Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Article
Endocrinology & Metabolism
Petrina Koumarianou, Celia Fernandez-Mendez, Danae Fajardo-Delgado, Lidia Mirella Mielu, Pilar Santisteban, Antonio De la Vieja
Summary: This study reveals the involvement of the clathrin-coated machinery in basolateral trafficking of NIS (sodium/iodide symporter), providing further understanding of NIS subcellular sorting mechanisms. These findings will help uncover new targets in thyroid cancer treatment.
Article
Multidisciplinary Sciences
Nathan M. Willy, Federico Colombo, Scott Huber, Anna C. Smith, Erienne G. Norton, Comert Kural, Emanuele Cocucci
Summary: The research demonstrates the important role of CALM in the formation of clathrin-coated vesicles, showing that an increase in its level promotes vesicle completion. Variations in adaptor protein expression levels among different cells support the hypothesis that the efficiency of clathrin-mediated endocytosis is tissue-specific.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Wei Siao, Peng Wang, Xiuyang Zhao, Lam Dai Vu, Ive De Smet, Eugenia Russinova
Summary: Phosphorylation of the small subunit of ADAPTOR PROTEIN-2 by ADAPTOR-ASSOCIATED PROTEIN KINASE1 has an impact on Arabidopsis root tropic growth. AAK1 is involved in clathrin-mediated endocytosis regulation in mammals by phosphorylating the μ2 subunit of the AP-2 complex. Previous research found an association between AAK1 and AP-2 in Arabidopsis, but its function was unclear. In this study, genetic analysis revealed that both aak1 and ap2m mutants in Arabidopsis exhibit altered root tropic growth, including impaired touch and gravity responses. Phosphorylation of AP2M on Thr-163 by AAK1 fine-tunes endocytosis in the Arabidopsis root to control tropic growth.
Article
Biology
Tania Lopez-Hernandez, Koh-Ichiro Takenaka, Yasunori Mori, Pornparn Kongpracha, Shushi Nagamori, Volker Haucke, Shigeo Takamori
Summary: This study found that the endocytosis of synaptic vesicles in mouse hippocampal neurons at physiological temperature is independent of clathrin, while the endocytic retrieval of certain synaptic vesicle proteins, including vesicular transporters for glutamate and GABA, relies on sorting by the clathrin adaptor AP-2.
Article
Biochemistry & Molecular Biology
Carlos Anton-Plagaro, Noelia Sanchez, Rosario Valle, Jose Miguel Mulet, Mara C. Duncan, Cesar Roncero
Summary: Protein sorting at the trans-Golgi network (TGN) requires the assistance of cargo adaptors. The exomer complex in Saccharomyces cerevisiae plays a significant role in protein sorting, and has an unresolved functional relationship with TGN clathrin adaptors.
Article
Developmental Biology
Lisa Redlingshofer, Frances M. Brodsky
Summary: The AP2 adaptor complex plays a central role in regulating clathrin-mediated endocytosis, serving as a key hub for protein interactions beyond cargo recognition and clathrin recruitment. It mediates clathrin coated pit maturation and couples lattice formation to membrane deformation. AP2 complements the attenuating role of clathrin light chain subunits in driving clathrin assembly and dynamic lattice rearrangement for budding.
