Article
Biophysics
Naba Mukhtar, Eric N. Cytrynbaum, Leah Edelstein-Keshet
Summary: Epithelial-mesenchymal transition (EMT) plays a crucial role in wound healing, embryonic development, and cancer metastasis. This study presents a multiscale model that investigates the role of the protein YAP in promoting EMT and validates the simulation results with experimental observations.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Sucharat Tungsukruthai, Boonchoo Sritularak, Pithi Chanvorachote
Summary: Lung cancer metastasis is a major cause of lung cancer-related deaths. This study investigated the potential of cycloartocarpin in inhibiting the metastasis of lung cancer cells. The results showed that non-toxic concentrations of cycloartocarpin effectively suppressed cell migration and attenuated anchorage-independent cell growth. This was accompanied by the inhibition of the EMT process through the suppression of the FAK/AKT signaling pathway.
Article
Biochemistry & Molecular Biology
Tsugumasa Toma, Hiroshi Tateishi, Kensaku Kawakami, Taha F. S. Ali, Masahiro Kamo, Kazuaki Monde, Yuta Nakashima, Mikako Fujita, Masami Otsuka
Summary: Metastasis is the main cause of mortality in solid tumors, but there are no available antimetastatic drugs on the market. This study focused on a synthetic compound called HPH-15, which showed anti-EMT and anti-cell migration activities in non-small-cell lung cancer cells. HPH-15 was found to inhibit downstream TGF-beta signaling and could potentially lead to the development of new antimetastatic drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kayla Templeton, Meiby Ramos, Jacqueline Rose, Bryan Le, Qingwen Zhou, Amin Cressman, Stephanie Ferreyra, Carlito B. Lebrilla, Fernando Antonio Fierro
Summary: The study demonstrates that sialylation of N-glycans in MSCs is tightly regulated in response to environmental cues, with the inhibition of sialylation by 3F-Neu5Ac resulting in increased adhesion, migration, and survival of MSCs, while also inhibiting osteogenic and adipogenic differentiation. Additionally, the secretion of key trophic factors by MSCs is variably affected upon exposure to 3F-Neu5Ac, suggesting glycoengineering MSCs to reduce sialylated N-glycans could enhance their therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Ning Zhao, Minwei He, Wei Chen, Peng Jin, Lulu Cao, Jinhai Deng, Xu Cheng, Lu Wang
Summary: Our study identified FAM96A as a crucial regulator of tumor metastasis and epithelial-mesenchymal transition (EMT) by inhibiting the TGF-beta signaling pathway.
Article
Biochemistry & Molecular Biology
Iveta Yotova, Quanah J. Hudson, Florian M. Pauler, Katharina Proestling, Isabella Haslinger, Lorenz Kuessel, Alexandra Perricos, Heinrich Husslein, Rene Wenzl
Summary: LINC01133 is upregulated in endometriosis and may affect pathogenesis through cellular proliferation and migration pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ali Dayoub, Artem I. Fokin, Maria E. Lomakina, John James, Marina Plays, Tom Jacquin, Nikita M. Novikov, Rostislav S. Vorobyov, Anastasia A. Schegoleva, Karina D. Rysenkova, Julia Gaboriaud, Sergey V. Leonov, Evgeny V. Denisov, Alexis M. Gautreau, Antonina Y. Alexandrova
Summary: Whole exome sequencing of invasive mammary carcinomas identified the association of PTEN and ZFHX3 mutations. In MCF10A cells, PTEN inactivation induced the formation of large colonies, while ZFHX3 inactivation in PTEN KO increased colony numbers and restored their size. PTEN KO enhanced coordinated cell motility and collective migration, while ZFHX3 KO resulted in uncoordinated cell movement and the expression of the mesenchymal marker vimentin. Double KO cells showed decreased coordination in collective migration, high vimentin levels, and restored linear adhesive junctions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bowen Diao, Chongfeng Sun, Panpan Yu, Zouyu Zhao, Ping Yang
Summary: This study investigates the functional influences of LAMA5 knockdown on ovarian cancer (OC) in vitro and in vivo, and finds that LAMA5 is highly connected to OC progression by playing a role in the epithelial-mesenchymal transition (EMT) process through the Notch signaling pathway.
