Article
Biochemistry & Molecular Biology
Daniel Kachkin, Kirill V. Volkov, Julia Sopova, Alexander G. Bobylev, Sergei A. Fedotov, Sergei G. Inge-Vechtomov, Oxana Galzitskaya, Yury O. Chernoff, Aleksandr A. Rubel, Anna Y. Aksenova
Summary: This study assessed the amyloid properties of RAD51 protein in vitro and in a bacterial system. The purified human RAD51 protein formed amyloid-like aggregates with a cross-beta fibrillar structure, and were stained with amyloid-specific dyes. Cytoplasmic aggregates of RAD51 were also observed in cell cultures overexpressing RAD51.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Aoqiong Zeng, Juncai Leng, Ruijin Yang, Wei Zhao
Summary: This study developed a stable and effective iron fortification material using whey protein fibrils as nanocarriers. Compared with traditional iron fortificants, these fibrils had higher antioxidant activity and iron bioavailability, and can be widely applied in the management of iron-deficiency anemia.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ravit Malishev, Shani Ben-Zichri, Ofek Oren, Nitzan Shauloff, Tal Peretz, Ran Taube, Niv Papo, Raz Jelinek
Summary: The BIM-BH3 motif is a key pro-apoptotic domain that forms toxic amyloid fibrils, leading to cell death and impacting mitochondrial functions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Jiangtao Zhou, Ting Li, Mohammad Peydayesh, Mattia Usuelli, Viviane Lutz-Bueno, Jie Teng, Li Wang, Raffaele Mezzenga
Summary: Amyloid functional materials derived from oat globulin show diverse functionalities with rich polymorphism, reversible and irreversible fibrillization processes. These materials can be applied in water purification, sensors, and electrodes, with demonstrated sustainability against other protein sources for environmentally-efficient advanced materials and technologies.
Article
Plant Sciences
Georgia I. Nasi, Foteini D. Aktypi, Panagiotis M. Spatharas, Nikolaos N. Louros, Paraskevi L. Tsiolaki, Vassiliki Magafa, Ioannis P. Trougakos, Vassiliki A. Iconomidou
Summary: This study investigates the amyloidogenic properties of a 34-residue-long peptide in Arabidopsis thaliana under different pH conditions, and explores its association with other A. thaliana proteins using bioinformatics tools.
Article
Chemistry, Multidisciplinary
Gagandeep Singh, Manvir Kaur, Manpreet Singh, Harmandeep Kaur, Tejwant Singh Kang
Summary: This study reveals the role of time-dependent hydrolysis of [BF4](-) anion in ionic liquid [BF4] on inducing conformational changes in bovine serum albumin (BSA) and resulting in amyloid fiber formation. The addition of 10% IL in aqueous solution promotes the fibrillation of BSA most effectively among the investigated systems.
Article
Chemistry, Multidisciplinary
Manjeet Kumar, Magdalena I. Ivanova, Ayyalusamy Ramamoorthy
Summary: In patients with early pathological changes of Alzheimer's disease, Monosialoganglioside GM1-bound amyloid beta-peptides are detected. This study reveals that non-micellar GM1 can modulate A beta(40) aggregation, resulting in the formation of stable, short, rod-like, and cytotoxic A beta(40) protofibrils that can enhance the aggregation of both A beta(40) and A beta(42).
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Olga N. Makshakova, Liliya R. Bogdanova, Dzhigangir A. Faizullin, Elena A. Ermakova, Yuriy F. Zuev
Summary: This study demonstrates the potential of sulfated polysaccharides as natural assistants in restoring damaged protein structures, particularly in eliminating cross-beta structures associated with amyloid fibrils. The effects of different types of polysaccharides on protein secondary structure conversion vary depending on their conformation and charge distribution along the chain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Max O. D. G. Baker, Nirukshan Shanmugam, Chi L. L. Pham, Sarah R. Ball, Emma Sierecki, Yann Gambin, Megan Steain, Margaret Sunde
Summary: TIR-domain-containing adapter-inducing interferon-beta (TRIF) is an innate immune protein that acts as an adaptor in the context of infection. TRIF can activate the programmed cell death pathway necroptosis, and its RHIM sequence plays a role in the formation of amyloid fibrils. The interactions between RHIMs of TRIF, RIPK1, and RIPK3 are involved in the signaling of necroptosis.
Article
Chemistry, Multidisciplinary
Evelyn Rose Kamski-Hennekam, Jinfeng Huang, Rashik Ahmed, Giuseppe Melacini
Summary: The ability of ATP to modulate protein solubility is critical in understanding proteinopathies like Parkinson's disease. ATP levels decline with age, which is the most significant risk factor for Parkinson's. This study shows that ATP affects multiple stages of alpha-synuclein aggregation, and the disruption of ATP's function may play a role in Parkinson's etiology.
