Article
Biochemistry & Molecular Biology
Fei Jin, Minxuan Sun, Takashi Fujii, Yurika Yamada, Jin Wang, Andres D. Maturana, Miki Wada, Shichen Su, Jinbiao Ma, Hironori Takeda, Tsukasa Kusakizako, Atsuhiro Tomita, Yoshiko Nakada-Nakura, Kehong Liu, Tomoko Uemura, Yayoi Nomura, Norimichi Nomura, Koichi Ito, Osamu Nureki, Keiichi Namba, So Iwata, Ye Yu, Motoyuki Hattori
Summary: Our study revealed the transmembrane structure of MgtE in the absence of Mg2+ ions through cryo-electron microscopy, showing the Mg2+-dependent pore opening mechanism and the kink motions of TM2 and TM5 helices at glycine residues crucial for channel activity. This provides structure-based mechanistic insights into the channel gating of MgtE.
Review
Biochemistry & Molecular Biology
Mathias Ziegler, Magnus Monne, Andrey Nikiforov, Gennaro Agrimi, Ines Heiland, Ferdinando Palmieri
Summary: Subcellular compartmentation is a fundamental property of eukaryotic cells, where the transport of solutes across membranes plays a crucial role in cellular communication and metabolic regulation. In mammals, the SLC family comprises carriers that selectively transport molecules across biological membranes, with the SLC25 family playing a particularly important role in supplying mitochondria with metabolic intermediates. The recent discovery of SLC25A51 as the major mitochondrial NAD(+) carrier in mammals has resolved a long-standing mystery in NAD(+) biology.
Article
Biochemistry & Molecular Biology
Jiaheng Xie, Zhechen Zhu, Yuan Cao, Shujie Ruan, Ming Wang, Jingping Shi
Summary: SLC16A1 is a robust prognostic biomarker for melanoma, associated with decreased overall survival in patients. It plays important roles in metabolic regulation, protein ubiquitination, and substance localization, and may serve as a novel target in immunotherapy.
Article
Multidisciplinary Sciences
Maria F. Noterman, Kalyani Chaubey, Kristi Lin-Rahardja, Anjali M. Rajadhyaksha, Andrew A. Pieper, Eric B. Taylor
Summary: Researching the composition and function of brain mitochondria is challenging but crucial for developing improved neuroprotective therapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Kelly Urbanek, Danica M. Sutherland, Robert C. Orchard, Craig B. Wilen, Jonathan J. Knowlton, Pavithra Aravamudhan, Gwen M. Taylor, Herbert W. Virgin, Terence S. Dermody
Summary: Through a CRISPR cell survival screen, two genes - Cmas and Slc35a1 were identified as host genes required for reovirus infection of microglial cells by promoting sialic acid expression on the cell surface. These findings expand understanding of receptors engaged by reovirus on microglial cells and may lead to new strategies for targeting microglial cells for oncolytic applications.
JOURNAL OF VIROLOGY
(2021)
Article
Pharmacology & Pharmacy
Ole Jensen, Muhammad Rafehi, Lukas Gebauer, Jurgen Brockmoeller
Summary: Psychostimulants and hallucinogens have been found to be substrates of OCTs, with genetic polymorphisms in OCT1 playing a significant role in the cellular uptake of mescaline.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Endocrinology & Metabolism
Jiamei Le, Yi Fu, Qiuqin Han, Xindong Wei, Houlin Ji, Yifan Chen, Qiuying Wang, Peixian Pi, Jilei Li, Xinjie Lin, Xiaoying Zhang, Yong Zhang, Jianping Ye
Summary: Metformin improves insulin sensitivity in type 2 diabetes by targeting liver, intestine, and other organs. This study found that more genes were altered in the liver than in the colon of diet-induced obese mice, with 20 Slc genes confirmed to be altered in the liver and 15 of them restored by MET, leading to improved insulin sensitivity and obesity.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Eugene C. Chen, Par Matsson, Mina Azimi, Xujia Zhou, Niklas Handin, Sook Wah Yee, Per Artursson, Kathleen M. Giacomini
Summary: This study identified several prescription drugs as inhibitors of OCT3, with some compounds showing potent inhibition at therapeutic unbound plasma levels, which may lead to drug-drug interactions or off-target pharmacologic effects.