Review
Oncology
Evan M. McCabe, Theodore P. Rasmussen
Summary: The epithelial to mesenchymal transition (EMT) is a cellular process crucial in embryogenesis, where cancer stem cells (CSCs) play a role by differentiating and undergoing EMT. Long noncoding RNAs (lncRNAs) also have a role in CSCs and EMT, with the potential to serve as biomarkers and therapeutic targets in cancer progression.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Young Y. Jung, Arunachalam Chinnathambi, Tahani A. Alahmadi, Sulaiman A. Alharbi, Alan P. Kumar, Gautam Sethi, Kwang S. Ahn
Summary: This study demonstrates that Fangchinoline (FCN) can inhibit the epithelial-mesenchymal transition (EMT) in human colon cancer cells, thereby reducing their metastatic and invasive capacities. In addition, FCN can suppress multiple cell signaling pathways, including c-Met/PI3K/Akt/mTOR and Wnt/β-catenin pathways. Therefore, FCN has the potential to be a novel antimetastatic agent against colon cancer.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Oncology
Feifeng Lan, Menghua Chen, Xiaowei Xie, Yanyan Mo, Fengti Chen, Renbin Huang, Wenqi Liu
Summary: YLSPS, derived from the root of Millettia pulchra var. laxior, exhibits anticancer effects in lung cancer cells by reversing EMT and inhibiting the TGF-beta 1-induced ERK signaling pathway. YLSPS significantly inhibits the growth of xenograft tumors and reduces the levels of proteins associated with EMT. Importantly, YLSPS has fewer toxic side effects than cisplatin.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Tzu-Yin Chen, Chia-Hao Liu, Tsung-Hsien Chen, Mei-Ru Chen, Shan-Wen Liu, Pinpin Lin, Kurt Ming-Chao Lin
Summary: The role of epithelial-mesenchymal transition in lung diseases and cancer related to cigarette smoking is significant. Adipose-derived stem cell-conditioned medium shows protective effects against cigarette smoke-induced cell death and EMT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Young Yun Jung, Chakrabhavi Dhananjaya Mohan, Huiyan Eng, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Kanchugarakoppal S. Rangappa, Gautam Sethi, Alan Prem Kumar, Kwang Seok Ahn
Summary: This study demonstrates that 2,3,5,6-Tetramethylpyrazine (TMP) can suppress the EMT program in colon cancer cells by regulating MnSOD and related proteins, and inhibit the proliferation, migration, and invasion of tumor cells.
Review
Pharmacology & Pharmacy
Sara Lovisa
Summary: EMT, a process crucial in wound healing, fibrosis, and cancer, has gained significant attention from researchers since its discovery in the seventies. Despite advances in understanding its functional role, targeting EMT in fibrotic diseases remains a challenge at an early stage of development.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kittipong Sanookpan, Nongyao Nonpanya, Boonchoo Sritularak, Pithi Chanvorachote
Summary: The study demonstrated that ovalitenone suppresses EMT via inhibition of the AKT/mTOR signaling pathway and has potential for inhibiting CSC phenotypes. These findings reveal the anti-metastasis potential of the compound and support the development of ovalitenone treatment for lung cancer therapy.
Letter
Cell Biology
Li Yang, Jin Chen, Jianqing Liang, Yufeng Zhang, Qingzhe Wang, Xiaojun Ren, Jinsong Wei, Qianchun Gong, Jiting Zhang, Ning Jiang, Xinhua Lin, Jin Li, Bing Zhao
Article
Chemistry, Multidisciplinary
Jianqing Liang, Jun Qian, Li Yang, Xiaojun Chen, Xiaoning Wang, Xinhua Lin, Xiaoyue Wang, Bing Zhao
Summary: This study provides a cell atlas and organoid model for human thyroid development, shedding light on the heterogeneous population of thyrocytes and its regulatory signaling. The activation of cAMP signaling was found to enhance follicle maturation and thyroid hormone secretion. Furthermore, the study reveals the importance of chromatin accessibility for hormone production.
