Article
Biochemistry & Molecular Biology
Dorothee Houry, Arne Raasakka, Eugenio Ferrario, Marc Niere, Ersilia Bifulco, Petri Kursula, Mathias Ziegler
Summary: In this study, the mechanism of increased substrate affinity of phosphorylated NAMPT is revealed, which includes the coordination of Mg2+ by phospho-His247 to stabilize PRPP in a position favorable for catalysis. Additionally, a stretch of 10 amino acids found only in NAMPTs from deuterostomes is shown to enhance conformational plasticity and stabilize the chemically unstable phosphorylation of His247. Overall, this study provides a structural basis for the important function of NAMPT in recycling Nicotinamide into NAD biosynthesis with high affinity.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)
Article
Chemistry, Physical
A. Debski, M. Peska, J. Dworecka-Wojcik, S. Terlicka, W. Gasior, W. Gierlotka, M. Polanski
Summary: Despite years of research on the affinities of palladium and magnesium with hydrogen, information on magnesium-palladium alloys remains incomplete. While magnesium palladium alloys are unlikely to be used as solid-state hydrogen storage materials, their interaction with hydrogen may be useful for broadening knowledge or finding other applications.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Biochemical Research Methods
Xiaofei Wu, Mengying Zhang, Zhiling Kuang, Jian Yue, Lu Xue, Min Zhu, Zhongliang Zhu, Muhammad Hidayatullah Khan, Liwen Niu
Summary: Crystal structures of Saccharomyces cerevisiae Anthranilate phosphoribosyltransferase (ScAnPRT) in apo form, bound with its substrate PRPP and substrate analogue 4-fluoroanthranilate (4FA), were determined. The structures show the conserved fold and substrate binding mode of type III phosphoribosyltransferase enzymes, with insights into the importance of specific residues for substrate binding. These structures enhance understanding of ScAnPRT substrate binding and lay the groundwork for future research.
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Christo N. Nanev, Emmanuel Saridakis, Naomi E. Chayen
Summary: X-ray crystallography is currently the dominant method for determining the structure of proteins and biomolecules. However, it faces challenges in obtaining high-resolution data from small protein crystals. Serial crystallography using XFELs is a promising alternative method that can produce excellent structural data from weakly diffracting objects. In this study, the authors calculate the mean crystal size and growth time needed for batch crystallization, which is the main method for preparing microcrystalline slurries. They also consider the impact of impurities on the growth of microcrystals. Experimental results with lysozyme support the theoretical predictions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Inorganic & Nuclear
Catalin Maxim, Cristian D. Ene, Ioana Nicolau, Lavinia L. Ruta, Ileana C. Farcasanu
Summary: Three pairs of copper(II) complexes with tridentate Schiff bases were synthesized and used for biological assessments. The structures, spectra, and biological activities of the complexes were analyzed, and the accumulation of 3-S was found to potentially disrupt the redox state of cells.
DALTON TRANSACTIONS
(2022)
Article
Chemistry, Applied
Sofia F. Reis, Susana Messias, Rita Bastos, Vitor J. Martins, Viviana G. Correia, Benedita A. Pinheiro, Lisete M. Silva, Angelina S. Palma, Manuel A. Coimbra, Elisabete Coelho
Summary: Brewing practice involves reusing the same yeast for subsequent fermentations. The yeast used for Lager beer (Saccharomyces pastorianus) is widely reused without changes in fermentation performance. However, the yeast used for Ale beer (Saccharomyces cerevisiae) is either partially or not reused due to poor performance. The study found that cells modulate their wall polysaccharides to increase cell wall strength.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Physical
Yogesh Kumar, S. Tripathi, Mangla Nand, R. Jangir, V. Srihari, A. Das, R. Singh, U. Deshpande, S. N. Jha, A. Arya
Summary: This study investigated the effect of Nd doping on the structural, optical, and electronic properties of lanthanum monazite structure. Pure and Nd-doped LaPO4 powders were prepared and analyzed for their composition, oxidation states, crystal structure, and bandgaps. The results confirmed the successful substitution of Nd in the LaPO4 matrix and provided insights into the local structure using extended X-ray absorption fine structure analysis.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Biotechnology & Applied Microbiology
Javier Garcia-Pardo, Aleksandra E. Badaczewska-Dawid, Carlos Pintado-Grima, Valentin Iglesias, Aleksander Kuriata, Sebastian Kmiecik, Salvador Ventura
Summary: This study presents the A3D yeast database (A3DyDB), which provides an extensive catalog of aggregation propensity predictions for the S. cerevisiae proteome, allowing for the evaluation of the influence of mutations on protein stability and solubility.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Stephane Rety, Yingzi Zhang, Wentong Fu, Shan Wang, Wei-Fei Chen, Xu-Guang Xi
Summary: Pif1 proteins play important roles in replication and genome stability in eukaryotes, but their functions in bacteria are less understood. Some Pif1 proteins from thermophilic bacteria contain a C-terminal WYL domain, which may enhance their nucleic acid binding ability in high-temperature conditions.
