Review
Cell Biology
Michael A. Hadders, Susanne M. A. Lens
Summary: The Chromosomal Passenger Complex (CPC) is a crucial protein complex that regulates various processes during nuclear and cytoplasmic division. It is recruited to centromeres and kinetochores in early mitosis to ensure proper connection of duplicated chromosomes to microtubules. During anaphase, the CPC is relocated to the microtubule overlaps and equatorial cortex, requiring direct interactions with a kinesin-6 family member and inactivation of cyclin B-CDK1.
TRENDS IN CELL BIOLOGY
(2022)
Article
Cell Biology
Song Yang, Youguang Luo, Mulin Yang, Hua Ni, Hanxiao Yin, Ming Hu, Min Liu, Jun Zhou, Yunfan Yang, Dengwen Li
Summary: Src is a key player in cell division, migration, adhesion, and survival. Its overactivation in tumors promotes cancer cell survival and mitosis. Src is conserved across species and its inhibition leads to abnormal expression of chromosomal passenger complex components, resulting in multipolar spindle formation and cell division failure. The findings suggest that Src inhibitors can be used as a novel approach for cancer treatment.
CELL AND TISSUE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xianghua Zhang, Ji Eun Park, Eun Ho Kim, Jihee Hong, Ki-Tae Hwang, Young A. Kim, Chang-Young Jang
Summary: The timely and temporal phosphorylation of proteins during mitosis is critical, with mitotic kinases activating and phosphatases repressing the process. The mitotic exit, or transition from mitosis to interphase, involves removal of mitotic phosphorylation by protein phosphatases, including PP1 and PP2A. A new mitotic phosphatase relay, involving Wip1/PPM1D phosphatase activity, is essential for CPC translocation in anaphase, demonstrating spatiotemporal regulation of mitotic exit to prevent tumor initiation and progression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yuri Prozzillo, Maria Virginia Santopietro, Giovanni Messina, Patrizio Dimitri
Summary: This review article focuses on the unconventional roles of epigenetic players, including chromatin remodelers and long non-coding RNAs, in cell division. The study highlights their impact on differentiation, development, and diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Cell Biology
Imge Ozugergin, Alisa Piekny
Summary: Cytokinesis, the process of physically dividing a cell into two daughters, has been extensively studied in vitro and early embryos, but its regulation in different animal cell types and developmental contexts remains poorly understood. Recent studies have revealed striking differences in the regulation of cytokinesis between different cell types and organisms, including diverse threshold requirements for structural components and different mechanisms of regulation. This review focuses on these differences, particularly in pathways independent of the mitotic spindle, and associated with the cortex, kinetochores, or chromatin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Toxicology
Liyan Xiao, Jinyan Pang, Hua Qin, Liyang Dou, Man Yang, Ji Wang, Xianqing Zhou, Yang Li, Junchao Duan, Zhiwei Sun
Summary: Through in vivo and in vitro experiments, it was found that amorphous silica nanoparticles can induce the formation of multinucleation. The study also revealed that amorphous silica nanoparticles inhibit the PI3k 110 & beta;/Aurora B pathway through excessive generation of reactive oxygen species, thereby hindering the clustering of the centralspindlin complex and the expression of related proteins, leading to cytokinesis failure and the formation of multinucleation.
