Review
Pharmacology & Pharmacy
Jomon Sebastian, Krishnan Rathinasamy
Summary: Microtubules play a critical role in cell division and are a well-known target in cancer chemotherapy. Drugs that interfere with microtubule dynamics inhibit cell growth at the mitotic phase and induce cell death. Finding new microtubule inhibitors is important for overcoming the limitations of current drugs.
CURRENT DRUG TARGETS
(2023)
Article
Biochemistry & Molecular Biology
Mateja Mikulcic, Nassim Ghaffari Tabrizi-Wizsy, Eva M. Bernhart, Martin Asslaber, Christopher Trummer, Werner Windischhofer, Wolfgang Sattler, Ernst Malle, Andelko Hrzenjak
Summary: The study revealed that 15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) exhibits significant anti-tumor effects on osteosarcoma cells by promoting apoptosis, increasing intracellular ROS levels, and activating the MAPK pathway, offering a promising natural compound to interfere with osteosarcoma tumor growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Diane Pelzer, Ludmilla de Plater, Peta Bradbury, Adrien Eichmuller, Anne Bourdais, Guillaume Halet, Jean-Leon Maitre
Summary: Cell fragmentation is a common phenomenon in human preimplantation embryos and is associated with poor prognosis during assisted reproductive technology procedures. This study uses light sheet microscopy imaging to reveal that inefficient chromosome separation due to spindle defects, caused by dysfunctional molecular motors Myo1c or dynein, leads to cell fragmentation during mitosis. The extended exposure of the cell cortex to chromosomes triggers actomyosin contractility and pinches off cell fragments, similar to the process observed during meiosis. This study uncovers the mechanisms underlying cell fragmentation in preimplantation embryos and provides insight into the regulation of mitosis during the maternal-zygotic transition.
Review
Cell Biology
Abesh Bera, Mohan L. Gupta
Summary: Microtubules are dynamic polymers assembled from alpha- and beta-tubulin subunits, with diverse properties determined by different tubulin isotypes and posttranslational modifications. Research in microorganisms has provided valuable insights into the distinct properties of tubulin isotypes and their roles in facilitating specific cellular processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Maki Fujimoto, Hitoshi Higuchi, Yuka Honda-Wakasugi, Saki Miyake, Yukiko Nishioka, Akiko Yabuki-Kawase, Shigeru Maeda, Takuya Miyawaki
Summary: The study investigated the anti-inflammatory mechanism of dexmedetomidine in inhibiting LPS-induced inflammatory responses. It was found that dexmedetomidine inhibits inflammation by activating PPAR gamma or increasing the production of 15d-PGJ2. The results suggest that dexmedetomidine inhibits LPS-induced inflammatory responses by binding to alpha(2) adrenoceptors and activating PPAR gamma.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Cell Biology
Xiaxia Niu, Ting Wu, Qishuang Yin, Xinsheng Gu, Gege Li, Changlong Zhou, Mei Ma, Li Su, Shu Tang, Yanan Tian, Ming Yang, Hongmei Cui
Summary: This study found that the PXR antagonist SPA70 can reduce resistance to paclitaxel in non-small cell lung cancer (NSCLC). The combination of PTX and SPA70 may be a potential novel treatment for taxane-resistant lung cancer.
Article
Biochemistry & Molecular Biology
Li Chen, Anna Kashina
Summary: Arginylation, a posttranslational modification, is involved in the regulation of multiple biological processes. Studying its role in yeast, we found that arginylation is important for cell division, cell polarity, organelle transport, cytoskeleton organization and dynamics, and mitochondrial morphology and maintenance.
