Review
Biochemistry & Molecular Biology
Ajanta Chatterji, Rajib Sengupta
Summary: Nitric oxide is a gasotransmitter molecule that plays various roles in cellular processes. Recent research supports the existence of S-nitrosylated proteins within cells, challenging the controversy over their stability. Evidence suggests that these proteins directly regulate functionality and provide insights into cell signaling.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yayoi Kimura, Yusuke Nakai, Jihye Shin, Miyui Hara, Yuriko Takeda, Sousuke Kubo, Sundararaj Stanleyraj Jeremiah, Yoko Ino, Tomoko Akiyama, Kayano Moriyama, Kazuya Sakai, Ryo Saji, Mototsugu Nishii, Hideya Kitamura, Kota Murohashi, Kouji Yamamoto, Takeshi Kaneko, Ichiro Takeuchi, Eri Hagiwara, Takashi Ogura, Hideki Hasegawa, Tomohiko Tamura, Takeharu Yamanaka, Akihide Ryo
Summary: The COVID-19 pandemic poses a significant threat to global health, and accurately predicting disease progression is crucial for determining the best treatment options. Using proteomic approaches, researchers have identified potential serum proteins associated with COVID-19 prognosis. The findings suggest that CHI3L1 and IGFALS could serve as highly sensitive prognostic markers, aiding in the diagnosis and treatment of severe COVID-19 cases.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Anthony H. Taylor, Justin C. Konje, Thangesweran Ayakannu
Summary: The number of women diagnosed and dying from endometrial cancer continues to rise globally. This study identified new protein markers specific to endometrial cancer, which can improve the accuracy of diagnosis and provide potential targets for future treatment and research.
Article
Biochemistry & Molecular Biology
Esha Sircar, Sristi Raj Rai, Mark A. Wilson, Michael G. Schlossmacher, Rajib Sengupta
Summary: Parkinson's disease is a rapidly growing neurodegenerative disorder with a strong genetic component. Aberrant S-nitrosylation of proteins like Parkin, DJ-1, and PINK1 may contribute to the pathogenesis of PD, emphasizing the importance of this post-translational modification in neuronal health.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemical Research Methods
Zi Gao, Preston Williams, Lin Li, Yinsheng Wang
Summary: DNA sequences with high G content have the potential to form G quadruplex structures, which can interact with specific proteins. Through quantitative proteomic experiments, a number of candidate G4-interacting proteins were identified, some of them being discovered for the first time. These findings highlight the complexity and importance of understanding the G4-interaction proteome in biology.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Kenji Egashira, Yoko Ino, Yusuke Nakai, Takashi Ohira, Tomoko Akiyama, Kayano Moriyama, Yu Yamamoto, Mitsuo Kimura, Akihide Ryo, Tomoyuki Saito, Yutaka Inaba, Hisashi Hirano, Ken Kumagai, Yayoi Kimura
Summary: A high-sensitivity analysis system for mouse bone proteins was established using data-independent acquisition mass spectrometry. 40 proteins showing differential abundance in a microgravity environment were detected, as well as 22 proteins maintaining similar abundance under mastication stimuli. The mechanical stimulus-responsive protein SPARCL1 was suggested to be involved in bone remodeling. These findings contribute to understanding the mechanism of bone metabolism induced by mechanical stimuli.
JOURNAL OF PROTEOMICS
(2023)
Article
Agriculture, Multidisciplinary
Daniel A. Kusza, Roger Hunter, Georgia Schafer, Muneerah Smith, Arieh A. Katz, Catherine H. Kaschula
Summary: Garlic is a medicinal plant and spice with health-promoting effects. This study designed a biotin-ajoene probe to identify the protein targets of ajoene in breast cancer cells and discovered the mechanism and pathways associated with its anticancer activity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Obstetrics & Gynecology
Yingting Li, Liuying Zhong, Cheuk-Lun Lee, Philip C. N. Chiu, Min Chen
Summary: This study identified several proteins S-nitrosylated by ADM in JEG-3 placental cells, including tubulin, enolase, eukaryotic translation initiation factor 4A1, actin, ANX II, and glyceraldehyde 3-phosphate dehydrogenaseprotein-1. ADM significantly increased the surface expression of ANX II, but not its total expression, in JEG-3 cells, which could potentially impact trophoblast invasion and migration. Future studies are needed to further understand the roles of S-nitrosylated ANX II in trophoblast functions.
REPRODUCTIVE SCIENCES
(2022)
Article
Food Science & Technology
Qiongniu Zhu, Lujuan Xing, Qin Hou, Rui Liu, Wangang Zhang
Summary: This study identified a higher abundance of S-nitrosylated proteins in beef with high ultimate pH, containing a large number of cysteine sites. Proteins related to energy metabolism enzymes and mitochondrial dysfunction were up-regulated in beef with high ultimate pH compared to those involved in calcium homeostasis.
