期刊
BIOCHEMICAL SOCIETY TRANSACTIONS
卷 36, 期 -, 页码 754-757出版社
PORTLAND PRESS LTD
DOI: 10.1042/BST0360754
关键词
chromatin immunoprecipitation (ChIP); DNA binding; Escherichia coli; microarray; transcription
A major goal in the study of gene regulation is to untangle the transcription-regulatory networks of Escherichia coli and other 'simple' organisms. To do this we must catalogue the binding sites of all transcription factors. ChIP (chromatin immunoprecipitation), combined with DNA microarray analysis, is a powerful tool that permits global patterns of DNA binding to be measured. Here, we discuss the benefits of this approach and the application of this technique to bacterial systems.
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