MOLECULAR AND CELLULAR BIOLOGY
(2022)
Article
Immunology
Victoria Lucia Alonso, Mara Emilia Carloni, Camila Silva Goncalves, Gonzalo Martinez Peralta, Maria Eugenia Chesta, Alejandro Pezza, Luis Emilio Tavernelli, Maria Cristina M. Motta, Esteban Serra
Summary: Trypanosomatids have a simpler cytoskeleton arrangement with stable microtubules, extensively acetylated in Trypanosoma cruzi. The identification of T. cruzi ATAT as a tubulin acetyltransferase sheds light on the role of alpha-tubulin acetylation in regulating cell cycle progression and parasite biology. Over-expression of TcATAT leads to morphological defects, kinetoplast division impairment, and increased resistance to microtubule depolymerizing drugs in T. cruzi, emphasizing the importance of finely regulated alpha-tubulin acetylation levels for normal cell cycle progression.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hannah J. Brown, Iain G. Duggin
Summary: Tubulin superfamily proteins play important roles in cellular functions such as morphology, division, and motility. The study of archaeal CetZ proteins reveals their diverse functions, including regulation of cell shape and potentially other unknown functions. The research also suggests the association of CetZ proteins among archaea and their possible origin via horizontal transfer. Furthermore, the study uncovers the relationship between CetZ proteins and functions related to the cell surface, biosynthesis, and motility.
Article
Biochemistry & Molecular Biology
Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Ching- Chang, Chuen-Mao Yang
Summary: 15d-PGJ(2) induces the expression of HO-1 in bEnd.3 cells through the activation of NOX- and mitochondria-derived ROS-dependent PKC delta/JNK1/2/Sp1/AP-1 signaling pathway, thus protecting against inflammatory responses.
Article
Microbiology
Francisco Alejandro Lagunas-Rangel, Janet Yee, Rosa Maria Bermudez-Cruz
Summary: Giardia duodenalis, a flagellated protozoan responsible for diarrheal disease worldwide, has a complex microtubule cytoskeleton and must coordinate cell division to ensure successful infection through duplication and partitioning of cell structures.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Marine Geoffroy, Marine Lemesle, Alexandra Kleinclauss, Sabine Mazerbourg, Levy Batista, Muriel Barberi-Heyob, Thierry Bastogne, Wilfrid Boireau, Alain Rouleau, Dorian Dupommier, Michel Boisbrun, Corinne Comoy, Stephane Flament, Isabelle Grillier-Vuissoz, Sandra Kuntz
Summary: The study demonstrated the potential of Troglitazone derivatives Delta 2-TGZ and AB186 in inducing apoptosis in breast cancer cells, with AB186 showing greater efficacy. AB186 was also shown to inhibit cell migration in triple negative breast cancer cells, suggesting it could be a promising candidate for developing novel therapeutic strategies for aggressive forms of breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Jingkai Zhou, Matthieu Corvaisier, Darina Malycheva, Maria Alvarado-Kristensson
Summary: Oncogenic transformation leads to adaptive changes in tumor cells, affecting their cellular organization and functions. These changes can be used as diagnostic markers in clinical settings. This review summarizes the roles of actin, microtubules, and the gamma-tubulin meshwork in regulating cell metabolism and functional cellular asymmetry.
Article
Cell Biology
Kristen Park, Katelyn J. Hoff, Linnea Wethekam, Nicholas Stence, Margarita Saenz, Jeffrey K. Moore
Summary: Mutations in beta-tubulin genes TUBB2A or TUBB3, specifically the T178M missense mutation, were found in two patients with brain malformations characteristic of tubulinopathies. The T178M mutation results in kinetically stabilized microtubules with decreased intrinsic assembly activity and fewer transitions to disassembly. This disruption of GTPase-dependent conformational changes in tubulin provides a mechanistic explanation for the kinetic stabilization observed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Jeremy Grignard, Veronique Lamamy, Eva Vermersch, Philippe Delagrange, Jean-Philippe Stephan, Thierry Dorval, Francois Fages
Summary: This study presents a mechanistic mathematical model of the microtubule tyrosination cycle, combining computational modeling and high-content image analyses. The model explains the inability to increase the tyrosination status by activating the Tubulin Tyrosine Ligase enzyme and predicts the necessity to combine two drugs to increase the tyrosination status in living cells.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Neurosciences
Jose A. Morales-Garcia, Sandra Alonso-Gil, Angel Santos, Ana Perez-Castillo
MOLECULAR NEUROBIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Angela Lopez-Sainz, Aitor Hernandez-Hernandez, Esther Gonzalez-Lopez, Fernando Dominguez, Maria Alejandra Restrepo-Cordoba, Marta Cobo-Marcos, Manuel Gomez-Bueno, Francisco Jose Hernandez-Perez, Juan Francisco Oteo, Jesus G. Mirelis, Miguel Angel Cavero, Vanessa Monivas, Susana Mingo Santos, F. Javier de Haro-del Moral, Isabel Krsnik, Clara Salas, Belen Bornstein, Ana Briceno, Juan Antonio Lopez, Jesus Vazquez, Luis Alonso-Pulpon, Javier Segovia, Pablo Garcia-Pavia
Summary: Cardiac amyloidosis is a disease caused by amyloid fiber deposition in the myocardium, with different subtypes leading to varied presentation and outcomes. Early diagnosis and treatment improvements are crucial in reducing mortality for these patients.