Article
Chemistry, Physical
Haydar Taylan Turan, Markus Meuwly
Summary: The study investigated the structural dynamics and vibrational spectroscopy of S-nitrosylation in proteins, showing that S-nitrosylation can alter protein function and exhibit distinct characteristics from other protein modes. The findings of stable cis- and trans-MbSNO in agreement with experimental results on other proteins, and the recruitment of more water molecules by MbSNO suggest that nitrosylation can significantly modulate local hydration.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Biology
Yung-Chih Chen, Meaghan Smith, Ya-Lan Ying, Manousos Makridakis, Jonathan Noonan, Peter Kanellakis, Alin Rai, Agus Salim, Andrew Murphy, Alex Bobik, Antonia Vlahou, David W. Greening, Karlheinz Peter
Summary: Protein signatures of unstable and stable atherosclerosis are defined by quantitative proteomics using a preclinical mouse model of plaque instability/rupture. Key proteins and pathways, such as the innate immune system and neutrophil degranulation, are identified. Inhibition of calprotectin S100A8/A9, a validated protein signature in unstable plaques, demonstrates plaque-stabilizing effects.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemical Research Methods
Xiu-Fen Cheng, Nan Wang, Zhongyao Jiang, Zhenzhen Chen, Yaxin Niu, Lili Tong, Ting Yu, Bo Tang
Summary: In this study, four Au(I) complexes were screened and their target proteins were identified using an activity-based protein profiling strategy. The results showed that the target proteins of these complexes were mostly associated with cancer. Additionally, novel Au(I)-binding proteins and biological gold-protein interaction pathways were discovered.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhen Wu, Yi Shen, Xumin Zhang
Summary: The study establishes a novel quantitative method, TAG-TMTpro, which triples the multiplexing capacity of TMTpro by introducing Ala or Gly residues to peptides prior to labeling. The method is validated for identification and quantification performance using E. coli and HeLa cell lysates.
ANALYTICAL CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Asgar Ali, Ejaj Ahmad, Renu Verma, Parameswar Sahu, Nimisha, Siddharth Srivastava, Sundeep Singh Saluja
Summary: Gallbladder cancer is a common cancer diagnosed at a late stage, causing significant loss of human lives. By using a multi-omics approach, changes in every molecule produced in cancer cells can be identified, leading to enhanced early diagnosis and therapeutic targeting. The analysis of public multi-omics data is a valuable resource to advance research in this area. This approach has been applied to sub-categorize tumors and identify suitable therapeutic targets for gallbladder cancer patients.
BRIEFINGS IN FUNCTIONAL GENOMICS
(2023)
Article
Veterinary Sciences
Baoyu Jia, Decai Xiang, Qingyong Shao, Qionghua Hong, Guobo Quan, Guoquan Wu
Summary: A novel quantitative approach was used to compare the proteomic profiles of porcine immature and in vitro mature oocytes. The results revealed significant differences in protein expression, indicating multiple regulatory mechanisms involved in oocyte nuclear and cytoplasmic maturation.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Ziv Kaner, Rotem Engelman, Ronen Schuster, Peleg Rider, David Greenberg, Yossef Av-Gay, Moran Benhar, Eli C. Lewis
FRONTIERS IN IMMUNOLOGY
(2019)
Review
Cardiac & Cardiovascular Systems
Richard T. Premont, James D. Reynolds, Rongli Zhang, Jonathan S. Stamler
CIRCULATION RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Divya Seth, Alfred Hausladen, Jonathan S. Stamler
ANTIOXIDANTS & REDOX SIGNALING
(2020)
Correction
Cell Biology
Jasmina Zivanovic, Emilia Kouroussis, Joshua B. Kohl, Bikash Adhikari, Biljana Bursac, Sonia Schott-Roux, Dunja Petrovic, Jan Lj. Miljkovic, Daniel Thomas-Lopez, Youngeun Jung, Marko Miler, Sarah Mitchell, Verica Milosevic, Jose Eduardo Gomes, Moran Benhar, Bruno Gonzalez-Zorn, Ivana Ivanovic-Burmazovic, Roberta Torregrossa, James R. Mitchell, Matthew Whiteman, Guenter Schwarz, Solomon H. Snyder, Bindu D. Paul, Kate S. Carroll, Milos R. Filipovic
Article
Biochemistry & Molecular Biology
Ilana Braunstein, Rotem Engelman, Ofer Yitzhaki, Tamar Ziv, Erwan Galardon, Moran Benhar
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Review
Biochemistry & Molecular Biology
Moran Benhar
Article
Biochemistry & Molecular Biology
