Article
Biochemistry & Molecular Biology
Jianong Zhang, Liang Zhang, Ji Nie, Yan Lin, Yao Li, Wei Xu, Jian-Yuan Zhao, Shi-Min Zhao, Chenji Wang
Summary: Calcineurin interacts with the mitochondrial gatekeeper enzyme PDC to enhance cellular metabolism and suppress cancer cell proliferation. Inhibition of the calcineurin-PDC pathway by CNIs may promote tumorigenesis, suggesting a potential mechanism for the increased cancer incidence in transplant recipients.
Article
Pharmacology & Pharmacy
Marie Fichter, Gesa Richter, Alexander Bepperling, Paul Wassmann
Summary: This study systematically assessed the factors and characteristics governing the formation of immune complexes (ICs) in vitro. The interplay between the absolute concentration of the involved components, their molar ratios, structural features of the biologic, and its endogenous target was demonstrated. The study provides valuable insights into IC formation mechanisms and can be applied to various biotherapeutics.
Editorial Material
Hematology
Monika Haemmerle
Summary: This study explores the role of Mnk1 in megakaryocytes and platelets, showing that it regulates protein synthesis and affects megakaryocyte ploidy and platelet production. These findings have clinical implications and contribute to our understanding of platelet function and thrombosis.
Article
Chemistry, Applied
Haobo Jin, Peishan Li, Yongguo Jin, Long Sheng
Summary: The study investigated the mechanism by which sodium tripolyphosphate affected the aggregation behavior of ovalbumin-lysozyme complexes. The results indicated that phosphorylation increased turbidity, weakened structure unfolding and stretching, and resulted in a broader network structure of the complex.
Article
Cell Biology
Haibo Dong, Yue Feng, Yang Yang, Yun Hu, Yimin Jia, Shu Yang, Nannan Zhao, Ruqian Zhao
Summary: The study identified a novel function of PCK2 in mediating LPS-induced inflammation and provided mechanistic insights into the regulation of inflammatory responses in Kupffer cells. PCK2 overexpression significantly increased inflammation markers, while PCK2 knockdown or inhibition mitigated LPS-induced inflammatory response in Kupffer cells. Additionally, PCK2 promoted protein phosphorylation of NF-kappa B and AKT/MAPK, the major signaling pathways controlling inflammatory cascade activation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Peng Wang, Esra Karakose, Carmen Argmann, Huan Wang, Metodi Balev, Rachel Brody, Hembly G. Rivas, Xinyue Liu, Olivia Wood, Hongtao Liu, Lauryn Choleva, Dan Hasson, Emily Bernstein, Joao A. Paulo, Donald K. Scott, Luca Lambertini, James A. DeCaprio, Andrew F. Stewart
Summary: Resistance to regeneration of insulin-producing pancreatic β cells is a challenge in diabetes. Small molecule inhibitors of DYRK1A kinase have been found to induce proliferation of adult human β cells, but their mechanism of action is not fully understood. Through analysis of transcriptomic databases, researchers identified the DREAM complex as a central regulator of quiescence in human β cells. The DREAM complex consists of transcriptionally repressive proteins that assemble in response to DYRK1A kinase activity, inducing and maintaining cellular quiescence.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Karina Budkina, Krystel El Hage, Marie-Jeanne Clement, Benedicte Desforges, Ahmed Bouhss, Vandana Joshi, Alexandre Maucuer, Loic Hamon, Lev P. Ovchinnikov, Dmitry N. Lyabin, David Pastre
Summary: The study demonstrates how YB-1 unwinds mRNA secondary structures through specific structural mechanisms, facilitating SG disassembly and potentially inhibiting SG assembly in cancer cells.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Karina Budkina, Krystel El Hage, Marie-Jeanne Clement, Benedicte Desforges, Ahmed Bouhss, Vandana Joshi, Alexandre Maucuer, Loic Hamon, Lev P. Ovchinnikov, Dmitry N. Lyabin, David Pastre
Summary: The study reveals that YB-1 can unwind mRNA secondary structures under cellular stress by utilizing its cold-shock domain and C-terminal domain. YB-1 aids in stress granule disassembly during recovery, and its overexpression inhibits stress granule assembly in cancer cells by promoting mRNA translation initiation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Samantha S. Stadmiller, Gary J. Pielak
Summary: Biology is starting to understand the impact of crowded and complex intracellular environments on the thermodynamics and kinetics of protein folding, with a focus on protein-protein interactions. The study reviews experimental efforts to quantify how various molecules and cell interiors affect these interactions, and proposes integrating experiments and simulations for better understanding.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Federico Uliana, Rodolfo Ciuffa, Ranjan Mishra, Andrea Fossati, Fabian Frommelt, Sabrina Keller, Martin Mehnert, Eivind Salmorin Birkeland, Frank van Drogen, Nevena Srejic, Matthias Peter, Nicolas Tapon, Ruedi Aebersold, Matthias Gstaiger
Summary: This article describes an experimental strategy to investigate the interdependencies between protein phosphorylation and Protein-Protein Interactions (PPIs). The strategy involves systematically mapping the phosphorylation landscape of a target protein, assigning different proteoforms of the target protein to distinct protein complexes, and analyzing them in cells lacking regulators of the target protein. The study applied this strategy to YAP1 and identified multiple YAP1 phosphosites associated with distinct complexes, providing insights into their regulation by Hippo pathway members.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shiqiang Zhang, Xuan Zhang, Xin Guan, Xiaoli Ma, Hong Chen, Bingren Huang, Deng Chen
Summary: Phosphorylated YAF2 exhibits anti-apoptotic activity in human tumor cells by targeting FANK1 expression and inhibiting its degradation, leading to increased stability.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yu-Tze Horng, Novaria Sari Dewi Panjaitan, Yi-Jhen Tsai, Pin-Wei Su, Hung-Chi Yang, Po-Chi Soo
Summary: This study found that for most clinical isolates of Klebsiella pneumoniae, the amount of etcABC mRNA was positively related to their type 3 fimbriae production in a high level of glucose, thereby to their biofilm formation and colonization in the worm. However, in some isolates, MrkA was significantly reduced by high glucose levels.
