Article
Biochemical Research Methods
Sean S. Chung, Erik J. Bidstrup, Jasmine M. Hershewe, Katherine F. Warfel, Michael C. Jewett, Matthew P. DeLisa
Summary: A method for displaying and evolving proteins with complex post-translational modifications has been developed, allowing for the efficient and site-specific modification of target proteins with different glycan structures.
ACS SYNTHETIC BIOLOGY
(2022)
Editorial Material
Medicine, General & Internal
Christine M. M. Lovly
Summary: Lung cancer is the leading cause of cancer-related death worldwide. Patients with early-stage lung cancer have lower survival rates compared to other common cancers, highlighting the need for improved systemic control methods such as targeted therapies against mutant oncoproteins and immune checkpoint inhibitors.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Review
Chemistry, Applied
Amol D. Gholap, Satish Rojekar, Harshad S. Kapare, Nikhar Vishwakarma, Sarjana Raikwar, Atul Garkal, Tejal A. Mehta, Harsh Jadhav, Mahendra Kumar Prajapati, Uday Annapure
Summary: Chitosan, a natural polysaccharide derived from chitin, holds great potential as a biomaterial in various biomedical applications due to its biocompatibility, biodegradability, antibacterial activity, and ease of modification. This review provides an overview of the use of chitosan scaffolds in tissue engineering, regenerative medicine, and drug delivery systems. It highlights the structural and biological properties of chitosan and explores different fabrication methods, such as gelation, electrospinning, and 3D printing. The review emphasizes the role of chitosan scaffolds in promoting cell adhesion, proliferation, and differentiation for tissue regeneration, as well as their potential in wound healing and infection management. Additionally, it discusses the challenges and future objectives in scaffold design, mechanical properties, and understanding interactions with host tissues.
CARBOHYDRATE POLYMERS
(2024)
Article
Genetics & Heredity
Claudia Castiglioni, Francois Feillet, Christine Barnerias, Arnaud Wiedemann, Jordi Muchart, Fanny Cortes, Cristina Hernando-Davalillo, Raquel Montero, Thierry Dupre, Arnaud Bruneel, Nathalie Seta, Sandrine Vuillaumier-Barrot, Mercedes Serrano
Summary: SSR4 is a subunit of the translocon-associated protein complex, with pathogenic variants causing CDG. Patients present with hypotonia, failure to thrive, and dysmorphic traits, emphasizing the importance of genetic testing and diagnosis.
Review
Immunology
Ashley Frazer-Abel, Michael Kirschfink, Zoltan Prohaszka
Summary: Complement not only plays a crucial role in host microbial defense but also modulates adaptive immune responses by modifying T and B cell reactivity. Dysregulation and overactivation of the complement system in clinical practice are major causes of various inflammatory and autoimmune diseases. Efforts to standardize complement testing have improved consistency across laboratories and provided a comprehensive insight into deficiencies and activation state of the complement system for better defining disease severity, evolution, and response to therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Melanie Shadrick, Keith J. Stine, Alexei V. Demchenko
Summary: This study presents a stereoselective synthesis of alpha-galactosides using galactosyl chlorides as glycosyl donors under the catalytic system of Ag2SO4 and Bi(OTf)3.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Neurosciences
Pragya Srivastava, Panagiotis Fotiadis, Linden Parkes, Dani S. Bassett
Summary: Network neuroscience has emerged as a natural framework for studying the brain, integrating graph theory and neuroscience. It has evolved to incorporate advanced graph-theoretic tools and interdisciplinary perspectives, such as machine learning and systems engineering, expanding the problem space it can address. This review explores descriptive, predictive, and perturbative network neuroscience, providing insights and future directions.
Article
Biochemistry & Molecular Biology
Shivani Yadav, Dhruvi Shah, Parmeswar Dalai, Reena Agrawal-Rajput
Summary: Antibiotics have been a lifesaver for humanity, but their efficacy has been diminished by antibiotic resistance and side effects. However, research on the non-antibacterial effects of antibiotics has provided valuable insights and potential new applications, such as their use as anticancer agents.
