Review
Plant Sciences
Aline Koch, Michael Wassenegger
Summary: Host-induced gene silencing (HIGS) technology is a powerful alternative for plant protection, but its efficacy and specificity require more fundamental knowledge on molecular mechanisms. Contributions of host and target RNA interference machineries may be incompatible, and closing knowledge gaps will lead to novel concepts, precise risk assessment, and tailor-made HIGS therapy for plant diseases.
Article
Oncology
Kuebra Kaban, Clemens Hinterleitner, Yanjun Zhou, Emine Salva, Ayse Gulten Kantarci, Helmut R. Salih, Melanie Maerklin
Summary: The overexpression of antiapoptotic protein BCL-2 is linked with ER expression in breast cancer, and a novel treatment strategy using NK cell-derived exosomes as carriers for BCL-2 targeted siRNAs has been proposed. This approach has shown to enhance the killing ability of NK cell-derived exosomes by silencing BCL-2 overexpression and targeting oncogenes in breast cancer.
Review
Pharmacology & Pharmacy
Lei Zhang, Yayu Liang, Guohui Liang, Zhili Tian, Yue Zhang, Zhihui Liu, Xinying Ji
Summary: This article introduces RNA interference technology and its application in genetic therapy, focusing on the advantages and limitations of GalNAc-siRNA conjugates and lipid nanoparticles as delivery systems, and looking forward to the development of oligonucleotide drugs in genetic therapy.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Masuma Akter Monty, Md. Ariful Islam, Xu Nan, Jingwen Tan, Israth Jahan Tuhin, Xiaowen Tang, Miao Miao, Depei Wu, Lei Yu
Summary: RNAi effectors have the potential to modulate the immune response in cancer treatment by down-regulating immune-suppressive proteins, leading to increased anti-tumour immunity.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Ying Liu, Xin Wang, Hanjun Chen, Tingting Wu, Yu Cao, Zhihong Liu
Summary: Chemodynamic therapy (CDT) is a promising strategy for cancer treatment, but its efficiency is limited by insufficient intracellular H2O2 level. This study presents a method to enhance CDT by silencing the CAT gene to accumulate H2O2 in tumor cells. Cu-siRNA nanocomposites are fabricated and modified with HA for active tumor targeting. Cu+ is generated by reduction of released Cu2+ by GSH, which then catalyzes H2O2 to produce toxic hydroxyl radicals to kill tumor cells. CAT siRNA efficiently inhibits H2O2 consumption, leading to H2O2 accumulation. Cu-siRNA@ HA exhibits good biocompatibility and therapeutic efficiency, offering a new way to supply H2O2 in CDT with potential for clinical application.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Medicine, Research & Experimental
Parisa Lotfinejad, Tohid Kazemi, Sahar Safaei, Mohammad Amini, Elmira Roshani Asl, Elham Baghbani, Siamak Sandoghchian Shotorbani, Farhad Jadidi Niaragh, Afshin Derakhshani, Mahdi Abdoli Shadbad, Nicola Silvestris, Behzad Baradaran
Summary: TNBC with high PD-L1 expression can induce an immunosuppressive tumor microenvironment, but targeting PD-L1 through siRNA can reduce its expression, decrease cancer cell proliferation, induce apoptosis, and effectively inhibit cell migration.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Xueli Zhang, Amu Gubu, Jianfei Xu, Ning Yan, Wenbo Su, Di Feng, Qian Wang, Xinjing Tang
Summary: A new method for the bio-orthogonal chemical activation of siRNA based on tetrazine-induced bond-cleavage reaction was developed, successfully achieving conditional gene silencing and providing a new uncaging strategy with small molecules.
Article
Plant Sciences
Federico Betti, Maria Jose Ladera-Carmona, Daan A. Weits, Gianmarco Ferri, Sergio Iacopino, Giacomo Novi, Benedetta Svezia, Alicja B. Kunkowska, Antonietta Santaniello, Alberto Piaggesi, Elena Loreti, Pierdomenico Perata
Summary: Plants can uptake exogenous RNA to affect gene expression in nearby plants via RNAi mechanism, indicating a role of miRNA as signaling molecules for plant-to-plant communication. This communication mechanism requires the production of secondary siRNAs.
Article
Chemistry, Multidisciplinary
Huan Zhang, Yuhong Cao, Dawei Xu, Natalie S. Goh, Gozde S. Demirer, Stefano Cestellos-Blanco, Yuan Chen, Markita P. Landry, Peidong Yang
Summary: The study synthesizes PEI-AuNCs for delivering siRNA into intact plants, achieving efficient gene knockdown without being hindered by the plant cell wall, providing a new approach for broad applications in plant biotechnology.
Article
Pharmacology & Pharmacy
Adhika Balgobind, Aliscia Daniels, Mario Ariatti, Moganavelli Singh
Summary: This study assessed the efficacy of cationic lipid-based systems for the delivery of HER2/neu siRNA in breast cancer. The results showed that cationic liposomes efficiently bound, compacted, and protected the therapeutic siRNA against degradation. The non-PEGylated Chol-T-siRNA lipoplexes exhibited the highest HER2/neu silencing.
Article
Biochemistry & Molecular Biology
Xialan Dong, Weifan Zheng
Summary: In designing effective siRNAs for a specific mRNA target, having predictive models for potency is crucial. A new approach using BCUT descriptors to characterize nucleotides can successfully predict gene silencing potency by cm-siRNAs, achieving comparable or superior results to existing algorithms. The method can model gene silencing potency for both natural and chemically modified siRNA molecules.
