Article
Multidisciplinary Sciences
Liliana Felicia Iannucci, Anna Maria D'Erchia, Ernesto Picardi, Daniela Bettio, Filippo Conca, Nicoletta Concetta Surdo, Giulietta Di Benedetto, Deborah Musso, Cristina Arrigoni, Marco Lolicato, Mauro Vismara, Francesca Grisan, Leonardo Salviati, Luciano Milanesi, Graziano Pesole, Konstantinos Lefkimmiatis
Summary: The study reveals an unexpected mechanism by which EPAC1 enters the nucleus and regulates the transcription of a histone cluster by forming biomolecular condensates in its proximity in response to cAMP.
NATURE COMMUNICATIONS
(2023)
Review
Cell Biology
Marion Laudette, Karina Formoso, Frank Lezoualc'h
Summary: This article summarizes the pathophysiological roles of GRK2, GRK5, and Epac1 in the heart, focusing on how they interact under pathological conditions and contribute to the formation of scaffolded signaling systems.
Article
Otorhinolaryngology
Wang Chuan, Li Yuan, Jiang Wen, Zeng Jianwei, Wang Caiji, Zhao Zeqi, Li Yalan, Ji Renlong, Li Kang, Li Wei, Liu Houguang, Liu Wen, Qiao Yuehua, Li Xuanyi
Summary: DDAVP upregulated Epac1 protein expression in the guinea pig cochlea, leading to activation of the inner ear cAMP-Epac1 signaling pathway. This may be an important mechanism by which DDAVP regulates endolymphatic metabolism to induce EH and affect inner ear function.
BRAZILIAN JOURNAL OF OTORHINOLARYNGOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Li Liu, Youde Jiang, Jena Steinle
Summary: The study demonstrates that Epac1 can reduce TLR4 signaling in the retina and in retinal endothelial cells, while direct inhibition of TLR4 can protect the retina against diabetes-induced changes in permeability and cell numbers.
Article
Materials Science, Multidisciplinary
Xiao Gong, Wenjing Li, Yongzhong Wu, Xiaoyan Li, Yan Ma, Hangxiang Wang, Min Wu
Summary: This work proposes a rational strategy for the synthesis of yellow fluorescent carbon nanodots (Y-CDs)-based microparticles with high solid-state photoluminescence. By adjusting the amount of bridge molecule KH-560, the morphology and fluorescence intensity of the microparticles can be controlled. Moreover, the optimal Y-CDs particles can be combined with blue chips to fabricate high-performance white light-emitting diodes with a high color rendering index.
MATERIALS TODAY ADVANCES
(2023)
Article
Optics
Qi Zhu, Qingduo Duanmu
Summary: In this study, the iridium (III)bis[4-(tert-butyl)-2,6-diuoro-2,3-bipyridine]acetylacetonate (FK306) doped layer was utilized as an electron-transport layer to optimize the electron density and improve the electroluminescent performance of yellow TADF devices. With a suitable device design strategy, significant improvements in efficiency were achieved while reducing efficiency roll-off.
Article
Endocrinology & Metabolism
Atsushi Tsuzuki, Yoichiro Fujioka, Aiko Yoshida, Sayaka Kashiwagi, Maho Amano, Tohru Hira, Akinobu Nakamura, Hideaki Miyoshi, Tatsuya Atsumi, Yusuke Ohba
Summary: FP-tagged GLP-1 allows visualization of the secretion process of GLP-1 and displays high efficiency in exocytosis and colocalization with secretory vesicle markers. This tagging method facilitates the study of GLP-1 secretion mechanisms and the discovery of new antidiabetic drugs.
JOURNAL OF DIABETES INVESTIGATION
(2022)
Review
Chemistry, Multidisciplinary
Sohum Mehta, Jin Zhang
Summary: The article discusses the use of biosensors to map the spatial compartmentation of intracellular signaling pathways and illuminate key mechanisms that establish the boundaries of an intricate biochemical activity architecture. It reveals the role of regulatory fences, intracellular membranes in creating unique signaling platforms, and how molecular assemblies enable the precise spatial tuning of biochemical activity.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Review
Chemistry, Analytical
Namdoo Kim, Seunghan Shin, Se Won Bae
Summary: cAMP serves a crucial role in signal transduction pathways, with research focusing on its dynamics leading to insights for drug development and disease treatment. To enable real-time non-invasive imaging, genetically-encoded sensors based on fluorescent proteins and luciferases have been developed as powerful tools.