PARTICLE AND FIBRE TOXICOLOGY
(2023)
Article
Cell Biology
Delaney Sherwin, Emily Gutierrez-Morton, Michael Bokros, Cory Haluska, Yanchang Wang
Summary: The conserved chromosomal passenger complex (CPC) corrects kinetochore attachment errors and prevents checkpoint silencing. Sli15 is phosphorylated by both CDK and Ipl1 kinase, and Cdc14 phosphatase reverses CDK-induced phosphorylation to promote CPC translocation. In addition, Fin1-PP1 also dephosphorylates Sli15 to enable CPC translocation. This pathway is crucial for accurate chromosome segregation.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Multidisciplinary Sciences
Jing Li, Shunya Ohmura, Aruna Marchetto, Martin F. Orth, Roland Imle, Marlene Dallmayer, Julian Musa, Maximilian M. L. Knott, Tilman L. B. Holting, Stefanie Stein, Cornelius M. Funk, Ana Sastre, Javier Alonso, Felix Bestvater, Merve Kasan, Laura Romero-Perez, Wolfgang Hartmann, Andreas Ranft, Ana Banito, Uta Dirksen, Thomas Kirchner, Florencia Cidre-Aranaz, Thomas G. P. Gruenewald
Summary: The study shows that targeting PRC1 or PLK1 can induce fatal genomic instability and tumor regression in EwS model. EWSR1-FLI1, an oncogenic transcription factor specific to EwS, hijacks PRC1 to promote tumor growth by binding to GGAA microsatellite. High PRC1 expression creates a therapeutic vulnerability towards PLK1 inhibition, leading to repression of even chemo-resistant EwS cells by triggering mitotic catastrophe.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Joao Barbosa, Claudio E. Sunkel, Carlos Conde
Summary: This review provides an overview of the molecular strategies that monitor and fine-tune KT-MT attachment formation during mitosis, to ensure accurate segregation of sister chromatids. This process is crucial for safe mitotic progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Charlotte S. Repton, C. Fiona P. Cullen, Mariana F. A. S. Costa, Christos P. Spanos, Juri S. Rappsilber, Hiroyuki P. Ohkura
Summary: This study reveals the important role of 14-3-3 protein in regulating multiple microtubule-associated proteins in fly oocytes. The interaction between 14-3-3 and one of the subunits is regulated by phosphorylations, which are crucial for localization and function of the subunit. As these proteins are conserved in many species, including humans, this study may provide insight into chromosome mis-segregation in human oocytes, which is a major cause of human infertility, miscarriages, and congenital birth conditions.
Article
Cell Biology
Eric M. C. Britigan, Jun Wan, Daniel K. Sam, Sarah E. Copeland, Amber L. Lasek, Laura C. F. Hrycyniak, Lei Wang, Anjon Audhya, Mark E. Burkard, Avtar Roopra, Beth A. Weaver
Summary: The increased expression of Aurora B protein reduces its kinase activity and causes defects in mitosis. The complexes of Aurora B and its binding partner INCENP achieve Aurora B activation through autophosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Eleni Persalaki, George Zachos
Summary: This study identified an ATM-Chk2-INCENP pathway that imposes the abscission checkpoint by regulating CPC midbody localization, preventing chromosome breakage or tetraploidization during cell division.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Shrividya Sana, Ashwathi Rajeevan, Sachin Kotak
Summary: The exclusive localization of NuMA and Ect2/Cyk4/Mklp1 ensures the coordination of spindle elongation and cleavage furrow formation. This coordination is achieved by restricting dynein/dynactin and RhoA to distinct membrane zones.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yasuhiro Kurasawa, Kyu Joon Lee, Huiqing Hu, Kieu T. M. Pham, Ziyin Li
Summary: The study demonstrates that TbPLK and TbAUK1 phosphorylate the cytokinesis regulators CIF1 and CIF2 in Trypanosoma brucei. TbPLK interacts with CIF1 in S/G2 phases, maintaining its localization until early mitosis; TbAUK1 maintains CIF1 and CIF2 localization from late mitosis.
Review
Biochemistry & Molecular Biology
Antal H. Kovacs, Dong Zhao, Jinqiang Hou
Summary: This paper presents a comprehensive review of the preclinical and clinical candidates of Aurora B inhibitors as potential anticancer drugs. The recent advances in the field of Aurora B inhibitor development will be highlighted, and the binding interactions between Aurora B and inhibitors based on crystal structures will be presented and discussed to provide insights for the future design of more selective Aurora B inhibitors.
