Article
Chemistry, Physical
Margareta R. A. Blomberg
Summary: Improvements in density functional theory methodology have made it a powerful tool in studying enzymatic reaction mechanisms, but concerns remain about the reliability of results for metalloenzymes. By using different DFT functionals and combining with experimental data, stable qualitative pictures of reaction mechanisms were obtained.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Rose Syuhada Basri, Raja Noor Zaliha Raja Abd Rahman, Nor Hafizah Ahmad Kamarudin, Mohd Shukuri Mohamad Ali
Summary: Biocatalysts have gained attention for their industrial-relevant properties, which can contribute to a cleaner environment. Carboxylic acid reductases (CARs) are large proteins that can convert carboxylic acids to aldehydes with the presence of ATP and NADPH. CARs have potential applications in various industrial processes, and recent studies have provided valuable insights into their structure, mechanisms, and immobilization approaches. This comprehensive review summarizes previous findings on CARs' phylogeny, structure, mechanism, post-translational modification, cofactor regeneration, industrial applications, and immobilization techniques.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Joana R. Exposito, Eva Barreno, Myriam Catala
Summary: NO plays a key role in abiotic stress tolerance and symbioses establishment. Lichens biosynthesize NO through both oxidative and reductive enzymatic pathways, with NR activity being the main source of NO biosynthesis. However, NR activity in lichens shows interesting features that deserve further study.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Chemistry, Physical
Maria C. Martins, Susana F. Fernandes, Bruno A. Salgueiro, Jessica C. Soares, Celia Romao, Claudio M. Soares, Diana Lousa, Filipe Folgosa, Miguel Teixeira
Summary: Flavodiiron proteins are a family of enzymes with nitric oxide and/or oxygen reductase activities. Mutants with altered conserved residues showed reduced oxygen reductase activity and in some cases completely abolished NO reductase activity, possibly due to changes in predicted structures affecting hydration and hydrogen bonds.
Article
Energy & Fuels
Richuan Rao, Hanwen Liang, Chunming Hu, Huaze Dong, Xiongzi Dong, Yongqiang Tang, Song Fang, Qiang Ling
Summary: Ag-CeO2 catalysts were synthesized using different methods to study their catalytic performance in CO combustion. The surface chemical state of Ag and CeO2 nanoparticles significantly influenced the interaction at the Ag-CeO2 interface. Urea-assisted pyrolysis resulted in an interface with more metallic Ag species, improving the reductivity of CeO2 and enhancing the catalyst performance. Activated carbon-assisted pyrolysis led to an interface with more positively charged Ag+ ions, requiring higher CO conversion temperature. The addition of sodium salts promoted the transformation of the Ag-CeO2 interface, resulting in more Ce3+ cations and enhanced catalyst performance.
Article
Chemistry, Physical
Jingyuan Zhuang, Fan Zhang, Xiaowen Tang, Chengzhi Liu, Min Huang, Hujun Xie, Ruibo Wu
Summary: This study explores the catalytic mechanism of bCinS enzyme through detailed multiscale simulations, revealing the crucial roles of protein conformational change and ligand flexibility in regulating catalytic selectivity. The importance of fleeting chiral intermediates and key structural factors for the conformational transformation of the active pocket are also discussed.
CATALYSIS SCIENCE & TECHNOLOGY
(2022)
Article
Plant Sciences
Bharat Bhusan Majhi, Sarah-Eve Gelinas, Natacha Merindol, Simon Ricard, Isabel Desgagne-Penix
Summary: This study elucidates the involvement of both norbelladine synthase (NBS) and noroxomaritidine/norcraugsodine reductase (NR) in the biosynthesis of norbelladine in Amaryllidaceae plants. They function together by physically interacting, co-localizing, and co-expressing in the cytoplasm and nucleus. The study also confirms their ability to catalyze the key steps associated with the biosynthesis of the Alzheimer's drug galanthamine.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Energy & Fuels
Zhiping Lei, Meichen Liu, Jingchong Yan, Tiejun Chun, Jia Fang, Zhanku Li, Hengfu Shui, Shibiao Ren, Zhicai Wang, Xianzhong Cao, Ying Kong, Shigang Kang
Summary: Steel scale (SS) is proven to be a promising catalyst for catalytic combustion of coke nuts and in-situ reduction of NO emissions, showing potential for significant reduction in NO emission levels.