Article
Pharmacology & Pharmacy
Hongliu Chen, Ying Shen, Yi Liang, Ying Qiu, Meili Xu, Chaoqian Li
Summary: This study investigated the effects and potential mechanism of Selexipag on a mouse model of acute respiratory distress syndrome (ARDS) induced by lipopolysaccharide (LPS) challenge. The results showed that Selexipag could effectively alleviate lung damage in LPS-challenged mice by suppressing inflammation and reducing vascular endothelial injury. The main regulatory molecular mechanism appears to involve lung cAMP and its downstream signaling modules, PKA and Epac1.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Veronica A. Cochrane, Zhongying Yang, Mark L. Dell'Acqua, Show-Ling Shyng
Summary: The study revealed that leptin regulates the trafficking of K-ATP channels in pancreatic beta-cells by affecting PKA activity. Increased PKA activity depends on NMDA receptors, CaMKK beta, and AMPK, and is anchored to AKAP79/150 at the cell membrane. disrupting AKAP79/150 coordination may provide a potential target for restoring insulin secretion in beta-cells with defective leptin signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Zhenjie Sun, Wenhui Gu, Zezhong Feng, Yaqin Fan, Jianfeng Niu, Guangce Wang
Summary: This study elucidated the expression regulation mechanism of the heat shock protein 70 gene in Neopyropia yezoensis under high temperature stress. The findings showed the involvement of cAMP-dependent protein kinase and activating transcription factors element in the transcription regulation of the gene. In addition, AMPK was also found to play a role in the induction of the gene. Furthermore, posttranscriptional mechanisms were proposed to be involved in the synthesis regulation of the heat shock protein 70-2 protein.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Cell Biology
Armando Jesus Perez-Diaz, Beatriz Vazquez-Marin, Jero Vicente-Soler, Francisco Prieto-Ruiz, Teresa Soto, Alejandro Franco, Jose Cansado, Marisa Madrid
Summary: The autophagy pathway in yeast is regulated differently in response to glucose limitation, indicating evolutionary variations in the signaling pathways that govern autophagy during glucose shortage or starvation.
Review
Cell Biology
Alba Guijarro-Belmar, Dominik Mateusz Domanski, Xuenong Bo, Derryck Shewan, Wenlong Huang
Summary: Traumatic spinal cord injury is a global health issue that results in permanent sensorimotor disabilities. While targeting cAMP directly has challenges, activating its downstream effector Epac2 shows promise in promoting axonal regeneration and functional improvement following injury. Further research is needed to fully understand the therapeutic potential of targeting Epac2 in traumatic spinal cord injury.
NEURAL REGENERATION RESEARCH
(2021)
Article
Plant Sciences
Chunqing Wang, Qi Zhang, Jiaxin Li, Xinliang Wang, Chuxin Li, Yu Bin, Zhen Song
Summary: This study reveals that the coat protein of Citrus yellow vein clearing virus (CYVCV) interacts directly with the ascorbate peroxidase 1 of lemon (ClAPX1). The findings suggest that the virus can regulate the antiviral immune response of lemon by manipulating the activity of ClAPX1, possibly through the jasmonic acid signaling pathway.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Ferdinandus, Madoka Suzuki, Cong Quang Vu, Yoshie Harada, Satya Ranjan Sarker, Shin'ichi Ishiwata, Tetsuya Kitaguchi, Satoshi Arai
Summary: Thermal engineering at the microscale presents a significant challenge for basic biological research and biomaterials development. Researchers have developed a nanoparticle that can generate heat spots within live cells and measure the temperature increment using fluorescence thermometry. The results demonstrate the effectiveness of a single heat spot in altering cellular functions.
Article
Biochemistry & Molecular Biology
Bo Zhu, Cheng Qian, Haoxuan Tang, Tetsuya Kitaguchi, Hiroshi Ueda
Summary: In this study, a thermostable GUS enzyme switch based on the mutant DLW was developed by disrupting a conserved salt bridge, which showed up to 1.8-fold signal-to-background ratio at high temperatures. Molecular dynamics simulations were used to predict the structural changes, highlighting DLW as a versatile tool for developing thermostable immunosensors.
Article
Endocrinology & Metabolism
Fumiya Uefune, Toru Aonishi, Tetsuya Kitaguchi, Harumi Takahashi, Susumu Seino, Daisuke Sakano, Shoen Kume
Summary: There is growing evidence that dopamine functions as a negative regulator of insulin secretion, but the underlying molecular mechanism remains unknown. This study found that D1 receptors play a role in the DA-mediated inhibition of insulin secretion, while overexpression of D2 receptors has a toxic effect on beta-cells. D1 and D2 receptors form heteromers in beta-cells, and D1 protects beta-cells from the harmful effects of DA by modulating D2 signaling.
Article
Biology
Mai Takizawa, Yuri Osuga, Rika Ishida, Marie Mita, Kazuki Harada, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi
Summary: A red fluorescent protein-based indicator, Red cGull, allows for multi-colour imaging and optogenetic applications while monitoring cGMP activity in cells.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biophysics
Jiulong Su, Bo Zhu, Akihito Inoue, Hiroyuki Oyama, Izumi Morita, Jinhua Dong, Takanobu Yasuda, Yoshiko Sugita-Konishi, Tetsuya Kitaguchi, Norihiro Kobayashi, Shiro Miyake, Hiroshi Ueda
Summary: Patrol Yeast is a novel yeast-based immunosensor that can detect toxic substances in food. A membrane-based yeast two-hybrid system using split-ubiquitin was used to successfully detect important concentrations of caffeine and aflatoxins. Additionally, it can detect enterohemorrhagic Escherichia coli.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Kazuki Harada, Maoko Takashima, Tetsuya Kitaguchi, Takashi Tsuboi
Summary: We investigated the dynamics of GLP-1 exocytosis and found that it displayed a biphasic pattern, with most exocytosis occurring from pre-docked granules or rapidly fused granules without stable docking. Granules that docked with the plasma membrane after stimulation and subsequently fused were predominant. Inhibition of actin polymerization suppressed exocytosis of pre-docked granules. These results suggest that the docking dynamics of GLP-1 granules show a time-dependent biphasic shift, which is determined by interaction with F-actin.