REVISTA ESPANOLA DE CARDIOLOGIA
(2021)
Article
Biochemical Research Methods
Nerea Corbacho-Alonso, Montserrat Baldan-Martin, Juan Antonio Lopez, Elena Rodriguez Sanchez, Paula J. Martinez, Laura Mourino-Alvarez, Tatiana Martin-Rojas, Tamara Sastre-Oliva, Felipe Madruga, Jesus Vazquez, Luis R. Padial, Gloria Alvarez-Llamas, Fernando Vivanco, Gema Ruiz-Hurtado, Luis M. Ruilope, Maria G. Barderas
JOURNAL OF PROTEOMICS
(2020)
Article
Cell Biology
Dorian Forte, Maria Garcia-Fernandez, Abel Sanchez-Aguilera, Vaia Stavropoulou, Claire Fielding, Daniel Martin-Perez, Juan Antonio Lopez, Ana S. H. Costa, Laura Tronci, Efterpi Nikitopoulou, Michael Barber, Paolo Gallipoli, Ludovica Marando, Carlos Lopez Fernandez de Castillejo, Alexandar Tzankov, Sabine Dietmann, Michele Cavo, Lucia Catani, Antonio Curti, Jesus Vazquez, Christian Frezza, Brian J. Huntly, Juerg Schwaller, Simon Mendez-Ferrer
Article
Cell Biology
Lucas Albacete-Albacete, Inmaculada Navarro-Lerida, Juan Antonio Lopez, Ines Martin-Padura, Alma M. Astudillo, Alessia Ferrarini, Michael Van-der-Heyden, Jesus Balsinde, Gertraud Orend, Jesus Vazquez, Miguel Angel del Pozo
JOURNAL OF CELL BIOLOGY
(2020)
Article
Health Care Sciences & Services
Tatiana Martin-Rojas, Tamara Sastre-Oliva, Ana Esclarin-Ruz, Felix Gil-Dones, Laura Mourino-Alvarez, Nerea Corbacho-Alonso, Rafael Moreno-Luna, German Hernandez-Fernandez, Juan Antonio Lopez, Antonio Oliviero, Maria G. Barderas
JOURNAL OF PERSONALIZED MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Nerea Corbacho-Alonso, Montserrat Baldan-Martin, Juan Antonio Lopez, Elena Rodriguez-Sanchez, Paula J. Martinez, Laura Mourino-Alvarez, Tamara Sastre-Oliva, Martha Cabrera, Eva Calvo, Luis R. Padial, Jesus Vazquez, Fernando Vivanco, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, Maria G. Barderas
Summary: This study investigates cardiovascular risk stratification in young adults based on oxidative stress and reveals that oxidation is progressive, with certain proteins differentially expressed between groups and involved in antioxidant response. The identification of redox targets and quantification of oxidative changes could help better understand the role of oxidative stress in cardiovascular disease and improve stratification for cardiovascular events.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Peripheral Vascular Disease
Aranzazu Santiago-Hernandez, Marta Martin-Lorenzo, Paula J. Martinez, Maria Gomez-Serrano, Juan Antonio Lopez, Pablo Cannata, Vanesa Esteban, Angeles Heredero, Gonzalo Aldamiz-Echevarria, Jesus Vazquez, Gema Ruiz-Hurtado, Maria G. Barderas, Julian Segura, Luis M. Ruilope, Gloria Alvarez-Llamas
Summary: This study aimed to identify early molecular alterations behind albuminuria development, uncovering important protein changes associated with high-normal albuminuria, and confirming their potential to reflect subclinical organ damage. Protein variations persisted and worsened in progressors to moderately increased albuminuria after 12 months.
