Article
Chemistry, Multidisciplinary
David N. Philpott, Surath Gomis, Hansen Wang, Randy Atwal, Abdellali Kelil, Tanja Sack, Brandon Morningstar, Chris Burnie, Edward H. Sargent, Stephane Angers, Sachdev Sidhu, Shana O. Kelley
Summary: The rapid on-cell phage display platform presented in this study accelerates the development of high-performance human antibodies by mimicking the complex in vivo binding environment, improving screening efficiency and accuracy.
ACS CENTRAL SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Maria P. Meivita, Denise Lee, J. Shamita Naikar, Shao-Xiang Go, Wey Chyi Teoh, Yaw Sing Tan, Natasa Bajalovic, Desmond K. Loke
Summary: Promising results have been shown in clinical studies using electrothermal agents (ETAs) in cancer therapy. However, a challenge lies in maintaining the balance between facilitating the degradation of ETAs and increasing electrothermal performance/stability. This study successfully controlled the thermal signature of MoS2 by utilizing MoS2 nanostructures with M13 phage (MNM) and developed a framework for cancer cell ablation and electrothermal therapy. The results demonstrated a decrease in cell viability, degradation time, and stimulus length, and molecular dynamics simulations provided insights into the structural origin of enhanced thermal conduction.
Article
Microbiology
Chenglong Ma, Chunyang Jiang, Dongping Zhao, Shuhao Li, Ronggui Li, Lei Li
Summary: Directed evolution is a widely-used engineering strategy for improving protein stability or biochemical functions through repeated rounds of mutation and selection. Using ELISA to check the displayed protein against the target and enhancing the signal through various approaches can improve the sensitivity of ELISA experiments when dealing with low-affinity ligand-protein interactions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Junxia Wang, Liang Li, Yingkun Zhang, Kaifeng Zhao, Xiaofeng Chen, Haicong Shen, Yuanqiang Chen, Jia Song, Yuqiang Ma, Chaoyong Yang, Hongming Ding, Zhi Zhu
Summary: Efficient molecular selection is crucial for various biomedical applications. By utilizing deterministic lateral-displacement microfluidics, the equilibrium of dissociation can be greatly shifted, leading to improved selection efficiency and better discrimination of ligand affinity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Radoslaw W. Piast, Rafal M. Wieczorek, Nicola Marzec, Maciej Garstka, Aleksandra Misicka
Summary: Chondrocalcinosis is a metabolic disease caused by calcium pyrophosphate dihydrate crystals in the synovial fluid. The study aimed to investigate whether short peptides could dissolve these crystals, with one peptide named R25 showing hydrolytic activity towards the crystals, dependent on Mg2+ and working against soluble pyrophosphate species.
Article
Virology
Marwa Arbi, Imen Larbi, Jihen Nsiri, Imen El Behi, Ahmed Rejeb, Khaled Miled, Adeljelil Ghram, Mehdi Houimel
Summary: This study identified new antiviral peptides that can inhibit the infectivity of H9N2 virus by screening a random linear hexapeptide library. Sixteen different phage-peptides were found to specifically bind to H9N2 virus, and two selected peptides (LSRMPK and FAPRWR) showed antiviral activity in both in ovo and in vivo experiments. These peptides prevent infection by inhibiting the attachment of the H9N2 virus to the cellular receptor.
Review
Chemistry, Multidisciplinary
Golnaz Kamalinia, Brian J. Grindel, Terry T. Takahashi, Steven W. Millward, Richard W. Roberts
Summary: mRNA display is a powerful platform for directed evolution of proteins and peptides, linking phenotype and genotype through puromycin. Iterative selection cycles lead to peptides with desired functional levels. This review discusses the development and broad application of mRNA display technology in selecting novel peptides and proteins.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
H. Hemanth, Sairam S. Mallajosyula
Summary: This study used polarizable molecular dynamics simulation to investigate the self-assembly of nucleobases on a graphene sheet. The introduction of polarization led to significant changes in interaction patterns, including the stacking probability in guanine-rich systems and the formation of hydrogen-bonded structures on the graphene sheet, highlighting the importance of DNA sequence and composition.
Article
Nanoscience & Nanotechnology
Abolfazl Alizadeh Sahraei, Barbara Mejia Bohorquez, Denise Tremblay, Sylvain Moineau, Alain Garnier, Faical Larachi, Patrick Lague
Summary: This study aimed to evaluate the interactions of peptides with quartz as potential flotation collectors, in order to find biofriendly alternatives to biohazardous chemical reagents in mining practices. The results showed that the flexibility of peptide chains directly affected their adsorption behavior. Overall, this study is of great significance for the rational design of peptide sequences for mineral processing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Pharmacology & Pharmacy
Ali Akbar Alizadeh, Michael B. Morris, W. Bret Church, Shadi Yaqoubi, Siavoush Dastmalchi
Summary: TNFa plays a crucial role in physiological processes, but excessive levels can contribute to inflammatory and autoimmune diseases. Therefore, researchers have focused on developing strategies to counteract aberrant TNFa concentrations. Promising approaches include destabilizing the active form of TNFa and blocking its interaction with TNFa receptor type I. The use of phage-display technology has been successful in identifying peptides and antibodies that inhibit TNFa activity or prevent its binding to its receptor.
DRUG DISCOVERY TODAY
(2022)
Review
Chemistry, Analytical
Vanessa Escobar, Natale Scaramozzino, Jasmina Vidic, Arnaud Buhot, Raphael Mathey, Carole Chaix, Yanxia Hou
Summary: Foodborne pathogens cause a significant number of illnesses worldwide. In recent decades, there has been a growing development of accurate biosensors for detecting bacterial pathogens in food, using peptides as recognition biomolecules. This review discusses strategies for designing and screening sensitive peptide bioreceptors, as well as advanced techniques for peptide-based biosensors and innovative approaches like electronic noses for food monitoring. These biosensors and electronic noses offer promising alternatives for highly sensitive, cost-effective, and rapid pathogen detection, some of which can be used as portable devices for on-site analysis.
Article
Multidisciplinary Sciences
Makoto Nakakido, Naoki Tanaka, Ayako Shimojo, Nobuhiro Miyamae, Kouhei Tsumoto
Summary: Respiratory infectious diseases pose a serious global threat, and novel antiviral materials are in high demand. In this study, a new platform was constructed to evaluate antiviral activity of surface coating materials using the M13 bacteriophage and phagemid system. The experimental system developed in this study provides rapid and effective screening, which can be applied to the development of novel antiviral materials.
Article
Nanoscience & Nanotechnology
Alexander J. Winton, Mark A. Allen
Summary: Phage display is used to identify polypeptides binding to lithium-ion battery materials. Output/input assays and transmission electron microscopy are employed for quantitative measure and verification. Isothermal titration calorimetry and alanine scanning determine binding residues and affinity, and a bifunctional peptide is validated for stability and potential application in lithium-ion battery electrodes.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Kangran Zhang, Yezhong Tang, Qin Chen, Yang Liu
Summary: This review discusses the regular process of using phage display technology to screen therapeutic peptides and the peptides that have been screened for their anti-inflammatory properties in recent years. The article describes how these peptides were screened and their in vitro and in vivo effects. It also explores the current challenges and future outlook of using phage display to obtain anti-inflammatory therapeutic peptides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Laura Franz, Jesko Koehnke
Summary: This study demonstrates a Sortase A-based approach for modifying peptides with enzymes from unrelated pathways, leading to the synthesis of a new-to-nature, hybrid ribosomal natural product in a one-pot reaction.
CHEMICAL COMMUNICATIONS
(2021)
