期刊
BIOCHEMICAL PHARMACOLOGY
卷 75, 期 11, 页码 2204-2213出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2008.02.030
关键词
VDR; RXR; retinoid; CYP3A4
资金
- NATIONAL CANCER INSTITUTE [R01CA053596, R29CA053596] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR021940] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM [R01AA014147] Funding Source: NIH RePORTER
- NCI NIH HHS [R01 CA053596-11, R01 CA053596-13, R01 CA053596-15, R01 CA053596-09, R01 CA053596, R01 CA053596-10, R01 CA053596-16, R01 CA053596-14, CA53596, R01 CA053596-18, R01 CA053596-12, R01 CA053596-17] Funding Source: Medline
- NCRR NIH HHS [P20 RR021940] Funding Source: Medline
- NIAAA NIH HHS [R01 AA014147-03, R01 AA014147-01, R01 AA014147-02, R01 AA014147, AA14147, R01 AA014147-04, R01 AA014147-05] Funding Source: Medline
A panel of retinoids and carotenoids was screened as potential inducers of CYP3A4 through the RXR/VDR-mediated signaling pathway. Transient transfection. assays revealed that 3 out of 12 retinoids screened transactivated RXR alpha/VDR and induced CYP3A4 reporter activity. These three retinoids are the active metabolites of retinoids, 9-cis-retinal, 9-cis-retinoic acid (9-cis-RA), and all-trans-retinoic acid (all-trans-RA). 9-cis-RA and all-trans-RA preferentially transactivated the RXR/VDR heterodimers and RXR homodimers. Retinoids and VDR agonist 1 alpha, 25-dihydroxyvitamin D-3, but not PXR or CAR activator, could induce Cyp3a11 mRNA level in hepatocytes derived from PXR/CAR-double null mouse. Moreover, retinoids induced CYP3A4 enzyme activity in HepG2 human hepatoma and Caco-2 human colorectal adenocarcinoma cells. A direct role of retinoid-mediated CYP3A4 induction through RXRa/VDR was proved by the results that 9-cis-retinal, 9-cis-RA, and all-trans-RA recruited RXR alpha and VDR to CYP3A4 regulatory region pER6 (proximal everted repeat with a 6-nucleotide spacer) and dXREM (distal xenobiotic-responsive enhancer module). Thus, using various approaches, we have unequivocally demonstrated that retinoids transactivate RXR/VDR heterodimers and RXR homodimers and induce CYP3A expression at mRNA as well as enzyme activity levels in both liver and intestinal cells. It is possible that retinoids might alter endobiotic metabolism through CYP3A4 induction in vivo. (C) 2008 Elsevier Inc. All rights reserved.
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