Article
Chemistry, Organic
Patrycja Mala, Christian Marcus Pedersen
Summary: N-Glycosyl N-sulfonyl amides were synthesized using self-promoted glycosylation without the need for catalysts or additives. The method allows for the selective beta-glycosylation of sulfonylated asparagine derivatives, providing access to glycosyl sulfonyl amides under mild conditions.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Computer Science, Artificial Intelligence
Ahmet Soylu, Oscar Corcho, Brian Elvesaeter, Carlos Badenes-Olmedo, Tom Blount, Francisco Yedro Martinez, Matej Kovacic, Matej Posinkovic, Ian Makgill, Chris Taggart, Elena Simperl, Till C. Lech, Dumitru Roman
Summary: Public procurement is a significant market that impacts various organizations and individuals, requiring governments to ensure transparency, efficiency, and healthy competition. Initiatives like open data and cross-border data integration have the potential to enhance competition and lower barriers for smaller suppliers, but existing challenges include technical heterogeneity, data quality issues, and insufficient metadata.
Article
Multidisciplinary Sciences
Adam Kuzdralinski, Marek Miskiewicz, Hubert Szczerba, Wojciech Mazurczyk, Jeff Nivala, Bogdan Ksiezopolski
Summary: The commercialization of DNA tagging demonstrates the increasing practicality of this novel approach. DNA has unique characteristics that allow it to remain stable in varying conditions and store data. DNA tagging has distinct advantages over traditional labeling techniques and has potential applications in various domains.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
James W. Dressman, Colin T. McDowell, Xiaowei Lu, Peggi M. Angel, Richard R. Drake, Anand S. Mehta
Summary: N-linked glycosylation plays a crucial role in immune response and cell-to-cell interactions. The current analytical techniques for studying immune cell N-glycosylation have limitations in throughput, structural information, starting materials, and cell purification. This study proposes a rapid antibody array-based approach coupled with MALDI-IMS for analyzing cellular N-glycosylation, which provides a valuable tool for glycoimmunology research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Physics, Particles & Fields
Abhay Ashtekar, Neev Khera, Maciej Kolanowski, Jerzy Lewandowski
Summary: This paper introduces the non-expanding horizons (NEHs) as a model for blackholes and cosmological horizons in equilibrium situations. It also discusses the multipole moments to characterize their geometry and the symmetry group of NEHs. It further explores the potential applications of NEHs in defining charges and fluxes, as well as studying perturbed NEHs and gravitational wave tomography.
JOURNAL OF HIGH ENERGY PHYSICS
(2022)
Review
Polymer Science
Junpeng Cao, Yingxin Tan, Ying Chen, Li Zhang, Jianbo Tan
Summary: This review discusses the recent advancements in polymerization-induced self-assembly (PISA), highlighting the expansion of methods including synthesis of nonlinear block copolymers, in situ synthesis of polymer blends, and utilization of macro-CTAs with low chain transfer constants. These examples provide new insights into PISA research and demonstrate the potential impact of these methods on polymer and colloid synthesis in the future.
MACROMOLECULAR RAPID COMMUNICATIONS
(2021)
Review
Orthopedics
B. Fafilek, M. Bosakova, P. Krejci
Summary: Activating mutations in the FGFR3 receptor tyrosine kinase lead to the most common genetic dwarfism in humans, achondroplasia. A stable variant of the C-natriuretic peptide, vosoritide, has been approved as a treatment for achondroplasia, and other drugs targeting FGFR3 signaling are progressing through clinical trials. This review explores current therapeutics for achondroplasia, their mechanisms, potential cures, and options for repurposing these drugs for unrelated dwarfing conditions.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Review
Biochemistry & Molecular Biology
Karen N. Allen, Sonya Entova, Leah C. Ray, Barbara Imperiali
TRENDS IN BIOCHEMICAL SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Sonya Entova, Ziqiang Guan, Barbara Imperiali
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Biochemistry & Molecular Biology
Leah Seebald, Amael G. E. Madec, Barbara Imperiali
Review
Biochemistry & Molecular Biology
Barbara Imperiali
CURRENT OPINION IN CHEMICAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Karen N. Allen, Barbara Imperiali
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Elizabeth M. Ward, Megan E. Kizer, Barbara Imperiali
Summary: Glycans have significant impacts on biological processes, disease states, and pathogenic interactions. Glycan-binding proteins, such as lectins, are crucial tools for investigating glycan structure and function, but the lack of specific and selective protein reagents hinders glycans research. Efforts have been made to expand the toolbox of GBPs beyond natural sources through protein engineering to enhance their properties and broaden their potential applications.
