Article
Oncology
Lanyang Gao, Danli Zhu, Qin Wang, Zheng Bao, Shigang Yin, Huiyan Qiang, Heinrich Wieland, Jinyue Zhang, Alexander Teichmann, Jing Jia
Summary: USP7 is overexpressed in melanoma and affects specific proteins and processes through multiple signaling pathways, with knockdown of USP7 slowing down melanoma cell proliferation.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Patricia R. Nano, Taylor K. Johnson, Takamasa Kudo, Nancie A. Mooney, Jun Ni, Janos Demeter, Peter K. Jackson, James K. Chen
Summary: ARHGAP36 is an atypical member of the Rho GTPase-activating protein (GAP) family, with multiple functional domains and regulatory modes. It influences spinal cord development and tumorigenesis by regulating Gli transcription factor activation and interaction with other proteins.
Article
Biochemistry & Molecular Biology
Pengjiao Hou, Wei Hao, Bo Qin, Mengyun Li, Rong Zhao, Sheng Cui
Summary: Schlafen11 is a well-studied protein that plays important roles in cancer therapy and virus-host interactions. The crystal structure and biochemical characteristics of the N-terminal domain (NTD) of Schlafen11 were determined, revealing its potent RNase activity towards type I and II tRNAs and rRNAs. These findings enhance our understanding of the Schlafen family.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
John J. W. Han, Daniel Ho, Hyun M. Kim, Jun Y. Lee, Yerin S. Jeon, Jefferson Y. Chan
Summary: The study identified ubiquitin specific protease 7 (USP7) as a novel regulator of Nrf1 activity, modulating the stability and gene expression levels of Nrf1 protein by regulating its ubiquitination. Loss of USP7 function attenuated the induction of Nrf1 protein expression in response to toxic metal exposure.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sara T. Haile, Sanim Rahman, James K. Fields, Benjamin C. Orsburn, Namandje N. Bumpus, Cynthia Wolberger
Summary: The SAGA complex, which includes the HAT module, is a transcriptional co-activator that modifies histones through acetylation and deubiquitination. The SWIRM domain plays a crucial role in incorporating the HAT module into the SAGA complex. Deletion of the SWIRM domain results in loss of the HAT module and decreases the deubiquitinating activity of SAGA.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Article
Biochemistry & Molecular Biology
Manuel Lisetto, Mariagiulia Fattorini, Andrea Lanza, Marco Gerdol, Martin Griffin, Zhuo Wang, Fortunato Ferrara, Daniele Sblattero
Summary: Transglutaminase 2 (TG2) is a multifunctional protein involved in various physiological and pathological processes. Its role in these processes is controversial due to different effects depending on localization, cell type, or experimental conditions. This study characterized the enzymatic and functional properties of TG2 proteins in zebrafish and demonstrated their similarities to the human ortholog.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anna Ciarkowska, Maciej Ostrowski, Anna Kozakiewicz
Summary: The research reported the biochemical characteristics of recombinant maize IAGlc synthase, highlighting its ability to alter plant hormonal homeostasis; the enzyme shows substrate specificity towards IAA but can also glucosylate other substrates. Additionally, ATP and glucose-1-phosphate exhibit dual effects on the enzyme's activity, acting as activators at low concentrations and inhibitors at higher concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Camila A. Cotrim, Russell J. Jarrott, Andrew E. Whitten, Hassanul G. Choudhury, David Drew, Jennifer L. Martin
Summary: In this study, it was demonstrated that human ZnT1 and its variant can be expressed and purified in yeast cells, with the purified hZnT1 variant showing Zn2+/H+ antiporter activity. Additionally, the soluble C-terminal domain of hZnT1 was successfully expressed, purified, and characterized in a bacterial expression system, showing an increase in melting temperature at acidic pH and forming a dimer in solution based on small-angle X-ray scattering analysis.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Fengwu Zhang, Yongchao Zhao, Yi Sun
Summary: USP2 was identified as a new deubiquitylase that stabilizes SKP2 protein by disrupting its interaction with substrates, thereby affecting cell growth regulation. Inactivation of USP2 can shorten SKP2 protein half-life and enhance its ubiquitylation and degradation, but surprisingly, USP2-stabilized SKP2 does not destabilize its substrates p21 and p27. Knockdown or inhibition of USP2 can partially mediate growth suppression through modulation of SKP2 and its substrates.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Sang Chul Shin, Jinyoung Park, Kyung Hee Kim, Jung Min Yoon, Jinhong Cho, Byung Hak Ha, Yeonji Oh, Hyunah Choo, Eun Joo Song, Eunice EunKyeong Kim
Summary: This study investigates the functional and biochemical properties of USP47, and reveals that depleting USP47 inhibits cancer cell growth in a p53-dependent manner and that full-length USP47 has higher deubiquitinase activity. Additionally, the study provides molecular details of USP47 regulation, substrate recognition, and potential hotspots for drug discovery.