Article
Chemistry, Physical
Isha Charak, M. Manhas, A. K. Bedyal, Sukhbir Singh, Anurag Srivastava, H. C. Swart, Vinay Kumar
Summary: Highly pure red light emitting Ca3B2O6:Eu(3+) phosphor was synthesized via a solution combustion method for potential application in white light emitting diodes. The synthesized phosphor exhibited characteristic emission peaks under near UV excitation, with short decay time, strong red emission, and high color purity. The structural, spectral, and luminescent properties of the phosphor were studied using XRD, FTIR, XPS, and PL spectroscopy.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Food Science & Technology
Junyan Liu, Tengyi Huang, Wei Hong, Fang Peng, Zerong Lu, Gongyong Peng, Xin Fu, Gongliang Liu, Zhi Wang, Qingmei Peng, Xiangjun Gong, Lizhen Zhou, Lin Li, Bing Li, Zhenbo Xu, Haifeng Lan
Summary: The study found that HPUL treatment induced stress response and altered cell metabolism of S. cerevisiae, potentially leading to failure of deactivation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
N. P. Samantray, R. N. P. Choudhary
Summary: This study reported the structural and electrical properties of Ca/Zr modified polycrystalline BiFeO3 samples prepared by solid-state reaction method, revealing the formation of single phase perovskite in rhombohedral phase and the ferroelectricity in the materials. Analysis of X-ray diffraction data and scanning electron microscope images provided insights into the micro-structure, while dielectric, impedance and conductivity studies offered information on the dielectric properties and conductivity of the materials. Additionally, the contribution of grains and grain boundaries towards the total resistance and capacitance was determined from Nyquist plots. The J-E characteristics demonstrated Ohmic behavior with a slope close to 1.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Article
Materials Science, Ceramics
Hui Wei, Jing Li, Feng Hu, Yang Han, Bensong Chen, Honglin Li, Yang Zhang, Xiaodong Wang, Xiaoyong Xu
Summary: This study analyzed the characteristics and crystal structure of ZrW2-xMoxO8 (0≤x≤1). The materials exhibited a systematic change in the coefficient of thermal expansion, from negative values to zero thermal expansion, with increasing x. The study also investigated the mechanism of the ordered-disordered phase transition and the impact of Mo substitution on the electronic structure using in-situ X-ray diffraction and in-situ Raman spectroscopy.
CERAMICS INTERNATIONAL
(2023)
Article
Materials Science, Multidisciplinary
M. K. Shobana, Hyeji Park, Heeman Choe
Summary: In this study, Sr-doped Co nanoferrites were synthesized using a sol-gel combustion method, and the correlation between Sr substitution in Co ferrite and the structural and optical changes was discussed. The average crystallite sizes, lattice parameters, spinel structure, and bandgap energy values of the nanoferrites were investigated. The results indicated that Sr substitution affected the crystal structure and optical properties of Co ferrite nanoparticles.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Yanping Hu, Yan Wang, Jaideep Singh, Ruirui Sun, Lilei Xu, Xiaolin Niu, Keyun Huang, Guangcan Bai, Guoquan Liu, Xiaobing Zuo, Chunlai Chen, Peter Z. Qin, Xianyang Fang
Summary: Pulsed electron-electron double resonance (PELDOR) spectroscopy, X-ray scattering interferometry (XSI), and single-molecule Forster resonance energy transfer (smFRET) are molecular rulers that provide inter- or intramolecular pair-wise distance distributions in the nanometer range, thus being ideally suitable for structural and dynamic studies of biomolecules including RNAs. However, the current labeling strategies may cause structural perturbation and decrease the accuracy of measurements.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sun-Mi Shin, Jin Myung Choi, Eric di Luccio, Yong-Jik Lee, Sang-Jae Lee, Sang Jun Lee, Sung Haeng Lee, Dong-Woo Lee
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2015)
Article
Biochemistry & Molecular Biology
Jin Myung Choi, Yong-Jik Lee, Thinh-Phat Cao, Sun-Mi Shin, Min-Kyu Park, Han-Seung Lee, Eric di Luccio, Seong-Bo Kim, Sang -Jae Lee, Sang Jun Lee, Sung Haeng Lee, Dong-Woo Lee