ACS CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Katherine H. O'Toole, Barbara Imperiali, Karen N. Allen
Summary: The monoPGT superfamily is characterized by high diversity among its members, with frequent fusion with other pathway members. Phylogenetic reconstruction reveals a burst of functionalization within the superfamily, with a minority of members containing only the minimal PGT catalytic domain. The commonality of fusion partners in the monoPGT superfamily suggests advantageous colocalization of pathway members at membrane interfaces.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Katherine H. O'Toole, Hannah M. Bernstein, Karen N. Allen, Barbara Imperiali
Summary: Phosphoglycosyl transferases (PGTs) are essential for the initiation of complex glycoconjugate biosynthesis pathways and recent research has revealed two unique superfamilies of PGT enzymes. Studies on PGTs have been challenging due to their integral membrane nature but exciting new information on their structures and mechanisms has been derived. The two superfamilies, polyPGT and monoPGT, may initiate different pathways within the same organism and have distinct modes of catalysis.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Chemistry, Organic
Christine A. Arbour, Barbara Imperiali
Summary: This article presents a method to access a library of nucleoside analogue inhibitors using solid-phase synthesis, which simplifies compound handling and purification steps.
Article
Biochemistry & Molecular Biology
Elizabeth M. Ward, Cristina Y. Zamora, Nathaniel S. Schocker, Soumi Ghosh, Megan E. Kizer, Barbara Imperiali
Summary: This study developed glycan-binding proteins (GBPs) with specificities and affinities similar to currently available lectins through directed evolution. These proteins can be used for glycoprotein blotting and cell staining, and will have significant impact on biomedical and glycobiological research.
ACS CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alyssa J. Anderson, Leah M. Seebald, Christine A. Arbour, Barbara Imperiali
Summary: This study focuses on the monoPGT enzymes that play a crucial role in the assembly of prokaryotic glycoconjugates. By using activity-based protein profiling probes, the researchers aim to understand the activity and regulation of this enzyme superfamily from a cellular proteome perspective.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alyssa J. J. Anderson, Greg J. J. Dodge, Karen N. N. Allen, Barbara Imperiali
Summary: In this study, the C-terminus of a prototypic monoPGT was structurally characterized for the first time, revealing that 19 C-terminal residues play a significant structural role in a subset of monoPGTs. This new structural information enabled the identification of co-conserved sequence fingerprints that predict NDP-sugar substrate specificity for this subset of monoPGTs. A Hidden Markov model was generated to assign the substrate of previously unannotated monoPGTs accurately.
Article
Biochemical Research Methods
Greg J. Dodge, Hannah M. Bernstein, Barbara Imperiali
Summary: Phosphoglycosyl transferases (PGTs) are membrane-bound enzymes involved in bacterial glycoconjugate biosynthesis. However, there is a lack of robust expression and purification protocols for a specific subfamily of PGTs. Recent advances in detergent-free methods for membrane protein solubilization have allowed for the direct purification of difficult membrane proteins using lip-onanoparticles. By utilizing autoinduction, SUMO tag cleavage, SMALPs, and Strep-Tag purification, a workflow for the expression and purification of previously unobtainable PGTs has been established. The generated material is highly pure and can be directly visualized using CryoEM, making these methods applicable to other membrane proteins expressed in E. coli and relevant to the membrane proteomics community.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Article
Chemistry, Organic
Christine A. Arbour, Ellen M. Fay, Joanna F. McGouran, Barbara Imperiali
Summary: A versatile solid-phase synthesis method is introduced for the diversification of thymine-containing nucleoside analogs. A library of compounds is prepared and analyzed with SNM1A, resulting in the discovery of a promising nucleoside-derived inhibitor with an IC50 of 12.3 μM.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jean-Marie Swiecicki, Jordan Tyler Santana, Barbara Imperiali
CELL CHEMICAL BIOLOGY
(2020)