CELLS & DEVELOPMENT
(2021)
Article
Cell Biology
Miguel Ramirez Moreno, Katy Boswell, Helen L. Casbolt, Natalia A. Bulgakova
Summary: Intracellular trafficking, regulated by the AP-1 complex, plays a crucial role in epithelial morphogenesis and cell adhesion. Knockdown of AP-1 leads to tissue folding and cell death, potentially acting as a tumor suppressor. Furthermore, E-cadherin hyperinternalization and increased expression contribute to cell-cell adhesion maintenance.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Plant Sciences
Yao Yao, Jihua Zhou, Can Cheng, Fuan Niu, Anpeng Zhang, Bin Sun, Rongjian Tu, Jianing Wan, Yao Li, Yiwen Huang, Kaizhen Xie, Yuting Dai, Hui Zhang, Jing Han Hong, Xiaohua Pan, Jiaojiao Zhu, Hong Zhou, Zhenhua Liu, Liming Cao, Huangwei Chu
Summary: Clathrin-mediated vesicle trafficking (CMVT) is a fundamental process in all eukaryotic species, playing crucial roles in growth and development. Mutation of OsSCYL2 in rice results in light-dependent HR-like cell death and enhanced resistance to bacterial pathogens, revealing a novel component in the regulation of plant immunity.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Cell Biology
Shimin Wang, Longfeng Yao, Wenjuan Zhang, Zihang Cheng, Can Hu, Hang Liu, Yanling Yan, Anbing Shi
Summary: The deficiency of SMAP-1 leads to mislocalization of apical and basolateral proteins on the trans-Golgi network (TGN), with SMAP-1 colocalizing with AP-1. The AP-1 complex regulates the TGN localization of SMAP-1, promoting the assembly of clathrin to ensure polarized conventional secretion in C. elegans intestinal epithelia.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Hongfei Xu, Juan A. Oses-Prieto, Mikhail Khvotchev, Shweta Jain, Jocelyn Liang, Alma Burlingame, Robert H. Edwards
Summary: This study reveals that a subset of synaptic vesicles made by adaptor protein AP-3 enables release of neurotransmitters at high frequency. This release is facilitated by the localization of phospholipid flippase ATP8A1 onto the vesicles, which recruits synapsin for high frequency release.
NATURE NEUROSCIENCE
(2023)
Article
Oncology
Raga Ishikawa, Shosuke Yoshida, Shin-ichi Sawada, Yoshihiro Sasaki, Kazunari Akiyoshi
Summary: A method using cell-derived lipid membrane capsules called extracellular vesicles (EVs) was developed to deliver a model cargo into the cytosol. These hybrid EVs, formed by fusing EVs with liposomes, showed potential as drug delivery carriers by allowing cargo molecules to be more easily encapsulated and released into the cytosol. The engineered hybrid EVs were internalized by cells and fused with acidic organelles, releasing the cargo into the cytosol.
Review
Chemistry, Physical
Hassiba Smida, Christine Thobie-Gautier, Mohammed Boujtita, Estelle Lebegue
Summary: Single liposome electrochemistry is a sensitive and versatile tool for understanding biological processes and mechanisms at the lipid membrane/electrode interface. It offers the opportunity to detect various biotargets with high sensitivity in a short time, but it also has limitations.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Plant Sciences
Pallavi V. Vetal, Yves Poirier
Summary: Inorganic phosphate (Pi) homeostasis is crucial for plant growth and relies on the transport of Pi across plant cells. A protein called PHO1 plays a role in this transport process, but its localization on the plasma membrane does not align with its function. By inhibiting a process called clathrin-mediated endocytosis (CME), researchers discovered that PHO1 can be re-localized to the plasma membrane, which affects its transport function.
Article
Multidisciplinary Sciences
Yang Chen, Shaoqin Hu, Xuanang Wu, Zhenli Xie, Yuan Wang, Bianbian Wang, Xiaopeng Li, Yingmei Pei, Yuhao Gu, Kai Huang, Jingxiao Huo, Anqi Wei, Cheng Bi, Zhe Lu, Qian Song, Huadong Xu, Xinjiang Kang, Shuli Shao, Jiangang Long, Jiankang Liu, Zhuan Zhou, Rong Huang, Zuying Chai, Changhe Wang
Summary: Exocytosis and endocytosis are tightly coupled processes. The primary Ca2+ sensor Syt1 plays bidirectional roles in this coupling by promoting small-sized endocytosis but inhibiting large-sized bulk endocytosis. Ca2+ binding ability is crucial for Syt1 to regulate both types of endocytic pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Tina Junne, Martin Spiess
JOURNAL OF CELL SCIENCE
(2017)
Article
Multidisciplinary Sciences
Dominik P. Buser, Kai D. Schleicher, Cristina Prescianotto-Baschong, Martin Spiess
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Review