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2016)
Article
Biochemical Research Methods
Damiaan E. H. F. Mevius, Yunpeng Shen, Masayo Morishita, Eric Di Luccio
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2016)
Article
Chemistry, Medicinal
Masayo Morishita, Damiaan E. H. F. Mevius, Yunpeng Shen, Shuyu Zhao, Eric di Luccio
MEDICINAL CHEMISTRY RESEARCH
(2017)
Article
Multidisciplinary Sciences
Eric Di Luccio, Yuko Ishida, Walter S. Leal, David K. Wilson
Article
Biophysics
Masayo Morishita, Damiaan Mevius, Eric di Luccio
BMC STRUCTURAL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Yunpeng Shen, Masayo Morishita, Doohyun Lee, Shinae Kim, Taeho Lee, Damiaan E. H. F. Mevius, Yeonjeong Roh, Eric di Luccio
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Yunpeng Shen, Damiaan E. H. F. Mevius, Rocco Caliandro, Benedetta Carrozzini, Yeonjeong Roh, Jihyeon Kim, Sunghwan Kim, Sung Chul Ha, Masayo Morishita, Eric di Luccio
Article
Biochemical Research Methods
Yunpeng Shen, Masayo Morishita, Eric di Luccio
PROTEIN EXPRESSION AND PURIFICATION
(2020)
Review
Biochemistry & Molecular Biology
Hiromichi Sato, Kazuki Sasaki, Tomoaki Hara, Yoshiko Tsuji, Yasuko Arao, Chihiro Otsuka, Yumiko Hamano, Mirei Ogita, Shogo Kobayashi, Eric di Luccio, Takaaki Hirotsu, Yuichiro Doki, Hidetoshi Eguchi, Taroh Satoh, Shizuka Uchida, Hideshi Ishii
Summary: This article describes the causes and complexity of pancreatic ductal adenocarcinoma (PDAC), emphasizing the importance of early diagnosis and the multifaceted characteristics of PDAC in terms of cancer cell metabolism, mesenchymal cells, and metagenomics. The article also mentions various pathway alterations associated with PDAC treatment refractoriness and prospects for the application of new technologies.
Review
Biochemistry & Molecular Biology
Eric di Luccio, Masayo Morishita, Takaaki Hirotsu
Summary: Improving the rate and effectiveness of routine cancer screenings is a challenge in healthcare, but multi-cancer early detection (MCED) is a potentially effective solution. Using C. elegans as a biosensor for universal cancer screening, specifically with N-NOSE, has shown promising results.
Article
Biochemistry & Molecular Biology
Toshimi Sugimoto, Yozo Okuda, Ayaka Shima, Natsuko Sugiura, Nobuaki Kondo, Genki Ishihara, Takaaki Hirotsu, Eric di Luccio
Summary: Cancer is the leading cause of death in dogs and cats, and early diagnosis is crucial. N-NOSE is a non-invasive cancer screening test that detects 15 types of human cancer. This study evaluated the effectiveness of N-NOSE in detecting cancer using urine samples from dogs and cats, and found promising results.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Junichi Yamaguchi, Eric di Luccio, Takaaki Hirotsu
Review
Biochemistry & Molecular Biology
Umbhorn Ungkulpasvich, Hideyuki Hatakeyama, Takaaki Hirotsu, Eric di Luccio
Summary: The pancreas is an important organ with exocrine and endocrine functions. Pancreatitis, caused by alcohol consumption and gallstones, increases the risk of pancreatic cancer. Early detection is crucial for improving pancreatic cancer outcomes. Diagnostic methods, including imaging modalities and tissue biopsy, aid in the detection and analysis of pancreatic cancer. Liquid biopsy shows promise in early tumor detection by assessing biomarkers in bodily fluids. Understanding the function of the pancreas, associated diseases, risk factors, and available diagnostic methods is essential for effective management and early pancreatic cancer detection.
Article
Biochemistry & Molecular Biology
Eric di Luccio, Satoru Kaifuchi, Nobuaki Kondo, Ryota Chijimatsu, Andrea Vecchione, Takaaki Hirotsu, Hideshi Ishii
Summary: Nematodes, specifically Caenorhabditis elegans, have been crucial in cancer research for their role in mechanism analysis, drug discovery, and cancer biodiagnostics. They have contributed to the study of cancer genome defects, diagnosis of refractory tumors, and evaluation of cell invasion. Nematode-applied technology is expected to advance research on the human tumor microenvironment.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)