Article
Biochemistry & Molecular Biology
Brandon E. Aubol, Joseph A. Adams
Summary: This article investigates the function of SRPK1 in the nucleus. The study demonstrates that SRPK1 can regulate gene expression during RNA splicing through its interaction with U1-70K. By modulating the interaction between U1-70K, SR proteins, and ESE, SRPK1 affects the alternative splicing of genes.
Article
Biochemistry & Molecular Biology
Julia Kamenz, Lendert Gelens, James E. Ferrell
Summary: The phosphorylation of mitotic proteins is bistable, regulated by both Cdk1 and PP2A-B55 in a bidirectional manner. The changes in PP2A-B55 activity serve as the ultimate trigger for mitotic entry and exit, indicating its crucial role in regulating cell cycle progression.
Article
Multidisciplinary Sciences
Miho Sanagi, Shoki Aoyama, Akio Kubo, Yu Lu, Yasutake Sato, Shogo Ito, Mitsutomo Abe, Nobutaka Mitsuda, Masaru Ohme-Takagi, Takatoshi Kiba, Hirofumi Nakagami, Filip Rolland, Junji Yamaguchi, Takato Imaizumi, Takeo Sato
Summary: This study reveals the regulatory mechanism of nitrogen on plant flowering and nutrient metabolism, identifies the key roles of FBH4 and SnRK1 in this process, and uncovers their interactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Dalma Menesi, Eva Klement, Gyorgyi Ferenc, Attila Feher
Summary: Plant Rho-type GTPases (ROPs) are versatile molecular switches involved in various signal transduction pathways, but their direct interaction with protein kinases is not well understood. Although phosphorylation may regulate ROPs as in animal counterparts, there is limited experimental evidence. The study focused on potential phosphorylation sites of AtROP1 and different ROP-kinases, revealing new insights into the phosphorylation regulation of ROPs.
Article
Biology
Jeannette Delva-Wiley, Ese S. Ekhator, Laquaundra L. Adams, Supriya Patwardhan, Ming Dong, Robert H. Newman
Summary: The effects of oxidation on the activity of PKA-C α towards different substrates were investigated in this study. The results showed that oxidation differentially affects the activity of PKA-C α towards different substrates. This study provides novel insights into the crosstalk between redox- and phosphorylation-dependent signaling pathways mediated by PKA and lays the foundation for future studies on the role of redox-dependent modification of kinase substrate selection in physiological and pathological states.
Review
Biology
Seung-Hyeon Seok
Summary: Protein phosphorylation, a crucial post-translational modification process, is regulated by protein kinases and protein phosphatases. These reversible enzyme activities provide a regulatory mechanism for diverse protein functions in cellular processes.
Article
Endocrinology & Metabolism
Carina Mello Guimaraes Meyers, Samuel D. Burciaga, Bora Faulkner, Parandis Kazemi, Jacob M. Cohn, Kim C. Mansky, Eric D. Jensen
Summary: The PRKD/HDAC5 axis plays a crucial role in osteoclast formation and regulation of skeletal dynamics.
Article
Chemistry, Medicinal
Christian Borgo, Luca Cesaro, Tsuyoshi Hirota, Keiko Kuwata, Claudio D'Amore, Thomas Ruppert, Renata Blatnik, Mauro Salvi, Lorenzo A. Pinna
Summary: CK2, a protein kinase with pleiotropic functions, is implicated in global human pathologies, particularly cancer. Selective inhibitors like CX4945 and GO289 show promise in controlling the CK2-dependent phosphoproteome.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Renfei Ma, Shangfu Li, Luca Parisi, Wenshuo Li, Hsien-Da Huang, Tzong-Yi Lee
Summary: Phosphorylation is a crucial mechanism for regulating protein activities, but experimental determination of kinase-specific phosphorylation sites is time-consuming and expensive. This study aims to create predictive models for understudied kinases by leveraging relevant sources of similarities. Results demonstrate that 82 out of 116 understudied kinases were predicted with adequate performance, proving the reliability of web-like predictive networks in capturing patterns in such kinases.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Mutsuki Amano, Yoko Kanazawa, Kei Kozawa, Kozo Kaibuchi
Summary: This study analyzed the interaction between Rho-kinase and MYPT1 and found that three short regions of MYPT1 close to the phosphorylation sites are crucial for the interaction with Rho-kinase. The phosphorylation levels of MYPT1 without these regions were reduced and only affected neighboring phosphorylation sites. Furthermore, the combination of pseudosubstrate (PS) and docking motifs (DMs) of MYPT1 was found to be a potent inhibitor of Rho-kinase.