Article
Chemistry, Multidisciplinary
Yoshihiro Ito, Ryuichi Sasaki, Sayaka Asari, Takanobu Yasuda, Hiroshi Ueda, Tetsuya Kitaguchi
Summary: In this study, a simple and versatile high-throughput screening method for protein-secreting bacterial strains is developed using microemulsions. The secreted protein is detected using a fluorescent immunosensor and selected using a cell sorter. This method was successfully used to screen and improve the secretion ability of Corynebacterium glutamicum strains, increasing the secretion of fibroblast growth factor 9 threefold compared to the parent strain. This screening method can be applied to a wide range of proteins by fusing a small detection tag, and it shortens the development period of industrial strains for biopharmaceutical protein production.
Article
Cell Biology
Donnell White, Lothar Lauterboeck, Parnia Mobasheran, Tetsuya Kitaguchi, Antoine H. Chaanine, Qinglin Yang
Summary: A new fluorescent ATP indicator called smacATPi has been developed, allowing real-time visualization of ATP levels and dynamics in both the cytosol and mitochondria. This concept provides valuable insights into cellular metabolism and its response to metabolic changes.
Article
Chemistry, Analytical
Bo Zhu, Zhe Du, Yancen Dai, Tetsuya Kitaguchi, Sebastian Behrens, Burckhard Seelig
Summary: In vitro compartmentalization (IVC) is a technique used to establish the genotype-phenotype linkage, and fluorinated oils have become more widely used in making microdroplets. However, performing multi-step reactions in water-in-fluorinated-oil microdroplets is difficult. To overcome this issue, a nanodroplet-based approach for the delivery of copper ions and middle-sized peptide molecules has been demonstrated.
Article
Chemistry, Multidisciplinary
Sohei Hiasa, Takeru Fujimori, Saki Aiki, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi
Summary: Researchers have developed a green fluorescent cAMP indicator called "Green Falcans" that can visualize cAMP dynamics at low concentrations and observe the kinetics of cAMP in various pathways with high spatiotemporal resolution. This study demonstrates the potential of multi-color imaging in understanding hierarchical and cooperative interactions with other molecules in cAMP signaling pathways.
Review
Chemistry, Analytical
Abdul Qawee Rani, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi
Summary: Homogeneous immunosensors combine the advantages of biosensors and immunoassays, offering speed, high sensitivity, and accuracy. They have seen rapid development in recent years and provide a cost-effective alternative technology with rapidity, sensitivity, and user-friendliness, which has been widely applied. This review introduces current trends in immunosensor development, particularly fluorescent and bioluminescent immunosensors, highlighting their advantages, improvements, and key approaches to address limitations.
Article
Biochemistry & Molecular Biology
Saki Tsuno, Kazuki Harada, Mina Horikoshi, Marie Mita, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi
Summary: This study visualized the dynamics of metabolites in hepatocytes during recovery from glucose-deprivation. The results suggest that a decrease in mitochondrial ATP concentration is associated with increased protein synthesis rather than glycogen synthesis, activation of urea cycle, and production of reactive oxygen species.
Article
Chemistry, Analytical
Bo Zhu, Nobuyuki Nosaka, Shuji Kanamaru, Jinhua Dong, Yancen Dai, Akihito Inoue, Yinghui Yang, Kaori Kobayashi, Tetsuya Kitaguchi, Hiroshi Iwasaki, Ryuji Koike, Kenji Wakabayashi, Hiroshi Ueda
Summary: This study developed a homogeneous immunosensor based on a human anti-N protein antibody for SARS-CoV-2. The addition of a crowding agent significantly improved the response speed and sensitivity of the immunosensor. The developed immunosensor was validated in testing specimens from COVID-19-positive patients.
Article
Chemistry, Multidisciplinary
Yancen Dai, Yuko Sato, Bo Zhu, Tetsuya Kitaguchi, Hiroshi Kimura, Farid J. Ghadessy, Hiroshi Ueda
Summary: This study developed a conditionally active Q-body probe for precise imaging of intracellular p53 in living cells with low background signal. The Q-body showed significant fluorescence enhancement in vitro and in both fixed and live cells. Live-cell sorting based on p53 expression was also demonstrated.
Article
Biochemistry & Molecular Biology
Marie Mita, Izumi Sugawara, Kazuki Harada, Motoki Ito, Mai Takizawa, Kentaro Ishida, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi
Summary: In this study, a new glucose indicator called Red Glifons was developed for real-time monitoring of intracellular glucose dynamics. These indicators exhibited high sensitivity and a wide range of applications, making them valuable tools for studying complex interactions in energy metabolism.
CELL CHEMICAL BIOLOGY
(2022)