Article
Biotechnology & Applied Microbiology
Wenhua Bao, Aoga Li, Yanan Zhang, Pengfei Diao, Qiqi Zhao, Ting Yan, Zikai Zhou, Huimin Duan, Xugang Li, Hada Wuriyanghan
Summary: The study introduces Host-induced gene silencing (HIGS) as a new strategy for pest control and demonstrates the successful application of transgenic plants targeting chitinase encoding genes in the maize pest Mythimna separata. The development of an artificial microRNA (amiR) based PTA system has significantly improved knockdown efficiency and caused more pronounced developmental abnormalities in recipient insects. Common features and differences in siRNA characteristics after HIGS and direct dsRNA/siRNA feeding are analyzed, along with the identification of core RNAi genes in M. separata. The study provides compelling evidence for the potential use of the PTA system in future RNAi control of lepidopteran pests.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Entomology
Yunhe Fan, Mureed Abbas, Xiaojian Liu, Yanli Wang, Huifang Song, Tao Li, Enbo Ma, Kun Yan Zhu, Jianzhen Zhang
Summary: RNA interference (RNAi) holds great promise for agricultural pest management, but its efficiency varies among different insect species. In this study, six core RNAi pathway genes were identified and characterized from the transcriptome database of the Asian corn borer (Ostrinia furnacalis). The expression of two of these genes, OfDicer2 and OfAgo2, was significantly up-regulated in response to dsEGFP exposure, leading to improved RNAi efficiency against target genes in certain tissues of O. furnacalis. These findings suggest that inducing the expression of key RNAi pathway genes can enhance RNAi efficiency in insects.
Article
Chemistry, Multidisciplinary
Eleonora Maurizi, Davide Alessandro Martella, Davide Schiroli, Alessia Merra, Salman Ahmad Mustfa, Graziella Pellegrini, Claudio Macaluso, Ciro Chiappini
Summary: Nanoneedles can efficiently deliver p16-targeting siRNA to human corneal endothelial cells, inducing p16 silencing and promoting cell proliferation. This approach shows potential for targeted RNA interference therapy in treating endothelial corneal dysfunction.
Review
Chemistry, Medicinal
Song Zhang, Zhujun Cheng, Yanan Wang, Tianyu Han
Summary: The progress of miRNA drugs in clinical trials is slow due to the characteristic of targeting multiple genes, leading to inevitable adverse effects.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Gastroenterology & Hepatology
Sinead M. Smith, Michael Freeley, Paul N. Moynagh, Dermot P. Kelleher
Article
Medicine, Research & Experimental
Sinead T. Aherne, Paul Smyth, Michael Freeley, Leila Smith, Cathy Spillane, John O'leary, Orla Sheils
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2016)
Article
Immunology
Padraic J. Dunne, Christina O. Maher, Michael Freeley, Katie Dunne, Andreea Petrasca, Judy Orikiiriza, Margaret R. Dunne, Derval Reidy, Siobhan O'Dea, Aisling Loy, Jim Woo, Aideen Long, Thomas R. Rogers, Fiona Mulcahy, Derek G. Doherty
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Aideen Long, Michael Freeley
BIOCHEMICAL SOCIETY TRANSACTIONS
(2014)
Review
Cell Biology
Michael Freeley, Dermot Kelleher, Aideen Long
CELLULAR SIGNALLING
(2011)
Article
Immunology
Greg Byrne, Michael Freeley, Con Feighery, Alex Whelan, Aideen Long
CLINICAL IMMUNOLOGY
(2013)
Article
Gastroenterology & Hepatology
Danijela Petrovic, Zania Stamataki, Eugene Dempsey, Lucy Golden-Mason, Michael Freeley, Derek Doherty, David Prichard, Catherine Keogh, Jennifer Conroy, Siobhan Mitchell, Yuri Volkov, Jane A. McKeating, Cliona O'Farrelly, Dermot Kelleher, Aideen Long
Article
Biochemistry & Molecular Biology
Seow Theng Ong, Michael Freeley, Joanna Skubis-Zegadlo, Mobashar Hussain Urf Turabe Fazil, Dermot Kelleher, Friedrich Fresser, Gottfried Baier, Navin Kumar Verma, Aideen Long
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemical Research Methods
Michael Freeley, Aideen Long
JOURNAL OF IMMUNOLOGICAL METHODS
(2013)
Article
Immunology
Michael Freeley, Francis O'Dowd, Toby Paul, Dmitry Kashanin, Anthony Davies, Dermot Kelleher, Aideen Long
JOURNAL OF IMMUNOLOGY
(2012)
Editorial Material
Immunology
Michael Freeley, Aideen Long
FRONTIERS IN IMMUNOLOGY
(2012)
Editorial Material
Pathology
Michael Freeley
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2020)
Article
Medicine, Research & Experimental
Jon P. Day, Ellanor Whiteley, Michael Freeley, Aideen Long, Beatrice Malacrida, Patrick Kiely, George S. Baillie
Article
Biochemical Research Methods
Michael Freeley, Emily Derrick, Eugene Dempsey, Antje Hoff, Anthony Davies, Devin Leake, Annaleen Vermeulen, Dermot Kelleher, Aideen Long
JOURNAL OF BIOMOLECULAR SCREENING
(2015)