期刊
BIOCHEMISTRY
卷 51, 期 18, 页码 3799-3807出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi3003296
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资金
- National Institutes of Health [R01 GM092999]
- National Institutes of Health (National Cancer Institute) [Y1-CO-1020]
- National Institutes of Health (National Institute of General Medical Sciences) [Y1-GM-1104]
- United States Department of Energy, Basic Energy Sciences, Office of Science [DE-AC02-06CH11357]
DJ-1 is a conserved, disease-associated protein that protects against oxidative stress and mitochondrial damage in multiple organisms. Human DJ-1 contains a functionally essential cysteine residue (Cys106) whose oxidation is important for regulating protein function by an unknown mechanism. This residue is well-conserved in other DJ-1 homologues, including two (DJ-1 alpha and DJ-1 beta) in Drosophila melanogaster. Because D. melanogaster is a powerful model system for studying DJ-1 function, we have determined the crystal structure and impact of cysteine oxidation on Drosophila DJ-1 beta. The structure of D. melanogaster DJ-1 beta is similar to that of human DJ-1, although two important residues in the human protein, Met26 and His 126, are not conserved in DJ-1 beta His126 in human DJ-1 is substituted with a tyrosine in DJ-1 beta, and this residue is not able to compose a putative catalytic dyad with Cys106 that was proposed to be important in the human protein. The reactive cysteine in DJ-1 is oxidized readily to the cysteine-sulfinic acid in both flies and humans, and this may regulate the cytoprotective function of the protein. We show that the oxidation of this conserved cysteine residue to its sulfinate form (Cys-SO2-) results in considerable thermal stabilization of both Drosophila DJ-1 beta and human DJ-1. Therefore, protein stabilization is one potential mechanism by which cysteine oxidation may regulate DJ-1 function in vivo. More generally, most close DJ-1 homologues are likely stabilized by cysteine-sulfinic acid formation but destabilized by further oxidation, suggesting that they are biphasically regulated by oxidative modification.
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