Article
Chemistry, Medicinal
Ozlem Akgul, Andrea Angeli, Silvia Selleri, Clemente Capasso, Claudiu T. Supuran, Fabrizio Carta
Summary: New taurultambenzenesulfonamides were synthesized and evaluated for their inhibitory activity against multiple Carbonic Anhydrases. Some compounds showed potent inhibition against VchCA alpha and BpsCA-gamma, indicating potential for the development of new generation CA-based antibiotics.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Hamada H. Amer, Essam Hassan Eldrehmy, Salama Mostafa Abdel-Hafez, Youssef Saeed Alghamdi, Magdy Yassin Hassan, Saad H. Alotaibi
Summary: A series of nucleosides, moieties, and Schiff bases were synthesized from sulfadimidine in this study. After purification using chromatographic techniques, the compounds were subjected to molecular docking studies and tested for antibacterial activity against Staphylococcus aureus, E. coli, and Klebsiella pneumoniae. The most effective compounds were identified for each bacterium, with promising antibacterial effects observed in vitro. Further research is needed to explore the in vivo antibacterial effects and therapeutic doses of these compounds.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Mehmet Onyilmaz, Murat Koca, Alessandro Bonardi, Mustafa Degirmenci, Claudiu T. Supuran
Summary: Isocoumarins were investigated as inhibitors of carbonic anhydrase, and their interaction with four human CA isoforms was studied. It was found that isocoumarins inhibited hCA IX and XII in the low micromolar range, while hCA I and II were not affected. The formation of 2-carboxy-phenylacetic aldehydes was hypothesized as the mechanism of inhibition.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Environmental Sciences
Yan-Hui Bi, An-Ying Du, Jia-Li Li, Zhi-Gang Zhou
Summary: Saccharina japonica, a dominant seaweed in cold-temperate rocky shores, shows high photosynthetic efficiency and may possess a carbon-concentrating mechanism (CCM) involving the crucial role of carbonic anhydrase (CA). This study identified and characterized a specific CA in S. japonica, suggesting its potential involvement in the CCM. The enzyme was found to be localized in the mitochondria and up-regulated by low CO2 concentration, indicating its importance in the seaweed's CCM.
Article
Biochemistry & Molecular Biology
Simone Giovannuzzi, Viviana De Luca, Alessio Nocentini, Clemente Capasso, Claudiu T. Supuran
Summary: Coumarins have been found to be inhibitors of alpha-carbonic anhydrases, and this study confirms that they effectively inhibit the eta-CA enzyme. The investigation also reveals that eta-CAs possess esterase activity, which is not present in other known classes of CAs.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Simone Giovannuzzi, Viviana De Luca, Clemente Capasso, Claudiu T. Supuran
Summary: The gamma-carbonic anhydrase (CA, EC 4.2.1.1) from Mammaliicoccus (Staphylococcus) sciuri (MscCA gamma) was studied for its inhibition by various (in)organic anions. Peroxydisulfate and trithiocarbonate were the most effective inhibitors, while other anions showed weaker inhibitory effects. Finding more effective MscCA gamma inhibitors could help combat antibiotic resistance.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Arun Kumar Mishra, Vachan Singh, Arvind Kumar, Harpreet Singh
Summary: This study synthesized a series of 2-aminobenzothiazole derivatives as anticonvulsant agents and conducted research on their biological activities and molecular docking. The results showed that V-5 exhibited the strongest anticonvulsant effect and could be a promising lead molecule for the development of new anticonvulsant drugs.
LETTERS IN DRUG DESIGN & DISCOVERY
(2022)
Article
Chemistry, Medicinal
Davide Ialongo, Antonella Messore, Valentina Noemi Madia, Valeria Tudino, Alessio Nocentini, Paola Gratteri, Simone Giovannuzzi, Claudiu T. T. Supuran, Alice Nicolai, Susanna Scarpa, Samanta Taurone, Michele Camarda, Marco Artico, Veronica Papa, Francesco Saccoliti, Luigi Scipione, Roberto Di Santo, Roberta Costi
Summary: Solid tumors create a hostile environment for surrounding cells by decreasing pH through producing metabolic acids. Carbonic anhydrases (CAs) play a role in acid/base regulation and are clinically relevant in cancer therapy. This study describes new diketo acid derivatives as nanomolar inhibitors of the CA IX and XII isoforms, with selectivity over CAs I and II. These compounds showed antiproliferative activity in tumor cell lines overexpressing CA IX and XII, with no effect on normal cells.
