Article
Biochemical Research Methods
Ryuichi Hirota, Zen-ichiro Katsuura, Naoki Momokawa, Hiroki Murakami, Satoru Watanabe, Takenori Ishida, Takeshi Ikeda, Hisakage Funabashi, Akio Kuroda
Summary: In this study, it was found that under specific conditions, a phosphite-dependent strain of Escherichia coli can generate escape mutants. The mutation occurs at position 210 of the transmembrane domain protein HtxC of the phosphorus compound transporter HtxBCDE. The replacement of a specific amino acid residue at this position can reduce the rate of escape mutant generation.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yaolei Mi, Xue Cao, Xuewen Zhu, Weiqiang Chen, Xiangxiao Meng, Huihua Wan, Wei Sun, Sifan Wang, Shilin Chen
Summary: This study identified and characterized 113 ABC transporters in C. sativa, and proposed seven core transporters that may participate in cannabinoid transport. These candidate genes showed high correlation with cannabinoid biosynthetic pathway genes and content, and were highly expressed in cannabinoid biosynthesis and accumulation sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Qingshan Luo, Huigang Shi, Xueqing Xu
Summary: Lipopolysaccharide (LPS) is a crucial component of the outer membrane in most Gram-negative bacteria, and its transport from the inner membrane to the outer membrane is facilitated by seven lipopolysaccharide transport proteins. This study presents the cryo-EM structures of LptB(2)FG and LptB(2)FGC from Klebsiella pneumoniae, highlighting the detailed interactions between LPS and the transporter.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Microbiology
Aissatou Maty Diagne, Anais Pelletier, Claire Durmort, Agathe Faure, Kerstin Kanonenberg, Celine Freton, Adeline Page, Frederic Delolme, Jaroslav Vorac, Sylvain Vallet, Laure Bellard, Corinne Vives, Franck Fieschi, Thierry Vernet, Patricia Rousselle, Sebastien Guiral, Christophe Grangeasse, Jean-Michel Jault, Cedric Orelle
Summary: This study demonstrates that TCS01 cooperates with the BceAB-type ABC transporter to sense and induce resistance to bacterial-derived antimicrobial peptides, ultimately enhancing the survival of Streptococcus pneumoniae in the human host.
Article
Pharmacology & Pharmacy
Gangyang Wang, Lingling Cao, Yafei Jiang, Tao Zhang, Hongsheng Wang, Zhuoying Wang, Jing Xu, Min Mao, Yingqi Hua, Zhengdong Cai, Xiaojun Ma, Shuo Hu, Chenghao Zhou
Summary: This study demonstrates that anlotinib can reverse multidrug resistance in osteosarcoma cells by inhibiting the efflux function of P-glycoprotein 1 (PGP1) and increasing the intracellular accumulation of chemotherapeutic agents. Anlotinib also stimulates the ATPase activity of PGP1. In animal studies, anlotinib combined with doxorubicin shows a significant decrease in tumor growth rate and tumor size.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Methinee Pipatthana, Phurt Harnvoravongchai, Pisut Pongchaikul, Somsak Likhitrattanapisal, Matthew Phanchana, Surang Chankhamhaengdecha, Tavan Janvilisri
Summary: This study analyzed the sequence-function relationship of ABC proteins in Clostridioides difficile, revealing different protein domains associated with the ABC system and the gene organization within the genome. The classification and genetic structure of ABC transporters were identified, with a focus on the significant role of these proteins in antibiotic resistance, providing insights into the function and evolutionary relationships of ABC proteins in this pathogen.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Cedric Orelle, Lutz Schmitt, Jean-Michel Jault
Summary: Microorganisms rely on ATP-binding cassette (ABC) transporters, which are membrane proteins, to exchange with their environment for nutrients and remove toxic compounds. While these transporters play a protective role in organisms by expelling unrelated compounds, they can also contribute to resistance to therapeutic treatments. Interestingly, some multidrug ABC transporters exhibit high levels of ATPase activity even without substrates. This inherent activity allows them to efficiently transport drugs before they penetrate into the cell.
