期刊
BIOCHEMISTRY
卷 49, 期 4, 页码 656-668出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi901459c
关键词
-
资金
- National Natural Science Foundation of China
- 973 Project
- Shanghai Municipal Science and Technology Commission [07DZ22940]
- Shanghai Municipal Wildlife Administration [SBHZ2006_01]
- Institut Curie
- Centre National de la Recherche Scientifique (CNRS)
Bloom's syndrome (BS) is a rare human autosomal recessive disorder characterized by a strong predisposition to a wide range of cancers commonly affecting the general population. Understanding the functioning mechanism of the BLM protein may provide the opportunity to develop new effective therapy strategies. In this work, we studied the DNA unwinding kinetic mechanism of the helicase core of the BLM protein using various stopped-now assays. We show that the helicase core of BLM unwinds duplex DNA as monomers even under conditions strongly favoring oligomerization. An unwinding rate of similar to 20 steps per second and a step size of 1 bp have been determined. We have observed that the helicase has a very low processivity. From dissociation and inhibition experiments, we have found that during its ATP hydrolysis cycle in DNA unwinding the helicase tends to dissociate from the DNA substrate in the ADP state. The experimental results imply that the BLM helicase core may unwind duplex DNA in an inchworm manner.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据