Review
Biochemistry & Molecular Biology
Anna Sulatskaya, Anastasiia O. Kosolapova, Alexander G. Bobylev, Mikhail Belousov, Kirill S. Antonets, Maksim Sulatsky, Irina M. Kuznetsova, Konstantin K. Turoverov, Olesya Stepanenko, Anton A. Nizhnikov
Summary: Both amyloids and beta-barrel proteins have beta-sheet-rich structures, with the latter being able to form functional amyloids in vivo. These beta-barrel amyloid proteins can interact with each other and form toxic oligomers, potentially contributing to the development of amyloidoses. Rapidly growing discoveries suggest that the number and diversity of functions of amyloid-forming beta-barrel proteins are significantly greater than currently understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yuying Fu, Yuan Li, Huanhuan Su, Tao Wu, Teng Li
Summary: Ice recrystallization, harmful to frozen foods and cellular cryopreservation, can be prevented by biocompatible materials with ice recrystallization inhibition (IRI) activity. Amphiphilic amyloid protein fibrils (APFs) prepared from whey protein isolate (WPI) have shown IRI activity. The IRI activity of APFs is influenced by pH, water dispersibility, fibril length, and NaCl content. APFs bind weakly to ice and ordered water may play a role in their IRI activity. These findings suggest the potential use of APFs as novel ice recrystallization inhibitors.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Zejian Xu, Guancheng Shan, Nairong Hao, Lianwei Li, Tian Lan, Yabo Dong, Jiayu Wen, Ran Tian, Yan Zhang, Lianzhou Jiang, Xiaonan Sui
Summary: This study presents a strategy to tailor soy protein-derived amyloid fibrils (SAFs) into hydrogels using (-)-epigallocatechin-3-gallate (EGCG). EGCG can cleave SAFs and induce the formation of branched chains, leading to gel-like structures. SAFs-EGCG complexes show superior antibacterial activity and are non-toxic to human cells.
Article
Nanoscience & Nanotechnology
Zhengxun Wei, Siqing Dai, Jiayi Huang, Xinyu Hu, Chengxin Ge, Ximing Zhang, Kai Yang, Ping Shao, Peilong Sun, Ning Xiang
Summary: The research focuses on the development of sustainable and edible scaffolds using soy protein amyloid fibrils for cultivated meat production. The scaffolds were crosslinked using microbial transglutaminase and temperature-controlled water vapor annealing, resulting in 3D porous scaffolds. These soy protein amyloid fibril scaffolds showed potential for use in the food industry as they had suitable pore sizes, porosities, and compressive moduli, and could degrade within a short time.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Applied
Yuan Peng, Chuanyang Wang, Jian Yu, Jinhong Wu, Faxiang Wang, Yongle Liu, Xianghong Li
Summary: This study investigated the intermolecular interactions and self-assembly mechanism of rice glutelin amyloid fibril aggregates using experimental and computer simulation methods. The addition of NaCl and SDS promoted the aggregation of the fibrils, while the addition of urea caused degradation and depolymerization. Hydrogen bonding primarily drove the formation of the common cross 8-sheet structure, with hydrophobic and electrostatic interactions also involved. Molecular dynamics simulations confirmed the nucleation-elongation mechanism for the formation of the aggregates. Understanding the self-assembly pattern of amyloid fibril aggregates derived from food protein is crucial for their design and practical application.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Multidisciplinary
Andrew J. Christofferson, Zahraa S. Al-Garawi, Nevena Todorova, Jack Turner, Mark P. Del Borgo, Louise C. Serpell, Marie-Isabel Aguilar, Irene Yarovsky
Article
Biochemistry & Molecular Biology
Karen E. Marshall, Devkee M. Vadukul, Kevin Staras, Louise C. Serpell
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Saskia J. Pollack, Jasmine Trigg, Tahmida Khanom, Luca Biasetti, Karen E. Marshall, Youssra K. Al-Hilaly, Janet E. Rickard, Charles R. Harrington, Claude M. Wischik, Louise C. Serpell
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Mahmoud B. Maina, Gunasekhar Burra, Youssra K. Al-Hilaly, Kurtis Mengham, Kate Fennell, Louise C. Serpell
Article
Chemistry, Multidisciplinary
Liam D. Aubrey, Ben J. F. Blakeman, Liisa Lutter, Christopher J. Serpell, Mick F. Tuite, Louise C. Serpell, Wei-Feng Xue
COMMUNICATIONS CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Gunasekhar Burra, Mahmoud B. Maina, Louise C. Serpell, Ashwani K. Thakur
Summary: This study used a reproducible solubilisation protocol to generate a homogenous monomeric solution of the yeast prion protein Sup35 N-terminal hepta-peptide sequence GNNQQNY, revealing molecular details of its self-assembly mechanism. The aggregation kinetics data showed that the GNNQQNY sequence follows nucleation-dependent aggregation kinetics with a critical nucleus of size similar to 7 monomers, where the efficiency of nucleation was inversely related to the reaction temperature.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Jack Devonport, Nikolett Bodnar, Andrew McGown, Mahmoud Bukar Maina, Louise C. Serpell, Csilla Kallay, John Spencer, George E. Kostakis
Summary: Salpyran is a tunable Cu(II) chelating scaffold with high affinity and selectivity, showing potential therapeutic application.