TRENDS IN MICROBIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Mohammad Feyzizadeh, Ashkan Barfar, Zeinab Nouri, Muhammad Sarfraz, Parvin Zakeri-Milani, Hadi Valizadeh
Summary: This review discusses various strategies to overcome multidrug resistance (MDR) in cancer therapy, including conventional synthetic and natural inhibitors, as well as novel approaches such as RNA, monoclonal antibodies, nanobiotechnology, and structural modification techniques. The experts emphasize the importance of understanding the structure of ABC transporters to design rational inhibitors and highlight the potential of herbal products in reversing MDR.
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Stephanie L. Neville, Jennie Sjoehamn, Jacinta A. Watts, Hugo MacDermott-Opeskin, Stephen J. Fairweather, Katherine Ganio, Alex Carey Hulyer, Aaron P. McGrath, Andrew J. Hayes, Tess R. Malcolm, Mark R. Davies, Norimichi Nomura, So Iwata, Megan L. O'Mara, Megan J. Maher, Christopher A. McDevitt
Summary: Metal ions are essential for all forms of life. ATP-binding cassette permeases play a key role in importing micronutrients, like manganese, in prokaryotes. This study has identified important structural features, such as extracellular gating residues and metal coordination sites, that are critical for manganese transport.
Article
Multidisciplinary Sciences
Min Woo Sung, Zhongying Yang, Camden M. Driggers, Bruce L. Patton, Barmak Mostofian, John D. Russo, Daniel M. Zuckerman, Show-Ling Shyng
Summary: The structure and activation mechanism of vascular K-ATP channels differ from other K-ATP channel isoforms, showing unique properties. Experimental and simulation studies have revealed key interactions between subunits related to activity regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Silpa Narayanan, Ying-Fang Fan, Nehaben A. Gujarati, Qiu-Xu Teng, Jing-Quan Wang, Chao-Yun Cai, Yuqi Yang, Anirudh J. Chintalapati, Yixiong Lei, Vijaya L. Korlipara, Zhe-Sheng Chen
Summary: VKNG-1 selectively inhibits ABCG2 transporter and reverses resistance to standard anticancer drugs, providing a potential treatment for ABCG2-mediated MDR in colon cancer.
Article
Multidisciplinary Sciences
Andrzej Harris, Manuel Wagner, Dijun Du, Stefanie Raschka, Lea-Marie Nentwig, Holger Gohlke, Sander H. J. Smits, Ben F. Luisi, Lutz Schmitt
Summary: Pdr5, a member of the ABC transporter superfamily, plays a crucial role in drug efflux through an ATP-driven conformational cycle. The structural analysis reveals that while half of the transporter remains unchanged throughout the cycle, its partner undergoes changes to support a peristaltic motion for pumping molecules.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Environmental
Haibei Li, Xinmei Li, Tianjiao Chen, Zhongwei Yang, Danyang Shi, Jing Yin, Dong Yang, Shuqing Zhou, Junwen Li, Min Jin
Summary: This study found that certain antidepressants can induce bacterial resistance to disinfectants and activate certain genes to increase bacterial efflux of disinfectants, thereby increasing resistance. This provides novel insights into the potential source of water-borne disinfectant-resistant pathogens.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Medicine, Research & Experimental
Zhuo-Xun Wu, Qiuyan Mai, Yuqi Yang, Jing-Quan Wang, Hansu Ma, Leli Zeng, Zhe-Sheng Chen, Yihang Pan
Summary: Overexpression of ABCG2 transporter leads to resistance of cancer cells to GSK1070916, and combination with an ABCG2 inhibitor can restore the sensitivity of GSK1070916.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Plant Sciences
Joanna Banasiak, Tomasz Jamruszka, Jeremy D. Murray, Michal Jasinski
Summary: Most land plants establish symbiosis with beneficial soil microbes, providing nutrition through endosymbiotic processes. Host transport systems play a crucial role in these symbioses by modulating the expression and function of plant transporter systems.