Article
Biochemical Research Methods
Shin-Ichi Koda, Shinji Saito
Summary: The formation of the clock protein complex in cyanobacteria relies on the slow KaiB-KaiC interaction, which is promoted by inhibiting ADP/ATP exchange through accumulating ADP molecules. This inhibition not only accelerates complex formation, but also contributes to period robustness under environmental perturbations. Moreover, the stabilization of the binding-competent C1 by KaiB binding and relatively fast ADP/ATP exchange in the binding-incompetent C1 are essential for this simultaneous promotion and acceleration.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biology
Soichiro Suzuki, Fumiaki Ando, Sae Kitagawa, Yu Hara, Tamami Fujiki, Shintaro Mandai, Koichiro Susa, Takayasu Mori, Eisei Sohara, Tatemitsu Rai, Shinichi Uchida
Summary: Signaling through cAMP/protein kinase A (PKA) promotes endothelial barrier function to prevent plasma leakage. ZNF185, identified as a PKA substrate, interacts with PKA and colocalizes with F-actin in endothelial cells. Knockdown of ZNF185 disrupts actin filaments and promotes stress fiber formation, resulting in endothelial barrier dysfunction.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Lauren M. Hawkins, Anatoli V. Naumov, Mrinalini Batra, Changqi Wang, Dale Chaput, Elena S. Suvorova
Summary: This study investigates the process of endodyogeny in Toxoplasma gondii and discovers a novel parasite-specific complex, TgCrk6/TgCyc1, that regulates the spindle assembly checkpoint. The study demonstrates the involvement of parasite-specific tasks in the canonical checkpoint functions and provides insights into the unique replication mechanisms of Apicomplexa parasites.
Article
Biochemistry & Molecular Biology
Marta Delgado-Camprubi, Noemi Esteras, Marc P. M. Soutar, Helene Plun-Favreau, Andrey Y. Abramov
CELL DEATH AND DIFFERENTIATION
(2017)
Article
Pharmacology & Pharmacy
Holly Robertson, John D. Hayes, Calum Sutherland
BIOCHEMICAL PHARMACOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Claudia Manzoni, Adamantios Mamais, Sybille Dihanich, Marc P. M. Soutar, Helene Plun-Favreau, Rina Bandopadhyay, Rosella Abeti, Paola Giunti, John Hardy, Mark R. Cookson, Sharon A. Tooze, Patrick A. Lewis
BIOSCIENCE REPORTS
(2018)
Correction
Genetics & Heredity
Paul Jowsey, Nicholas A. Morrice, C. James Hastie, Hilary MacLauchlan, Rachel Toth, John Rouse
Article
Multidisciplinary Sciences
Marc P. M. Soutar, Liam Kempthorne, Shuichi Miyakawa, Emily Annuario, Daniela Melandri, Jasmine Harley, Gregory A. O'Sullivan, Selina Wray, David C. Hancock, Mark R. Cookson, Julian Downward, Mark Carlton, Helene Plun-Favreau
SCIENTIFIC REPORTS
(2018)
Editorial Material
Medicine, General & Internal
Christopher J. Schofield, Calum Sutherland
BRITISH JOURNAL OF HOSPITAL MEDICINE
(2019)
Letter
Cell Biology
Marc P. M. Soutar, Liam Kempthorne, Emily Annuario, Christin Luft, Selina Wray, Robin Ketteler, Marthe H. R. Ludtmann, Helene Plun-Favreau
Article
Genetics & Heredity
Lucia Schottlaender, Rosella Abeti, Zane Jaunmuktane, Carol Macmillan, Viorica Chelban, Benjamin O'Callaghan, John McKinley, Reza Maroofian, Stephanie Efthymiou, Alkyoni Athanasiou-Fragkouli, Raeburn Forbes, Marc P. M. Soutar, John H. Livingston, Bernardett Kalmar, Orlando Swayne, Gary Hotton, Alan Pittman, Joao Ricardo Mendes de Oliveira, Maria de Grandis, Angela Richard-Loendt, Francesca Launchbury, Juri Althonayan, Gavin McDonnell, Aisling Carr, Suliman Khan, Christian Beetz, Atil Bisgin, Sevcan Tug Bozdogan, Amber Begtrup, Erin Torti, Linda Greensmith, Paola Giunti, Patrick J. Morrison, Sebastian Brandner, Michel Aurrand-Lions, Henry Houlden
AMERICAN JOURNAL OF HUMAN GENETICS
(2020)
Article
Multidisciplinary Sciences
Changwei Chen, Jennifer R. Gallagher, Jamie Tarlton, Lidy van Aalten, Susan E. Bray, Michael L. J. Ashford, Rory J. McCrimmon, Ewan R. Pearson, Alison D. McNeilly, Calum Sutherland
Summary: Genotype may influence the therapeutic effects of metformin for type-2 diabetes, and the NPAT gene may play a role in the mechanism of metformin action.