Article
Biochemistry & Molecular Biology
Amena Ali, Abuzer Ali, Musarrat Husain Warsi, Mohammad Akhlaquer Rahman, Mohamed Jawed Ahsan, Faizul Azam
Summary: This study utilized co-crystallized inhibitors for pharmacophore-based virtual screening and molecular simulation, identifying several compounds that show potential as inhibitors for high-altitude disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Morteza Abdoli, Alessandro Bonardi, Claudiu T. Supuran, Raivis Zalubovskis
Summary: A one-pot two-step protocol was developed for the synthesis of novel 4-cyanamidobenzenesulfonamides, which showed effective inhibition towards both human and bacterial carbonic anhydrase enzymes.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Morteza Abdoli, Claudiu T. Supuran, Raivis Zalubovskis
Summary: A small library of substituted cyclic guanidine incorporated benzothiazole-6-sulphonamides was synthesized and evaluated for their inhibitory activity against brain-associated human carbonic anhydrase isoforms. The results demonstrated that these compounds exhibited potent and selective inhibition towards hCA II and VII, while hCA I showed lower sensitivity to inhibition. These derivatives may serve as promising candidates for the design of more potent and selective hCA II and VII inhibitors.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biophysics
Iryna Antal, Martina Koneracka, Martina Kubovcikova, Vlasta Zavisova, Alena Jurikova, Iryna Khmara, Maria Omastova, Matej Micusik, Monika Barathova, Lenka Jelenska, Ivana Kajanova, Miriam Zatovicova, Silvia Pastorekova
Summary: Antibody-modified magnetic nanoparticles were successfully prepared and demonstrated the ability to target cancer cells specifically by conjugating specific monoclonal antibodies on their surface. The internalization of these nanoparticles into tumor cells expressing hypoxic marker was confirmed, showing their potential for targeted cancer therapy.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Medicine, Research & Experimental
Na Xu, Wujie Lu, Lijie Meng, Xu Feng, Jingjing Xuan, Fangfang Liu, Zhijun Feng
Summary: The synthesized SiO2 nanoparticles showed potential activity in inhibiting carbonic anhydrase, antioxidant stress, and antibacterial effects, providing valuable information for the development of platforms for pneumonia treatment and management.
Article
Chemistry, Multidisciplinary
Fuying Zhu, Haochen Qiu, Fei Wang, Xing Zhang, Guo-Ping Lu, Yamei Lin, He Huang
Summary: Stable and cost-effective carbonic anhydrase (CA)-like nanozymes, such as Fe10@CN-Mg, are efficient and sustainable for CO2 fixation. Fe10@CN-Mg, a novel iron-based nanomaterial, acts as a CA mimic with FeNx sites and Mg(OH)2 synergistically catalyzing CO2 conversion. It shows comparable kinetic constants, higher CaCO3 formation rate, and remains stable and active under extreme conditions and long storage times. Fe10@CN-Mg has been successfully applied for CO2 conversion into cyclic carbonates with high economic efficiency.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Maan T. Khayat, Hany E. A. Ahmed, Abdelsattar M. Omar, Yosra A. Muhammad, Khadijah A. Mohammad, Azizah M. Malebari, Ahdab N. Khayyat, Ahmed H. Halawa, Hamada S. Abulkhair, Ahmed A. Al-Karmalawy, Mohammed Almaghrabi, Majed Alharbi, Anfal S. Aljahdali, Ahmed M. El-Agrody
Summary: This study developed a more rigid ring system with a sulfonamide hydrophilic head and a lipophilic tail to synthesize novel molecules with better selectivity. The activity and selectivity of these molecules were extensively discussed through in vitro cytotoxicity evaluation, structure-activity relationship, and carbonic anhydrase enzyme assay. The results showed that all the new candidates exhibited good cytotoxic activities against breast and colorectal carcinomas, with compounds 22, 24, and 27 selectively inhibiting hCA IX. Furthermore, compound 27 demonstrated the potential to decrease the wound closure percentage in MCF-7 cells. Molecular docking and molecular orbital analysis revealed the potential binding interactions of compounds 24 and 27 with crucial amino acids of hCA IX.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Genetics & Heredity
Cas Simons, Laurie B. Griffin, Guy Helman, Gretchen Golas, Amy Pizzino, Miriam Bloom, Jennifer L. P. Murphy, Joanna Crawford, Sarah H. Evans, Scott Topper, Matthew T. Whitehead, John M. Schreiber, Kimberly A. Chapman, Cyndi Tifft, Katrina B. Lu, Howard Gamper, Megumi Shigematsu, Ryan J. Taft, Anthony Antonellis, Ya-Ming Hou, Adeline Vanderver
AMERICAN JOURNAL OF HUMAN GENETICS
(2015)
Editorial Material
Biochemistry & Molecular Biology
Ya-Ming Hou, Howard Gamper, Wei Yang
Article
Multidisciplinary Sciences
Howard B. Gamper, Isao Masuda, Milana Frenkel-Morgenstern, Ya-Ming Hou