COMMUNICATIONS BIOLOGY
(2023)
Article
Plant Sciences
Ridhi Khurana, Sanchi Bhimrajka, Gundra Sivakrishna Rao, Vibha Verma, Neelima Boora, Gautam Gawande, Meenu Kapoor, Khareedu Venkateswara Rao, Sanjay Kapoor
Summary: OsMADS29 is a seed-specific transcription factor involved in embryo and endosperm development and starch filling during seed development in rice. The upstream regulatory region of OsMADS29 contains both positive and negative regulatory elements, as well as auxin-responsive elements, which guide its development-dependent and spatial expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Xinning Liu, Yuanchang Liu, Guangjian Zhao, Yidan Zhang, Lu Liu, Juan Wang, Yifan Wang, Siyu Zhang, Xin Li, Dongliang Guo, Peng Wang, Ximing Xu
Summary: This study investigated the impact of NAT from Vibrio vulnificus on acetyl group transformation in arylamine antibiotics, focusing on its structure, enzyme activity, and stability, revealing its activity under different conditions and sensitivity to metal ions. Additionally, kinetic parameters and structural analysis showed that (VIBVN)NAT could acetylate various aromatic amine substrates and contribute to arylamine antibiotic resistance in Vibrio vulnificus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniele Santorelli, Lucia Marcocci, Valeria Pennacchietti, Caterina Nardella, Awa Diop, Paola Pietrangeli, Livia Pagano, Angelo Toto, Francesca Malagrino, Stefano Gianni
Summary: Cooperativity is a well-established property of folding, but our understanding of this feature in multidomain folding is limited. This study compares the folding of a tandem repeat protein with two PDZ domains to isolated PDZ domains. The results show that while each PDZ domain can fold independently, the full-length construct folds as a single cooperative unit. The analysis provides insights into the molecular basis of cooperativity in multidomain systems.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Seok Won Jang, Jung Min Kim
Summary: The study found that Asp-199 plays a crucial role in the activity of USP1. The D199A mutation renders USP1 inactive, does not affect the interaction with Uaf1, but impacts the deubiquitination activity of FANCD2 and PCNA.
Review
Biochemistry & Molecular Biology
Muhammad Sajid Hamid Akash, Sumbal Rasheed, Kanwal Rehman, Muhammad Ibrahim, Muhammad Imran, Mohammed A. Assiri
Summary: Krupple-Like family of transcription factor-14 (KLF14) is a crucial gene involved in various biological functions and pathological mechanisms. It controls the expression of multiple genes and influences lipid metabolism, glucose regulation, insulin sensitivity, as well as cell apoptosis, proliferation, and differentiation. KLF14 has also been used as an epigenetic biomarker for estimating chronological age. Genetic variations in KLF14, especially in adipose tissues, are associated with dyslipidemia, insulin resistance, and glucose intolerance. The prevalence of these genetic polymorphisms varies among different populations due to ethnic differences and epigenetic modifications, and they can be influenced by environmental and physiological factors such as diet, age, gender, and obesity.