Biochemistry & Molecular Biology
Martin Spiess, Tina Junne, Marco Janoschke
Article
Multidisciplinary Sciences
Dominik P. Buser, Martin Spiess
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Medicine, General & Internal
Dominik P. Buser, Marie-Francoise Ritz, Suzette Moes, Cristobal Tostado, Stephan Frank, Martin Spiess, Luigi Mariani, Paul Jeno, Jean-Louis Boulay, Gregor Hutter
Review
Cell Biology
Martin Spiess, Michael Friberg, Nicole Beuret, Cristina Prescianotto-Baschong, Jonas Rutishauser
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Sung-jun Jung, Ji Eun Hani Kim, Tina Junne, Martin Spiess, Hyun Kim
Summary: This study investigated the targeting and topogenesis of the signal-anchored protein Spc3, finding that it reaches its final topology in two steps: partially dependent on SRP and then mediated by the Sec62/Sec63 complex.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Marco Janoschke, Mirjam Zimmermann, Anna Brunauer, Raffael Humbel, Tina Junne, Martin Spiess
Summary: The topology of membrane proteins is defined by the integration of alpha-helical transmembrane domains at the Sec61 translocon. Different sequences preceding a potential transmembrane domain significantly affect the hydrophobicity requirement for integration, with rapidly folding domains and strong chaperone binding facilitating efficient transmembrane integration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Editorial Material
Cell Biology
Valentina Millarte, Martin Spiess
Summary: In this recent study, a new role for RABEP1/Rabaptin5 in targeting the autophagy machinery to damaged early endosomes was uncovered. This was achieved through its interaction with RB1CC1/FIP200 and ATG16L1, and sheds light on the specific autophagy of damaged early endosomes.
Article
Biochemistry & Molecular Biology
Valentina Millarte, Simon Schlienger, Simone Kaelin, Martin Spiess
Summary: Selective autophagy of damaged organelles is essential for cellular homeostasis. Rabaptin5 interacts with FIP200 and ATG16L1 to mediate autophagy of damaged early endosomes and contributes to the elimination of Salmonella enterica after infection.
Article
Cell Biology
Mirjam Pennauer, Katarzyna Buczak, Cristina Prescianotto-Baschong, Martin Spiess
Summary: ADP-ribosylation factors (Arfs) play a crucial role in regulating membrane traffic in the secretory pathway, and the functional specificity and redundancy of different Arfs vary.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Jennifer Reck, Nicole Beuret, Erhan Demirci, Cristina Prescianotto-Baschong, Martin Spiess
Summary: Unlike constitutively secreted proteins, peptide hormones are stored in secretory granules and released upon stimulation. Small disulfide loops present in hormone precursors can act as signals for granule formation and aggregation. These findings suggest a novel mechanism for the sorting of peptide hormones into secretory granules.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biology
Dominik P. Buser, Gaetan Bader, Martin Spiess
Summary: Retrograde protein transport plays a crucial role in maintaining membrane homeostasis and recycling of mannose-6-phosphate receptors (MPRs) for sorting of lysosomal hydrolases. Using a sulfation tool based on nanobodies, we directly determined the transport kinetics of cation-dependent MPR (CDMPR) from the plasma membrane to the trans-Golgi network (TGN). Our study suggests a potential role of GGA adaptors in retrograde and anterograde transport.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biochemistry & Molecular Biology
Samuel Itskanov, Laurie Wang, Tina Junne, Rumi Sherriff, Li Xiao, Nicolas Blanchard, Wei Q. Shi, Craig Forsyth, Dominic Hoepfner, Martin Spiess, Eunyong Park
Summary: The Sec61 complex forms a protein-conducting channel in the endoplasmic reticulum membrane that is essential for protein secretion. Various small molecules have been identified as specific inhibitors of Sec61, but their inhibitory mechanisms are not well understood. This study presents detailed structures of human Sec61 bound to a variety of small molecule inhibitors, revealing a common binding pocket that stabilizes the closed state of the protein-translocation pore. These findings provide valuable insights for further pharmacological studies and drug design targeting Sec61.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biology
Nicole Beuret, Franziska Hasler, Cristina Prescianotto-Baschong, Julia Birk, Jonas Rutishauser, Martin Spiess