INORGANIC CHEMISTRY
(2021)
Article
Neurosciences
Luca Biasetti, Stephanie Rey, Milena Fowler, Arjuna Ratnayaka, Kate Fennell, Catherine Smith, Karen Marshall, Catherine Hall, Mariana Vargas-Caballero, Louise Serpell, Kevin Staras
Summary: Alzheimer's disease is associated with increased levels of A beta in the brain, and this study investigates the impact of A beta on functional presynaptic vesicle pools. The researchers found that A beta treatment led to an enlargement of functional vesicles and an accumulation of recycled vesicles near endocytic sites. This resulted in deficits in vesicle retrieval pathways and limited information signaling capacity. Treatment with an antiepileptic drug partially rescued these transmission defects.
Review
Neurosciences
Oliver George Steele, Alexander Cameron Stuart, Lucy Minkley, Kira Shaw, Orla Bonnar, Silvia Anderle, Andrew Charles Penn, Jennifer Rusted, Louise Serpell, Catherine Hall, Sarah King
Summary: The APOE gene is the most significant genetic risk factor for late-onset Alzheimer's disease, with the APOE4 genotype being associated with neuropathological and cognitive deficits. It has been proposed that APOE4 leads to cerebral dysfunction through various mechanisms, including neurodegeneration, neurovascular dysfunction, neuroinflammation, and oxidative stress. Further study is needed to understand the complex interplay between APOE4 and these mechanisms and their impact on brain function.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Kurtis Mengham, Youssra Al-Hilaly, Sebastian Oakley, Kamillia Kasbi, Mahmoud B. Maina, Louise C. Serpell
Summary: Tau is a protein that can form different fibers in Alzheimer's disease and other taupathies. It can bind to microtubules and has the ability of phase separation. The various conformations of this protein are crucial for its biological function and its association with neurodegenerative diseases.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Gabrielle Audsley, Harry Carpenter, Nsikak B. Essien, James Lai-Morrice, Youssra Al-Hilaly, Louise C. Serpell, Geoffrey R. Akien, Graham J. Tizzard, Simon J. Coles, Cristina Pubill Ulldemolins, George E. Kostakis
Summary: Two propeller-shaped chiral CoIII3YIII complexes synthesized using fluorinated ligands were characterized by various techniques. These complexes were found to have sensing and discrimination abilities, which allowed for the development of an innovative 19F NMR chemosensory system. Control experiments and theoretical studies provided insights into the sensing mechanism, and the limitations of this method were discussed.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Rui Huang, Daniel McDowall, Henry Ng, Lisa Thomson, Youssra K. Al-Hilaly, James Doutch, Sam Burholt, Louise C. Serpell, Robert J. Poole, Dave J. Adams
Summary: A functionalized dipeptide that self-assembles into wormlike micelles at high pH can act as a surfactant, and by varying salt concentration, the self-assembled structures and interactions can be changed, resulting in solutions with different shear and extensional viscosity. Gel noodles with different mechanical properties can be prepared from these solutions.
CHEMICAL COMMUNICATIONS
(2022)
Review
Chemistry, Multidisciplinary
Pu Chun Ke, Ruhong Zhou, Louise C. Serpell, Roland Riek, Tuomas P. J. Knowles, Hilal A. Lashuel, Ehud Gazit, Ian W. Hamley, Thomas P. Davis, Marcus Fandrich, Daniel Erik Otzen, Matthew R. Chapman, Christopher M. Dobson, David S. Eisenberg, Raffaele Mezzenga
CHEMICAL SOCIETY REVIEWS
(2020)
Article
Chemistry, Inorganic & Nuclear
Stavroula Sampani, Youssra K. Al-Hilaly, Sharali Malik, Louise C. Serpell, George E. Kostakis
DALTON TRANSACTIONS
(2019)
Article
Chemistry, Multidisciplinary
Kate McAulay, Bart Dietrich, Hao Su, Michael T. Scott, Sarah Rogers, Youssra K. Al-Hilaly, Honggang Cui, Louise C. Serpell, Annela M. Seddon, Emily R. Draper, Dave J. Adams