JOURNAL OF HYPERTENSION
(2021)
Article
Immunology
Alma Yuste-Montalvo, Sergio Fernandez-Bravo, Tamara Oliva, Carlos Pastor-Vargas, Diana Betancor, Maria Jose Goikoetxea, Jose Julio Laguna, Juan Antonio Lopez, Gloria Alvarez-Llamas, Javier Cuesta-Herranz, Marta Martin-Lorenzo, Vanesa Esteban
Summary: The study analyzed the protein expression of human microvascular endothelial cells in response to sera from anaphylactic patients and non-allergic subjects. The findings revealed significant alterations in the proteome of the EC-anaphylaxis system, particularly in proteins related to the coagulation and complement systems. This comprehensive analysis provides insights into the pathophysiological mechanisms and identifies new target proteins and signaling pathways in anaphylactic reactions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Guillermo Albericio, Susana Aguilar, Jose Luis Toran, Rosa Yanez, Juan Antonio Lopez, Jesus Vazquez, Carmen Mora, Antonio Bernad
Summary: This study investigated the protein composition of cardiac progenitor cells (CPC) and found that they exhibit hypoxia response capacity and active collagen metabolism. The study also identified specific proteins that are significantly regulated in the nuclear compartment of CPC compared to human mesenchymal stem cells (hMSC). Two proteins, IL1A and IMP3, were found to be upregulated in both nuclear and mRNA expression, and further investigation revealed their potential roles in apoptosis regulation and proliferation/migration regulation, respectively.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Ayelen M. Santamans, Valle Montalvo-Romeral, Alfonso Mora, Juan Antonio Lopez, Francisco Gonzalez-Romero, Daniel Jimenez-Blasco, Elena Rodriguez, Aranzazu Pintor-Chocano, Cristina Casanueva-Benitez, Rebeca Acin-Perez, Luis Leiva-Vega, Jordi Duran, Joan J. Guinovart, Jesus Jimenez-Borreguero, Jose Antonio Enriquez, Maria Villlalba-Orero, Juan P. Bolanos, Patricia Aspichueta, Jesus Vazquez, Barbara Gonzalez-Teran, Guadalupe Sabio
Summary: During the early postnatal heart development, activation of the p38 gamma and p38 delta protein kinases leads to a metabolic shift in the heart, potentially inhibiting glycogen synthase and reducing glycogen production. Premature activation of these kinases may result in inadequate cardiac fuel supply and systemic metabolic disruption.
Article
Biochemistry & Molecular Biology
Tamara Sastre-Oliva, Nerea Corbacho-Alonso, Diego Albo-Escalona, Juan A. Lopez, Luis F. Lopez-Almodovar, Jesus Vazquez, Luis R. Padial, Laura Mourino-Alvarez, Maria G. Barderas
Summary: This study found that coronary artery disease affects valve calcification and modifies the molecular profile of aortic valve tissue. These findings are important for understanding the relationship between calcific aortic valve disease and coronary artery disease.
Article
Multidisciplinary Sciences
Carla Sanchez-Castillo, Maria Cuartero, Alba Fernandez-Rodrigo, Victor Briz, Sergio Lopez-Garcia, Raquel Jimenez-Sanchez, Juan A. Lopez, Mariona Graupera, Jose A. Esteban
Summary: Neuronal connectivity and synaptic plasticity are essential for brain function. Phosphatidylinositol 3-kinase (PI3K) signaling plays a crucial role in neuronal growth and synaptic plasticity. This study demonstrates that the two major isoforms of PI3K, p110 alpha and p110 beta, have distinct functions in dendritic and postsynaptic structures, neurotransmitter release, and long-term potentiation.
Article
Chemistry, Medicinal
Nuria Vilaboa, Juan Antonio Lopez, Marco de Mesa, Clara Escudero-Duch, Natalie Winfield, Melanie Bayford, Richard Voellmy
Summary: Exposure to celastrol, withaferin A, and synthetic compounds caused denaturation of luciferase reporter protein in cancer cells, leading to proteostasis disruption. Proteomic analysis revealed covalent modification of numerous cellular proteins targeted by the compounds. Important fractions of these proteins unfolded and formed aggregates. Multiple myeloma cells were particularly sensitive to these compounds. Development of a new proteostasis-disrupting therapy for multiple myeloma is suggested.
