期刊
BIOCHEMISTRY
卷 47, 期 3, 页码 1070-1075出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi7021359
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资金
- NEI NIH HHS [EY05216, R01 EY005216, EY11500, R01 EY005216-25, R01 EY011500-12, R37 EY005216, R01 EY011500] Funding Source: Medline
- NIGMS NIH HHS [GM07628, R01 GM077561, T32 GM007628, R01 GM077561-01A1] Funding Source: Medline
The robust cooperative formation of rod arrestin tetramers has been well-established, whereas the ability of other members of the arrestin family to self-associate remains controversial. Here, we used purified arrestins and multi-angle light scattering to quantitatively compare the propensity of the four mammalian arrestin subtypes to self-associate. Both non-visual and cone arrestins only form oligomers at very high non-physiological concentrations. However, inositol hexakisphosphate (IP6), a fairly abundant form of inositol in the cytoplasm, greatly facilitates self-association of arrestin2. Arrestin2 self-association equilibrium constants in the presence of 100 mu M IP6 suggest that an appreciable proportion could exist in an oligomeric state but only in intracellular compartments where its concentration is 5-10-fold higher than average. In contrast to arrestin2, IP6, inhibits self-association of rod arrestin, indicating that the structure of these two tetramers in solution is likely different.
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