期刊
BIOCHEMICAL PHARMACOLOGY
卷 85, 期 12, 页码 1721-1726出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2013.04.016
关键词
Intestinal organoids; Embryonic stem cells; Induced pluripotent cells; Intestinal stem cells; High throughput screening
资金
- Connecticut Innovations
- NIH [R21CA158743]
Recently developed cell culture protocols have allowed for the derivation of multi-cellular structures dubbed intestinal organoids from embryonic stem cells (ESCs), induced pluripotent stem cells (IPSCs), and adult intestinal stem cells (ISCs). These structures resemble in vivo intestinal crypts, both in structure and developmental processes, and can be grown quickly and in relatively large quantities. Although much research has focused on developing intestinal organoids for tissue repair, more immediate applications include high-throughput screening for agents that target intestinal epithelium. Here we describe current methods for deriving mouse and human intestinal organoids and discuss some applications aimed at developing novel therapies or preventive agents for diseases of the lower GI tract such as inflammatory bowel diseases and colorectal cancer. (C) 2013 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据