Article
Gastroenterology & Hepatology
Renqiang Sun, Zhiyong Zhang, Ruoxuan Bao, Xiaozhen Guo, Yuan Gu, Wenjing Yang, Jinsong Wei, Xinyu Chen, Lingfeng Tong, Jian Meng, Chen Zhong, Cheng Zhang, Jinye Zhang, Yiping Sun, Chen Ling, Xuemei Tong, Fa-Xing Yu, Hongxiu Yu, Weifeng Qu, Bing Zhao, Wei Guo, Maoxiang Qian, Hexige Saiyin, Ying Liu, Rong-Hua Liu, Cen Xie, Weiren Liu, Yue Xiong, Kun-Liang Guan, Yinghong Shi, Pu Wang, Dan Ye
Summary: This study uncovers a new function of SIRT5 in regulating bile acid metabolism to prevent tumor immune evasion and suppress HCC development.
JOURNAL OF HEPATOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Guofeng Liu, Jinsong Wei, Xiaoyu Li, Meng Tian, Zhenxing Wang, Congcong Shen, Wan Sun, Chonghui Li, Xuewen Li, Enguang Lv, Shizheng Tian, Jihua Wang, Shicai Xu, Bing Zhao
Summary: Exploring advanced techniques for protein detection is important for investigating cell fate. This study reports a luminescent approach using upconversion nanoparticles (UCNPs) to detect the hepatic cell fate marker albumin. An OFF-ON strategy is adopted to ensure detection quality and accuracy. The UCAR nanoprobe can sense albumin in various bio-samples and enable real-time monitoring of hepatocyte differentiation.
Article
Biochemistry & Molecular Biology
Wei Wei, Xiaofang Tang, Ning Jiang, Chao Ni, Hua He, Shenfei Sun, Meng Yu, Chuyue Yu, Mengdi Qiu, Dong Yan, Zhaocai Zhou, Yuanlin Song, Hanmin Liu, Bing Zhao, Xinhua Lin
Summary: This study reveals the crucial role of the chromatin remodeler Znhit1/H2A.Z in regulating lung branching morphogenesis. Znhit1 deficiency leads to failure of branching morphogenesis and neonatal lethality in the embryonic lung epithelium, partially due to the derepression of the BMP4 gene. Inhibition of BMP signaling rescues the lung branching defects in Znhit1 mutants.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Xiaojun Ren, Jianqing Liang, Yiming Zhang, Ning Jiang, Yuhui Xu, Mengdi Qiu, Yiqin Wang, Bing Zhao, Xiaojun Chen
Summary: This study used single-cell transcriptomics to characterize the development of endometrioid endometrial cancers (EEC) and revealed potential cells of origin. The findings provide important insights for EEC research and diagnosis.
NATURE COMMUNICATIONS
(2022)
Review
Oncology
Yin Li, Xinyu Gao, Chao Ni, Bing Zhao, Xinghua Cheng
Summary: Lung cancer is a common and deadly disease, but its progression mechanisms are not well understood and new treatments are continually being developed. Patient-derived organoids (PDOs) are a novel preclinical model that can be established from the patient's tumor tissue and cultured in the lab, preserving the key characteristics of the original tumor. Compared to other models, PDOs have higher success rates, lower establishment time and cost, and offer more individualized prediction of treatment efficacy. This paper summarizes the current application of PDOs in translational research for lung cancer.
Article
Cell & Tissue Engineering
Yuhui Xu, Jiali Hu, Qiaoying Lv, Chenyi Shi, Mengdi Qiu, Liying Xie, Wei Liu, Bingyi Yang, Weiwei Shan, Yali Cheng, Bing Zhao, Xiaojun Chen
Summary: This study aimed to investigate the potential therapeutic effects and underlying mechanisms of mesenchymal stem cells (MSCs) on well-differentiated endometrial cancer (EC). The results demonstrated that endometrium-derived MSCs (eMSCs) had the highest inhibitory effect on EC cell viability and xenograft tumor growth compared to adipose-derived MSCs (AD-MSCs) and umbilical-cord-derived MSCs (UC-MSCs). The study also revealed that eMSCs inhibited EC cell proliferation and stemness through the DKK1-Wnt/beta-catenin signaling pathway. Furthermore, the combination of eMSCs and medroxyprogesterone acetate (MPA) showed enhanced inhibition of EC growth. These findings suggest that eMSCs may be a promising therapeutic strategy for young EC patients desiring fertility preservation.