Article
Biochemistry & Molecular Biology
Ryan Kilburn, Eric T. Fedosejevs, Devang Mehta, Faranak Soleimani, Mina Ghahremani, Jacqueline Monaghan, Jay J. Thelen, R. Glen Uhrig, Wayne A. Snedden, William C. Plaxton
Summary: In this study, it was found that AtCPK4 and AtCPK11 positively regulate ABA signal transduction in Arabidopsis through phosphorylation of AtABF4. On the other hand, their closest ortholog RcCDPK1 in castor oil seeds is involved in the control of carbon flux by inhibitory phosphorylation. LC-MS/MS analysis revealed common phosphorylation sites in AtABF4 and its castor ortholog. Several new substrates of AtCPK4/RcCDPK1 were identified, expanding the regulatory networks associated with Ca2+ and ABA signaling, immune responses, and central carbon metabolism.
Article
Biochemistry & Molecular Biology
Jesus Bertran-Gonzalez, Caroline Dinale, Miriam Matamales
Summary: Multidisciplinary evidence suggests that the control of voluntary action is mediated by two major forms of behavioral processing: cognitively guided and autonomously guided. Brain-state irregularities, such as aging, often shift control towards the latter, but the neural mechanisms responsible are still unknown. By combining instrumental conditioning, cell-specific mapping, and chemogenetics, this study investigated strategies to enhance goal-directed control in aged mice. The findings suggest that desensitization of D2-SPN signaling in the striatum can shift behavior towards vigorous, goal-directed action and enhance cognitive functioning in habit-prone brains.
Review
Biochemistry & Molecular Biology
Stefania Battaglioni, Don Benjamin, Matthias Waelchli, Timm Maier, Michael N. Hall
Summary: The target of rapamycin (TOR), a highly conserved serine/threonine protein kinase, plays a central role in regulating cell growth and metabolism by responding to nutrients, growth factors, and cellular energy. Forming two distinct complexes, TORC1 and TORC2, TOR signaling activates cell growth by stimulating anabolic metabolism and inhibiting catabolic processes. This comprehensive review focuses on mammalian TOR (mTOR) and discusses the phosphorylation of different substrates by mTORC1 and mTORC2, despite sharing a common catalytic subunit. The conclusion is that these two complexes recruit different substrates to phosphorylate a common, minimal motif.
Article
Biochemistry & Molecular Biology
Utkarsh Nagpal, D. Kamalesh, P. Raghuraman, Sriroopreddy Ramireddy, C. Sudandiradoss
Summary: This study investigates the structural and functional abnormalities of the diabetes-related gene AS160 through molecular dynamics analysis, identifying impacts such as disrupted 14-3-3 binding and changes in phosphorylation sites caused by mutations. The research reveals that mutations increase flexibility in the region, negatively affecting stability.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Multidisciplinary Sciences
Fang Zheng, Elena Lian, Gaby Ramirez, Carley McAlister, Shuo Zhou, Wen Zhang, Chunming Liu, Rushika Perera, Chang-Guo Zhan
Summary: In this study, cytotoxicity assays were performed to examine the potential cytoprotective activity of nicotine against SARS-CoV-2 infection. The results demonstrated that nicotine had no significant cytoprotective activity against SARS-CoV-2 infection in these cells.