NATURE COMMUNICATIONS
(2015)
Article
Biochemistry & Molecular Biology
Marni J. Falk, Xiaowu Gai, Megumi Shigematsu, Elisa Vilardo, Ryuichi Takase, Elizabeth McCormick, Thomas Christian, Emily Place, Eric A. Pierce, Mark Consugar, Howard B. Gamper, Walter Rossmanith, Ya-Ming Hou
Article
Biochemistry & Molecular Biology
Howard Gamper, Ya-Ming Hou
Article
Chemistry, Physical
Agata P. Perlinska, Marcin Kalek, Thomas Christian, Ya-Ming Hou, Joanna Sulkowska
Review
Cell Biology
Ya-Ming Hou, Isao Masuda, Leonard J. Foster
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2020)
Article
Genetics & Heredity
Howard Gamper, Ya-Ming Hou
Article
Multidisciplinary Sciences
Howard Gamper, Haixing Li, Isao Masuda, D. Miklos Robkis, Thomas Christian, Adam B. Conn, Gregor Blaha, E. James Petersson, Ruben L. Gonzalez, Ya-Ming Hou
Summary: The study investigates the translation of quadruplet codons by a +1-frameshifting tRNA SufB2, revealing that SufB2 initially uses triplet anticodon-codon pairing in the 0-frame to decode the quadruplet codon before shifting to the +1-frame during tRNA-mRNA translocation. The frameshifting mechanism of SufB2 involves perturbation of a crucial ribosome conformational change, highlighting the potential for engineering specific ribosome conformational changes to enhance genome recoding efficiency.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Howard Gamper, Yujia Mao, Isao Masuda, Henri McGuigan, Gregor Blaha, Yuhong Wang, Shoujun Xu, Ya-Ming Hou
Summary: By utilizing the high-efficiency +1-frameshifting SufB2 tRNA, it was discovered that there are two explorations of +1 frameshifting in one elongation cycle of protein synthesis, with the majority occurring during translocation from the A site to the P site. This research demonstrates that the ribosome itself plays a critical role in determining changes in the reading frame, providing insights into the mechanistic parallel of +1 frameshifting with -1 frameshifting.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Gabriel Demo, Howard B. Gamper, Anna B. Loveland, Isao Masuda, Christine E. Carbone, Egor Svidritskiy, Ya-Ming Hou, Andrei A. Korostelev
Summary: The study found that the ribosome and EF-G cooperate to induce +1 frameshifting during tRNA-mRNA translocation.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Isao Masuda, Jae-Yeon Hwang, Thomas Christian, Sunita Maharjan, Fuad Mohammad, Howard Gamper, Allen R. Buskirk, Ya-Ming Hou
Summary: N-1-methylation of G37 is crucial for maintaining the translational reading-frame of a subset of tRNAs, with its deficiency leading to ribosome stalling during decoding of affected codons and affecting tRNA aminoacylation and peptide-bond formation. This study highlights a previously unrecognized role of m(1) G37 throughout the elongation cycle of protein synthesis, shedding light on its essentiality for bacterial growth and survival.
Article
Multidisciplinary Sciences
Zhicheng Cui, Xiaojun Li, Joonyoung Shin, Howard Gamper, Ya-Ming Hou, James C. Sacchettini, Junjie Zhang
Summary: The authors present structures of the MtbEttA protein bound to or free of the ribosome from Mycobacterium tuberculosis under different nucleotide states, which provide insights into the mechanism of translational regulation by EttA-like proteins.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Sepideh Tavakoli, Mohammad Nabizadeh, Amr Makhamreh, Howard Gamper, Caroline A. McCormick, Neda K. Rezapour, Ya-Ming Hou, Meni Wanunu, Sara H. Rouhanifard
Summary: Here, researchers developed a semi-quantitative method using direct long-read nanopore sequencing to accurately identify pseudouridylated sites on mammalian mRNAs. By adjusting specific parameters, they successfully identified known and previously unreported pseudouridylated sites. The method also allowed for the detection of multiple modifications on the same RNA strand. Overall, this research provides valuable insights into RNA modifications and receives a score of 9 out of 10.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Isao Masuda, Ya-Ming Hou
Summary: We present a genome-wide screening approach for identifying the most critical core reaction supported by an essential gene for cell viability. The protocol includes steps for constructing maintenance plasmids, knockout cells, and validating phenotypes. We also outline the process of isolating suppressors, conducting whole-genome sequencing analysis, and reconstructing CRISPR mutants. The focus of our study is the E. coli trmD gene, which encodes an essential methyl transferase involved in synthesizing m1G37 on the 30-side of the tRNA anticodon. For more detailed information and implementation of this protocol, please refer to Masuda et al. (2022).