STEM CELL RESEARCH & THERAPY
(2023)
Editorial Material
Cell & Tissue Engineering
Jinsong Wei, Wen Zhang, Bing Zhao
Summary: A study demonstrated the use of human fetal liver organoids to model steatosis, leading to the discovery of drugs and a key regulator for treating fatty liver disease.
Editorial Material
Cell & Tissue Engineering
Yalong Wang, Hanqing Lin, Lianzheng Zhao, Fan Hong, Jie Hao, Zhen Zhang, Weiqi Sheng, Linhong Song, Chu-Xia Deng, Bing Zhao, Jiani Cao, Lei Wang, Liu Wang, Lingmin Liang, Wenli Kelly Chen, Chunping Yu, Zhijian Sun, Yingying Yang, Changlin Wang, Yong Zhang, Qiyuan Li, Ka Li, Aijin Ma, Tongbiao Zhao, Guoqiang Hua, Ye-Guang Chen
Summary: "Human Intestinal Organoids" is the first guideline on human intestinal organoids in China, providing standards for quality control and promoting international standardization in this field.
Article
Medicine, General & Internal
Zhongguang Luo, Bangting Wang, Feifei Luo, Yumeng Guo, Ning Jiang, Jinsong Wei, Xin Wang, Yujen Tseng, Jian Chen, Bing Zhao, Jie Liu
Summary: This study established a promising HRCA-PDO biobank and conducted the first high-throughput and high-content HRCA drug screening in order to shed light on the prevention of colorectal cancer.
Editorial Material
Cell & Tissue Engineering
Hanqing Lin, Yalong Wang, Chunyan Cheng, Yuxin Qian, Jie Hao, Zhen Zhang, Weiqi Sheng, Linhong Song, Chu-Xia Deng, Bing Zhao, Jiani Cao, Lei Wang, Liu Wang, Lingmin Liang, Wenli Kelly Chen, Chunping Yu, Zhijian Sun, Yingying Yang, Changlin Wang, Yong Zhang, Qiyuan Li, Ka Li, Aijin Ma, Tongbiao Zhao, Ye-Guang Chen, Guoqiang Hua
Summary: Human intestinal cancer organoids have emerged as important in vitro models for colorectal cancer research. The publication of the "Human Intestinal Cancer Organoids" standard in China will guide the production and quality control of these organoids, and promote international standardization.
Article
Biotechnology & Applied Microbiology
Junbo Liang, Jinsong Wei, Jun Cao, Jun Qian, Ran Gao, Xiaoyu Li, Dingding Wang, Yani Gu, Lei Dong, Jia Yu, Bing Zhao, Xiaoyue Wang
Summary: This study develops a framework called OSCAR, which uses regulon activities as readouts to analyze the effects of gene knockout in organoids. By applying this framework in liver organoids, known and novel positive and negative regulators involved in hepatocyte formation are identified. Further experiments confirm the roles of Fos and Ubr5 in promoting hepatocyte differentiation and delaying hepatocyte maturation, respectively.
Article
Engineering, Biomedical
Jin Chen, Jiting Zhang, Li Yang, Bing Zhao
BIO-DESIGN AND MANUFACTURING
(2023)
Review
Cell & Tissue Engineering
Jianqing Liang, Xinyang Li, Yateng Dong, Bing Zhao
Summary: Fetal tissue-derived organoids play a crucial role in studying embryonic development and modeling diseases, but the lack of human fetal tissue references and research models poses challenges. This article provides an overview of the strategies and applications of fetal tissue-derived organoids, focusing on their role in research on fetal organ development, developmental defect disease modeling, and organ-organ interaction. The discussion on the importance of fetal tissue research also highlights the prospects and challenges in this field.
CELL TRANSPLANTATION
(2022)