Article
Biochemistry & Molecular Biology
Natalia Napieraj, Malgorzata Janicka, Beata Augustyniak, Malgorzata Reda
Summary: This study reveals the important regulatory role of exogenous putrescine (Put) in nitric oxide (NO) production in cucumber roots under salt stress conditions. Put reduces NO levels in short-term salt stress, but increases NO levels in long-term salt stress. Additionally, Put modulates NO biosynthesis pathways and affects nitrate reductase (NR) activity to regulate salt stress response.
Article
Agricultural Engineering
Zeynab Azimychetabi, Manije Sabokdast Nodehi, Taraneh Karami Moghadam, Babak Motesharezadeh
Summary: Cadmium stress induced oxidative stress in peppermint plants, leading to changes in menthol content and the expression of key genes involved in essential oil biosynthesis. The study found that menthol content decreased and menthofuran and pulegone concentrations increased under increasing cadmium levels. Additionally, there were changes in biochemical traits such as malondialdehyde, hydrogen peroxide, proline content, and enzyme activity in response to cadmium stress.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Chemistry, Organic
Ryosuke Tanaya, Takeshi Kodama, Yuan-E Lee, Yoko Yasuno, Tetsuro Shinada, Hironobu Takahashi, Takuya Ito, Hiroyuki Morita, Suresh Awale, Futoshi Taura
Summary: This study explores the synthesis of various CBGA analogues, including a liverwort-derived bibenzyl acid precursor, using CsPT4. The synthesized natural and new-to-nature cannabinoids exhibit potent cytotoxicity in human pancreatic cancer cells. CsPT4 has the potential to expand the diversity of cannabinoid biosynthesis with novel and improved biological activities.
Article
Chemistry, Inorganic & Nuclear
Wenting Zhou, Wentong Jing, Hui Shen, Ruixuan Qin, Shiguang Mo, Mengfei Qiao, Gang Fu, Nanfeng Zheng
Summary: The promoting effect of the interface between metal and TiOx structure in heterogeneous catalysis is widely recognized. However, due to the complex structural parameters in heterogeneous metal catalysts, understanding the interface at the molecular level is a challenge. In this study, a CuTiOx/Al2O3 catalyst with a well-defined Cu(II)-TiOx interface was synthesized using a Cu-containing polyoxotitanate as the precursor. The catalyst showed higher deNO(x) activity in the NH3-SCR reaction compared to Cu/Al2O3 with isolated Cu(II).
JOURNAL OF CLUSTER SCIENCE
(2023)
Article
Chemistry, Inorganic & Nuclear
Miljan Z. Corovic, Fabian Wiedemaier, Ferdinand Belaj, Nadia C. Mosch-Zanetti
Summary: By replacing molybdenum with tungsten, the derived enzyme from the Rhodobacteraceae family catalyzes DMSO reduction faster. Tungsten complexes can undergo oxygen atom transfer reactions with excess PMe3, forming tungsten(IV) oxido species. This study sheds light on the mechanism of tungsten in reduction reactions.
INORGANIC CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Jialiang Wang, Zixin Deng, Jingdan Liang, Zhijun Wang
Summary: Iterative type I polyketide synthases (iPKSs) are megaenzymes that synthesize complex natural products with diverse bioactivities. The programmed patterns of domain combinations during the synthetic cycle of iPKSs strongly influence the complexity of the resulting natural products. This article reviews the structural enzymology of iPKSs, focusing on individual domain structures and their representative programming activities.
NATURAL PRODUCT REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Ziguo Cai, Guodong Zhang, Zhicheng Tang, Jiyi Zhang
Summary: By adjusting the thickness of the CeO2 shell layer, MnFe@CeOx core-shell nanocages were synthesized, which exhibited excellent NO removal efficiency and H2O resistance at higher temperatures. The catalyst had abundant oxygen vacancy defect sites, surface acid sites, and more active species with strong interactions. The construction of the CeO2 shell remarkably enhanced catalytic performance and H2O resistance.
ACS APPLIED NANO MATERIALS
(2022)