PHARMACEUTICAL RESEARCH
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Solene Marie, Irene Hernandez-Lozano, Oliver Langer, Nicolas Tournier
Summary: Hepatocyte transporters play a crucial role in the hepatobiliary elimination of various substances, influenced by different pathophysiological conditions and specific drugs; Tc-99m-mebrofenin has been used for hepatobiliary scintigraphy for years, but the specific transporters controlling its liver kinetics have only recently been characterized; Pharmacokinetic modeling offers a translational method to study transporter-mediated drug interactions, providing insights into liver function as a biomarker.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Genetics & Heredity
Wei Wei, Rubin Xu, Xiaomei Ying, Liang Chen, Xiaohuan Lu, Qikai Tang, Jiaheng Xie, Hongzhu Yu
Summary: This study constructed a prognostic signature consisting of nine SLC genes in hepatocellular carcinoma (HCC) through comprehensive analysis. By calculating risk scores, HCC patients could be divided into high-risk and low-risk groups, and a hub gene SLC7A11 was identified as a robust prognostic marker of HCC.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Yue Li, Weidong Wang, Hui-Ying Lim
Summary: Through genetic studies, it was found that the regulation of glucose absorption in the Drosophila midgut is mediated by the Solute Carrier 5A5 (dSLC5A5). Genetically knocking down dSLC5A5 in flies leads to decreased glucose and trehalose levels in the system and circulation, while overexpression of dSLC5A5 increases these levels and promotes glucose uptake in enterocytes. Additionally, it was shown that dSLC5A5 undergoes apical endocytosis in a dynamin-dependent manner, crucial for glucose uptake in enterocytes, and that glucose upregulates dSLC5A5 levels in the midgut, influencing systemic glucose homeostasis on a high-sugar diet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Charles R. Lefevre, Marc Le Vee, Sophie Gaubert, Elodie Jouan, Arnaud Bruyere, Caroline Moreau, Olivier Fardel
Summary: The study analyzed the characteristics of 15 OCT2 substrates and found that only 4 of them exhibited trans-stimulation effect, with differences in molecular properties compared to non-trans-stimulating substrates. Therefore, the sensitivity of the trans-stimulation assay for identifying OCT2 substrates was relatively low, caution should be exercised in the use of this method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Rachel-Ann A. Garibsingh, Elias Ndaru, Alisa A. Garaeva, Yueyue Shi, Laura Zielewicz, Paul Zakrepine, Massimiliano Bonomi, Dirk J. Slotboom, Cristina Paulino, Christof Grewer, Avner Schlessinger
Summary: ASCT2 is up-regulated in cancer and plays a crucial role in promoting cell proliferation by modulating intracellular glutamine levels; Inhibiting nutrient uptake via ASCT2 could be a potential strategy for cancer therapy; We designed a series of unique ASCT2 inhibitors using computational modeling and cryo-EM, providing a framework for the development of cancer therapeutics targeting ASCT2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Tania Love Aaes, Javier Burgoa Cardas, Kodi S. Ravichandran
Summary: In this study, RNA-Seq data sets were analyzed to explore the transcriptome of immune cells, specifically focusing on the expression of Solute carrier (SLC) transporters. Differential expression of SLC transcripts between phagocytes and adaptive immune cells was identified, and new potential immune cell markers based on SLC expression were discovered. User-friendly online tools were also provided for researchers to compare SLC genes and other genes among immune cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaoli Zhang, Yiming Ma, Jiafu Ji, Xinhua Zhao, Junhu Yuan, Hongying Wang, Guoqing Lv
Summary: This study aimed to investigate whether a high-fat diet (HFD) affects ferroptosis and colitis progression in an obesity-independent manner. The results showed that non-obesogenic HFD reduced colonic mucosal injury and decreased lipid peroxidation and ferroptosis markers expression in colon tissues.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)