Singh, Sunil Kumar, Mangal S. Yadav, Subrato Bhattacharya, Vinod K. Tiwari
Summary: This report describes a convenient Cu(I)-catalyzed tandem synthesis method for the efficient synthesis of dihydrophenanthridinediones and substituted isoquinolinones using glycosyl 1,2,3-triazole-based pyridinamide ligands. The method successfully forms biologically relevant heterocyclic scaffolds in high yields through intermolecular C-C cross-coupling followed by intramolecular cyclization. Notable features of this method include low catalytic loading, use of cost-effective and biocompatible ligands, high reaction yield, and easily accessible starting materials, making it a versatile protocol.
SYNTHESIS-STUTTGART
(2023)
Review
Chemistry, Organic
Mangal S. Yadav, Abhishek Gupta, Priyanka Bose, Anoop S. Singh, Prabhu P. Mohapatra, Vinod K. Tiwari
Summary: N-Acylbenzotriazoles are important synthons in organic synthesis and can serve as alternative acylating agents in various organic transformations. This review summarizes the preparation methods of N-Acylbenzotriazole derivatives and their diverse applications, highlighting their ability to conveniently synthesize biologically important organic compounds as alternative acylating agents. The significance of using benzotriazole ring cleavage methodology in compound synthesis is also emphasized.