Article
Biology
Francisco Bustos, Sunil Mathur, Carmen Espejo-Serrano, Rachel Toth, C. James Hastie, Satpal Virdee, Greg M. Findlay
Summary: This study utilized photocrosslinking activity-based probes to monitor the RING E3 ubiquitin ligase activity of RNF12 and demonstrated its application in assessing the impact of RNF12 variants on activity. The findings showed that the photoABPs accurately reported the effects of RNF12 variants on E3 activity, and the technology could be rapidly deployed in human pluripotent stem cells.
LIFE SCIENCE ALLIANCE
(2022)
Article
Clinical Neurology
Marc P. M. Soutar, Daniela Melandri, Benjamin O'Callaghan, Emily Annuario, Amy E. Monaghan, Natalie J. Welsh, Karishma D'Sa, Sebastian Guelfi, David Zhang, Alan Pittman, Daniah Trabzuni, Anouk H. A. Verboven, Kylie S. Pan, Demis A. Kia, Magda Bictash, Sonia Gandhi, Henry Houlden, Mark R. Cookson, Nael Nadif Kasri, Nicholas W. Wood, Andrew B. Singleton, John Hardy, Paul J. Whiting, Cornelis Blauwendraat, Alexander J. Whitworth, Claudia Manzoni, Mina Ryten, Patrick A. Lewis, Helene Plun-Favreau
Summary: Parkinson's disease is a common and incurable neurodegenerative disease. Genetic variants identified through genome-wide association studies have advanced our understanding of the disease's genetic risk. This study found that KANSL1 and KAT8 are regulators of PINK1-dependent mitophagy and may contribute to idiopathic Parkinson's disease.
Meeting Abstract
Biochemistry & Molecular Biology
L. V. Schottlaender, R. Abeti, Z. Jaunmuktane, C. Macmillan, V. Chelban, B. O'Callaghan, J. McKinley, R. Maroofian, S. Efthymiou, A. Fragkou, R. Forbes, M. Soutar, J. Livingston, B. Kalmar, O. Swayne, G. Hotton, A. Pittman, J. Mendes de Oliveira, M. De Grandis, A. Richard-Loendt, F. Launchbury, J. Althonayan, G. McDonnell, A. Carr, S. Khan, C. Beetz, A. Bisgin, S. Tug Bozdogan, A. Begtrup, E. Torti, L. Greensmith, P. Giunti, P. Morrison, S. Brandner, M. Aurrand-Lions, H. Houlden
EUROPEAN JOURNAL OF HUMAN GENETICS
(2020)
Article
Biochemical Research Methods
Virginia De Cesare, Jennifer Moran, Ryan Traynor, Axel Knebel, Maria Stella Ritorto, Matthias Trost, Hilary McLauchlan, C. James Hastie, Paul Davies
Meeting Abstract
Clinical Neurology
L. V. Schottlaender, R. Abeti, Z. Jaunmuktane, M. Soutar, J. Mckinley, O. Swayne, C. Bettencourt, R. Forbes, P. J. Morrison, D. Hughes, A. Pittman, B. Kalmar, M. de Grandis, G. V. McDonnell, S. Brandner, M. Aurrand Lyons, P. Giunti, H. Houlden
EUROPEAN JOURNAL OF NEUROLOGY
(2018)
Article
Biochemistry & Molecular Biology
Virginia De Cesare, Clare Johnson, Victoria Barlow, James Hastie, Axel Knebel, Matthias Trost
CELL CHEMICAL BIOLOGY
(2018)