Richard T. Premont, James D. Reynolds, Rongli Zhang, Jonathan S. Stamler
Summary: Red blood cell-mediated vasodilation is crucial for oxygen delivery, facilitated by the conversion of heme-bound nitric oxide into vasodilator S-nitrosothiol. This process is regulated by a strictly conserved Cys residue in hemoglobin. The model of a three-gas respiratory cycle (O-2/NO/CO2) is supported by the coupling of hemoglobin allostery to NO conversion and SNO release.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Min-Kyoo Shin, Edwin Vazquez-Rosa, Yeojung Koh, Matasha Dhar, Kalyani Chaubey, Coral J. Cintron-Perez, Sarah Barker, Emiko Miller, Kathryn Franke, Maria F. Noterman, Divya Seth, Rachael S. Allen, Cara T. Motz, Sriganesh Ramachandra Rao, Lara A. Skelton, Machelle T. Pardue, Steven J. Fliesler, Chao Wang, Tara E. Tracy, Li Gan, Daniel J. Liebl, Jude P. J. Savarraj, Glenda L. Torres, Hilda Ahnstedt, Louise D. McCullough, Ryan S. Kitagawa, H. Alex Choi, Pengyue Zhang, Yuan Hou, Chien-Wei Chiang, Lang Li, Francisco Ortiz, Jessica A. Kilgore, Noelle S. Williams, Victoria C. Whitehair, Tamar Gefen, Margaret E. Flanagan, Jonathan S. Stamler, Mukesh K. Jain, Allison Kraus, Feixiong Cheng, James D. Reynolds, Andrew A. Pieper
Summary: Traumatic brain injury induces tau acetylation, leading to neurodegeneration and neurobehavioral impairment. Protecting mice from TBI can be achieved by blocking GAPDH S-nitrosylation, inhibiting p300/CBP, or stimulating Sirtuin1. Ac-tau may serve as a therapeutic target and potential blood biomarker of TBI.
Review
Endocrinology & Metabolism
Hua-Lin Zhou, Richard T. Premont, Jonathan S. Stamler
Summary: This review discusses the crucial role of S-nitrosylation in the regulation of insulin signaling throughout the insulin life cycle, impacting both metabolic health and disease. The interplay between insulin signaling and protein S-nitrosylation is highlighted, with aberrant S-nitrosylation contributing to metabolic disorders such as type 2 diabetes mellitus. Understanding the impact of S-nitrosylation on cellular metabolism could provide valuable insights in the field of metabolic signaling and insulin-related disorders.
NATURE REVIEWS ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Megan Pophal, Zachary W. Grimmett, Clara Chu, Seunghee Margevicius, Thomas Raffay, Kristie Ross, Anjum Jafri, Olivia Giddings, Jonathan S. Stamler, Benjamin Gaston, James D. Reynolds
Summary: The study suggests that F-ENO could reflect airway S-nitrosothiol concentrations and serve as a biomarker for asthma. Inhalational challenge with GSNO resulted in increased F-ENO, indicating a potential non-invasive test for monitoring airway GSNO breakdown.
Article
Biochemistry & Molecular Biology
Hiba Abu Hariri, Ilana Braunstein, Talal Salti, Fabian Glaser, Tal Gefen, Naama Geva-Zatorsky, Tamar Ziv, Moran Benhar
Summary: This study characterized the dynamic cysteine proteome of human macrophages and identified the role of thiol redox control in regulating inflammatory responses. The findings provide insights into the adaptability of macrophages to changing inflammatory and redox environments.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemical Research Methods
Puneet Seth, Richard T. Premont, Jonathan S. Stamler
Summary: Post-translational modification by S-nitrosylation plays a key role in regulating cellular functions and impacting proteins across phylogeny. The protocol described focuses on isolating S-nitrosylated proteins from C. elegans, with potential for adaptation to mammalian tissues. Protecting SNO proteins from degradation remains a major challenge in this protocol.
Article
Biochemistry & Molecular Biology
Claudia Cirotti, Salvatore Rizza, Paola Giglio, Noemi Poerio, Maria Francesca Allega, Giuseppina Claps, Chiara Pecorari, Ji-Hoon Lee, Barbara Benassi, Daniela Barila, Caroline Robert, Jonathan S. Stamler, Francesco Cecconi, Maurizio Fraziano, Tanya T. Paull, Giuseppe Filomeni
Summary: This study demonstrates that GSNOR is induced at the translational level in response to hydrogen peroxide and mitochondrial ROS, and that the modulation of ATM/GSNOR axis affects cell survival and mitophagy. This interplay also regulates T-cell activation, providing new insights into the pleiotropic effects of ATM in the context of immune function.
Article
Biochemistry & Molecular Biology
Alaa Knany, Rotem Engelman, Hiba Abu Hariri, Shyam Biswal, Haguy Wolfenson, Moran Benhar
FREE RADICAL BIOLOGY AND MEDICINE
(2020)