Article
Cell Biology
Susana Aguilar, Paula Garcia-Olloqui, Lidia Amigo-Moran, Jose Luis Toran, Juan Antonio Lopez, Guillermo Albericio, Gloria Abizanda, Diego Herrero, Africa Vales, Saray Rodriguez-Diaz, Marina Higuera, Ruben Garcia-Martin, Jesus Vazquez, Carmen Mora, Gloria Gonzalez-Aseguinolaza, Felipe Prosper, Beatriz Pelacho, Antonio Bernad
Summary: Oxidative stress-induced myocardial apoptosis and necrosis play a critical role in ischemic infarction. This study identified and validated miR-935 as a highly differentially expressed miRNA in exosomes derived from human cardiac progenitor cells (CPC). Reduction of miR-935 expression promotes oxidative stress-associated apoptosis. Together with other exosomal miRNA members, miR-935 may counteract oxidative stress-related apoptosis in the CPC microenvironment.
Article
Pharmacology & Pharmacy
Paola Orlandi, Marta Banchi, Francesca Vaglini, Marco Carli, Stefano Aringhieri, Arianna Bandini, Carla Pardini, Cristina Viaggi, Michele Lai, Greta Ali, Alessandra Ottani, Eleonora Vandini, Patrizia Guidi, Margherita Bernardeschi, Veronica La Rocca, Giulio Francia, Gabriella Fontanini, Mauro Pistello, Giada Frenzilli, Daniela Giuliani, Marco Scarselli, Guido Bocci
Summary: This study investigates the role of MC4R in melanoma and the use of the selective antagonist ML in combination with vemurafenib. The results show that ML can inhibit melanoma cell proliferation and induce apoptosis through the inhibition of ERK1/2 phosphorylation and reduction of BCL-XL expression. The combination of vemurafenib and ML exhibits a synergistic effect in vitro and inhibits tumor growth in vivo without causing adverse effects.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Conor J. Bloxham, Katina D. Hulme, Fabrizio Fierro, Christian Fercher, Cassandra L. Pegg, Shannon L. O'Brien, Simon R. Foster, Kirsty R. Short, Sebastian G. B. Furness, Melissa E. Reichelt, Masha Y. Niv, Walter G. Thomas
Summary: Bitter taste receptors (T2Rs) are a type of G protein-coupled receptors that allow humans to detect aversive and toxic substances. This study characterized the functional properties of previously identified T2Rs in human cardiac tissues and their naturally occurring polymorphisms. The results showed differences in signaling among different T2R variants, and revealed a potential association between the T2R50 Tyr203 variant and cardiovascular disease.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Lu Chen, Huanying Shi, Wenxin Zhang, Yongjun Zhu, Haifei Chen, Zimei Wu, Huijie Qi, Jiafeng Liu, Mingkang Zhong, Xiaojin Shi, Tianxiao Wang, Qunyi Li
Summary: This study demonstrates that Carfilzomib exhibits potent anti-tumor activity against esophageal squamous cell carcinoma (ESCC) by triggering mitochondrial apoptosis and reprogramming cellular metabolism. It has been identified that activating transcription factor 3 (ATF3) plays a crucial role as a cellular target in ESCC cells treated with Carfilzomib. Overexpression of ATF3 effectively counteracts the effects of Carfilzomib on ESCC cell proliferation, apoptosis, and metabolic reprogramming. Furthermore, ATF3 mediates the anti-tumor activity of Carfilzomib, suggesting its potential as a therapeutic agent for ESCC.
BIOCHEMICAL PHARMACOLOGY
(2024)
Review
Pharmacology & Pharmacy
Xing Zhang, Xiang Li, Ran Xia, Hong-Sheng Zhang
Summary: This review summarizes recent progress on the mechanisms of ferroptosis resistance in cancer and highlights the role of redox status and metabolism. Combination therapy for ferroptosis has great potential in treating resistant malignant tumors.
BIOCHEMICAL PHARMACOLOGY
(2024)